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Chain elongation in anaerobic reactor microbiomes to recover resources from waste
Open accessAbstract
Different microbial pathways can elongate the carbon chains of molecules in open cultures of microbial populations (i.e. reactor microbiomes) under anaerobic conditions. Here, we discuss three such pathways: 1. homoacetogenesis to combine two carbon dioxide molecules into acetate; 2. succinate formation to elongate glycerol with one carbon from carbon dioxide; and 3. reverse β oxidation to elongate short-chain carboxylates with two carbons into medium-chain carboxylates, leading to more energy-dense and insoluble products (e.g. easier to separate from solution). The ability to use reactor microbiomes to treat complex substrates can simultaneously address two pressing issues: 1. providing proper waste management; and 2. producing renewable chemicals and fuels.
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Cites
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Weiland, P. (2009) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-009-2246-7 · Cited by 2,824
Microbial electrosynthesis — revisiting the electrical route for microbial production
Rabaey, K., & Rozendal, R. A. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2422 · Cited by 1,676
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Agler, M. T., Wrenn, B. A., Zinder, S. H., & Angenent, L. T. (2010) · Trends in biotechnology · doi:10.1016/j.tibtech.2010.11.006 · Cited by 821
Self-citationAlternative Pathways of Carbon Dioxide Fixation: Insights into the Early Evolution of Life?
Fuchs, G. (2011) · Annual Review of Microbiology · doi:10.1146/annurev-micro-090110-102801 · Cited by 726
The genome of Clostridium kluyveri , a strict anaerobe with unique metabolic features
Seedorf, H., Fricke, W. F., Veith, B., Brüggemann, H., Liesegang, H., Strittmatter, A., Miethke, M., Buckel, W., Hinderberger, J., Li, F., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0711093105 · Cited by 506
Bacterial community structures are unique and resilient in full-scale bioenergy systems
Werner, J. J., Knights, D., García, M. L., Scalfone, N. B., Smith, S., Yarasheski, K. E., Cummings, T. A., Beers, A. R., Knight, R. D., & Angenent, L. T. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1015676108 · Cited by 493
Self-citationSteinbusch, K. J. J., Hamelers, H. V. M., Plugge, C. M., & Buisman, C. J. N. (2011) · Energy Environ. Sci. · doi:10.1039/c0ee00282h · Cited by 453
Electrosynthesis of Commodity Chemicals by an Autotrophic Microbial Community
Marshall, C. W., Ross, D. E., Fichot, E. B., Norman, R. S., & May, H. D. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.02401-12 · Cited by 423
Tracy, B. P., Jones, S. W., Fast, A. G., Indurthi, D. C., & Papoutsakis, E. T. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.10.008 · Cited by 408
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Agler, M. T., Spirito, C. M., Usack, J. G., Werner, J. J., & Angenent, L. T. (2012) · Energy & Environmental Science · doi:10.1039/c2ee22101b · Cited by 376
Self-citationMarshall, C. W., Ross, D. E., Fichot, E. B., Norman, R. S., & May, H. D. (2013) · Environmental Science & Technology · doi:10.1021/es400341b · Cited by 356
Pathway engineering and synthetic biology using acetogens
Schiel‐Bengelsdorf, B., & Dürre, P. (2012) · FEBS Letters · doi:10.1016/j.febslet.2012.04.043 · Cited by 265
Cukalovic, A., & Stevens, C. V. (2008) · Biofuels Bioproducts and Biorefining · doi:10.1002/bbb.105 · Cited by 246
Separation and Purification Technologies in Biorefineries
(2013) · Wiley eBooks · doi:10.1002/9781118493441 · Cited by 241
A Generalized Method for Thermodynamic State Analysis of Environmental Systems
Kleerebezem, R., & van Loosdrecht, M. C. M. (2010) · Critical Reviews in Environmental Science and Technology · doi:10.1080/10643380802000974 · Cited by 225
Metabolic and practical considerations on microbial electrosynthesis
Rabaey, K., Girguis, P. R., & Nielsen, L. K. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.01.010 · Cited by 223
Self-citationZhang, F., Ding, J., Zhang, Y., Chen, M., Ding, Z., van Loosdrecht, M. C. M., & Zeng, R. J. (2013) · Water Research · doi:10.1016/j.watres.2013.07.033 · Cited by 207
Bioelectrochemical Production of Caproate and Caprylate from Acetate by Mixed Cultures
Eerten-Jansen, M. C. A. A. V., ter Heijne, A., Grootscholten, T. I. M., Steinbusch, K. J. J., Sleutels, T. H. J. A., Hamelers, H. V. M., & Buisman, C. J. N. (2013) · ACS Sustainable Chemistry & Engineering · doi:10.1021/sc300168z · Cited by 201
Latif, H., Zeidan, A. A., Nielsen, A. T., & Zengler, K. (2014) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.12.001 · Cited by 199
