Versatile synthetic alternatives to Matrigel for vascular toxicity screening and stem cell expansion
Open accessAbstract
The physiological relevance of Matrigel as a cell-culture substrate and in angiogenesis assays is often called into question. Here, we describe an array-based method for the identification of synthetic hydrogels that promote the formation of robust in vitro vascular networks for the detection of putative vascular disruptors, and that support human embryonic stem cell expansion and pluripotency. We identified hydrogel substrates that promoted endothelial-network formation by primary human umbilical vein endothelial cells and by endothelial cells derived from human induced pluripotent stem cells, and used the hydrogels with endothelial networks to identify angiogenesis inhibitors. The synthetic hydrogels show superior sensitivity and reproducibility over Matrigel when evaluating known inhibitors, as well as in a blinded screen of a subset of 38 chemicals, selected according to predicted vascular disruption potential, from the Toxicity ForeCaster library of the US Environmental Protection Agency. The identified synthetic hydrogels should be suitable alternatives to Matrigel for common cell-culture applications.
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Nguyen and colleagues recently assessed more than 1200 synthetic polymer formulations for toxicity and abilities to promote implant vascularization and endothelial cell network formation.
“Nguyen and colleagues recently assessed more than 1200 synthetic polymer formulations for toxicity and abilities to promote implant vascularization and endothelial cell network formation180.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [180]
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An introduction to ROC analysis
Fawcett, T. (2006) · Pattern Recognition Letters · doi:10.1016/j.patrec.2005.10.010 · Cited by 21,879 · see citing works
Zhang, J.-H., Chung, T. D. Y., & Oldenburg, K. R. (1999) · SLAS Discovery · doi:10.1177/108705719900400206 · Cited by 6,847 · see citing works
Maynard, S. E., Min, J.-Y., Merchan, J., Lim, K.-H., Li, J., Mondal, S., Libermann, T. A., Morgan, J. P., Sellke, F. W., Stillman, I. E., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci17189 · Cited by 4,101 · see citing works
Niwa, H., Miyazaki, J., & Smith, A. G. (2000) · Nature Genetics · doi:10.1038/74199 · Cited by 3,904 · see citing works
Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells
Chambers, I., Colby, D., Robertson, M., Nichols, J., Lee, S., Tweedie, S., & Smith, A. G. (2003) · Cell · doi:10.1016/s0092-8674(03)00392-1 · Cited by 3,255 · see citing works
RGD AND OTHER RECOGNITION SEQUENCES FOR INTEGRINS
Ruoslahti, E. (1996) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.12.1.697 · Cited by 3,191 · see citing works
Vascular Channel Formation by Human Melanoma Cells in Vivo and in Vitro: Vasculogenic Mimicry
Maniotis, A. J., Folberg, R., Hess, A. R., Seftor, E. A., Gardner, L. M. G., Pe’er, J., Trent, J. M., Meltzer, P. S., & Hendrix, M. J. C. (1999) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)65173-5 · Cited by 2,049 · see citing works
Feeder-free growth of undifferentiated human embryonic stem cells
Xu, C., Inokuma, M. S., Denham, J., Golds, K., Kundu, P., Gold, J. D., & Carpenter, M. K. (2001) · Nature Biotechnology · doi:10.1038/nbt1001-971 · Cited by 1,872 · see citing works
The Balanced Accuracy and Its Posterior Distribution
Brodersen, K. H., Ong, C. S., Stephan, K. E., & Buhmann, J. M. (2010) · 2010 20th International Conference on Pattern Recognition · doi:10.1109/icpr.2010.764 · Cited by 1,816 · see citing works
Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties
Kloxin, A. M., Kasko, A. M., Salinas, C. N., & Anseth, K. S. (2009) · Science · doi:10.1126/science.1169494 · Cited by 1,748 · see citing works
Matrigel: Basement membrane matrix with biological activity
