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Toxicity, uptake kinetics and behavior assessment in zebrafish embryos following exposure to perfluorooctanesulphonicacid (PFOS)
Open accessAbstract
Perfluorooctanesulphonicacid (PFOS), a persistent organic contaminant, has been widely detected in the environment, wildlife and humans, but few studies have assessed its effect on aquatic organisms. The present study evaluated the effect of PFOS on zebrafish embryos. Zebrafish embryos exhibited developmental toxicity of bent spine, uninflated swim bladder, decreased heart rate and affected spontaneous movement after exposure to various PFOS concentrations (0-8mg/L) from 6 to 120h post-fertilization (hpf). The LC(50) at 120hpf was 2.20mg/L and the EC(50) at 120hpf was 1.12mg/L. Continuous exposure to PFOS from 1 to 121hpf resulted in a steady accumulation with no evidence of elimination. PFOS induced cell death at 24hpf was consistently found in the brain, eye, and tail region of embryos. PFOS exposure induced lesions in the muscle fibers with histological examination. Behavior assessment of PFOS in zebrafish embryos elevated the basal rate of swimming after 4 days of exposure, and larvae exposed to PFOS (0.25-4mg/L) for only 1h at 6dpf swam faster with increasing PFOS concentration. Embryos/larvae exposed to 8mg/L PFOS for 24h periods from 1 to 121hpf showed the highest incidence of malformations in the 97-121hpf window. This is the first study to define uptake kinetics and to focus on behavioral consequences following PFOS exposure in zebrafish. Our results further the understanding of the toxicity of PFOS to aquatic organisms and suggest the need for additional research to identify the mode of PFOS toxicity.
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“Several studies assessing PFOS exposure in larval zebrafish reported increased swimming speed or hyperactivity in PFOS-exposed adults (Huang et al., 2010; Chen et al., 2013; Gaballah et al., 2020).”
From A Critical Review and Meta-Analysis of Impacts of Per- and Polyfluorinated Substances on the Brain and Behavior (2022) · cites this paper as Huang et al., 2010
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“While studies on PFAS exposure in adult animals have not reported altered motor activity, several compounds have been shown to elicit developmental impairment of motor activity in mice, rats, and zebrafish (Johansson et al., 2008; Butenhoff et al., 2009b; Huang et al., 2010; Wang M. et al., 2011; Chen et al., 2013; Spulber et al., 2014; Hallgren et al., 2015; Goulding et al., 2017; Guo et al., 201”
From A Critical Review and Meta-Analysis of Impacts of Per- and Polyfluorinated Substances on the Brain and Behavior (2022) · cites this paper as Huang et al., 2010
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Cites
Stages of embryonic development of the zebrafish
Kimmel, C. B., Ballard, W. W., Kimmel, S. R., Ullmann, B., & Schilling, T. F. (1995) · Developmental Dynamics · doi:10.1002/aja.1002030302 · Cited by 12,037
The zebrafish book : a guide for the laboratory use of zebrafish (Danio rerio)
Westerfield, M. (1995) · Medical Entomology and Zoology · Cited by 6,813
Global Distribution of Perfluorooctane Sulfonate in Wildlife
Giesy, J. P., & Kannan, K. (2001) · Environmental Science & Technology · doi:10.1021/es001834k · Cited by 2,750
Perfluoroalkyl Acids: A Review of Monitoring and Toxicological Findings
Lau, C., Anitole, K., Hodes, C., Lai, D., Pfahles-Hutchens, A., & Seed, J. (2007) · Toxicological Sciences · doi:10.1093/toxsci/kfm128 · Cited by 2,620
