Evaluating the probiotic potential of Lactobacillus plantarum strains from Algerian infant feces: Towards the design of probiotic starter cultures tailored for developing countries
Abstract
Lactobacilli naturally present in the neonatal gut are believed to be beneficial for the human hosts and are investigated as potential probiotics. In this study, we aimed to characterize six Lactobacillus plantarum strains derived from the feces of a breast-fed infant, for the development of new probiotic cultures. Our attention was focused on L. plantarum in reason of the presence, within such species, of both pro-technological and probiotic strains, i.e., a combination of particular interest to design tailored probiotic starter cultures for developing countries. The bacterial isolates exhibiting lactobacilli-like phenotypic characteristics were identified as members of the L. plantarum group by 16S rRNA gene sequencing, and their diversity was evaluated by randomly amplified polymorphic DNA (RAPD) PCR patterns. The selected strains were screened for probiotic potential through in vitro tests. Firstly, bacterial survival was evaluated in an in vitro system simulating the human oro-gastrointestinal tract, using also milk as a carrier matrix. Besides, physiological traits such as antibiotic susceptibility, antimicrobial activity against selected enteric pathogens, and adhesion to abiotic surfaces and to gastric mucin were studied. Considering the resistance to simulated gastrointestinal digestion and the results from the biofilm and mucin adhesion tests, a strain-denominated L. plantarum LSC3 was selected for further evaluation of in vitro adhesion ability to intestinal mucosa and immunomodulatory activities. L. plantarum LSC3 was able to adhere efficiently to human enterocyte-like cells (Caco-2 cells), and decreased IL-8 transcription while increasing IL-10 mRNA level, as revealed by transcriptional analysis on LPS-stimulated human (THP-1) macrophages. Our results highlight that L. plantarum LSC3 fulfills major in vitro probiotic criteria as well as interesting immunostimulatory properties, and thus may be a promising candidate for further in vivo studies aiming at the development of novel probiotic starter cultures.
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Probiotics could exert competitive inhibition against pathogens and confer antagonism against potentially harmful microorganisms, thereby preventing colonization of the host mucosa.
“It is known that probiotics could exert competitive inhibition against pathogens. Moreover, it can confer antagonism against potentially harmful microorganisms, thereby preventing colonization of the host mucosa [52].”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [52]
Cites
The human microbiome: at the interface of health and disease
Cho, I., & Blaser, M. J. (2012) · Nature Reviews Genetics · doi:10.1038/nrg3182 · Cited by 3,449 · see citing works
One Hundred and Twenty-Seven Cultured Human Tumor Cell Lines Producing Tumors in Nude Mice23
Fogh, J., Fogh, J. M., & Orfeo, T. (1977) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/59.1.221 · Cited by 1,599 · see citing works
Complete genome sequence of <i>Lactobacillus plantarum</i> WCFS1
Kleerebezem, M., Boekhorst, J., van Kranenburg, R., Molenaar, D., Kuipers, O. P., Leer, R., Tarchini, R., Peters, S. A., Sandbrink, H. M., Fiers, M. W. E. J., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0337704100 · Cited by 1,472 · see citing works
Diet, gut microbiota and immune responses
Maslowski, K. M., & Mackay, C. R. (2011) · Nature Immunology · doi:10.1038/ni0111-5 · Cited by 1,261 · see citing works
Genes and Molecules of Lactobacilli Supporting Probiotic Action
Lebeer, S., Vanderleyden, J., & De Keersmaecker, S. C. J. (2008) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00017-08 · Cited by 1,013 · see citing works
Human milk is a source of lactic acid bacteria for the infant gut
Martín, R., Langa, S., Reviriego, C., Jimínez, E., Marín, M. L., Xaus, J., Fernández, L., & Rodríguez, J. M. (2003) · The Journal of Pediatrics · doi:10.1016/j.jpeds.2003.09.028 · Cited by 792 · see citing works
Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests
Argyri, A. A., Ζουμποπούλου, Γ., Karatzas, K. A. G., Tsakalidou, E., Nychas, G. E., Panagou, E. Ζ., & Tassou, C. C. (2012) · Food Microbiology · doi:10.1016/j.fm.2012.10.005 · Cited by 659 · see citing works
Resta-Lenert, S., & Barrett, K. E. (2003) · Gut · doi:10.1136/gut.52.7.988 · Cited by 642 · see citing works
Intestinal Bacteria and the Regulation of Immune Cell Homeostasis
Hill, D. A., & Artis, D. (2010) · Annual Review of Immunology · doi:10.1146/annurev-immunol-030409-101330 · Cited by 586 · see citing works
Food and Agricultural Organization of the United Nations.
