Advances in techniques for assessment of microalgal lipids
Open accessAbstract
Microalgae are a varied group of organisms with considerable commercial potential as sources of various biochemicals, storage molecules and metabolites such as lipids, sugars, amino acids, pigments and toxins. Algal lipids can be processed to bio-oils and biodiesel. The conventional method to estimate algal lipids is based on extraction using solvents and quantification by gravimetry or chromatography. Such methods are time consuming, use hazardous chemicals and are labor intensive. For rapid screening of prospective algae or for management decisions (e.g. decision on timing of harvest), a rapid, high throughput, reliable, accurate, cost effective and preferably nondestructive analytical technique is desirable. This manuscript reviews the application of fluorescent lipid soluble dyes (Nile Red and BODIPY 505/515), nuclear magnetic resonance (NMR), Raman, Fourier transform infrared (FTIR) and near infrared (NIR) spectroscopy for the assessment of lipids in microalgae.
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NIR has been used extensively as a turbidimetric optical density estimation tool in microalgae productions and has also been used for density control to optimize productions.
“Unlike other vibrational spectroscopies, NIR has been used extensively as a turbidimetric optical density estimation tool in microalgae productions and has also been used for density control to optimize productions [69].”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [69]
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- Authors
- Challagulla, V., Nayar, S., Walsh, K., & Fabbro, L.
- Affiliations
- Central Queensland University (AU)
- South Australian Research and Development Institute (AU)
- Corresponding author
- Challagulla, Vineela
- Venue
- Critical Reviews in Biotechnology
- Publisher
- Informa UK Limited
- Published
- 2016-07-15
- Volume
- 37
- Issue
- 5
- Pages
- 566-578
- ISSN
- 0738-8551 (ISSN-L)
- 1549-7801
- OpenAlex
- W2464799723
- Cited by
- 27
- References
- 112
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 5 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 1 · 2017: 1
- FWCI
- 0.45
- Type
- journal article
- Language
- en
- Open access
- green
- figshare.com
- Licence
- cc-by
- Locations
- Critical Reviews in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Acquire (CQUniversity) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- microalgal lipids, lipid quantification, Nile Red, biodiesel feedstock, NMR spectroscopy, Raman spectroscopy, FTIR spectroscopy, near-infrared spectroscopy, high-throughput screening
- MeSH terms
- Lipids, Prospective Studies, Biofuels, Microalgae
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