Vascularized 3D printed scaffolds for promoting bone regeneration
Open accessAbstract
3D printed scaffolds hold promising perspective for bone tissue regeneration. Inspired by process of bone development stage, 3D printed scaffolds with rapid internal vascularization ability and robust osteoinduction bioactivity will be an ideal bone substitute for clinical use. Here, we fabricated a 3D printed biodegradable scaffold that can control release deferoxamine, via surface aminolysis and layer-by-layer assembly technique, which is essential for angiogenesis and osteogenesis and match to bone development and reconstruction. Our in vitro studies show that the scaffold significantly accelerates the vascular pattern formation of human umbilical endothelial cells, boosts the mineralized matrix production, and the expression of osteogenesis-related genes during osteogenic differentiation of mesenchymal stem cells. In vivo results show that deferoxamine promotes the vascular ingrowth and enhances the bone regeneration at the defect site in a rat large bone defect model. Moreover, this 3D-printed scaffold has excellent biocompatibility that is suitable for mesenchymal stem cells grow and differentiate and possess the appropriate mechanical property that is similar to natural cancellous bone. In summary, this 3D-printed scaffold holds huge potential for clinical translation in the treatment of segmental bone defect, due to its flexibility, economical friendly and practicality.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The text we found for this paper looks like another document, so the self-check wasn’t run.
Cites
The return of a forgotten polymer—Polycaprolactone in the 21st century
Woodruff, M. A., & Hutmacher, D. W. (2010) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2010.04.002 · Cited by 4,033 · see citing works
Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone
Kusumbe, A. P., Ramasamy, S. K., & Adams, R. H. (2014) · Nature · doi:10.1038/nature13145 · Cited by 2,155 · see citing works
Osteoinduction, osteoconduction and osseointegration
Albrektsson, T. O., & Johansson, C. B. (2001) · European Spine Journal · doi:10.1007/s005860100282 · Cited by 1,756 · see citing works
Complexity in biomaterials for tissue engineering
Place, E., Evans, N. D., & Stevens, M. M. (2009) · Nature Materials · doi:10.1038/nmat2441 · Cited by 1,735 · see citing works
Recent advances in 3D printing of biomaterials
Chia, H. N., & Wu, B. M. (2015) · Journal of Biological Engineering · doi:10.1186/s13036-015-0001-4 · Cited by 1,734 · see citing works
Biomaterial developments for bone tissue engineering
Burg, K. J. L., Porter, S. E., & Kellam, J. F. (2000) · Biomaterials · doi:10.1016/s0142-9612(00)00102-2 · Cited by 1,645 · see citing works
Hutmacher, D. W., Schantz, T., Zein, I., Ng, K. W., Teoh, S. H., & Tan, K. C. (2001) · Journal of Biomedical Materials Research · doi:10.1002/1097-4636(200105)55:2 · Cited by 1,438 · see citing works
Biomaterials for bone tissue engineering
Stevens, M. M. (2008) · Materials Today · doi:10.1016/s1369-7021(08)70086-5 · Cited by 1,200 · see citing works
Osteogenesis and angiogenesis: The potential for engineering bone
Kanczler, J. M., & Oreffo, R. (2008) · European Cells and Materials · doi:10.22203/ecm.v015a08 · Cited by 984 · see citing works
Hypoxia-Inducible Factor 1 (HIF-1) Pathway