Leung, W. T. W. (1962) · Journal of AOAC INTERNATIONAL · doi:10.1093/jaoac/45.2.508a · Cited by 542 · see citing works
Lactobacillus plantarum and Its Probiotic and Food Potentialities
Seddik, H. A., Bendali, F., Gancel, F., Fliss, I., Spano, G., & Drider, D. (2017) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-017-9264-z · Cited by 420 · see citing works
Self-citationBiofilm Forming Lactobacillus: New Challenges for the Development of Probiotics
Salas-Jara, M., Ilabaca, A., Vega, M. A., & García, A. (2016) · Microorganisms · doi:10.3390/microorganisms4030035 · Cited by 353 · see citing works
Recent advances in inflammatory bowel disease: mucosal immune cells in intestinal inflammation
Cader, M. Z., & Kaser, A. (2013) · Gut · doi:10.1136/gutjnl-2012-303955 · Cited by 337 · see citing works
Survival and activity of selected probiotic organisms in set-type yoghurt during cold storage
Donkor, O. N., Nilmini, S. L. I., Stolic, P., Vasiljevic, T., & Shah, N. P. (2006) · International Dairy Journal · doi:10.1016/j.idairyj.2006.08.006 · Cited by 333 · see citing works
Arena, M. P., Silvain, A., Normanno, G., et al. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00464 · Cited by 297 · see citing works
Self-citationSiezen, R. J., Tzeneva, V. A., Castioni, A., Wels, M., Phan, H. T. K., Rademaker, J. L. W., Starrenburg, M. J. C., Kleerebezem, M., Douwe Molenaar, B., & Vlieg, J. E. T. V. H. (2009) · Environmental Microbiology · doi:10.1111/j.1462-2920.2009.02119.x · Cited by 293 · see citing works
Ducrotte, P. R. (2012) · World Journal of Gastroenterology · doi:10.3748/wjg.v18.i30.4012 · Cited by 273 · see citing works
Russo, P., Arena, M. P., Fiocco, D., Capozzi, V., Drider, D., & Spano, G. (2017) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2016.04.027 · Cited by 272 · see citing works
Self-citationExploringLactobacillus plantarumGenome Diversity by Using Microarrays
Douwe Molenaar, B., Bringel, F., Schuren, F. H. J., de Vos, W. M., Siezen, R. J., & Kleerebezem, M. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.17.6119-6127.2005 · Cited by 252 · see citing works
Assessment of probiotic properties in lactic acid bacteria isolated from wine
García‐Ruiz, A., de Llano, D. G., Esteban‐Fernández, A., Requena, T., Bartolomé, B., & Moreno-Arribas, M. V. (2014) · Food Microbiology · doi:10.1016/j.fm.2014.06.015 · Cited by 241 · see citing works
Food Formats for Effective Delivery of Probiotics
Sanders, M. E., & Marco, M. L. (2010) · Annual Review of Food Science and Technology · doi:10.1146/annurev.food.080708.100743 · Cited by 217 · see citing works
Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-κB activation
Riedel, C. U. (2006) · World Journal of Gastroenterology · doi:10.3748/wjg.v12.i23.3729 · Cited by 210 · see citing works
Vergara-Irigaray, M., Valle, J., Merino, N., et al. (2009) · Infection and Immunity · doi:10.1128/iai.00616-09 · Cited by 206 · see citing works
Lomax, A. E., & Calder, P. C. (2009) · Current Pharmaceutical Design · doi:10.2174/138161209788168155 · Cited by 203 · see citing works
