Cited by 989T-cell and B-cell ImmunologyDNA Repair MechanismsGenomics and Chromatin Dynamics

The DOI matches its Crossref record; 55 of the 57 references it cites were found in a registry or an index.

Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting

Abstract

We have employed a method based on the Cre-loxP recombination system of bacteriophage P1 to generate a mouse strain in which the JH segments and the intron enhancer in the IgH locus are deleted. By analysis of immunoglobulin isotype switch recombination in heterozygous mutant B cells activated by lipopolysaccharide plus interleukin-4, we show that, on the mutant chromosome, switch recombination at the mu gene switch region is strongly suppressed, whereas the switch region of the gamma 1 gene is efficiently rearranged. These data demonstrate an independent control of switch recombination at individual switch regions and suggest that, in the process of switch recombination, the alignment of the recombining strands occurs independently of and probably after the introduction of double-strand breaks into the switch regions involved.

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Crossref · 10.1016/0092-8674(93)90644-6

Cites

55 of 55 references verified0 citation errors · 0 not checkable

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Citations

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Research works printed but not linked: 2

  1. Schüpple, 1987, Monoclonal anti-allotype antibodies towards Balbc IgM, Eur. J. Immunol., 17, 739
  2. Stavnezer, 1984, Ig H-chain switching in cultured 129 murine B lymphoma cells, commitment to an IgA or IgE switch, Curr. Topics Microbiol. Immunol., 113, 109

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Items 901–985 of 985

Metadata

Authors
Gu, H., Zou, Y.-R., & Rajewsky, K.
Affiliations
University of Cologne (DE)
Venue
Cell
Publisher
Elsevier BV
Published
1993-06
Volume
73
Issue
6
Pages
1155-1164
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2053200644
Cited by
989
References
57
Citations by year
2026: 13 · 2025: 12 · 2024: 22 · 2023: 11 · 2022: 12 · 2021: 17 · 2020: 12 · 2019: 17 · 2018: 18 · 2017: 18 · 2016: 14 · 2015: 20 · 2014: 17 · 2013: 24 · 2012: 31
FWCI
19.13
Type
journal article
Language
en
Open access
closed
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
switch regions, Cre-loxP recombination, intron enhancer, IGH locus, B cells, lipopolysaccharide, IL-4, DNA double-strand breaks
MeSH terms
Animals, Base Sequence, Female, Genes, Immunoglobulin, Genes, Switch, Male, Mice, Inbred C57BL, Mice, Mutant Strains, Molecular Sequence Data, Recombination, Genetic, Tumor Cells, Cultured, Gene Rearrangement, B-Lymphocyte, Heavy Chain, Mice

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