Cited by 69Enzyme Production and CharacterizationSeaweed-derived Bioactive CompoundsMicrobial Community Ecology and Physiology

Isolation and Characterization of a Fucoidan-Degrading Marine Bacterial Strain and Its Fucoidanase

Sakai, Takeshi, Kawai, Takashi, Kato, Ikunoshin·2004·Marine Biotechnology

Abstract

A marine bacterial strain that degraded fucoidan from Kjellmaniella crassifolia (class Phaeophyceae, order Laminariales, family Laminariaceae) was isolated in our laboratory. The strain was gram-negative, ubiquinone 8 was the predominant respiratory quinone, and the GC-content of its genomic DNA was 36%. The cells of the strain were rod-shaped (2.0 microm long x 1.0 microm wide), and each cell was motile by means of one polar flagellum. Phylogenetic analysis of its 16S ribosomal DNA sequence indicated that it was a member of the family Alteromonadaceae. It produced a type of extracellular fucoidanase, an endosulfated fucan-digesting enzyme. The enzyme was purified with 3500-fold purity at 12.0% yield. Optimum conditions for the enzyme reaction were approximately pH 6.5 to 8.0 and temperature 30 degrees to 35 degrees C. The enzyme was activated by calcium ions, and maximum activity was observed in the presence of greater than 30 mM calcium ion.

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Crossref · 10.1007/s10126-003-0033-5

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Authors
Sakai, T., Kawai, T., & Kato, I.
Affiliations
Takara BIO Inc. (Japan) (JP)
Corresponding author
Sakai, Takeshi
Venue
Marine Biotechnology
Published
2004-06-23
Volume
6
Issue
4
Pages
335-346
ISSN
  • 1436-2228 (ISSN-L)
  • 1436-2236
OpenAlex
W1983888735
Cited by
69
References
45
Citations by year
2026: 1 · 2025: 5 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 4 · 2017: 9 · 2016: 1 · 2015: 5 · 2014: 2 · 2013: 6 · 2012: 3
FWCI
0.26
Type
journal article
Language
en
Open access
closed
Locations
  • Marine Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
Keywords
fucoidanase, marine bacteria, Alteromonadaceae, brown algae, extracellular enzymes, enzyme purification, calcium activation, bacterial isolation, 16S rRNA phylogeny
MeSH terms
Phaeophyceae, Base Composition, Base Sequence, Enzymes, Hydrogen-Ion Concentration, Molecular Sequence Data, Phylogeny, Polysaccharides, RNA, Ribosomal, 16S, Temperature, Cluster Analysis, Sequence Analysis, DNA, DNA Primers, Alteromonadaceae

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