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Cited by 10Visual perception and processing mechanismsNeural dynamics and brain functionZebrafish Biomedical Research Applications

Dimensionality reduction reveals separate translation and rotation populations in the zebrafish hindbrain

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Feierstein, Claudia E., de Goeij, Michelle H.M., Ostrovsky, Aaron D., Laborde, Alexandre, Portugues, Ruben, Orger, Michael B., Machens, Christian K.·2023·Current Biology
DOI 10.1016/j.cub.2023.08.037PMC PMC10524920

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Abstract

In many brain areas, neuronal activity is associated with a variety of behavioral and environmental variables. In particular, neuronal responses in the zebrafish hindbrain relate to oculomotor and swimming variables as well as sensory information. However, the precise functional organization of the neurons has been difficult to unravel because neuronal responses are heterogeneous. Here, we used dimensionality reduction methods on neuronal population data to reveal the role of the hindbrain in visually driven oculomotor behavior and swimming. We imaged neuronal activity in zebrafish expressing GCaMP6s in the nucleus of almost all neurons while monitoring the behavioral response to gratings that rotated with different speeds. We then used reduced-rank regression, a method that condenses the sensory and motor variables into a smaller number of "features," to predict the fluorescence traces of all ROIs (regions of interest). Despite the potential complexity of the visuo-motor transformation, our analysis revealed that a large fraction of the population activity can be explained by only two features. Based on the contribution of these features to each ROI's activity, ROIs formed three clusters. One cluster was related to vergent movements and swimming, whereas the other two clusters related to leftward and rightward rotation. Voxels corresponding to these clusters were segregated anatomically, with leftward and rightward rotation clusters located selectively to the left and right hemispheres, respectively. Just as described in many cortical areas, our analysis revealed that single-neuron complexity co-exists with a simpler population-level description, thereby providing insights into the organization of visuo-motor transformations in the hindbrain.

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  • Together, these subnetworks could provide for maintenance of conjugate and disconjugate eye movements.

    “Together, these subnetworks could provide for maintenance of conjugate and disconjugate eye movements56–58.”

    From Predicting modular functions and neural coding of behavior from a synaptic wiring diagram (2024) · cites this paper as [58]

    The Discussion (page unavailable) identifies separate activity modes related to conjugate and vergent eye movements, supporting the proposed distinction between subnetworks. But the Results (page unavailable) describe the vergence cluster mainly in terms of eye velocity, while eye-position representations in the rotation clusters are conjugate. The paper does not establish that these populations maintain both conjugate and disconjugate eye movements. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

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Authors
Feierstein, C. E., de Goeij, M. H. M., Ostrovsky, A. D., Laborde, A., Portugues, R., Orger, M. B., & Machens, C. K.
Affiliations
Champalimaud Foundation (PT)
Corresponding author
  • Feierstein, Claudia E.
  • Orger, Michael B.
  • Machens, Christian K.
ORCID
Venue
Current Biology
Publisher
Elsevier BV
Published
2023-09
Volume
33
Issue
18
Pages
3911-3925.e6
ISSN
0960-9822 (ISSN-L)
DOI
10.1016/j.cub.2023.08.037
PMC
PMC10524920
OpenAlex
W4386563284
Cited by
10
References
11
Citations by year
2026: 2 · 2025: 2 · 2024: 6
FWCI
1.50
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Biology (publishedVersion, cc-by-nc)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc)
  • Open access LMU (Ludwid Maxmilian's Universitat Munchen) (submittedVersion, cc-by-nc)
  • mediaTUM (Technical University of Munich) (submittedVersion)
Funders
  • European Research Council
  • European Regional Development Fund
  • Fundação Champalimaud (543009)
  • VolkswagenStiftung
  • Deutsche Forschungsgemeinschaft (390857198)
  • Fundação para a Ciência e a Tecnologia (LISBOA-01-0145-FEDER-022170, PTDC/NEU-SCC/5221/2014)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Visual perception and processing mechanisms
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Keywords
neural population activity, oculomotor behavior, swimming behavior, vergence movements, rotation direction, hemispheric lateralization, reduced rank regression, dimensionality reduction, GCaMP6s
MeSH terms
Animals, Brain, Rhombencephalon, Rotation, Swimming, Zebrafish

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