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miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice

Goedeke, Leigh, Rotllan, Noemi, Ramírez, Cristina M., Aranda, Juan F., Canfrán-Duque, Alberto, Araldi, Elisa, Fernández-Hernando, Ana, Langhi, Cedric, de Cabo, Rafael, Baldán, Ángel, Suárez, Yajaira, Fernández-Hernando, Carlos·2015·Atherosclerosis
DOI 10.1016/j.atherosclerosis.2015.09.033PMC PMC4975922
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  • Numerous miRNAs directly bind LDLR mRNA 3′UTR and decrease its expression, including miR-27a, miR-27b, miR-128-1, miR-128-3p, miR-130b, miR-148a, miR-184a, miR-185, and miR-301b.

    “Numerous miRNAs have been shown to directly bind LDLR mRNA 3' UTR and decrease its expression (Fig. 2), including miR-27a, miR-27b, miR-128-1, miR-128-3p, miR-130b, miR-148a, miR-184a, miR-185, and miR-301b [43][44][45][46][47][48].”

    From The low-density lipoprotein receptor: Emerging post-transcriptional regulatory mechanisms (2025) · cites this paper as [45]

    The Results (page unavailable) show that miR-27b reduces human LDLR 3′UTR reporter activity and LDLR expression. The Discussion (page unavailable) also reports that miR-27a directly regulates LDLR, but the copy does not establish the claim for the other seven listed miRNAs. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • The 3'UTR of LDLR contains one predicted miR-27b binding site at position 2472-2478 (ACUGUGA).

    “The 3’UTR of LDLR contains one predicted miR-27b binding site at position 2472-2478 (ACUGUGA)57.”

    From Pathways and Molecular Mechanisms Governing LDL Receptor Regulation (2025) · cites this paper as [57]

    The Results section states that the human LDLR 3′UTR has one conserved predicted miR-27b binding site. Our copy does not give the claimed position (2472–2478) or sequence (ACUGUGA), so those details remain unsupported. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • miR-27a, miR-27b, miR-128-1, miR-128-3p, miR-130b, miR-148a, miR-184a, miR-185, and miR-301b inhibit expression of the LDLR, decreasing LDLR.

    “miR-27a, miR-27b, miR-128-1, miR-128-3p, miR-130b, miR-148a, miR-184a, miR-185, miR-301b Inhibit expression of the LDLR ↓ [43-48]”

    From The low-density lipoprotein receptor: Emerging post-transcriptional regulatory mechanisms (2025) · cites this paper as [45]

  • C57BL/6 mice treated with an AAV expressing the precursor of miR-27b had lower hepatic LDLR protein expression.

    “Additionally, C57BL/6 mice treated with an AAV expressing the precursor of miR-27b had lower hepatic LDLR protein expression57.”

    From Pathways and Molecular Mechanisms Governing LDL Receptor Regulation (2025) · cites this paper as [57]

  • Goedeke et al. demonstrated that miR-27b regulates LDLR expression using luciferase reporter assays in Cos7 cells, where addition of miR-27b decreased luciferase activity, but mutation of the predicted binding sites reversed this effect.

    “Goedeke et. al. demonstrated that miR-27b regulates LDLR expression using luciferase reporter assays in Cos7 cells, where addition of miR-27b decreased luciferase activity, but mutation of the predicted binding sites reversed this effect57.”

    From Pathways and Molecular Mechanisms Governing LDL Receptor Regulation (2025) · cites this paper as [57]

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Citations

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Metadata

Authors
Goedeke, L., Rotllan, N., Ramírez, C. M., Aranda, J. F., Canfrán-Duque, A., Araldi, E., Fernández-Hernando, A., Langhi, C., de Cabo, R., Baldán, Á., Suárez, Y., & Fernández-Hernando, C.
Venue
Atherosclerosis
Publisher
Elsevier BV
Published
2015-12
Volume
243
Issue
2
Pages
499-509
ISSN
0021-9150
DOI
10.1016/j.atherosclerosis.2015.09.033
PMC
PMC4975922
Cited by
65
References
2
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Funders
  • National Institutes of Health (R01HL107953, R01HL107953-04S1, R01HL106063, R01HL105945, 1F31AG043318, R01HL107794)
  • American Heart Association (15SDG23000025, GRNT20460189)

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