Crystal structure of Cas9 in complex with guide RNA and target DNA

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Items 101–200 of 1,002

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3. Increasing the specificity of CRISPR systems with engineered RNA secondary structures
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4. Development of an intein-mediated split-Cas9 system for gene therapy
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6. Structure and Engineering of Francisella novicida Cas9
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7. Target specificity of the CRISPR‐Cas9 system
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8. Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in <i>Saccharomyces cerevisiae</i>
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9. Dynamics of CRISPR-Cas9 genome interrogation in living cells
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10. Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editing
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11. Disabling Cas9 by an anti-CRISPR DNA mimic
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12. Kinetic Basis for DNA Target Specificity of CRISPR-Cas12a
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13. Evolutionary Genomics of Defense Systems in Archaea and Bacteria
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14. Methodologies for Improving HDR Efficiency
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15. Molecular Mechanisms of RNA Targeting by Cas13-containing Type VI CRISPR–Cas Systems
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16. Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases
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17. Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes
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18. Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities
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19. Origins of Programmable Nucleases for Genome Engineering
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20. CRISPR-Cas9 system: A new-fangled dawn in gene editing
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21. CRISPR-Cas9-based Photoactivatable Transcription System
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22. Cpf1 Is a Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria
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23. A decade of discovery: CRISPR functions and applications
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24. Phage-assisted evolution and protein engineering yield compact, efficient prime editors
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26. Towards CRISPR /Cas crops – bringing together genomics and genome editing
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27. DSSR: an integrated software tool for dissecting the spatial structure of RNA
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28. Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila
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29. Modification of the PthA4 effector binding elements in Type I Cs LOB 1 promoter using Cas9/sg RNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCs LOB 1.3 infection
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30. Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1
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31. A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering
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32. Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity
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33. CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)
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34. CRISPR Ethics: Moral Considerations for Applications of a Powerful Tool
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35. Gaussian accelerated molecular dynamics: Principles and applications
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36. Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d
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37. Refined sgRNA efficacy prediction improves large- and small-scale CRISPR–Cas9 applications
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39. Rational design of a split-Cas9 enzyme complex
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40. CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity
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41. Integrating CRISPR-Cas12a with a DNA circuit as a generic sensing platform for amplified detection of microRNA
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42. Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity
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43. CRISPR Crops: Plant Genome Editing Toward Disease Resistance
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44. PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures
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45. PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease
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46. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus
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47. Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease
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48. Structural basis for mismatch surveillance by CRISPR–Cas9
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49. Optical Control of CRISPR/Cas9 Gene Editing
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50. CRISPR/cas systems redefine nucleic acid detection: Principles and methods
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51. Genome modification by CRISPR /Cas9
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52. Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing
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53. Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
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54. Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality
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55. Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein
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56. Elimination of Toxic Microsatellite Repeat Expansion RNA by RNA-Targeting Cas9
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57. Cas9-mediated targeting of viral RNA in eukaryotic cells
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58. Partial DNA-guided Cas9 enables genome editing with reduced off-target activity
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59. Genome Editing with Engineered Nucleases in Plants
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60. Crystal structure of the CRISPR RNA–guided surveillance complex from Escherichia coli
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62. The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells
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63. Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression
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64. Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis
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65. dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression
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75. Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing
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77. A CRISPR view of development
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81. Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria
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85. Cas9 function and host genome sampling in Type II-A CRISPR–Cas adaptation
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86. CRISPR-based genome editing through the lens of DNA repair
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