Kleinman, H. K., & Martin, G. R. (2005) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2005.05.004 · Cited by 1,673 · see citing works
Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate
Huebsch, N., Arany, P. R., Mao, A. S., Shvartsman, D. E., Ali, O. A., Bencherif, S. A., Rivera‐Feliciano, J., & Mooney, D. J. (2010) · Nature Materials · doi:10.1038/nmat2732 · Cited by 1,572 · see citing works
Matrigel: A complex protein mixture required for optimal growth of cell culture
Hughes, C. S., Postovit, L. M., & Lajoie, G. A. (2010) · PROTEOMICS · doi:10.1002/pmic.200900758 · Cited by 1,534 · see citing works
Clinical translation of angiogenesis inhibitors
Kerbel, R. S., & Folkman, J. (2002) · Nature reviews. Cancer · doi:10.1038/nrc905 · Cited by 1,513 · see citing works
Designer matrices for intestinal stem cell and organoid culture
Gjorevski, N., Sachs, N., Manfrin, A., Giger, S., Bragina, M. E., Ordóñez-Morán, P., Clevers, H., & Lutolf, M. P. (2016) · Nature · doi:10.1038/nature20168 · Cited by 1,426 · see citing works
Kubota, Y., Kleinman, H. K., Martin, G. R., & Lawley, T. J. (1988) · The Journal of Cell Biology · doi:10.1083/jcb.107.4.1589 · Cited by 1,210 · see citing works
Khetan, S., Güvendiren, M., Legant, W. R., Cohen, D. M., Chen, C. S., & Burdick, J. A. (2013) · Nature Materials · doi:10.1038/nmat3586 · Cited by 1,171 · see citing works
Folkman, J., & Haudenschild, C. C. (1980) · Nature · doi:10.1038/288551a0 · Cited by 1,044 · see citing works
Fong, T. A. T., Shawver, L. K., Sun, L., Tang, C., App, H., Powell, T., Kim, Y. H., Schreck, R., Wang, X., Risau, W., et al. (1999) · PubMed · Cited by 1,032 · see citing works
Matrix metalloproteinases and angiogenesis
Rundhaug, J. E. (2005) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2005.tb00355.x · Cited by 962 · see citing works
Materials as stem cell regulators
Murphy, W. L., McDevitt, T. C., & Engler, A. J. (2014) · Nature Materials · doi:10.1038/nmat3937 · Cited by 947 · see citing works
Self-citationWood, J. M., Bold, G., Buchdunger, E., Cozens, R. M., Ferrari, S. M., Frei, J., Hofmann, F., Mestan, J., Mett, H., O'Reilly, T., et al. (2000) · PubMed · Cited by 844 · see citing works
In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract
Arnaoutova, I., & Kleinman, H. K. (2010) · Nature Protocols · doi:10.1038/nprot.2010.6 · Cited by 760 · see citing works
ToxCast Chemical Landscape: Paving the Road to 21st Century Toxicology
Richard, A. M., Judson, R., Houck, K. A., Grulke, C., Volarath, P., Thillainadarajah, I., Yang, C., Rathman, J. F., Martin, M. T., Wambaugh, J. F., et al. (2016) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.6b00135 · Cited by 714 · see citing works
Self-citationVukicevic, S., Kleinman, H. K., Luyten, F. P., Roberts, A. B., Roche, N. S., & Reddi, A. H. (1992) · Experimental Cell Research · doi:10.1016/0014-4827(92)90397-q · Cited by 696 · see citing works
A Versatile Synthetic Extracellular Matrix Mimic via Thiol‐Norbornene Photopolymerization
Fairbanks, B. D., Schwartz, M. P., Halevi, A. E., Nuttelman, C. R., Bowman, C. N., & Anseth, K. S. (2009) · Advanced Materials · doi:10.1002/adma.200901808 · Cited by 662 · see citing works
Self-citationPolymeric Biomaterials with Degradation Sites for Proteases Involved in Cell Migration
West, J. L., & Hubbell, J. A. (1998) · Macromolecules · doi:10.1021/ma981296k · Cited by 627 · see citing works
Clark, D. E., Smith, S. K., He, Y., Day, K. A., Licence, D. R., Corps, A. N., Lammoglia, R., & Jones, D. S. (1998) · Biology of Reproduction · doi:10.1095/biolreprod59.6.1540 · Cited by 413 · see citing works
Mechanisms of Vessel Branching
Smet, F. D., Segura, I., Bock, K. D., Hohensinner, P. J., & Carmeliet, P. (2009) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.109.185165 · Cited by 398 · see citing works
Suramin: an anticancer drug with a unique mechanism of action.
