Senescence-associated beta-galactosidase activity expression in aging hippocampal neurons
Abstract
To investigate the activity of senescence-associated beta-galactosidase (SA-beta-GAL) in the hippocampus of aging rats. Hippocampi of 6-, 18-, and 24-month-old rats were observed by histochemical staining for SA-beta-GAL and cytochemical staining for SA-beta-GAL in cultured hippocampal neurons. The activity of SA-beta-GAL doubled in hippocampal pyramidal cells of the CA3 region in rats between 6 and 18 months (14.57+/-2.74% vs. 31.66+/-14.12% SA-beta-GAL-positive, respectively), and reached 50.76+/-14.41% positive at 24 months. The activity of SA-beta-GAL also increased as a function of time upon prolonged culture of cultured hippocampal neurons with 95% of cells being SA-beta-GAL-positive at 20 days in vitro. Interestingly, no SA-beta-GAL-positive cells were found in neurons of the hippocampal dentate gyrus, a neurogenic region of the brain, at any age examined. SA-beta-GAL can be used as a senescence biomarker in determining senescent neurons in hippocampal pyramidal cells of the CA3 region in advanced aging.
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Putative senescent states have been suggested in microglia, neurons, astrocytes, and oligodendrocyte progenitor cells with aging and in various disease contexts.
“Putative senescent states have been suggested in a variety of neural subpopulations with aging and in various disease contexts, including microglia [26–30], neurons [31–33], astrocytes [26,34,35], and oligodendrocyte progenitor cells [36].”
From Untangling senescent and damage‐associated microglia in the aging and diseased brain (2023) · cites this paper as [32]
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- Authors
- Geng, Y.-Q., Guan, J.-T., Xu, X.-H., & Fu, Y.-C.
- Affiliations
- Shantou University (CN)
- Corresponding author
- Fu, Yu-Cai
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2010-06
- Volume
- 396
- Issue
- 4
- Pages
- 866-869
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2127608566
- Cited by
- 129
- References
- 16
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 8 · 2023: 9 · 2022: 10 · 2021: 17 · 2020: 13 · 2019: 13 · 2018: 9 · 2017: 6 · 2016: 7 · 2015: 6 · 2014: 5 · 2013: 5 · 2012: 3
- FWCI
- 2.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Guangdong Medical Research Foundation
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
- Keywords
- senescence-associated β-galactosidase, neuronal senescence, hippocampal aging, hippocampal pyramidal neurons, CA3 region, dentate gyrus, senescence biomarkers, brain aging
- MeSH terms
- Animals, beta-Galactosidase, Cells, Cultured, Male, Neurons, Biomarkers, Cellular Senescence, Rats, Sprague-Dawley, Pyramidal Cells, Rats, CA3 Region, Hippocampal
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