Acetic Acid Activates Hepatic AMPK and Reduces Hyperglycemia in Diabetic KK-A (y) Mice
Abstract
Acetic acid (AcOH), which is a short-chain fatty acid, is reported to have some beneficial effects on metabolism. To test the hypothesis that feeding of AcOH exerts beneficial effects on glucose homeostasis in type 2 diabetes, we fed either a standard diet or one containing 0.3% AcOH to KK-A(y) mice for 8 weeks. Fasting plasma glucose and HbA1c levels were lower in mice fed AcOH for 8 weeks than in control mice. AcOH also reduced the expression of genes involved in gluconeogenesis and lipogenesis, which is in part regulated by 5'-AMP-activated protein kinase (AMPK) in the liver. Finally, sodium acetate, in the form of neutralized AcOH, directly activated AMPK and lowered the expression of genes such as for glucose-6-phosphatase and sterol regulatory element binding protein-1 in rat hepatocytes. These results indicate that the hypoglycemic effect of AcOH might be due to activation of AMPK in the liver.
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Clinical studies demonstrated that apple vinegar regulates gene expression via the MAPK pathway.
“Clinical studies demonstrated that apple vinegar regulates gene expression via the Mitogrn-Activated Protein Kinase (MAPK) pathway [17,18,19]”
From Fruits Vinegar: Quality Characteristics, Phytochemistry, and Functionality (2021) · cites this paper as [18]
Acetic acid acts via MAPK pathway, activated through a cascade of phosphorylation/dephosphorylation of proteins which induces inhibition of gene expression.
“These beneficial effects have been attributed to acetic acid which acts via MAPK pathway (Figure 1). It is activated through a cascade of phosphorylation/dephosphorylation of proteins which induces inhibition of gene expression [18].”
From Fruits Vinegar: Quality Characteristics, Phytochemistry, and Functionality (2021) · cites this paper as [18]
Reduction of glucose-6-phosphatase, PEPCK, and SERBP-1 decreases blood glucose levels, stimulates glucose storage, ameliorates insulin sensitivity, and decreases insulinoresistance.
“Subsequently, reduction of glucose-6-phosphatase, reduction of Phosphoenolpyruvate carboxykinase (PEPCK), and reduction of Sterol Regulatory Element-Binding Protein-1 (SERBP-1) which decrease blood glucose levels, stimulation of glucose storage, amelioration of insulin sensitivity, and decreases insulinoresistance (Figure 1) [18].”
From Fruits Vinegar: Quality Characteristics, Phytochemistry, and Functionality (2021) · cites this paper as [18]
Acetic acid is the main acid of vinegar that can ameliorate metabolic disorders and disease markers.
“The aforementioned features revealed that acetic acid is the main acid of vinegar that can ameliorate metabolic disorders and ameliorate disease markers [16,18,22,63,64,65,66,67,68,69,70,71].”
From Fruits Vinegar: Quality Characteristics, Phytochemistry, and Functionality (2021) · cites this paper as [18]
Activation of AMPK inhibits anabolic pathways through suppression of SERBP-1c.
“The activation of AMPK inhibits anabolic pathways through suppression of SERBP-1c (Figure 1) [18,19,94].”
From Fruits Vinegar: Quality Characteristics, Phytochemistry, and Functionality (2021) · cites this paper as [18]
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- Authors
- Sakakibara, S., Yamauchi, T., Oshima, Y., Tsukamoto, Y., & Kadowaki, T.
- Affiliations
- Mizkan Group (Japan) (JP)
- The University of Tokyo (JP)
- Corresponding author
- Kadowaki, Takashi
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2006-06
- Volume
- 344
- Issue
- 2
- Pages
- 597-604
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2100797163
- Cited by
- 263
- References
- 31
- Citations by year
- 2026: 10 · 2025: 19 · 2024: 27 · 2023: 14 · 2022: 13 · 2021: 31 · 2020: 29 · 2019: 15 · 2018: 16 · 2017: 8 · 2016: 13 · 2015: 13 · 2014: 9 · 2013: 13 · 2012: 6
- FWCI
- 2.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Keywords
- acetic acid, short-chain fatty acids, AMPK activation, hepatic glucose metabolism, type 2 diabetes, hyperglycemia, glucose homeostasis, gluconeogenesis, lipogenesis, glucose-6-phosphatase, SREBP1, sodium acetate, KK-Ay mice, hepatocytes
- MeSH terms
- Animals, Diabetes Mellitus, Type 2, Disease Models, Animal, Enzyme Activation, Hyperglycemia, Liver, Male, Multienzyme Complexes, Treatment Outcome, Protein Serine-Threonine Kinases, Acetic Acid, Mice, AMP-Activated Protein Kinases
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