Multidimensional profiling in the investigation of small-molecule-induced cell death

Wolpaw, Adam J.; Stockwell, Brent R.

doi:10.1016/b978-0-12-801430-1.00011-1

Cited by 10Cell death mechanisms and regulationComputational Drug Discovery MethodsAdvanced Proteomics Techniques and Applications

Multidimensional profiling in the investigation of small-molecule-induced cell death

Wolpaw, Adam J., Stockwell, Brent R.·2014·Methods in Enzymology

DOI 10.1016/b978-0-12-801430-1.00011-1

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Abstract

Numerous morphological variations of cell death have been described. These processes depend on a complex and overlapping cellular signaling network, making molecular definition of the pathways challenging. This review describes one solution to this problem for small-molecule-induced death, the creation of high-dimensionality profiles for compounds that can be used to define and compare pathways. Such profiles have been assembled from gene expression measurements, protein quantification, chemical-genetic interactions, chemical combination interactions, cancer cell line sensitivity profiling, quantitative imaging, and modulatory profiling. We discuss the advantages and limitations of these techniques in the study of cell death.

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Authors: Wolpaw, A. J., & Stockwell, B. R.
Affiliations: Children's Hospital of Philadelphia (US)
Howard Hughes Medical Institute (US)
ORCID: Wolpaw, Adam J.
Stockwell, Brent R.
Venue: Methods in Enzymology
Publisher: Elsevier
Published: 2014
Volume: 545
Pages: 265-302
ISSN: 0076-6879 (ISSN-L)
DOI: 10.1016/b978-0-12-801430-1.00011-1
OpenAlex: W2406815909
Cited by: 10
References: 101
Citations by year: 2025: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2016: 3 · 2015: 2
FWCI: 2.11
Type: book chapter
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
PubMed (publishedVersion)
Funders: National Cancer Institute (R01CA097061)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
Physical Sciences › Computer Science › Computational Theory and Mathematics › Computational Drug Discovery Methods
Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Keywords: cell death, gene expression profiling, protein quantification, chemical-genetic interactions, quantitative imaging, cell death pathways
MeSH terms: Humans, Signal Transduction, Cell Death, Apoptosis, Gene Expression Profiling, Small Molecule Libraries

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Multidimensional profiling in the investigation of small-molecule-induced cell death

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