Olsen, G. W., Burris, J. M., Ehresman, D. J., Froehlich, J. W., Seacat, A. M., Butenhoff, J. L., & Zobel, L. R. (2007) · Environmental Health Perspectives · doi:10.1289/ehp.10009 · Cited by 2,049
Fluorinated Surfactants and Repellents
(2001) · Textile Research Journal · doi:10.1177/004051750107100823 · Cited by 1,376
Biological Monitoring of Polyfluoroalkyl Substances: A Review
Houde, M., Martin, J. W., Letcher, R. J., Solomon, K. R., & Muir, D. C. G. (2006) · Environmental Science & Technology · doi:10.1021/es052580b · Cited by 1,280
Peer Reviewed: Perfluorochemical Surfactants in the Environment
Giesy, J. P., & Kannan, K. (2002) · Environmental Science & Technology · doi:10.1021/es022253t · Cited by 1,103
The Developmental Toxicity of Perfluoroalkyl Acids and Their Derivatives
Lau, C., Butenhoff, J. L., & Rogers, J. M. (2004) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2003.11.031 · Cited by 867
Fluorinated organics in the biosphere
Key, B. D., Howell, R. D., & Criddle, C. S. (1997) · Environmental Science & Technology · doi:10.1021/es961007c · Cited by 729
Subchronic Toxicity Studies on Perfluorooctanesulfonate Potassium Salt in Cynomolgus Monkeys
Seacat, A. M. (2002) · Toxicological Sciences · doi:10.1093/toxsci/68.1.249 · Cited by 564
Moody, C. A., Martin, J. W., Kwan, W. C., Muir, D. C. G., & Mabury, S. A. (2001) · Environmental Science & Technology · doi:10.1021/es011001+ · Cited by 560
Exposure to Perfluorooctane Sulfonate during Pregnancy in Rat and Mouse. II: Postnatal Evaluation
Lau, C., Thibodeaux, J. R., Hanson, R. G., Rogers, J. M., Grey, B. E., Stanton, M. E., Butenhoff, J. L., & Stevenson, L. A. (2003) · Toxicological Sciences · doi:10.1093/toxsci/kfg122 · Cited by 549
Quantitative Characterization of Trace Levels of PFOS and PFOA in the Tennessee River
Hansen, K. J., Johnson, H. O., Eldridge, J. S., Butenhoff, J. L., & Dick, L. A. (2002) · Environmental Science & Technology · doi:10.1021/es010780r · Cited by 546
Hypocretin/orexin overexpression induces an insomnia-like phenotype in zebrafish
Prober, D. A., Rihel, J., Onah, A. A., Sung, R.-J., & Schier, A. F. (2006) · The Journal of Neuroscience · doi:10.1523/jneurosci.4332-06.2006 · Cited by 508
Locomotion in larval zebrafish: Influence of time of day, lighting and ethanol
MacPhail, R. C., Brooks, J., Hunter, D. L., Padnos, B. K., Irons, T. D., & Padilla, S. (2008) · NeuroToxicology · doi:10.1016/j.neuro.2008.09.011 · Cited by 475
Thibodeaux, J. R., Hanson, R. G., Rogers, J. M., Grey, B. E., Barbee, B. D., Richards, J. H., Butenhoff, J. L., Stevenson, L. A., & Lau, C. (2003) · Toxicological Sciences · doi:10.1093/toxsci/kfg121 · Cited by 445
Growing Concern Over Perfluorinated Chemicals
Renner, R. (2001) · Environmental Science & Technology · doi:10.1021/es012317k · Cited by 417
Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS
Shi, X., Du, Y., Lam, P. K. S., Wu, R. S. S., & Zhou, B. (2008) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2008.01.043 · Cited by 384
Liu, C., YU, K., SHI, X., Wang, J., Lam, P. K. S., Wu, R. S. S., & Zhou, B. S. (2007) · Aquatic Toxicology · doi:10.1016/j.aquatox.2007.02.006 · Cited by 367
Neuroendocrine Effects of Perfluorooctane Sulfonate in Rats
Austin, M. E., Kasturi, B. S., Barber, M., Kannan, K., MohanKumar, P. S., & MohanKumar, S. M. J. (2003) · Environmental Health Perspectives · doi:10.1289/ehp.6128 · Cited by 347
Perfluorooctanesulfonate and Related Fluorochemicals in Human Blood Samples from China