Semenza, G. L. (2007) · Science s STKE · doi:10.1126/stke.4072007cm8 · Cited by 942 · see citing works
Wang, Y., Wan, C., Deng, L., Liu, X., Cao, X., Gilbert, S. R., Bouxsein, M., Monier‐Faugere, M., Guldberg, R. E., Gerstenfeld, L. C., et al. (2007) · Journal of Clinical Investigation · doi:10.1172/jci31581 · Cited by 733 · see citing works
Self-citationBone Tissue Engineering: Hope vs Hype
Rose, F. R. A. J., & Oreffo, R. O. C. (2002) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2002.6519 · Cited by 552 · see citing works
Angiogenesis in Tissue Engineering: Breathing Life into Constructed Tissue Substitutes
Laschke, M. W., Harder, Y., Amon, M., Martín, I., Farhadi, J., Ring, A., Torio‐Padron, N., Schramm, R., Rücker, M., Junker, D., et al. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.2093 · Cited by 546 · see citing works
Activation of the hypoxia-inducible factor-1α pathway accelerates bone regeneration
Wan, C., Gilbert, S. R., Wang, Y., Cao, X., Shen, X., Ramaswamy, G., Jacobsen, K. A., Al-Aql, Z. S., Eberhardt, A. W., Gerstenfeld, L. C., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0708474105 · Cited by 517 · see citing works
Self-citationBONE MORPHOGENETIC PROTEINS IN CLINICAL APPLICATIONS
Gautschi, O. P., Frey, S. P., & Zellweger, R. (2007) · ANZ Journal of Surgery · doi:10.1111/j.1445-2197.2007.04175.x · Cited by 407 · see citing works
Engineering anatomically shaped vascularized bone grafts with hASCs and 3D-printed PCL scaffolds
Temple, J. P., Hutton, D. L., Hung, B. P., et al. (2014) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35107 · Cited by 269 · see citing works
Angiogenesis and intramembranous osteogenesis
Percival, C. J., & Richtsmeier, J. T. (2013) · Developmental Dynamics · doi:10.1002/dvdy.23992 · Cited by 266 · see citing works
Kanczler, J. M., Ginty, P. J., White, L. J., Clarke, N. M. P., Howdle, S. M., Shakesheff, K. M., & Oreffo, R. O. C. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.10.059 · Cited by 238 · see citing works
Nyberg, E. L., Rindone, A. N., Dorafshar, A. H., & Grayson, W. L. (2016) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0418 · Cited by 230 · see citing works
Komatsu, D. E., & Hadjiargyrou, M. (2004) · Bone · doi:10.1016/j.bone.2003.12.024 · Cited by 212 · see citing works
Shen, X., Wan, C., Ramaswamy, G., Mavalli, M. D., Wang, Y., Duvall, C. L., Deng, L. F., Guldberg, R. E., Eberhart, A., Clemens, T. L., et al. (2009) · Journal of Orthopaedic Research® · doi:10.1002/jor.20886 · Cited by 211 · see citing works
Self-citationChang, E. I., Loh, S. A., Ceradini, D. J., Chang, E. I., Lin, S.-E., Bastidas, N., Aarabi, S., Chan, D. A., Freedman, M. L., Giaccia, A. J., et al. (2007) · Circulation · doi:10.1161/circulationaha.107.715847 · Cited by 206 · see citing works
Domingos, M. A. N., Chiellini, E., Gloria, A., Ambrosio, L., Bártolo, P., & Chiellini, E. (2012) · Rapid Prototyping Journal · doi:10.1108/13552541211193502 · Cited by 161 · see citing works
Gómez-Lizárraga, K. K., Flores-Morales, C., Prado-Audelo, M. L. D., Álvarez-Pérez, M. A., Piña-Barba, M. C., & Escobedo, C. (2017) · Materials Science and Engineering C · doi:10.1016/j.msec.2017.05.003 · Cited by 121 · see citing works
Three-Dimensional Printing of Hollow-Struts-Packed Bioceramic Scaffolds for Bone Regeneration
Luo, Y., Zhai, D., Huan, Z., Zhu, H., Xia, L., Chang, J., & Wu, C. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b08911 · Cited by 112 · see citing works
