Isolation and Characterization of a Fucoidan-Degrading Marine Bacterial Strain and Its Fucoidanase
Abstract
A marine bacterial strain that degraded fucoidan from Kjellmaniella crassifolia (class Phaeophyceae, order Laminariales, family Laminariaceae) was isolated in our laboratory. The strain was gram-negative, ubiquinone 8 was the predominant respiratory quinone, and the GC-content of its genomic DNA was 36%. The cells of the strain were rod-shaped (2.0 microm long x 1.0 microm wide), and each cell was motile by means of one polar flagellum. Phylogenetic analysis of its 16S ribosomal DNA sequence indicated that it was a member of the family Alteromonadaceae. It produced a type of extracellular fucoidanase, an endosulfated fucan-digesting enzyme. The enzyme was purified with 3500-fold purity at 12.0% yield. Optimum conditions for the enzyme reaction were approximately pH 6.5 to 8.0 and temperature 30 degrees to 35 degrees C. The enzyme was activated by calcium ions, and maximum activity was observed in the presence of greater than 30 mM calcium ion.
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Fucoidanase from marine bacterium Fucanobacter lyticus exhibits extracellular endo-fucoidanase degrading activity on fucoidan polysaccharides from brown seaweed in the order Laminariales but not Fucales and Chordariales, indicating specificity of an endo-1,3-α-fucoidanase.
“Fucoidanase from the marine bacterium Fucanobacter lyticus has shown to exhibit extracellular endo-fucoidanase degrading activity on fucoidan polysaccharides obtained from brown seaweed in the order of Laminariales but not Fucales and Chordariales indicating specificity of an endo-1,3-a-fucoidanase. 44”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [44]
Marine bacteria such as Fucophilus fucoidanolyticus are to a certain extent able to consume fucoidans of various structures and apparently synthesize a number of fucoidan-degrading enzymes.
“Marine bacteria such as Fucophilus fucoidanolyticus are to a certain extent able to consume fucoidans of various structures and apparently”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [44]
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- Authors
- Sakai, T., Kawai, T., & Kato, I.
- Affiliations
- Takara BIO Inc. (Japan) (JP)
- Corresponding author
- Sakai, Takeshi
- ORCID
- Sakai, Takeshi
- Venue
- Marine Biotechnology
- Published
- 2004-06-23
- Volume
- 6
- Issue
- 4
- Pages
- 335-346
- ISSN
- 1436-2228 (ISSN-L)
- 1436-2236
- OpenAlex
- W1983888735
- Cited by
- 69
- References
- 45
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 4 · 2017: 9 · 2016: 1 · 2015: 5 · 2014: 2 · 2013: 6 · 2012: 3
- FWCI
- 0.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Marine Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- fucoidanase, marine bacteria, Alteromonadaceae, brown algae, extracellular enzymes, enzyme purification, calcium activation, bacterial isolation, 16S rRNA phylogeny
- MeSH terms
- Phaeophyceae, Base Composition, Base Sequence, Enzymes, Hydrogen-Ion Concentration, Molecular Sequence Data, Phylogeny, Polysaccharides, RNA, Ribosomal, 16S, Temperature, Cluster Analysis, Sequence Analysis, DNA, DNA Primers, Alteromonadaceae
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