Yeung, L. W. Y., So, M. K., Jiang, G., Taniyasu, S., Yamashita, N., Song, M., Wu, Y., Li, J., Giesy, J. P., Guruge, K. S., et al. (2005) · Environmental Science & Technology · doi:10.1021/es052067y · Cited by 344
Apoptosis in the Developing Zebrafish Embryo
Cole, L., & Ross, L. S. (2001) · Developmental Biology · doi:10.1006/dbio.2001.0432 · Cited by 315
In vivo evaluation of carbon fullerene toxicity using embryonic zebrafish
Usenko, C. Y., Harper, S. L., & Tanguay, R. L. (2007) · Carbon · doi:10.1016/j.carbon.2007.04.021 · Cited by 303
Self-citationJohansson, N., Eriksson, P., & Viberg, H. (2009) · Toxicological Sciences · doi:10.1093/toxsci/kfp029 · Cited by 292
So, M. K., Yamashita, N., Taniyasu, S., Jiang, Q., Giesy, J. P., Chen, K., & Lam, P. K. S. (2006) · Environmental Science & Technology · doi:10.1021/es060031f · Cited by 284
Suppression of Humoral Immunity in Mice following Exposure to Perfluorooctane Sulfonate
Peden‐Adams, M. M., Keller, J. M., EUDALY, J. G., Berger, J., Gilkeson, G. S., & Keil, D. E. (2008) · Toxicological Sciences · doi:10.1093/toxsci/kfn059 · Cited by 281
Toxicity of Ammonium Perfluorooctanoate in Male Cynomolgus Monkeys after Oral Dosing for 6 Months
Butenhoff, J. L. (2002) · Toxicological Sciences · doi:10.1093/toxsci/69.1.244 · Cited by 259
Behavioral indicators of sublethal toxicity in rainbow trout
Little, E. E., Archeski, R. D., Flerov, B. A., & Kozlovskaya, V. I. (1990) · Archives of Environmental Contamination and Toxicology · doi:10.1007/bf01054982 · Cited by 246
Lema, S. C., Schultz, I. R., Scholz, N. L., Incardona, J. P., & Swanson, P. (2007) · Aquatic Toxicology · doi:10.1016/j.aquatox.2007.03.002 · Cited by 235
Du, Y., Shi, X., Liu, C., Yu, K., & Zhou, B. (2008) · Chemosphere · doi:10.1016/j.chemosphere.2008.09.075 · Cited by 229
Ankley, G. T., Kuehl, D. W., Kahl, M. D., Jensen, K. M., Linnum, A. L., Leino, R. L., & Villeneuve, D. A. (2005) · Environmental Toxicology and Chemistry · doi:10.1897/04-634r.1 · Cited by 204
CELLULAR AND MOLECULAR BASIS OF CADMIUM-INDUCED DEFORMITIES IN ZEBRAFISH EMBRYOS
Cheng, S. H., Wai, A., So, C. H., & Wu, R. S. S. (2000) · Environmental Toxicology and Chemistry · doi:10.1002/etc.5620191223 · Cited by 201
Ji, K., Kim, Y., Oh, S., Ahn, B., Jo, H., & Choi, K. (2008) · Environmental Toxicology and Chemistry · doi:10.1897/07-523.1 · Cited by 191
Gestational Exposure to Perfluorooctane Sulfonate Suppresses Immune Function in B6C3F1 Mice
Keil, D. E., Mehlmann, T., Butterworth, L. F., & Peden‐Adams, M. M. (2008) · Toxicological Sciences · doi:10.1093/toxsci/kfn015 · Cited by 168
JOHNSON, J. (1984) · Fundamental and Applied Toxicology · doi:10.1016/0272-0590(84)90235-5 · Cited by 161
Perfluorooctane Sulfonate Concentrations in Surface Water in Japan
Saito, N., Sasaki, K., Nakatome, K., Harada, K. H., Yoshinaga, T., & Koizumi, A. (2003) · Archives of Environmental Contamination and Toxicology · doi:10.1007/s00244-003-0163-9 · Cited by 152
Hagenaars, A., Knapen, D., Meyer, I. J., van der Ven, K., Hoff, P. T., & Coen, W. D. (2008) · Aquatic Toxicology · doi:10.1016/j.aquatox.2008.04.002 · Cited by 150
Fishing for genes influencing vertebrate behavior: zebrafish making headway
Sison, M., Cawker, J., Buske, C., & Gerlai, R. T. (2006) · Lab Animal · doi:10.1038/laban0506-33 · Cited by 130
Ahmadi, F. A., Linseman, D. A., Grammatopoulos, T. N., Jones, S. M., Bouchard, R. J., Freed, C. R., Heidenreich, K. A., & Zawada, W. M. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.02068.x · Cited by 127
Entraining Signals Initiate Behavioral Circadian Rhythmicity in Larval Zebrafish