MacKenzie, D. A., Jeffers, F., Parker, M. L., Vibert-Vallet, A., Bongaerts, R. J., Roos, S., Walter, J., & Juge, N. (2010) · Microbiology · doi:10.1099/mic.0.043265-0 · Cited by 202 · see citing works
Castellano, P., Belfiore, C., Fadda, S., & Vignolo, G. M. (2007) · Meat Science · doi:10.1016/j.meatsci.2007.10.009 · Cited by 200 · see citing works
Beta-Glucans Improve Growth, Viability and Colonization of Probiotic Microorganisms
Russo, P., López, P., Capozzi, V., De Palencia, P. F., Dueñas, M. T., Spano, G., & Fiocco, D. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13056026 · Cited by 176 · see citing works
Self-citationEffect of prebiotic carbohydrates on the growth and tolerance of Lactobacillus
Hernández‐Hernández, O., Muthaiyan, A., Moreno, F. J., Montilla, A., Sanz, M. L., & Ricke, S. C. (2012) · Food Microbiology · doi:10.1016/j.fm.2011.12.022 · Cited by 171 · see citing works
Wang, C.-Y., Lin, P.-R., Ng, C.-C., & Shyu, Y.-T. (2010) · Anaerobe · doi:10.1016/j.anaerobe.2010.10.003 · Cited by 155 · see citing works
Buriti, F. C. A., de Castro, I. A., & Saad, S. M. I. (2009) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2009.11.030 · Cited by 151 · see citing works
Helwig, U. (2006) · World Journal of Gastroenterology · doi:10.3748/wjg.v12.i37.5978 · Cited by 124 · see citing works
Barley β-Glucans-Containing Food Enhances Probiotic Performances of Beneficial Bacteria
Arena, M. P., Caggianiello, G., Fiocco, D., Russo, P., Torelli, M., Spano, G., & Capozzi, V. (2014) · International Journal of Molecular Sciences · doi:10.3390/ijms15023025 · Cited by 123 · see citing works
Self-citationTechnological properties of Lactobacillus plantarum strains isolated from grape must fermentation
Berbegal, C., Peña, N., Russo, P., Grieco, F., Pardo, I., Ferrer, S., Spano, G., & Capozzi, V. (2016) · Food Microbiology · doi:10.1016/j.fm.2016.03.002 · Cited by 112 · see citing works
Self-citationBove, P., Russo, P., Capozzi, V., Gallone, A., Spano, G., & Fiocco, D. (2013) · Microbiological Research · doi:10.1016/j.micres.2013.01.004 · Cited by 105 · see citing works
Self-citationde Palencia, P. F., López, P., Corbí, A. L., Peláez, C., & Requena, T. (2008) · European Food Research and Technology · doi:10.1007/s00217-008-0870-6 · Cited by 103 · see citing works
Rong, J., Zheng, H., Liu, M., Hu, X., Wang, T., Zhang, X., Jin, F., & Wang, L. (2015) · BMC Microbiology · doi:10.1186/s12866-015-0525-2 · Cited by 99 · see citing works
Tulini, F. L., Winkelströter, L. K., & Martinis, E. C. P. D. (2013) · Anaerobe · doi:10.1016/j.anaerobe.2013.06.006 · Cited by 93 · see citing works
Monika, Savitri, Kumar, V., Kumari, A., Angmo, K., & Bhalla, T. C. (2017) · Journal of Food Science and Technology · doi:10.1007/s13197-017-2629-1 · Cited by 93 · see citing works
In vitro evaluation of the probiotic potential of Lactobacillus salivarius SMXD51
Messaoudi, S., Madi, A., Prévost, H., Feuilloley, M. G. J., Manaï, M., Dousset, X., & Connil, N. (2012) · Anaerobe · doi:10.1016/j.anaerobe.2012.10.004 · Cited by 90 · see citing works
Arena, M. P., Capozzi, V., Spano, G., & Fiocco, D. (2017) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-017-8182-z · Cited by 86 · see citing works
