Essential role of the IRF8-KLF4 transcription factor cascade in murine monocyte differentiation
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Key Points: IRF8 induces the Klf4 gene in myeloid progenitors; this transcription factor cascade is essential for Ly6C+ monocyte development. IRF8 binding to genomic targets promotes H3K4me1, a chromatin signature for promoter-distal enhancers, thereby inducing gene expression.
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Pairwise expression correlation confirmed the Irf8-Klf4 regulatory relationship and suggested new regulatory interactions Irf8-Zeb2 and Gfi1-Per3.
“This confirmed an established regulatory relationship (e.g. Irf8-Klf423), and suggested new regulatory interactions (Irf8-Zeb2 and Gfi1-Per3).”
From Single-cell analysis of mixed-lineage states leading to a binary cell fate choice (2016) · cites this paper as [23]
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- Authors
- Kurotaki, D., Osato, N., Nishiyama, A., Yamamoto, M., Ban, T., Sato, H., Nakabayashi, J., Umehara, M., Miyake, N., Matsumoto, N., Nakazawa, M., Ozato, K., & Tamura, T.
- Affiliations
- Yokohama City University (JP)
- Department of Immunology,
- Department of Human Genetics, and
- National Institutes of Health (US)
- ORCID
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2013-03-07
- Volume
- 121
- Issue
- 10
- Pages
- 1839-1849
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- PMC
- PMC3591803
- OpenAlex
- W2032197035
- Cited by
- 252
- References
- 46
- Citations by year
- 2026: 13 · 2025: 19 · 2024: 15 · 2023: 14 · 2022: 19 · 2021: 20 · 2020: 20 · 2019: 21 · 2018: 16 · 2017: 31 · 2016: 25 · 2015: 21 · 2014: 15 · 2013: 2
- FWCI
- 9.41
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Blood (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (23659492, 24390246, 24790322, 24118002, 23689052, 23590343, 24249019)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Kruppel-like factors research
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Keywords
- IRF8, KLF4, monocyte differentiation, transcription factor cascade, PU.1, enhancer binding, H3K4 monomethylation, Ly6C+ monocytes, myeloid progenitor cells, chromatin binding
- MeSH terms
- Kruppel-Like Factor 4, Interferon Regulatory Factor-8, Animals, Binding Sites, Cell Differentiation, Cells, Cultured, Gene Expression Regulation, Macrophages, Mice, Inbred C57BL, Monocytes, Promoter Regions, Genetic, Transcription, Genetic, Biomarkers, Genome, Mice, Knockout, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Chromatin Immunoprecipitation, Interferon Regulatory Factors, Mice, Kruppel-Like Transcription Factors
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