Production of the artemisinin precursor amorpha-4,11-diene by engineered Saccharomyces cerevisiae
Abstract
The gene encoding for amorpha-4,11-diene synthase from Artemisia annua was transformed into yeast Saccharomyces cerevisiae in two fundamentally different ways. First, the gene was subcloned into the galactose-inducible, high-copy number yeast expression vector pYeDP60 and used to transform the Saccharomyces cerevisiae strain CEN.PK113-5D. Secondly, amorpha-4,11-diene synthase gene, regulated by the same promoter, was introduced into the yeast genome by homologous recombination. In protein extracts from galactose-induced yeast cells, a higher activity was observed for yeast expressing the enzyme from the plasmid. The genome-transformed yeast grows at the same rate as wild-type yeast while plasmid-carrying yeast grows somewhat slower than the wild-type yeast. The plasmid and genome-transformed yeasts produced 600 and 100 microg/l of the artemisinin precursor amorpha-4,11-diene, respectively, during 16-days' batch cultivation.
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Both Saccharomyces cerevisiae and Escherichia coli have been used as hosts when attempting to establish a viable recombinant microbial pathway for artemisinin biosynthesis.
“Both Saccharomyces cerevisiae [379,380] and Escherichia coli [381,382,383] have been used as hosts when attempting to establish a viable recombinant microbial pathway for the biosynthesis of artemisinin.”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [379]
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- Authors
- Lindahl, A.-L., Olsson, M. E., Mercke, P., Tollbom, Ö., Schelin, J., Brodelius, M., & Brodelius, P. E.
- Corresponding author
- Brodelius, Peter E.
- Venue
- Biotechnology Letters
- Published
- 2006-04
- Volume
- 28
- Issue
- 8
- Pages
- 571-580
- ISSN
- 0141-5492 (ISSN-L)
- 1573-6776
- OpenAlex
- W2010367299
- Cited by
- 82
- References
- 23
- Citations by year
- 2024: 2 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 4 · 2013: 9 · 2012: 11
- FWCI
- 3.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biotechnology Letters (publishedVersion)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Funders
- Carl Tryggers Stiftelse för Vetenskaplig Forskning
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- amorpha-4,11-diene synthase, Artemisia annua, Saccharomyces cerevisiae, heterologous expression, homologous recombination, batch culture, sesquiterpene biosynthesis
- MeSH terms
- Genetic Engineering, Gas Chromatography-Mass Spectrometry, Plasmids, Polyisoprenyl Phosphates, Recombination, Genetic, Saccharomyces cerevisiae, Sesquiterpenes, Transformation, Genetic, Blotting, Western, Molecular Structure, Genome, Fungal, Alkyl and Aryl Transferases, Artemisia annua, Artemisinins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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