Self-citationTerraf, M. C. L., Mendoza, L. M., Tomás, M. S. J., Silva, C. N., & Nader-Macı́as, M. E. (2014) · Journal of Applied Microbiology · doi:10.1111/jam.12642 · Cited by 80 · see citing works
Probiotic features of Lactobacillus plantarum mutant strains
Bove, P., Gallone, A., Russo, P., Capozzi, V., Albenzio, M., Spano, G., & Fiocco, D. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4031-2 · Cited by 78 · see citing works
Self-citationProbiotic attributes ofLactobacillusstrains isolated from food and of human origin
Gaudana, S. B., Dhanani, A. S., & Bagchi, T. (2010) · British Journal Of Nutrition · doi:10.1017/s0007114509993643 · Cited by 76 · see citing works
Probiotic Characteristics of Lactobacillus curvatus DN317, a Strain Isolated from Chicken Ceca
Zommiti, M., Connil, N., Hamida, J. B., & Ferchichi, M. (2017) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-017-9301-y · Cited by 67 · see citing works
Adhesion ofLactobacilliand their anti-infectivity potential
Yadav, A. K., Tyagi, A., Kumar, A., Panwar, S., Grover, S., Saklani, A. C., Rajkumar, H., & Batish, V. K. (2015) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2014.918533 · Cited by 64 · see citing works
Development of intestinal bifidobacteria and lactobacilli in breast-fed neonates
Chen, J., Cai, W., & Feng, Y. (2007) · Clinical Nutrition · doi:10.1016/j.clnu.2007.03.003 · Cited by 59 · see citing works
Seddik, H. A., Bendali, F., Cudennec, B., & Drider, D. (2017) · Research in Microbiology · doi:10.1016/j.resmic.2016.12.003 · Cited by 57 · see citing works
Locally sourced probiotics, the next opportunity for developing countries?
Sybesma, W., Kort, R., & Lee, Y. K. (2015) · Trends in biotechnology · doi:10.1016/j.tibtech.2015.01.002 · Cited by 55 · see citing works
Immunomodulatory activity of two potential probiotic strains in LPS-stimulated HT-29 cells
Duary, R. K., Batish, V. K., & Grover, S. (2014) · Genes & Nutrition · doi:10.1007/s12263-014-0398-2 · Cited by 52 · see citing works
Arena, M. P., Russo, P., Capozzi, V., Rascón, A., Felis, G. E., Spano, G., & Fiocco, D. (2016) · Journal of Functional Foods · doi:10.1016/j.jff.2016.02.015 · Cited by 48 · see citing works
Self-citationProbiotics for the Developing World
Reid, G., Anand, S. K., Bingham, M. O., Mbugua, G. G., Wadström, T., Fuller, R., Anukam, K. C., & Katsivo, M. (2005) · Journal of Clinical Gastroenterology · doi:10.1097/01.mcg.0000165648.32371.38 · Cited by 46 · see citing works
Bosch, M., Rodriguez, M., Garcia, F., Fernández, E., Fuentes, M. C., & Cuñé, J. (2011) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2011.03199.x · Cited by 41 · see citing works
Characterization of Lactobacillus plantarum from wine must by PCR species-specific and RAPD-PCR
Spano, G., Beneduce, L., Tarantino, D., et al. (2002) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.2002.01200.x · Cited by 41 · see citing works
Self-citationBäuerl, C., Llopis, M., Antolı́n, M., Monedero, V., Mata, M., Zúñiga, M., Guarner, F., & Pérez‐Martínez, G. (2012) · Genes & Nutrition · doi:10.1007/s12263-012-0301-y · Cited by 38 · see citing works
