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An unbiased template of the Drosophila brain and ventral nerve cord

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Bogovic, John A., Otsuna, Hideo, Heinrich, Larissa, Ito, Masayoshi, Jeter, Jennifer, Meissner, Geoffrey, Nern, Aljoscha, Colonell, Jennifer +3 more·2020·PLOS ONE
DOI 10.1371/journal.pone.0236495PMC PMC7774840

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Abstract

The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individual neurons and neural subtypes. Brain templates are essential for comparative biological studies because they enable analyzing many individuals in a common reference space. Several central brain templates exist for Drosophila , but every one is either biased, uses sub-optimal tissue preparation, is imaged at low resolution, or does not account for artifacts. No publicly available Drosophila ventral nerve cord template currently exists. In this work, we created high-resolution templates of the Drosophila brain and ventral nerve cord using the best-available technologies for imaging, artifact correction, stitching, and template construction using groupwise registration. We evaluated our central brain template against the four most competitive, publicly available brain templates and demonstrate that ours enables more accurate registration with fewer local deformations in shorter time.

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  • The JRC2018 unisex template brain used for registration of EM and light microscopy brain images (Bogovic et al., 2020) is about 30% smaller than the hemibrain EM data.

    “the JRC2018 unisex template brain used for the registration of EM and light microscopy brain images (Bogovic et al., 2020) is about 30% smaller (Figure 19(c))”

    From A connectome and analysis of the adult Drosophila central brain (2020) · cites this paper as Bogovic et al., 2020

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Authors
Bogovic, J. A., Otsuna, H., Heinrich, L., Ito, M., Jeter, J., Meissner, G., Nern, A., Colonell, J., Malkesman, O., Ito, K., & Saalfeld, S.
Affiliations
  • Howard Hughes Medical Institute (US)
  • University of Cologne (DE)
Corresponding author
  • Bogovic, John A.
  • Saalfeld, Stephan
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2020-12-31
Volume
15
Issue
12
Pages
e0236495
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0236495
PMC
PMC7774840
OpenAlex
W3114097778
Cited by
131
References
8
Citations by year
2026: 14 · 2025: 27 · 2024: 37 · 2023: 18 · 2022: 10 · 2021: 13 · 2020: 11 · 2019: 1
FWCI
6.31
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Howard Hughes Medical Institute
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Physical Sciences › Computer Science › Computer Vision and Pattern Recognition › Medical Image Segmentation Techniques
Keywords
Drosophila melanogaster, brain template, ventral nerve cord, groupwise registration, template fabrication, artifact removal, image stitching, comparative neuroanatomy, high-resolution imaging
MeSH terms
Animals, Brain, Drosophila melanogaster, Female, Image Processing, Computer-Assisted, Male, Microscopy, Electron, Nerve Tissue, Neurons, Microscopy, Confocal

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