Stein, C. A., Larocca, R. V., Thomas, R. V., McAtee, N., & Myers, C. E. (1989) · Journal of Clinical Oncology · doi:10.1200/jco.1989.7.4.499 · Cited by 374 · see citing works
Maynard, S. E., Min, J., Merchan, J. R., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200317189 · Cited by 365 · see citing works
Comparison of three in vitro human ‘angiogenesis’ assays with capillaries formed in vivo
Donovan, D., Brown, N. J., Bishop, E., & Lewis, C. E. (2001) · Angiogenesis · doi:10.1023/a:1012218401036 · Cited by 342 · see citing works
Human pluripotent stem cell-derived neural constructs for predicting neural toxicity
Schwartz, M. P., Hou, Z., Propson, N. E., Zhang, J., Engstrom, C. J., Costa, V. S., Jiang, P., Nguyen, B. K., Bolin, J. M., Daly, W., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1516645112 · Cited by 325 · see citing works
Self-citationHuman matrix metalloproteinase specificity studies using collagen sequence-based synthetic peptides
Nagase, H., & Fields, G. B. (1996) · Biopolymers · doi:10.1002/(sici)1097-0282(1996)40:4 · Cited by 318 · see citing works
Bioinspired Materials for Controlling Stem Cell Fate
Fisher, O. Z., Khademhosseini, A., Langer, R., & Peppas, N. A. (2009) · Accounts of Chemical Research · doi:10.1021/ar900226q · Cited by 315 · see citing works
Defined three-dimensional microenvironments boost induction of pluripotency
Caiazzo, M., Okawa, Y., Ranga, A., Piersigilli, A., Tabata, Y., & Lutolf, M. P. (2016) · Nature Materials · doi:10.1038/nmat4536 · Cited by 271 · see citing works
Controlling escape from angiogenesis inhibitors
Sennino, B., & McDonald, D. M. (2012) · Nature reviews. Cancer · doi:10.1038/nrc3366 · Cited by 265 · see citing works
Thiol/ene photocurable polymers
Morgan, C. R., Magnotta, F., & Ketley, A. D. (1977) · Journal of Polymer Science Polymer Chemistry Edition · doi:10.1002/pol.1977.170150311 · Cited by 256 · see citing works
Complementary Actions of Inhibitors of Angiopoietin-2 and VEGF on Tumor Angiogenesis and Growth
Hashizume, H., Falcón, B. L., Kuroda, T., Bałuk, P., Coxon, A., Yu, D., BREADY, J. V., Oliner, J. D., & McDonald, D. M. (2010) · Cancer Research · doi:10.1158/0008-5472.can-09-1977 · Cited by 253 · see citing works
Villa-Diaz, L. G., Ross, A. M., Lahann, J., & Krebsbach, P. H. (2012) · Stem Cells · doi:10.1002/stem.1260 · Cited by 251 · see citing works
3D niche microarrays for systems-level analyses of cell fate
Ranga, A., Gobaa, S., Okawa, Y., Mosiewicz, K., Negro, A., & Lutolf, M. P. (2014) · Nature Communications · doi:10.1038/ncomms5324 · Cited by 238 · see citing works
Neural tube morphogenesis in synthetic 3D microenvironments
Ranga, A., Girgin, M., Meinhardt, A., Eberle, D., Caiazzo, M., Tanaka, E. M., & Lutolf, M. P. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1603529113 · Cited by 221 · see citing works
Vernon, R. B., Angello, J. C., Iruela‐Arispe, M. L., Lane, T. F., & Sage, E. H. (1992) · PubMed · Cited by 218 · see citing works
Hojjat‐Farsangi, M. (2014) · International Journal of Molecular Sciences · doi:10.3390/ijms150813768 · Cited by 205 · see citing works
Profiling 976 ToxCast Chemicals across 331 Enzymatic and Receptor Signaling Assays
Sipes, N. S., Martin, M. T., Kothiya, P., Reif, D. M., Judson, R., Richard, A. M., Houck, K. A., Dix, D. J., Kavlock, R. J., & Knudsen, T. B. (2013) · Chemical Research in Toxicology · doi:10.1021/tx400021f · Cited by 203 · see citing works
Self-citationVEGF inhibition: insights from preclinical and clinical studies
Crawford, Y., & Ferrara, N. (2008) · Cell and Tissue Research · doi:10.1007/s00441-008-0675-8 · Cited by 187 · see citing works
Between molecules and morphology. Extracellular matrix and creation of vascular form.