Cheng, R., Yan, Y., Liu, H., Chen, H., Pan, G., Deng, L., & Cui, W. (2018) · Applied Materials Today · doi:10.1016/j.apmt.2018.06.008 · Cited by 112 · see citing works
Self-citationYu, W., Sun, T., Qi, C., Zhao, H., Ding, Z., Zhang, Z., Sun, B., Shen, J., Chen, F., Zhu, Y., et al. (2017) · Scientific Reports · doi:10.1038/srep44129 · Cited by 96 · see citing works
Donneys, A., Weiss, D. M., Deshpande, S. S., Ahsan, S., Tchanque-Fossuo, C. N., Sarhaddi, D., Levi, B., Goldstein, S. A., & Buchman, S. R. (2013) · Bone · doi:10.1016/j.bone.2012.10.014 · Cited by 88 · see citing works
Isolation, characterization and differentiation potential of rat bone marrow stromal cells
Vemuganti, G., Polisetti, N., Chaitanya, V., et al. (2010) · Neurology India · doi:10.4103/0028-3886.63789 · Cited by 82 · see citing works
Shao, H., Liu, A., Ke, X., et al. (2017) · Journal of Materials Chemistry B · doi:10.1039/c7tb00217c · Cited by 80 · see citing works
Najafi, R., & Sharifi, A. M. (2013) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2013.782390 · Cited by 75 · see citing works
Jia, P., Chen, H., Kang, H., Qi, J., Zhao, P., Jiang, M., Guo, L., Zhou, Q., Qian, N. D., Zhou, H., et al. (2016) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35793 · Cited by 74 · see citing works
Self-citationDaculsi, G., Fellah, B., Miramond, T., et al. (2013) · IRBM · doi:10.1016/j.irbm.2013.07.001 · Cited by 69 · see citing works
VEGF Modulates Angiogenesis and Osteogenesis in Shockwave-Promoted Fracture Healing in Rabbits
Wang, C., Huang, K., Sun, Y., et al. (2010) · Journal of Surgical Research · doi:10.1016/j.jss.2010.01.045 · Cited by 62 · see citing works
Porter, J. B. (2001) · Seminars in Hematology · doi:10.1016/s0037-1963(01)90061-7 · Cited by 62 · see citing works
Rumiński, S., Ostrowska, B., Jaroszewicz, J., et al. (2016) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2310 · Cited by 59 · see citing works
Cui, W., Li, X., Chen, J., et al. (2008) · Crystal Growth & Design · doi:10.1021/cg800641s · Cited by 58 · see citing works
Self-citationSudhakaran, S. V., & Mohanan, P. V. (2017) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2017.12.039 · Cited by 57 · see citing works
Cui, W., Li, X., Xie, C., et al. (2010) · Polymer · doi:10.1016/j.polymer.2010.03.037 · Cited by 53 · see citing works
Self-citationKang, H., Yan, Y., Jia, P., Yang, K., Guo, C., Chen, H., Jin, Q., Qian, N., Xu, X. Y., Wang, F., et al. (2016) · Cell Death and Disease · doi:10.1038/cddis.2016.339 · Cited by 50 · see citing works
Self-citationDonneys, A., Ahsan, S., Perosky, J. E., et al. (2013) · Plastic & Reconstructive Surgery · doi:10.1097/prs.0b013e3182865c57 · Cited by 48 · see citing works
Adequate hypoxia inducible factor 1α signaling is indispensable for bone regeneration
Stegen, S., Deprez, S., Eelen, G., Torrekens, S., Looveren, R. V., Goveia, J., Ghesquière, B., Carmeliet, P., & Carmeliet, G. (2016) · Bone · doi:10.1016/j.bone.2016.03.014 · Cited by 45 · see citing works
3D Printed Scaffolds as a New Perspective for Bone Tissue Regeneration: Literature Review
Mota, R. C. D. A. G., da Silva, E. O., de Lima, F. F., de Menezes, L. R., & Thiele, A. C. S. (2016) · Materials Sciences and Applications · doi:10.4236/msa.2016.78039 · Cited by 45 · see citing works
Deferoxamine Enhances Bone Regeneration in Mandibular Distraction Osteogenesis