Hurd, M. W., & Cahill, G. M. (2002) · Journal of Biological Rhythms · doi:10.1177/074873002129002618 · Cited by 109
Rowe, C. L., Hopkins, W. A., Zehnder, C. B., & Congdon, J. D. (2001) · Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology · doi:10.1016/s1532-0456(01)00204-6 · Cited by 106
Lein, N. P. H., Fujii, S., Tanaka, S., Nozoe, M., & Tanaka, H. (2008) · Desalination · doi:10.1016/j.desal.2007.01.247 · Cited by 106
Fuentes, S., Colomina, M. T., Vicens, P., Franco‐Pons, N., & Domingo, J. L. (2007) · Toxicological Sciences · doi:10.1093/toxsci/kfm121 · Cited by 100
Behavioral effects in adult mice exposed to perfluorooctane sulfonate (PFOS)
Fuentes, S., Vicens, P., Colomina, M. T., & Domingo, J. L. (2007) · Toxicology · doi:10.1016/j.tox.2007.09.012 · Cited by 90
Hoff, P. T., Dongen, W. V., Esmans, E. L., Blust, R., & Coen, W. M. D. (2003) · Aquatic Toxicology · doi:10.1016/s0166-445x(02)00145-5 · Cited by 88
Kawamoto, K., Nishikawa, Y., Oami, K., Jin, Y., Sato, I., Saito, N., & Tsuda, S. (2008) · The Journal of Toxicological Sciences · doi:10.2131/jts.33.155 · Cited by 18
UVA-induced photo recovery during early zebrafish embryogenesis
Dong, Q., Monroe, W. T., Tiersch, T. R., & Svoboda, K. R. (2008) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2008.07.011 · Cited by 11
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Gaballah, S., Swank, A., Sobus, J. R., Howey, X. M., Schmid, J., Catron, T., McCord, J., Hines, E., Strynar, M., & Tal, T. (2020) · Environmental Health Perspectives · doi:10.1289/ehp5843 · Cited by 437
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Truong, L., Reif, D. M., Mary, L. S., Geier, M. C., Truong, H. D., & Tanguay, R. L. (2013) · Toxicological Sciences · doi:10.1093/toxsci/kft235 · Cited by 337
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Metadata
- Authors
- Huang, H., Huang, C., Wang, L., Ye, X., Bai, C., Simonich, M. T., Tanguay, R. L., & Dong, Q.
- Affiliations
- Wenzhou Medical University (CN)
- Oregon State University (US)
- Corresponding author
- Huang, Haihua
- Huang, Changjiang
- Dong, Qiaoxiang
- Venue
- Aquatic Toxicology
- Publisher
- Elsevier BV
- Published
- 2010-06
- Volume
- 98
- Issue
- 2
- Pages
- 139-147
- ISSN
- 0166-445X (ISSN-L)
- PMC
- PMC4028132
- OpenAlex
- W2066635393
- Cited by
- 283
- References
- 49
- Citations by year
- 2026: 14 · 2025: 21 · 2024: 12 · 2023: 23 · 2022: 28 · 2021: 19 · 2020: 23 · 2019: 23 · 2018: 10 · 2017: 16 · 2016: 13 · 2015: 9 · 2014: 14 · 2013: 21 · 2012: 19
- FWCI
- 15.59
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Aquatic Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Wenzhou Municipal People's Government
- National Institutes of Health
- U.S. Public Health Service (P30 00210)
- National Institute of Environmental Health Sciences (P30ES000210)
- Science and Technology Department of Zhejiang Province
- Topics
- Physical Sciences › Environmental Science › Environmental Chemistry › Per- and polyfluoroalkyl substances research
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
- Keywords
- PFOS, zebrafish embryos, developmental toxicity, uptake kinetics, persistent organic pollutants, swimming behavior, malformations, cell death, LC50, EC50, aquatic toxicity
- MeSH terms
- Animals, Behavior, Animal, Dose-Response Relationship, Drug, Embryo, Nonmammalian, Fertilization, Fluorocarbons, Heart Rate, Kinetics, Larva, Water Pollutants, Chemical, Zebrafish, Alkanesulfonic Acids
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