Steinbusch, K. J. J., Hamelers, H. V. M., & Buisman, C. J. N. (2008) · Water Research · doi:10.1016/j.watres.2008.05.032 · Cited by 190
Lactate and Acrylate Metabolism by Megasphaera elsdenii under Batch and Steady-State Conditions
Prabhu, R., Altman, E., & Eiteman, M. A. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.02443-12 · Cited by 186
Upgrading dilute ethanol from syngas fermentation to n-caproate with reactor microbiomes
Vasudevan, D., Richter, H., & Angenent, L. T. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2013.09.105 · Cited by 174
Self-citationEnabling Unbalanced Fermentations by Using Engineered Electrode-Interfaced Bacteria
Flynn, J. M., Ross, D. E., Hunt, K. A., Bond, D. R., & Gralnick, J. A. (2010) · mBio · doi:10.1128/mbio.00190-10 · Cited by 174
Muñoz‐Tamayo, R., Laroche, B., Walter, É., Dore, J. M., Duncan, S. H., Flint, H. J., & Leclerc, M. (2011) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2011.01085.x · Cited by 174
Chain elongation of acetate and ethanol in an upflow anaerobic filter for high rate MCFA production
Grootscholten, T. I. M., Steinbusch, K. J. J., Hamelers, H. V. M., & Buisman, C. J. N. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.10.165 · Cited by 171
A Two-Stage Continuous Fermentation System for Conversion of Syngas into Ethanol
Richter, H., Martin, M. E., & Angenent, L. T. (2013) · Energies · doi:10.3390/en6083987 · Cited by 170
Self-citationGrootscholten, T. I. M., Steinbusch, K. J. J., Hamelers, H. V. M., & Buisman, C. J. N. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.02.114 · Cited by 157
Fatty acid alkyl esters: perspectives for production of alternative biofuels
Röttig, A., Wenning, L., Bröker, D., & Steinbüchel, A. (2009) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-009-2383-z · Cited by 144
Munasinghe, P. C., & Khanal, S. K. (2010) · Biotechnology Progress · doi:10.1002/btpr.473 · Cited by 142
Kim, S. A., & Rhee, M. S. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.02164-13 · Cited by 137
Linking thermodynamics and kinetics to assess pathway reversibility in anaerobic bioprocesses
González‐Cabaleiro, R., Lema, J. M., Rodríguez, J., & Kleerebezem, R. (2013) · Energy & Environmental Science · doi:10.1039/c3ee42754d · Cited by 136
Sharma, M., Aryal, N., Sarma, P. M., Vanbroekhoven, K., Lal, B., Benetton, X. D., & Pant, D. (2013) · Chemical Communications · doi:10.1039/c3cc42570c · Cited by 131
1-Hexene: a renewable C6 platform for full-performance jet and diesel fuels
Harvey, B. G., & Meylemans, H. A. (2013) · Green Chemistry · doi:10.1039/c3gc41554f · Cited by 130
Glycerol fermentation by (open) mixed cultures: A chemostat study
Temudo, M. F., Poldermans, R., Kleerebezem, R., & van Loosdrecht, M. C. M. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.21857 · Cited by 129
Chowdhury, N. P., Mowafy, A. M., Demmer, J. K., Upadhyay, V., Koelzer, S., Jayamani, E., Kahnt, J., Hornung, M., Demmer, U., Ermler, U., et al. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.521013 · Cited by 128
McKinlay, J. B., Laivenieks, M., Schindler, B. D., McKinlay, A., Siddaramappa, S., Challacombe, J. F., Lowry, S. R., Clum, A., Lapidus, A. L., Burkhart, K. B., et al. (2010) · BMC Genomics · doi:10.1186/1471-2164-11-680 · Cited by 126
Perez, J. M., Richter, H., Loftus, S. E., & Angenent, L. T. (2013) · Biotechnology and Bioengineering · doi:10.1002/bit.24786 · Cited by 126
Self-citationReaction engineering analysis of hydrogenotrophic production of acetic acid by Acetobacterium woodii
Demler, M., & Weuster‐Botz, D. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22935 · Cited by 126
Production of fuels and chemicals from waste by microbiomes
Marshall, C. W., LaBelle, E. V., & May, H. D. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.03.016 · Cited by 120
Weimer, P. J., & Moen, G. N. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4645-4 · Cited by 119
Zaks, D. P. M., Winchester, N., Kucharik, C. J., Barford, C. C., Paltsev, S., & Reilly, J. M. (2011) · Environmental Science & Technology · doi:10.1021/es104227y · Cited by 105
Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products
Li, C., Lesnik, K. L., & Liu, H. (2013) · Energies · doi:10.3390/en6094739 · Cited by 101
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Agler, M. T., Spirito, C. M., Usack, J. G., Werner, J. J., & Angenent, L. T. (2014) · Water Science and Technology · doi:10.2166/wst.2013.549 · Cited by 92 · cited 4×
“Extraction Operating conditions with lower pH values result in toxic environments when short-chain or medium-chain carboxylates are being generated [33], and therefore a need was identified to continuously extract the product from the fermentation broth [24].”
