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aPKC Phosphorylation of Bazooka Defines the Apical/Lateral Border in Drosophila Epithelial Cells

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Morais-de-Sá, Eurico, Mirouse, Vincent, St Johnston, Daniel·2010·Cell
DOI 10.1016/j.cell.2010.02.040
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Abstract

Bazooka (PAR-3), PAR-6, and aPKC form a complex that plays a key role in the polarization of many cell types. In epithelial cells, however, Bazooka localizes below PAR-6 and aPKC at the apical/lateral junction. Here, we show that Baz is excluded from the apical aPKC domain in epithelia by aPKC phosphorylation, which disrupts the Baz/aPKC interaction. Removal of Baz from the complex is epithelial-specific because it also requires the Crumbs complex, which prevents the Baz/PAR-6 interaction. In the absence of Crumbs or aPKC phosphorylation of Baz, mislocalized Baz recruits adherens junction components apically, leading to a loss of the apical domain and an expansion of lateral. Thus, apical exclusion of Baz by Crumbs and aPKC defines the apical/lateral border. Although Baz acts as an aPKC targeting and specificity factor in nonepithelial cells, our results reveal that it performs a complementary function in positioning the adherens junction in epithelia.

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Metadata

Authors
Morais-de-Sá, E., Mirouse, V., & St Johnston, D.
Venue
Cell
Publisher
Elsevier BV
Published
2010-04
Volume
141
Issue
3
Pages
509-523
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2010.02.040
OpenAlex
W1990188178
Cited by
282
References
7
FWCI
10.87
Type
journal article
Language
en
Open access
hybrid
Licence

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CoChat. (2026). Citation check: aPKC Phosphorylation of Bazooka Defines the Apical/Lateral Border in Drosophila Epithelial Cells. https://app.cochat.ai/papers/10.1016/j.cell.2010.02.040
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Morais-de-Sá, E., Mirouse, V., & St Johnston, D. (2010). aPKC Phosphorylation of Bazooka Defines the Apical/Lateral Border in Drosophila Epithelial Cells. Cell. https://doi.org/10.1016/j.cell.2010.02.040

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