Vernon, R. B., & Sage, E. H. (1995) · Europe PMC (PubMed Central) · Cited by 185 · see citing works
Inhibition of vessel permeability by TNP-470 and its polymer conjugate, caplostatin
Satchi‐Fainaro, R., Mamluk, R., Wang, X., Short, S. M., Nagy, J. A., Feng, D., Dvořàk, A. M., Dvorak, H. F., Puder, M., Mukhopadhyay, D., et al. (2005) · Cancer Cell · doi:10.1016/j.ccr.2005.02.007 · Cited by 173 · see citing works
Gill, B. J., Gibbons, D. L., Roudsari, L. C., Saik, J. E., Rizvi, Z. H., Roybal, J. D., Kurie, J. M., & West, J. L. (2012) · Cancer Research · doi:10.1158/0008-5472.can-12-0895 · Cited by 170 · see citing works
Musah, S., Morin, S. A., Wrighton, P. J., Zwick, D. B., Jin, S., & Kiessling, L. L. (2012) · ACS Nano · doi:10.1021/nn3039148 · Cited by 163 · see citing works
Activity profiles of 309 ToxCast™ chemicals evaluated across 292 biochemical targets
Knudsen, T. B., Houck, K. A., Sipes, N. S., Singh, A. V., Judson, R., Martin, M. T., Weissman, A. D., Kleinstreuer, N., Mortensen, H. M., Reif, D. M., et al. (2011) · Toxicology · doi:10.1016/j.tox.2010.12.010 · Cited by 135 · see citing works
Self-citationEnvironmental Impact on Vascular Development Predicted by High-Throughput Screening
Kleinstreuer, N., Judson, R., Reif, D. M., Sipes, N. S., Singh, A. V., Chandler, K. J., DeWoskin, R. S., Dix, D. J., Kavlock, R. J., & Knudsen, T. B. (2011) · Environmental Health Perspectives · doi:10.1289/ehp.1103412 · Cited by 132 · see citing works
Self-citationSynthetic hydrogels mimicking basement membrane matrices to promote cell-matrix interactions
Cruz‐Acuña, R., & Garcı́a, A. J. (2016) · Matrix Biology · doi:10.1016/j.matbio.2016.06.002 · Cited by 130 · see citing works
Enemchukwu, N. O., Cruz‐Acuña, R., Bongiorno, T., Johnson, C. T., García, J. R., Sulchek, T., & Garcı́a, A. J. (2015) · The Journal of Cell Biology · doi:10.1083/jcb.201506055 · Cited by 125 · see citing works
Zhang, R., Mjoseng, H. K., Hoeve, M. A., Bauer, N. G., Pells, S., Besseling, R., Velugotla, S., Tourniaire, G., Kishen, R. E. B., Tsenkina, Y., et al. (2013) · Nature Communications · doi:10.1038/ncomms2341 · Cited by 122 · see citing works
A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells
Francescone, R. A., III, Faibish, M., & Shao, R. (2011) · Journal of Visualized Experiments · doi:10.3791/3040 · Cited by 121 · see citing works
Belair, D. G., Whisler, J. A., Valdez, J., Velazquez, J., Molenda, J. A., Vickerman, V., Lewis, R., Daigh, C., Hansen, T. D., Mann, D. A., et al. (2015) · Stem Cell Reviews and Reports · doi:10.1007/s12015-014-9549-5 · Cited by 120 · see citing works
Self-citationDo all roads lead to Oct4? The emerging concepts of induced pluripotency
Radzisheuskaya, A., & Silva, J. (2013) · Trends in Cell Biology · doi:10.1016/j.tcb.2013.11.010 · Cited by 110 · see citing works
Beck, J. N., Singh, A., Rothenberg, A. R., Elisseeff, J. H., & Ewald, A. J. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.08.077 · Cited by 108 · see citing works
Profiling Bioactivity of the ToxCast Chemical Library Using BioMAP Primary Human Cell Systems
Houck, K., Dix, D., Judson, R., Kavlock, R., Yang, J., & Berg, E. (2009) · Journal of biomolecular screening · doi:10.1177/1087057109345525 · Cited by 101 · see citing works
Disruption of embryonic vascular development in predictive toxicology
Knudsen, T. B., & Kleinstreuer, N. (2011) · Birth Defects Research Part C Embryo Today Reviews · doi:10.1002/bdrc.20223 · Cited by 96 · see citing works
Self-citationAssembly of Human Umbilical Vein Endothelial Cells on Compliant Hydrogels
Saunders, R. L., & Hammer, D. A. (2010) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-010-0112-4 · Cited by 96 · see citing works