Farberg, A. S., Sarhaddi, D., Donneys, A., et al. (2014) · Plastic & Reconstructive Surgery · doi:10.1097/01.prs.0000438050.36881.a9 · Cited by 41 · see citing works
Synergistic protection of bone vasculature and bone mass by desferrioxamine in osteoporotic mice
Wang, L., Jia, P., Shan, Y., Hao, Y., Wang, X. R., Jiang, Y., Yuan, Y., Du, Q., Zhang, H., Yang, F., et al. (2017) · Molecular Medicine Reports · doi:10.3892/mmr.2017.7451 · Cited by 40 · see citing works
Self-citationEftekhari, H., Jahandideh, A., Asghari, A., et al. (2016) · Artificial Cells Nanomedicine and Biotechnology · doi:10.1080/21691401.2016.1198360 · Cited by 37 · see citing works
Hypoxia Biomimicry to Enhance Monetite Bone Defect Repair
Drager, J., Ramírez-GarcíaLuna, J. L., Kumar, A., et al. (2017) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0526 · Cited by 35 · see citing works
Potier, E., Ferreira, E., Dennler, S., Mauviel, A., Oudina, K., Logeart‐Avramoglou, D., & Petite, H. (2008) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.92 · Cited by 34 · see citing works
Choi, J. H., Lee, Y. B., Jung, J., Hwang, S. G., Oh, I., & Kim, G. J. (2016) · Stem Cells International · doi:10.1155/2016/7932185 · Cited by 32 · see citing works
Xu, W., Ong, H., Zhu, Y. K., Liu, S., Liu, L., Zhou, K., Xu, Z.-Q., Gao, J., Zhang, Y., Ye, J., et al. (2017) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0315 · Cited by 30 · see citing works
Self-citationCastellanos, L. M. O., Marcos-Fernández, Á. A., & Martínez‐Richa, A. (2009) · Polymers for Advanced Technologies · doi:10.1002/pat.1531 · Cited by 28 · see citing works
Effects of deferoxamine on the osteogenic differentiation of human periodontal ligament cells
Mu, S., Guo, S., Wang, X., Zhan, Y., Li, Y., Jiang, Y., Zhang, R., & Zhang, B. (2017) · Molecular Medicine Reports · doi:10.3892/mmr.2017.7810 · Cited by 25 · see citing works
Donneys, A., Nelson, N. S., Perosky, J. E., Polyatskaya, Y., Rodriguez, J. J., Figueredo, C., Vasseli, C. A., Ratliff, H. C., Deshpande, S. S., Kozloff, K. M., et al. (2015) · Bone · doi:10.1016/j.bone.2015.12.051 · Cited by 23 · see citing works
Bone-Healing Capacity of PCL/PLGA/Duck Beak Scaffold in Critical Bone Defects in a Rabbit Model
Lee, J. Y., Son, S. J., Son, J. S., et al. (2016) · BioMed Research International · doi:10.1155/2016/2136215 · Cited by 23 · see citing works
Donneys, A., Nelson, N. S., Page, E. E., et al. (2014) · Head & Neck · doi:10.1002/hed.23744 · Cited by 19 · see citing works
XU, G., Xue, M., Wang, H., & Chun, X. (2015) · Experimental and Therapeutic Medicine · doi:10.3892/etm.2015.2319 · Cited by 11 · see citing works
Bringing regenerating tissues to life: the importance of angiogenesis in tissue engineering
Carlier, A., van Gastel, N., Geris, L., et al. (2014) · Open Repository and Bibliography (University of Liège) · Cited by 5 · see citing works
Donneys, A., Ahsan, S., Nelson, N. S., & Buchman, S. R. (2013) · Plastic & Reconstructive Surgery · doi:10.1097/prs.0b013e3182a9806a · Cited by 1 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Synthesis and Biological Application of Polylactic Acid
Li, G., Zhao, M., Xu, F., Yang, B., Li, X., Meng, X., Teng, L., Sun, F., & Li, Y. (2020) · Molecules · doi:10.3390/molecules25215023 · Cited by 492 · see citing works
Fabrication of Biomedical Scaffolds Using Biodegradable Polymers.
