The improvement of amorpha-4,11-diene production by a yeast-conform variant
Abstract
AIMS: To investigate the effect of the yeast-conform variant of the Artemisia annua gene encoding for amorpha-4,11-diene synthase (ADS) on the production of amorpha-4,11-diene in a transformed yeast. METHODS AND RESULTS: The ADS gene was mutated to the yeast-conform variant ADSm. The ADSm synthesis was performed based on step-by-step extension of a short region of the gene through a series of polymerase chain reactions (PCR). The artificial ADSm gene contained codons preferred by the yeast translation machinery. The sequence was then integrated into a yeast expression vector pYeDP60. The fusion construct was active and the transformed yeast cells produced higher level of amorpha-4,11-diene compared with the plant gene-transformed yeast cells. CONCLUSIONS: Strains transformed with the yeast-conform allele (ADSm) were more efficient in terms of production of amorpha-4,11-diene than those transformed with the plant gene. SIGNIFICANCE AND IMPACT OF THE STUDY: We demonstrated that yeast-conform allele of foreign genes by serial PCR reactions can be a solution to low efficiency of heterologous gene expression in Saccharomyces cerevisiae cells.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Investigators have concentrated on expressing enzymes from phases 1 and 2 of the artemisinin biosynthetic route in transgenic organisms, including HMGR, FPPS, ADS, and CYP71AV1.
“To date, investigators have concentrated on expressing the enzymes from phases 1 and 2 of the biosynthetic route to artemisinin in transgenic organisms (see Scheme 9 in Section 3). These have included: HMG-CoA reductase (HMGR) [370]; farnesyl diphosphate synthase (FPPS) [371, 372,373,374]; amorpha-4,11-diene synthase (ADS) [375,376,377]; and the P450 enzyme which oxidises amorpha-4,11-diene to art”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [375]
Cites
Sharp, P. M., & Li, W.-H. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.3.1281 · Cited by 3,992 · see citing works
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Ro, D.-K., Paradise, E. M., Ouellet, M., Fisher, K. J., Newman, K. L., Ndungu, J. M., Ho, K. A., Eachus, R. A., Ham, T. S., Kirby, J., et al. (2006) · Nature · doi:10.1038/nature04640 · Cited by 2,805 · see citing works
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
Gietz, R. D., Schiestl, R. H., Willems, A. R., & Woods, R. A. (1995) · Yeast · doi:10.1002/yea.320110408 · Cited by 2,030 · see citing works
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
Martin, V. J. J., Pitera, D. J., Withers, S. T., Newman, J. D., & Keasling, J. D. (2003) · Nature Biotechnology · doi:10.1038/nbt833 · Cited by 1,720 · see citing works
Codon usage in bacteria: correlation with gene expressivity.
Gouy, M., & Gautier, C. (1982) · Nucleic Acids Research · doi:10.1093/nar/10.22.7055 · Cited by 1,182 · see citing works
[6] Yeast expression of animal and plant P450s in optimized redox environments
Pompon, D., Louërat-Oriou, B., Bronine, A., & Urban, P. (1996) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(96)72008-6 · Cited by 654 · see citing works
Teoh, K. H., Polichuk, D. R., Reed, D. W., Nowak, G., & Covello, P. S. (2006) · FEBS Letters · doi:10.1016/j.febslet.2006.01.065 · Cited by 409 · see citing works
Schmid, G., & Hofheinz, W. (1983) · Journal of the American Chemical Society · doi:10.1021/ja00341a054 · Cited by 329 · cited 2× · see citing works
“Several approaches have been developed to generate artemisinin, including extracting from plants of A. annua L (Coordinating Group for Research on the Structure of Qinghaosu 1977), total synthesis (Schmid and Hofheinz 1983; Avery et al. 1992) and tissue or cell cultures (Liu et al. 1997).”
BackgroundEfferth, T. (2007) · Planta Medica · doi:10.1055/s-2007-967138 · Cited by 269 · see citing works
Amorpha-4,11-diene synthase catalyses the first probable step in artemisinin biosynthesis
Bouwmeester, H. J., Wallaart, T. E., Janssen, M. H. A., van Loo, B., Jansen, B. J. M., Posthumus, M. A., Schmidt, C. O., De Kraker, J.-W., König, W. A., & Franssen, M. C. R. (1999) · Phytochemistry · doi:10.1016/s0031-9422(99)00206-x · Cited by 266 · see citing works
Newman, J. D., Marshall, J., Chang, M., Nowroozi, F., Paradise, E., Pitera, D., Newman, K. L., & Keasling, J. D. (2006) · Biotechnology and Bioengineering · doi:10.1002/bit.21017 · Cited by 257 · see citing works
Artemisinin, a Novel Antimalarial Drug: Biochemical and Molecular Approaches for Enhanced Production
Abdin, M. Z., Israr, M., Rehman, R. U., & Jain, S. K. (2003) · Planta Medica · doi:10.1055/s-2003-38871 · Cited by 252 · see citing works
Avery, M. A., Chong, W. K. M., & Jennings-White, C. (1992) · Journal of the American Chemical Society · doi:10.1021/ja00029a028 · Cited by 250 · cited 1× · see citing works
“Several approaches have been developed to generate artemisinin, including extracting from plants of A. annua L (Coordinating Group for Research on the Structure of Qinghaosu 1977), total synthesis (Schmid and Hofheinz 1983; Avery et al. 1992) and tissue or cell cultures (Liu et al. 1997).”
