microRNA miR-27b impairs human adipocyte differentiation and targets PPARγ
Abstract
Obesity has emerged as a global health problem with more than 1.1 billion adults to be classified as overweight or obese, and is associated with type 2 diabetes, cardiovascular disease, and several cancers. Since obesity is characterized by an increased size and/or number of adipocytes, elucidating the molecular events governing adipogenesis is of utmost importance. Recent findings indicate that microRNAs (miRNAs) - small non-protein-coding RNAs that function as post-transcriptional gene regulators - are involved in the regulatory network of adipogenesis. Whereas only a single human miRNA is known so far to be functional in adipogenesis as pro-adipogenic, several mouse miRNAs have been identified very recently as adipogenic regulators, thereby stimulating demand for studying the functional role of miRNAs during adipogenesis in human. Here, we demonstrate that miR-27b abundance decreased during adipogenesis of human multipotent adipose-derived stem (hMADS) cells. Overexpression of miR-27b blunted induction of PPARgamma and C/EBPalpha, two key regulators of adipogenesis, during early onset of adipogenesis and repressed adipogenic marker gene expression and triglyceride accumulation at late stages. PPARgamma has a predicted and highly conserved binding site in its 3'UTR and was indeed confirmed to be a direct target of miR-27b. Thus, these results suggest that the anti-adipogenic effect of miR-27b in hMADS cells is due, at least in part, to suppression of PPARgamma.
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- Authors
- Karbiener, M., Fischer, C., Nowitsch, S., Opriessnig, P., Papak, C., Ailhaud, G., Dani, C., Amri, E.-Z., & Scheideler, M.
- Affiliations
- Graz University of Technology (AT)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Scheideler, Marcel
- ORCID
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2009-12
- Volume
- 390
- Issue
- 2
- Pages
- 247-251
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2001290163
- Semantic Scholar
- 55aea5c29bb6f96cd7ee9d814257f096b8afd1e9
- Cited by
- 409
- References
- 21
- Citations by year
- 2026: 9 · 2025: 11 · 2024: 14 · 2023: 15 · 2022: 19 · 2021: 24 · 2020: 18 · 2019: 36 · 2018: 27 · 2017: 31 · 2016: 29 · 2015: 30 · 2014: 29 · 2013: 39 · 2012: 35
- FWCI
- 9.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- HAL (Le Centre pour la Communication Scientifique Directe) (submittedVersion)
- Funders
- GEN-AU programme
- Austrian Agency for International Cooperation in Education & Research
- Fondation pour le Recherche Médicale (DVO20081013470)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Keywords
- miR-27b, microRNA, adipogenesis, PPARγ, C/EBPα, adipose-derived stem cells, obesity, 3' UTR target, triglyceride accumulation, post-transcriptional regulation, anti-adipogenic effect
- MeSH terms
- Animals, Base Sequence, Gene Expression Regulation, Humans, Adipocytes, 3' Untranslated Regions, CCAAT-Enhancer-Binding Protein-alpha, MicroRNAs, PPAR gamma, Adipogenesis, Mice
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