Optimizing the Delivery of Cancer Drugs That Block Angiogenesis
Cao, Y., & Langer, R. (2010) · Science Translational Medicine · doi:10.1126/scitranslmed.3000399 · Cited by 85 · see citing works
Raza, A., Ki, C. S., & Lin, C. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.03.086 · Cited by 81 · see citing works
Dissecting the stem cell niche with organoid models: an engineering-based approach
Murrow, L. M., Weber, R. J., & Gartner, Z. J. (2017) · Development · doi:10.1242/dev.140905 · Cited by 81 · see citing works
Nguyen, E. H., Zanotelli, M. R., Schwartz, M. P., & Murphy, W. L. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.054 · Cited by 72 · see citing works
Self-citationSchukur, L., Zorlutuna, P., Cha, J. M., Bae, H., & Khademhosseini, A. (2012) · Advanced Healthcare Materials · doi:10.1002/adhm.201200194 · Cited by 68 · see citing works
Antiangiogenesis therapy: an update after the first decade
Falco, S. D. (2014) · The Korean Journal of Internal Medicine · doi:10.3904/kjim.2014.29.1.1 · Cited by 68 · see citing works
Interleukin 2 for patients with renal cancer
Rosenberg, S. A. (2007) · Nature Clinical Practice Oncology · doi:10.1038/ncponc0926 · Cited by 67 · see citing works
Biophysical Cueing and Vascular Endothelial Cell Behavior
Wood, J. A., Liliensiek, S. J., Russell, P., Nealey, P. F., & Murphy, C. J. (2010) · Materials · doi:10.3390/ma3031620 · Cited by 53 · see citing works
El-Masri, H. A., Kleinstreuer, N., Hines, R. N., Adams, L. D., Tal, T. L., Isaacs, K., Wetmore, B. A., & Tan, Y. (2016) · Toxicological Sciences · doi:10.1093/toxsci/kfw082 · Cited by 47 · see citing works
A synthetic modular approach for modeling the role of the 3D microenvironment in tumor progression
Singh, S. P., Schwartz, M. P., Tokuda, E. Y., Luo, Y., Rogers, R. E., Fujita, M., Ahn, N. G., & Anseth, K. S. (2015) · Scientific Reports · doi:10.1038/srep17814 · Cited by 46 · see citing works
Self-citationTal, T. L., Kilty, C., Smith, A., LaLone, C. A., Kennedy, B. N., Tennant, A. H., McCollum, C. W., Bondesson, M., Knudsen, T. B., Padilla, S., et al. (2016) · Reproductive Toxicology · doi:10.1016/j.reprotox.2016.12.004 · Cited by 45 · see citing works
Self-citationLe, N. N., Zorn, S., Schmitt, S. K., Gopalan, P., & Murphy, W. L. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.09.019 · Cited by 40 · see citing works
Self-citationHansen, T. D., Koepsel, J. T., Le, N. N., Nguyen, E. H., Zorn, S., Parlato, M. B., Loveland, S. G., Schwartz, M. P., & Murphy, W. L. (2014) · Biomaterials Science · doi:10.1039/c3bm60278h · Cited by 37 · see citing works
Self-citationSunitinib but not VEGF blockade inhibits cancer stem cell endothelial differentiation
Brossa, A., Grange, C., Mancuso, L., Annaratone, L., Satolli, M. A., Mazzone, M., Camussi, G., & Bussolati, B. (2015) · Oncotarget · doi:10.18632/oncotarget.3123 · Cited by 35 · see citing works
Rashid, A., Kuppa, A., Kunwar, A., & Panda, D. (2015) · Biochemistry · doi:10.1021/bi501429j · Cited by 33 · see citing works
Human iPSC-derived endothelial cell sprouting assay in synthetic hydrogel arrays
Belair, D. G., Schwartz, M. P., Knudsen, T. B., & Murphy, W. L. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.05.020 · Cited by 31 · see citing works
Self-citationFaulkner, A., Purcell, R. H., Hibbert, A. P., Latham, S. H., Thomson, S. J. P., Hall, W. L., Wheeler-Jones, C. P. D., & Bishop‐Bailey, D. (2014) · BMC Cell Biology · doi:10.1186/s12860-014-0041-5 · Cited by 29 · see citing works
Friis, T., Engel, A., BENDIKSEN, C. D., Larsen, L. H. G., & Houen, G. (2013) · Cancers · doi:10.3390/cancers5030762 · Cited by 27 · see citing works
