Cited by 129Telomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model OrganismsAntioxidants, Aging, Portulaca oleracea

Senescence-associated beta-galactosidase activity expression in aging hippocampal neurons

Geng, Yi-Qun, Guan, Ji-Tian, Xu, Xiao-Hu, Fu, Yu-Cai·2010·Biochemical and Biophysical Research Communications

Abstract

To investigate the activity of senescence-associated beta-galactosidase (SA-beta-GAL) in the hippocampus of aging rats. Hippocampi of 6-, 18-, and 24-month-old rats were observed by histochemical staining for SA-beta-GAL and cytochemical staining for SA-beta-GAL in cultured hippocampal neurons. The activity of SA-beta-GAL doubled in hippocampal pyramidal cells of the CA3 region in rats between 6 and 18 months (14.57+/-2.74% vs. 31.66+/-14.12% SA-beta-GAL-positive, respectively), and reached 50.76+/-14.41% positive at 24 months. The activity of SA-beta-GAL also increased as a function of time upon prolonged culture of cultured hippocampal neurons with 95% of cells being SA-beta-GAL-positive at 20 days in vitro. Interestingly, no SA-beta-GAL-positive cells were found in neurons of the hippocampal dentate gyrus, a neurogenic region of the brain, at any age examined. SA-beta-GAL can be used as a senescence biomarker in determining senescent neurons in hippocampal pyramidal cells of the CA3 region in advanced aging.

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Crossref · 10.1016/j.bbrc.2010.05.011

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  • Putative senescent states have been suggested in microglia, neurons, astrocytes, and oligodendrocyte progenitor cells with aging and in various disease contexts.

    “Putative senescent states have been suggested in a variety of neural subpopulations with aging and in various disease contexts, including microglia [26–30], neurons [31–33], astrocytes [26,34,35], and oligodendrocyte progenitor cells [36].”

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Authors
Geng, Y.-Q., Guan, J.-T., Xu, X.-H., & Fu, Y.-C.
Affiliations
Shantou University (CN)
Corresponding author
Fu, Yu-Cai
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2010-06
Volume
396
Issue
4
Pages
866-869
ISSN
0006-291X (ISSN-L)
OpenAlex
W2127608566
Cited by
129
References
16
Citations by year
2026: 5 · 2025: 8 · 2024: 8 · 2023: 9 · 2022: 10 · 2021: 17 · 2020: 13 · 2019: 13 · 2018: 9 · 2017: 6 · 2016: 7 · 2015: 6 · 2014: 5 · 2013: 5 · 2012: 3
FWCI
2.82
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
Guangdong Medical Research Foundation
Topics
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
Keywords
senescence-associated β-galactosidase, neuronal senescence, hippocampal aging, hippocampal pyramidal neurons, CA3 region, dentate gyrus, senescence biomarkers, brain aging
MeSH terms
Animals, beta-Galactosidase, Cells, Cultured, Male, Neurons, Biomarkers, Cellular Senescence, Rats, Sprague-Dawley, Pyramidal Cells, Rats, CA3 Region, Hippocampal

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