Yeast Rev1 is cell cycle regulated, phosphorylated in response to DNA damage and its binding to chromosomes is dependent upon MEC1
Abstract
Translesion DNA synthesis (TLS) is one of the mechanisms involved in lesion bypass during DNA replication. Three TLS polymerases (Pol) are present in the yeast Saccharomyces cerevisiae: Pol zeta, Pol eta and the product of the REV1 gene. Rev1 is considered a deoxycytidyl transferase because it almost exclusively inserts a C residue in front of the lesion. Even though REV1 is required for most of the UV-induced and spontaneous mutagenesis events, the role of Rev1 is poorly understood since its polymerase activity is often dispensable. Rev1 interacts with several TLS polymerases in mammalian cells and may act as a platform in the switching mechanism required to substitute a replicative polymerase with a TLS polymerase at the sites of DNA lesions. Here we show that yeast Rev1 is a phosphoprotein, and the level of this modification is cell cycle regulated under normal growing conditions. Rev1 is unphosphorylated in G1, starts to be modified while cells are passing S phase and it becomes hyper-phosphorylated in mitosis. Rev1 is also hyper-phosphorylated in response to a variety of DNA damaging agents, including treatment with a radiomimetic drug mostly causing double-strand breaks (DSB). By using the chromosome spreading technique we found the Rev1 is bound to chromosomes throughout the cell cycle, and its binding does not significantly increase in response to genotoxic stress. Therefore, Rev1 phosphorylation does not appear to modulate its binding to chromosomes, suggesting that such modification may influence other aspects of the TLS process. Rev1 binding under damaged and undamaged conditions, is at least partially dependent on MEC1, a gene playing a pivotal role in the DNA damage checkpoint cascade. This genetic dependency may suggest a role for MEC1 in spontaneous mutagenesis events, which require a functional REV1 gene.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
An active checkpoint leads to Rev1 phosphorylation, possibly increasing TLS activity and reducing RPA-covered ssDNA, promoting checkpoint switch-off.
“An active checkpoint leads to Rev1 phosphorylation [86,87], possibly increasing TLS activity and progressively reducing the amount of RPA-covered ssDNA, thus promoting the switch off of the checkpoint itself.”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [87]
Cites
Friedberg, E. C., Walker, G. C., Siede, W., Wood, R. D., Schultz, R. A., & Ellenberger, T. (2005) · doi:10.1128/9781555816704 · Cited by 5,683 · see citing works
Longtine, M. S., Mckenzie, A., DeMarini, D. J., Shah, N. G., Wach, A., Brachat, A., Philippsen, P., & Pringle, J. R. (1998) · Yeast · doi:10.1002/(sici)1097-0061(199807)14:10 · Cited by 5,367 · see citing works
Sherman, F. (1991) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(91)94004-v · Cited by 4,078 · see citing works
Bootsma, D., & Hoeijmakers, J. H. J. (1996) · Trends in Genetics · doi:10.1016/0168-9525(96)81408-9 · Cited by 2,285 · see citing works
RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
Hoege, C., Pfander, B., Moldovan, G., Pyrowolakis, G., & Jentsch, S. (2002) · Nature · doi:10.1038/nature00991 · Cited by 2,202 · see citing works
The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase η
Masutani, C., Kusumoto, R., Yamada, A., Dohmae, N., Yokoi, M., Yuasa, M., Araki, M., Iwai, S., Takio, K., & Hanaoka, F. (1999) · Nature · doi:10.1038/21447 · Cited by 1,292 · see citing works
EUKARYOTIC TRANSLESION SYNTHESIS DNA POLYMERASES: Specificity of Structure and Function