Kirillova, A., Yeazel, T. R., Asheghali, D., Petersen, S. R., Dort, S., Gall, K., & Becker, M. L. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01200 · Cited by 368 · see citing works
Liu, Y., Zhu, Z., Pei, X., Zhang, X., Cheng, X., Hu, S., Gao, X., Wang, J., Chen, J., & Wan, Q. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.0c12090 · Cited by 259 · see citing works
Zha, Y., Li, Y., Lin, T., Chen, J., Zhang, S., & Wang, J. (2021) · Theranostics · doi:10.7150/thno.50741 · Cited by 232 · see citing works
Wu, M., Liu, H., Zhu, Y., Wu, P., Chen, Y., Deng, Z., Zhu, X., & Cai, L. (2024) · Theranostics · doi:10.7150/thno.97335 · Cited by 189 · see citing works
Design, printing, and engineering of regenerative biomaterials for personalized bone healthcare
Jia, Z., Xu, X., Zhu, D., & Zheng, Y. (2023) · Progress in Materials Science · doi:10.1016/j.pmatsci.2023.101072 · Cited by 176 · see citing works
Zhuang, Z., Zhang, Y., Sun, S., Li, Q., Chen, K., An, C., Wang, L., van den Beucken, J. J. J. P., & Wang, H. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c00295 · Cited by 126 · see citing works
Self-citationWu, M., Chen, F., Liu, H., Wu, P., Yang, Z., Zhang, Z., Su, J., Cai, L., & Zhang, Y. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100458 · Cited by 55 · see citing works
Self-citationβ-Ecdysterone Enhanced Bone Regeneration Through the BMP-2/SMAD/RUNX2/Osterix Signaling Pathway
Yan, C.-P., Wang, X.-K., Jiang, K., Yin, C., Xiang, C., Wang, Y., Pu, C., Chen, L., & Li, Y.-L. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.883228 · Cited by 49 · see citing works
Biomimetic hepatic lobules from three-dimensional imprinted cell sheets
Wang, J., Huang, D., Chen, H., & Zhao, Y. (2024) · Science Bulletin · doi:10.1016/j.scib.2024.02.030 · Cited by 37 · see citing works
Self-citation
- Authors
- Yan, Y., Chen, H., Zhang, H., Guo, C., Yang, K., Chen, K., Cheng, R., Qian, N., Sandler, N., Zhang, Y. S., Shen, H., Qi, J., Cui, W., & Deng, L.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Åbo Akademi University (FI)
- Brigham and Women's Hospital (US)
- Corresponding author
- Cui, Wenguo
- Deng, Lianfu
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2019-01
- Volume
- 190-191
- Pages
- 97-110
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2899366145
- Cited by
- 475
- References
- 58
- Citations by year
- 2026: 28 · 2025: 53 · 2024: 55 · 2023: 92 · 2022: 101 · 2021: 71 · 2020: 54 · 2019: 21
- FWCI
- 14.56
- Type
- journal article
- Language
- en
- Open access
- green
- research.abo.fi
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Åbo Akademi University Research Portal (publishedVersion, other-oa)
- Funders
- National Natural Science Foundation of China (81572099, 81702125)
- Shanghai Municipal Commission of Health and Family Planning (201440411)
- Shanghai Sailing Program (17YF1411300)
- Shanghai Collaborative Innovation Center for Translational Medicine (TM201803)
- Shanghai Jiading Science and Technology Commission (JDKW-2017-W10)
- Shanghai Municipal Education Commission—Gaofeng Clinical Medicine (20171906, 17XJ11004, 201840027)
- Science and Technology Commission of Shanghai Municipality (18ZR1434200)
- Shanghai municipal health and Family Planning Commission
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Anatomy and Medical Technology
- Keywords
- 3D-printed scaffolds, bone regeneration, vascularization, deferoxamine, controlled drug release, aminolysis, layer-by-layer assembly, angiogenesis, osteogenic differentiation, mesenchymal stem cells, bone defect repair, biodegradable scaffolds, biocompatibility, mechanical properties
- MeSH terms
- Animals, Biocompatible Materials, Bone Regeneration, Cells, Cultured, Deferoxamine, Delayed-Action Preparations, Humans, Male, Osteogenesis, Rats, Sprague-Dawley, Neovascularization, Physiologic, Tissue Scaffolds, Mesenchymal Stem Cells, Human Umbilical Vein Endothelial Cells, Printing, Three-Dimensional
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