Specific VEGF sequestering to biomaterials: Influence of serum stability
Belair, D. G., & Murphy, W. L. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.06.033 · Cited by 26 · see citing works
Self-citationSong, J., Rolfe, B. E., Hayward, I. P., CAMPBELL, G. R., & Campbell, J. H. (2001) · Journal of Structural Biology · doi:10.1006/jsbi.2001.4327 · Cited by 20 · see citing works
sFlt-1, a Potential Antagonist for Exogenous VEGF
Belgore, F. M., Blann, A. D., & Lip, G. Y. H. (2000) · Circulation · doi:10.1161/01.cir.102.15.e108 · Cited by 18 · see citing works
Latham, A., Bruns, A., Kankanala, J., et al. (2011) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2011.01545.x · Cited by 17 · see citing works
Gharechahi, J., Pakzad, M., Mirshavaladi, S., et al. (2013) · Molecular BioSystems · doi:10.1039/c3mb70255c · Cited by 12 · see citing works
Niwano, M., Arii, S., Mori, A. S., et al. (1998) · Surgery Today · doi:10.1007/s005950050252 · Cited by 11 · see citing works
Prigozhina, N. L., Heisel, A. J., Seldeen, J. R., et al. (2013) · International Journal of Experimental Pathology · doi:10.1111/iep.12043 · Cited by 8 · see citing works
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- [49]Brodersen KH Cheng Soon O Stephan KE Buhmann JM 20th International Conference on Pattern Recognition (ICPR) 3121 3124
- [87]Nguyen EH Dataset for Versatile Synthetic Alternatives to Matrigel for Vascular Toxicity Screening and Stem Cell Expansion figshare http://dx.doi.org/10.6084/m9.figshare.c.3791386 2017 PMC5667681 29104816 · DOI
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- Authors
- Nguyen, E. H., Daly, W. T., Le, N. N. T., Farnoodian, M., Belair, D. G., Schwartz, M. P., Lebakken, C. S., Ananiev, G. E., Saghiri, M. A., Knudsen, T. B., Sheibani, N., & Murphy, W. L.
- Affiliations
- University of Wisconsin–Madison (US)
- Environmental Protection Agency (US)
- Wisconsin Department of Transportation (US)
- Corresponding author
- Murphy, William L.
- ORCID
- Venue
- Nature Biomedical Engineering
- Published
- 2017-07-11
- Volume
- 1
- Issue
- 7
- ISSN
- 2157-846X (ISSN-L)
- PMC
- PMC5667681
- OpenAlex
- W2735140255
- Cited by
- 136
- References
- 87
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- 2026: 14 · 2025: 18 · 2024: 14 · 2023: 12 · 2022: 7 · 2021: 21 · 2020: 21 · 2019: 12 · 2018: 13 · 2017: 4
- FWCI
- 5.27
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- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
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- Nature Biomedical Engineering (publishedVersion)
- PubMed (publishedVersion)
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- Funders
- U.S. Environmental Protection Agency
- U.S. Department of Agriculture
- University of Wisconsin-Madison
- National Institutes of Health (UH2TR000506)
- National Heart, Lung, and Blood Institute (T32HL007889, R01HL093282, T32HL007936)
- National Eye Institute (R01EY026078, R24EY022883, P30EY016665)
- National Cancer Institute (P30CA014520)
- National Institute of General Medical Sciences (P41GM103399, T32GM008349)
- National Institute of Environmental Health Sciences (T32ES007015)
- National Institute of Biomedical Imaging and Bioengineering (R01EB010039, R21EB016381)
- National Center for Research Resources (S10RR002781, S10RR029220, S10RR025062, P41RR002301, S10RR008438, S10RR023438)
- National Center for Advancing Translational Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- synthetic hydrogels, Matrigel alternative, angiogenesis inhibitors, human umbilical vein endothelial cells, pluripotency, cell culture substrates
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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