Gu, C. T., Li, C. Y., Yang, L. J., & Huo, G. C. (2013) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.054296-0 · Cited by 38 · see citing works
Functional Starters for Functional Yogurt
Arena, M. P., Caggianiello, G., Russo, P., Albenzio, M., MASSA, S., Fiocco, D., Capozzi, V., & Spano, G. (2015) · Foods · doi:10.3390/foods4010015 · Cited by 35 · see citing works
Self-citationCharacterization ofLactobacillus gasseriisolates from a breast-fed infant
da Cunha, L. R., Ferreira, C. L. D. L. F., Durmaz, E., Goh, Y. J., Sanozky-Dawes, R., & Klaenhammer, T. R. (2012) · Gut Microbes · doi:10.4161/gmic.19489 · Cited by 35 · see citing works
Ziarno, M., & Zaręba, D. (2015) · Microbial Ecology in Health and Disease · doi:10.3402/mehd.v26.27812 · Cited by 30 · see citing works
Human studies on probiotics and endogenous lactic acid bacteria in the urogenital tract
Reid, G., Anand, S. K., Bingham, M. O., Mbugua, G. G., Wadström, T., Fuller, R., Anukam, K. C., & Katsivo, M. (2011) · Scholarship@Western (Western University) · Cited by 26 · see citing works
Screening and characterization of lactic acid bacteria from fermented milk.
Lavanya, B., Sowmiya, S., Balaji, S., & Muthuvelan, B. (2011) · British Journal of Dairy Sciences · Cited by 25 · see citing works
Silva, B. C., Sandes, S. H. C., Alvim, L. B., Bomfim, M. R. Q., Nicoli, J. R., Neumann, E., & Nunes, Á. C. (2016) · Journal of Applied Microbiology · doi:10.1111/jam.13339 · Cited by 24 · see citing works
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Simões, L., Fernandes, N., de Souza, A., dos Santos, L., Magnani, M., Abrunhosa, L., Teixeira, J., Schwan, R. F., & Dias, D. R. (2022) · Fermentation · doi:10.3390/fermentation8060277 · Cited by 23 · see citing works
- Authors
- Gheziel, C., Russo, P., Arena, M. P., Spano, G., Ouzari, H.-I., Kheroua, O., Saidi, D., Fiocco, D., Kaddouri, H., & Capozzi, V.
- Affiliations
- Université Oran 1 Ahmed Ben Bella (DZ)
- University of Foggia (IT)
- Tunis University (TN)
- Corresponding author
- Fiocco, Daniela
- ORCID
- Venue
- Probiotics and Antimicrobial Proteins
- Published
- 2018-02-19
- Volume
- 11
- Issue
- 1
- Pages
- 113-123
- ISSN
- 1867-1306 (ISSN-L)
- 1867-1314
- OpenAlex
- W2790171455
- Cited by
- 68
- References
- 61
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 8 · 2023: 8 · 2022: 11 · 2021: 12 · 2020: 8 · 2019: 8 · 2018: 2
- FWCI
- 7.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Probiotics and Antimicrobial Proteins (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Regione Puglia
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus plantarum, probiotic starter cultures, infant feces, breast-fed infants, gastrointestinal survival, antimicrobial activity, Caco-2 cells, immunomodulation, IL-10, interleukin-8, enteric pathogens, biofilm formation
- MeSH terms
- Algeria, Bacterial Adhesion, Developing Countries, Feces, Humans, Immunologic Factors, Infant, Intestinal Mucosa, Biofilms, Caco-2 Cells, Probiotics, Lactiplantibacillus plantarum
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