Cited by 66Neuroinflammation and Neurodegeneration MechanismsFatty Acid Research and HealthAdipokines, Inflammation, and Metabolic Diseases

Exposure to the Saturated Free Fatty Acid Palmitate Alters BV-2 Microglia Inflammatory Response

Tracy, Linda M., Bergqvist, Filip, Ivanova, Elena V., Jacobsen, Kristin T., Iverfeldt, Kerstin·2013·Journal of Molecular Neuroscience

Abstract

Elevated levels of free fatty acids (FFAs) in plasma and increased incidence of chronic systemic inflammation are associated with obesity. In the brain, activated microglia are believed to play different roles during inflammation that may either be neuroprotective or promote neurodegeneration. Here, we have investigated the effects of FFAs on microglial response to inflammatory stimuli. Our results indicate that the saturated FFA palmitate on its own induces alternative activation of BV-2 microglia cells. Further, pre-exposure to palmitate changed the response of microglia to lipopolysaccharide (LPS). We show that palmitate affects the mRNA levels of the pro-inflammatory cytokines interleukin-1β and interleukin-6. The transcription factor CCAAT/enhancer-binding protein δ is also affected by pre-exposure to palmitate. Furthermore, the phagocytic activity of microglia was investigated using fluorescent beads. By analyzing the bead uptake by fluorescence-activated cell sorting, we found that palmitate alone, as well as together with LPS, stimulated the phagocytic activity of microglia. Taken together, our results suggest that exposure of microglia to increased levels of free fatty acids may alter the consequences of classical inflammatory stimuli.

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Crossref · 10.1007/s12031-013-0068-7

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Authors
Tracy, L. M., Bergqvist, F., Ivanova, E. V., Jacobsen, K. T., & Iverfeldt, K.
Affiliations
Stockholm University (SE)
Corresponding author
Iverfeldt, Kerstin
Venue
Journal of Molecular Neuroscience
Published
2013-07-25
Volume
51
Issue
3
Pages
805-812
ISSN
  • 0895-8696 (ISSN-L)
  • 1559-1166
OpenAlex
W2118974366
Cited by
63
References
31
Citations by year
2026: 1 · 2025: 2 · 2024: 7 · 2023: 4 · 2022: 10 · 2021: 9 · 2020: 5 · 2019: 7 · 2018: 3 · 2017: 6 · 2016: 3 · 2015: 2 · 2014: 4
FWCI
1.68
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Molecular Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
palmitate, free fatty acids, microglia, BV-2 cells, alternative activation, lipopolysaccharide, neuroinflammation, IL-1β, interleukin-6, C/EBPδ, phagocytosis, obesity, chronic systemic inflammation
MeSH terms
Animals, Cell Line, Lipopolysaccharides, Phagocytosis, RNA, Messenger, Cytokines, Microglia, Palmitic Acid, Mice, CCAAT-Enhancer-Binding Protein-delta

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