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Cited by 192Neuroendocrine regulation and behaviorNeuropeptides and Animal PhysiologyOlfactory and Sensory Function Studies

Pharmacological characteristics and anatomical distribution of [3H]oxytocin-binding sites in the wistar rat brain studied by autoradiography

Freund-Mercier, M.J., Stoeckel, M.E., Palacios, J.M., Pazos, A., Reichhart, J.M., Porte, A., Richard, Ph.·1987·Neuroscience
DOI 10.1016/0306-4522(87)90113-8
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Abstract

Oxytocin-binding sites were detected by autoradiography on rat brain sections incubated in the presence of the [3H]oxytocin. These sites were characterized pharmacologically using quantitative autoradiography. High pressure liquid chromatography controls of the incubation media indicated that labelling was due to the intact [3H]oxytocin molecule. Pharmacological analysis of different locations (central amygdaloid nucleus, ventral subiculum and ventromedial hypothalamic nucleus) showed that the sites detected had a high affinity for oxytocin and also for arginine-vasopressin. In contrast, some areas known to bind vasopressin intensely, such as suprachiasmatic and lateral septum nuclei, had little or no affinity for oxytocin. Autoradiographs revealed [3H]oxytocin-binding sites in already known brain areas (olfactory centres, ventral subiculum, central amygdaloid nucleus, bed nucleus of the stria terminalis) albeit with more extensive labelling of some of these formations, in particular, the amygdaloid complex. In addition, specific [3H]oxytocin-binding sites were found in areas not yet reported to bind oxytocin, such as the paraventricular thalamic and caudate nuclei. In the hypothalamus, specific binding sites were not detected in the supraoptic and paraventricular nuclei: the only structure labelled was the ventrolateral part of the ventromedial nucleus. Discrepancies between the concentrations of [3H]oxytocin-binding sites, the known distribution of oxytocin-containing endings and electrophysiological data indicate that autoradiography, under our conditions, apparently only reveals some of the oxytocin receptors in the brain. Thus, in the hypothalamus, no relationship can be established between the known effect of oxytocin on oxytocinergic magnocellular neurons and detection of specific [3H]oxytocin-binding sites. Autoradiography may reveal mainly oxytocin-binding sites in areas receiving diverse "parasynaptic" information, where oxytocin might play a modulatory role rather than exerting rapid, short-term effects of the neurotransmitter type.

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Crossref · 10.1016/0306-4522(87)90113-8

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  • The earliest radioligand for oxytocin receptor autoradiography was tritiated oxytocin, but its cross-reactivity with vasopressin receptor 1a and long film exposure times made it unfavourable and inconvenient.

    “The earliest radioligand available for this purpose was a tritiated version of OT (14), although the cross-reactivity of this ligand with the closely-related AVPR1a, combined with long exposure times needed on film before results can be attained, caused it to be an unfavourable and generally inconvenient tool for autoradiographic analysis of the brain.”

    From Comparative Perspectives on Oxytocin and Vasopressin Receptor Research in Rodents and Primates: Translational Implications (2016) · cites this paper as [14]

  • Oxytocin receptors and corresponding messenger RNA have been localized within the VTA.

    “Oxytocin receptors and corresponding messenger RNA have been localized within the VTA (Freund-Mercier et al., 1987, Sofroniew, 1983, Vaccari et al., 1998).”

    From Oxytocin, motivation and the role of dopamine (2014) · cites this paper as Freund-Mercier et al., 1987

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Authors
Freund-Mercier, M. J., Stoeckel, M. E., Palacios, J. M., Pazos, A., Reichhart, J. M., Porte, A., & Richard, P.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Sandoz Group AG (Switzerland) (CH)
ORCID
Venue
Neuroscience
Publisher
Elsevier BV
Published
1987-02
Volume
20
Issue
2
Pages
599-614
ISSN
0306-4522 (ISSN-L)
DOI
10.1016/0306-4522(87)90113-8
OpenAlex
W1984418460
Cited by
192
References
48
Citations by year
2023: 3 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 8 · 2015: 4 · 2014: 8 · 2013: 4 · 2012: 5
FWCI
22.93
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
Keywords
oxytocin receptor, brain receptor distribution, central amygdala, ventral subiculum, ventromedial hypothalamus, bed nucleus of the stria terminalis, paraventricular thalamus, caudate nucleus, amygdala, hypothalamus
MeSH terms
Amygdala, Animals, Autoradiography, Basal Ganglia, Binding Sites, Brain, Female, Hippocampus, Hypothalamus, Lactation, Male, Oxytocin, Pregnancy, Rats, Inbred Strains, Rats

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