BackgroundWallaart, T. E., Bouwmeester, H. J., Hille, J., Poppinga, L., & Maijers, N. C. A. (2001) · Planta · doi:10.1007/s004250000428 · Cited by 242 · see citing works
Bertea, C. M., Freije, J. R., van der Woude, H., Verstappen, F. W., Perk, L., Marquez, V., De Kraker, J.-W., Posthumus, M. A., Jansen, B. J., de Groot, A., et al. (2005) · Planta Medica · doi:10.1055/s-2005-837749 · Cited by 205 · see citing works
Denis, C. L., Ferguson, J., & Young, E. T. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)33174-0 · Cited by 189 · see citing works
Low-usage codons in Escherichia coli, yeast, fruit fly and primates
Zhang, S., Zubay, G., & Goldman, E. (1991) · Gene · doi:10.1016/0378-1119(91)90514-c · Cited by 188 · see citing works
Didierjean, L., Gondet, L., Perkins, R., et al. (2002) · PLANT PHYSIOLOGY · doi:10.1104/pp.005801 · Cited by 162 · see citing works
Sinclair, G. B., & Choy, F. Y. M. (2002) · Protein Expression and Purification · doi:10.1016/s1046-5928(02)00526-0 · Cited by 159 · see citing works
Chang, Y.-J., Song, S.-H., Park, S.-H., & Kim, S.-U. (2000) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.2000.2061 · Cited by 154 · see citing works
Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua
Matsushita, Y., Kang, W., & Charlwood, B. V. (1996) · Gene · doi:10.1016/0378-1119(96)00054-6 · Cited by 95 · see citing works
Production of artemisinin by hairy root cultures of Artemisia annua L
Liu, C. Z., Wang, Y. C., Ouyang, F., Ye, H. C., & Li, G. F. (1997) · Biotechnology Letters · doi:10.1023/a:1018362309677 · Cited by 94 · see citing works
Production of the artemisinin precursor amorpha-4,11-diene by engineered Saccharomyces cerevisiae
Lindahl, A.-L., Olsson, M. E., Mercke, P., Tollbom, Ö., Schelin, J., Brodelius, M., & Brodelius, P. E. (2006) · Biotechnology Letters · doi:10.1007/s10529-006-0015-6 · Cited by 82 · see citing works
Wallaart, T. E., Pras, N., & Quax, W. J. (1999) · Planta Medica · doi:10.1055/s-1999-14094 · Cited by 81 · see citing works
Thomson, J. M., & Parrott, W. A. (1998) · BioTechniques · doi:10.2144/98246bm04 · Cited by 56 · see citing works
Li, Z., Liu, Y., Liu, B., Wang, H., Ye, H., & Li, G. (2006) · Journal of Integrative Plant Biology · doi:10.1111/j.1744-7909.2006.00381.x · Cited by 23 · see citing works
Kong, J., Cheng, K.-D., Wang, L., Zheng, X., Dai, J.-G., Zhu, P., & Wang, W. (2007) · PubMed · Cited by 6 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao)
Brown, G. D. (2010) · Molecules · doi:10.3390/molecules15117603 · Cited by 363 · see citing works
Xie, D.-Y., Ma, D.-M., Judd, R., & Jones, A. L. (2016) · Phytochemistry Reviews · doi:10.1007/s11101-016-9480-2 · Cited by 63 · see citing works
- Authors
- Jiang-Qiang, K., Wei, W., Li-Na, W., Xiao-Dong, Z., Ke-Di, C., & Ping, Z.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Corresponding author
- Jiang-Qiang, K.
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-03
- Volume
- 106
- Issue
- 3
- Pages
- 941-951
- ISSN
- 1364-5072 (ISSN-L)
- 1365-2672
- OpenAlex
- W2066361534
- Cited by
- 22
- References
- 27
- Citations by year
- 2024: 3 · 2022: 1 · 2021: 1 · 2017: 1 · 2016: 2 · 2013: 3 · 2012: 4
- FWCI
- 1.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- amorpha-4,11-diene synthase, Artemisia annua, heterologous expression, Saccharomyces cerevisiae, terpenoid biosynthesis
- MeSH terms
- Polycyclic Sesquiterpenes, Genetic Engineering, Gas Chromatography-Mass Spectrometry, Molecular Sequence Data, Plasmids, Recombination, Genetic, Saccharomyces cerevisiae, Sesquiterpenes, Transformation, Genetic, Polymerase Chain Reaction, Genome, Fungal, Alkyl and Aryl Transferases, Artemisia annua
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
