Probiotic and anti-inflammatory attributes of an isolate Lactobacillus helveticus NS8 from Mongolian fermented koumiss
Open accessAbstract
BACKGROUND: Koumiss is a traditionally fermented mare's milk described with health-promoting potentials for decades. However, only a few studies focused on the probiotic strains isolated from koumiss. In this study, we collected koumiss samples from Inner Mongolian pasturing area of China and selected a promising strain of Lactobacillus helveticus, isolate NS8, based on the survival abilities in gastrointestinal tract (GIT) and adhesion to intestinal endothelial cells in vitro. As the ability to positively modulate host immune response is a feature of increasing importance in measuring the probiotic potential of a bacterial strain, our study mainly focus on the immunomodulatory properties of L. helveticus NS8 by using in vivo and ex vivo analyses. RESULTS: L. helveticus NS8 was identified by molecular-typing methods, both at genus and species levels. As a typical food niche-specific bacteria, NS8 showed a moderate survival ability in GIT environment in vitro. However, an excellent binding capacity to the human intestinal epithelial cells, along with significant autoaggregation and cell-surface hydrophobicity was observed. Additionally, the presence of S-layer protein was responsible for the cell surface properties of this strain. NS8 was found to be rather protective against TNBS (2,4,6-trinitrobenzene sulfonic acid)-induced murine colitis. In the meantime, co-culture with NS8 induced an increased level of secretion of anti-inflammatory cytokine IL-10 in peripheral blood mono-nuclear cells (PBMCs). Furthermore, NS8 was also able to diminish the proinflammatory effects of lipopolysaccharide (LPS) in mouse macrophage cell line RAW264.7 by inducing higher levels of IL-10. Specially, adding of the purified S-layer protein didn't influence the production of IL-10. The specific ligand-host receptor interactions on the NS8 specific immune responses need to be learned further. CONCLUSION: In summary, L. helveticus NS8 exhibited good probiotic and particularly immunomodulatory properties, with a potential for development of functional food commercially or therapeutic adjuvant for inflammatory diseases.
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Koumiss health benefits include a decrease in intestinal and pancreatic lipase activity, an impact on intestinal immune function in immunocompromised conditions, and a reduction in total cholesterol and LDL-cholesterol.
“The health benefits include a decrease in intestinal and pancreatic lipase activity, an impact on intestinal immune function in immunocompromised conditions, and a reduction in total cholesterol and LDL-cholesterol [193].”
From The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties (2024) · cites this paper as [193]
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“These results are in agreement with other studies on L. helveticus [28], as well as for other lactobacilli [28,38,40,41].”
BackgroundResult
- Authors
- Rong, J., Zheng, H., Liu, M., Hu, X., Wang, T., Zhang, X., Jin, F., & Wang, L.
- Affiliations
- Hangzhou Normal University (CN)
- Chinese Academy of Sciences (CN)
- Corresponding author
- Jin, Feng
- Wang, Li
- Venue
- BMC Microbiology
- Published
- 2015-10-02
- Volume
- 15
- Issue
- 1
- Pages
- 196-196
- ISSN
- 1471-2180 (ISSN-L)
- PMC
- PMC4591576
- OpenAlex
- W2129763947
- Cited by
- 99
- References
- 41
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 13 · 2023: 11 · 2022: 13 · 2021: 7 · 2020: 10 · 2019: 11 · 2018: 12 · 2017: 10 · 2016: 3
- FWCI
- 11.15
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- BMC Microbiology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Repository of Institute of Psychology, Chinese Academy of Sciences (Institute of Psychology, Chinese Academy of Sciences) (submittedVersion)
- Institutional Repository of Institute of Psychology, Chinese Academy of Sciences (Institute of Psychology, Chinese Academy of Sciences) (submittedVersion, cc-by-nc-sa)
- Funders
- National Natural Science Foundation of China (81101560, 81472659)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Animal Diversity and Health Studies
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- koumiss, probiotic potential, immunomodulation, anti-inflammatory activity, S-layer proteins, intestinal epithelial cell adhesion, gastrointestinal survival, self-aggregation, cell surface hydrophobicity, TNBS-induced colitis, IL-10, lipopolysaccharide-induced inflammation, macrophages, fermented mare's milk, functional foods
- MeSH terms
- RAW 264.7 Cells, Animals, Anti-Inflammatory Agents, Bacterial Adhesion, Cells, Cultured, Colitis, Disease Models, Animal, Humans, Leukocytes, Mononuclear, Membrane Glycoproteins, Mongolia, Trinitrobenzenesulfonic Acid, Interleukin-10, Caco-2 Cells, Probiotics, Gastrointestinal Tract, Cultured Milk Products, Mice, Lactobacillus helveticus, Molecular Typing
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