The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans
Open accessAbstract
During C. elegans development, the temporal pattern of many cell lineages is specified by graded activity of the heterochronic gene Lin-14. Here we demonstrate that a temporal gradient in Lin-14 protein is generated posttranscriptionally by multiple elements in the lin-14 3'UTR that are regulated by the heterochronic gene Lin-4. The lin-14 3'UTR is both necessary and sufficient to confer lin-4-mediated posttranscriptional temporal regulation. The function of the lin-14 3'UTR is conserved between C. elegans and C. briggsae. Among the conserved sequences are seven elements that are each complementary to the lin-4 RNAs. A reporter gene bearing three of these elements shows partial temporal gradient activity. These data suggest a molecular mechanism for Lin-14p temporal gradient formation: the lin-4 RNAs base pair to sites in the lin-14 3'UTR to form multiple RNA duplexes that down-regulate lin-14 translation.
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“MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression and thus act as important regulators of cellular phenotype and function.”
From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [2]
In the citing paper
“Two C. elegans miRNAs, lin-4 and let-7, control developmental timing by forming imperfect base pairing with elements within the 3Ј UTR of target mRNAs and attenuating their translation (Lee et al. 1993;Wightman et al. 1993;Olsen and Ambros 1999;Reinhart et al. 2000;Slack et al. 2000).”
From A microRNA array reveals extensive regulation of microRNAs during brain development (2003) · cites this paper as Wightman et al. 1993
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Cites
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Lee, R. C., Feinbaum, R. L., & Ambros, V. (1993) · Cell · doi:10.1016/0092-8674(93)90529-y · Cited by 12,948
Mello, C. C., Kramer, J. M., Stinchcomb, D. T., & Ambros, V. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb04966.x · Cited by 3,095
Regulating the fate of mRNA: the control of cellular iron metabolism
Klausner, R. D., Rouault, T. A., & Harford, J. B. (1993) · Cell · doi:10.1016/0092-8674(93)90046-s · Cited by 1,210
Heterochronic Mutants of the Nematode Caenorhabditis elegans
Ambros, V., & Horvitz, H. R. (1984) · Science · doi:10.1126/science.6494891 · Cited by 797
Turner, D. H., Sugimoto, N., & Freier, S. M. (1988) · Annual Review of Biophysics and Biophysical Chemistry · doi:10.1146/annurev.bb.17.060188.001123 · Cited by 647
A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans
Fire, A. Z., Harrison, S. M. W., & Dixon, D. (1990) · Gene · doi:10.1016/0378-1119(90)90224-f · Cited by 603
Sachs, A. B., & Davis, R. W. (1989) · Cell · doi:10.1016/0092-8674(89)90938-0 · Cited by 552
Mutations that lead to reiterations in the cell lineages of C. elegans.
Chalfie, M., Horvitz, H., & Sulston, J. (1981) · Cell · doi:10.1016/0092-8674(81)90501-8 · Cited by 541
RNA regulatory elements mediate control of Drosophila body pattern by the posterior morphogen nanos
Wharton, R. P., & Struhl, G. (1991) · Cell · doi:10.1016/0092-8674(91)90368-9 · Cited by 386
A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans
Ambros, V. (1989) · Cell · doi:10.1016/0092-8674(89)90171-2 · Cited by 361
The Drosophila posterior-group gene nanos functions by repressing hunchback activity
Irish, V. F., Lehmann, R., & Akam, M. E. (1989) · Nature · doi:10.1038/338646a0 · Cited by 311
Kimelman, D., & Kirschner, M. W. (1989) · Cell · doi:10.1016/0092-8674(89)90015-9 · Cited by 296
Ruvkun, G., & Giusto, J. P. (1989) · Nature · doi:10.1038/338313a0 · Cited by 234
Self-citationWightman, B., Bürglin, T. R., Gatto, J., Arasu, P., & Ruvkun, G. (1991) · Genes & Development · doi:10.1101/gad.5.10.1813 · Cited by 210
Self-citationCeMyoD accumulation defines the body wall muscle cell fate during C. elegans embryogenesis
Krause, M., Fire, A. Z., Harrison, S. M. W., Priess, J. R., & Weintraub, H. M. (1990) · Cell · doi:10.1016/0092-8674(90)90494-y · Cited by 209
Ambros, V., & Horvitz, H. R. (1987) · Genes & Development · doi:10.1101/gad.1.4.398 · Cited by 197
Edery, I., Petryshyn, R., & Sonenberg, N. (1989) · Cell · doi:10.1016/0092-8674(89)90904-5 · Cited by 196
Determination of the embryonic axes of Drosophila.
Nüsslein‐Volhard, C. (1991) · PubMed · Cited by 170
In the beginning is the end: regulation of poly(A) addition and removal during early development
Wickens, M. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90022-4 · Cited by 147
Simon, J. A., & Lis, J. T. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.7.2971 · Cited by 141
Dibb, N. J., Brown, D. M., Karn, J., Moerman, D. G., Bolten, S. L., & Waterston, R. (1985) · Journal of Molecular Biology · doi:10.1016/0022-2836(85)90170-6 · Cited by 128
bicoid mRNA localization signal: phylogenetic conservation of function and RNA secondary structure
Macdonald, P. M. (1990) · Development · doi:10.1242/dev.110.1.161 · Cited by 125
Miller, D. M., Stockdale, F. E., & Karn, J. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.8.2305 · Cited by 123
Arasu, P., Wightman, B., & Ruvkun, G. (1991) · Genes & Development · doi:10.1101/gad.5.10.1825 · Cited by 120
Self-citationSequence analysis of the complete Caenorhabditis elegans myosin heavy chain gene family
Dibb, N. J., MARUYAMA, I. N., Krause, M., & Karn, J. (1989) · Journal of Molecular Biology · doi:10.1016/0022-2836(89)90229-5 · Cited by 110
Zucker-Aprison, E., & Blumenthal, T. (1989) · Journal of Molecular Evolution · doi:10.1007/bf02602929 · Cited by 49
Developmental regulation of covalent modification of double-stranded RNA during silkmoth oogenesis
Skeiky, Y. A. W., & Iatrou, K. (1991) · Journal of Molecular Biology · doi:10.1016/0022-2836(91)90698-6 · Cited by 18
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Metadata
- Authors
- Wightman, B., Ha, I., & Ruvkun, G.
- Affiliations
- Massachusetts General Hospital (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1993-12
- Volume
- 75
- Issue
- 5
- Pages
- 855-862
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2160467221
- Cited by
- 4,292
- References
- 27
- Citations by year
- 2026: 86 · 2025: 189 · 2024: 151 · 2023: 164 · 2022: 171 · 2021: 193 · 2020: 177 · 2019: 172 · 2018: 189 · 2017: 235 · 2016: 193 · 2015: 227 · 2014: 224 · 2013: 281 · 2012: 231
- FWCI
- 6.96
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM44619, R01GM044619)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- lin-14, lin-4, post-transcriptional regulation, 3' untranslated region, heterochronic genes, RNA duplex, protein synthesis inhibition, Caenorhabditis elegans, Caenorhabditis briggsae, developmental timing, cell lineage specification
- MeSH terms
- Animals, Base Sequence, Caenorhabditis, Gene Expression Regulation, Molecular Sequence Data, Morphogenesis, RNA, Messenger, Sequence Homology, Nucleic Acid, Species Specificity, Time Factors, Protein Biosynthesis, Helminth Proteins, RNA, Antisense, Sequence Alignment, Genes, Helminth
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