Central and peripheral release of vasopressin and oxytocin in the conscious rat after osmotic stimulation
Abstract
Vasopressin (AVP) and oxytocin (OXT) were measured by radioimmunoassay in push-pull perfusates and tissue samples of various brain areas, plasma and cerebrospinal fluid (CSF) of male rats in response to osmotic stimulation. Hypertonic saline caused a significant rise in plasma AVP and OXT and different changes in peptide contents, in the septum and hippocampus at 30 and 60 min after intraperitoneal injection. Push-pull perfusion (20 microliters artificial CSF/min, 30-min periods) of the septum and dorsal hippocampus of conscious, unrestrained animals revealed a significant, stimulus-evoked release of both AVP and OXT. This release was: (1) not always reflected by corresponding changes in the regional peptide content; (2) simultaneous with the peripheral release from the posterior pituitary; and (3) probably the result of synaptic/parasynaptic events as suggested by use of agents in the artificial CSF which either inhibit or facilitate the release from intact fibre terminals.
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Depolarizing stimuli can induce OT and AVP release in a calcium-dependent manner in brain regions where AVP and OT fibers terminate synaptically.
“In addition, depolarizing stimuli can induce OT and AVP release in a calcium-dependent manner in brain regions where AVP and OT fibers terminate synaptically [105; 106; 107].”
From Cross-talk among oxytocin and arginine-vasopressin receptors: Relevance for basic and clinical studies of the brain and periphery (2018) · cites this paper as [107]
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- Authors
- Landgraf, R., Neumann, I., & Schwarzberg, H.
- Affiliations
- Leipzig University (DE)
- Corresponding author
- Landgraf, R.
- ORCID
- Neumann, Inga
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1988-08
- Volume
- 457
- Issue
- 2
- Pages
- 219-225
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W1973592879
- Cited by
- 137
- References
- 42
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 5 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 2 · 2015: 2 · 2014: 5 · 2013: 2 · 2012: 2
- FWCI
- 14.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Electrolyte and hormonal disorders
- Keywords
- vasopressin, oxytocin, osmotic stimulation, hypertonic saline, septum, hippocampus, neurohypophysis, cerebrospinal fluid, plasma peptide levels, synaptic release, conscious rats
- MeSH terms
- Animals, Brain Chemistry, Male, Osmosis, Oxytocin, Radioimmunoassay, Rats, Inbred Strains, Vasopressins, Rats
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