BackgroundMethodInfluentialSelf-citationZhang, X., Li, C., Wang, Y., Luo, J., & Xu, T. (2011) · Journal of Membrane Science · doi:10.1016/j.memsci.2011.05.059 · Cited by 91
Zou, W., Zhu, L., Li, H., & Ya-jie Tang, A. (2011) · Microbial Cell Factories · doi:10.1186/1475-2859-10-87 · Cited by 76
Dennis, P. G., Harnisch, F., Yeoh, Y. K., Tyson, G. W., & Rabaey, K. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.00569-13 · Cited by 75
Self-citationNi, B., Liu, H., Nie, Y., Zeng, R. J., Du, G., Chen, J., & Yu, H. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22908 · Cited by 65
Impact of dissolved hydrogen partial pressure on mixed culture fermentations
de Kok, S., Meijer, J., van Loosdrecht, M. C. M., & Kleerebezem, R. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4400-x · Cited by 56
Huang, H., & Ramaswamy, S. (2013) · doi:10.1002/9781118493441.ch1 · Cited by 56
Enhanced succinic acid production by Actinobacillus succinogenes after genome shuffling
Zheng, P., Zhang, K., Yan, Q., Xu, Y., & Sun, Z. (2013) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-013-1283-5 · Cited by 52
Forrest, A. K., Hernández, J. E., & Holtzapple, M. T. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.04.049 · Cited by 46
Inactivation of Ascaris suum by Short-Chain Fatty Acids
Butkus, M., Hughes, K. T., Bowman, D. D., Liotta, J. L., Jenkins, M. B., & Labare, M. P. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.01675-10 · Cited by 35
Microbial electrocatalysis to guide biofuel and biochemical bioprocessing
Angenent, L. T., & Rosenbaum, M. A. (2013) · Biofuels · doi:10.4155/bfs.12.93 · Cited by 25
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Metadata
- Authors
- Spirito, C. M., Richter, H., Rabaey, K., Stams, A. J., & Angenent, L. T.
- Affiliations
- Cornell University (US)
- Ghent University (BE)
- Wageningen University & Research (NL)
- Corresponding author
- Angenent, Largus T
- Venue
- Current Opinion in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2014-06
- Volume
- 27
- Pages
- 115-122
- ISSN
- 0958-1669 (ISSN-L)
- OpenAlex
- W2111185205
- Cited by
- 479
- References
- 55
- Citations by year
- 2026: 56 · 2025: 48 · 2024: 56 · 2023: 46 · 2022: 46 · 2021: 59 · 2020: 43 · 2019: 34 · 2018: 24 · 2017: 31 · 2016: 19 · 2015: 15 · 2014: 1
- FWCI
- 4.04
- Type
- journal article
- Language
- en
- Open access
- green
- research.wur.nl
- Locations
- Current Opinion in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- U. S. Army Research Office (W911NF-12-1-0555)
- Cornell University Agricultural Experiment Station (NYC-123452)
- National Institutes for Food and Agriculture (NIFA)
- U.S. Department of Agriculture
- European Research Council Starter Grant Electrotalk
- the Multidisciplinary Research Partnership Ghent Bio-Economy
- Netherlands Science Foundation (CW-TOP 700.55.343)
- European Research Council (323009)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Anaerobic Digestion and Biogas Production
- Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- chain extension, homoacetogenesis, reverse β-oxidation, CO2 utilization, glycerol, waste management, renewable chemicals, renewable fuels, microbial metabolism
- MeSH terms
- Acetates, Anaerobiosis, Carbon Dioxide, Carboxylic Acids, Electrodes, Oxidation-Reduction, Waste Management, Bioreactors, Succinic Acid, Recycling, Microbiota
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