Prakash, S., Johnson, R. E., & Prakash, L. (2005) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.74.082803.133250 · Cited by 999 · see citing works
Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugation
Stelter, P., & Ulrich, H. D. (2003) · Nature · doi:10.1038/nature01965 · Cited by 849 · see citing works
Interaction of Human DNA Polymerase η with Monoubiquitinated PCNA
Kannouche, P. L., Wing, J., & Lehmann, A. R. (2004) · Molecular Cell · doi:10.1016/s1097-2765(04)00259-x · Cited by 845 · see citing works
Efficient Bypass of a Thymine-Thymine Dimer by Yeast DNA Polymerase, Polη
Johnson, R. E., Prakash, S., & Prakash, L. (1999) · Science · doi:10.1126/science.283.5404.1001 · Cited by 736 · see citing works
<i>hRAD30</i> Mutations in the Variant Form of Xeroderma Pigmentosum
Johnson, R. E., Kondratick, C. M., Prakash, S., & Prakash, L. (1999) · Science · doi:10.1126/science.285.5425.263 · Cited by 732 · see citing works
Zhao, X., Muller, E. G. D., & Rothstein, R. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80277-4 · Cited by 728 · see citing works
Eukaryotic polymerases ι and ζ act sequentially to bypass DNA lesions
Johnson, R. E., Washington, M. T., Haracska, L., Prakash, S., & Prakash, L. (2000) · Nature · doi:10.1038/35023030 · Cited by 632 · see citing works
Deoxycytidyl transferase activity of yeast REV1 protein
Nelson, J. R., Lawrence, C. W., & Hinkle, D. C. (1996) · Nature · doi:10.1038/382729a0 · Cited by 573 · see citing works
Lopes, M., Foiani, M., & Sogo, J. M. (2006) · Molecular Cell · doi:10.1016/j.molcel.2005.11.015 · Cited by 563 · see citing works
Watanabe, K., Tateishi, S., Kawasuji, M., Tsurimoto, T., Inoue, H., & Yamaizumi, M. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600383 · Cited by 538 · see citing works
Trading Places: How Do DNA Polymerases Switch during Translesion DNA Synthesis?
Friedberg, E. C., Lehmann, A. R., & Fuchs, R. P. (2005) · Molecular Cell · doi:10.1016/j.molcel.2005.03.032 · Cited by 376 · see citing works
Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis
Guo, C., Fischhaber, P. L., Luk‐Paszyc, M. J., Masuda, Y., Zhou, J., Kamiya, K., Kisker, C., & Friedberg, E. C. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg626 · Cited by 353 · see citing works
Interaction of hREV1 with three human Y‐family DNA polymerases
Ohashi, E., Murakumo, Y., Kanjo, N., Akagi, J., Masutani, C., Hanaoka, F., & Ohmori, H. (2004) · Genes to Cells · doi:10.1111/j.1356-9597.2004.00747.x · Cited by 256 · see citing works
Interactions in the Error-prone Postreplication Repair Proteins hREV1, hREV3, and hREV7
Murakumo, Y., Ogura, Y., Ishii, H., Numata, S., Ichihara, M., Croce, C. M., Fishel, R. A., & Takahashi, M. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102051200 · Cited by 230 · see citing works
REV1 Protein Interacts with PCNA: Significance of the REV1 BRCT Domain In Vitro and In Vivo
Guo, C., Sonoda, E., Tang, T.-S., Parker, J. L., Bielen, A. B., Takeda, S., Ulrich, H. D., & Friedberg, E. C. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.05.038 · Cited by 213 · see citing works
Tissier, A., Kannouche, P. L., Reck, M.-P., Lehmann, A. R., Fuchs, R. P., & Cordonnier, A. M. (2004) · DNA repair · doi:10.1016/j.dnarep.2004.06.015 · Cited by 207 · see citing works
Cellular roles of DNA polymerase ζ and Rev1 protein
Lawrence, C. W. (2002) · DNA repair · doi:10.1016/s1568-7864(02)00038-1 · Cited by 202 · see citing works
A Role for REV3 in Mutagenesis During Double-Strand Break Repair in Saccharomyces cerevisiae
Holbeck, S. L., & Strathern, J. N. (1997) · Genetics · doi:10.1093/genetics/147.3.1017 · Cited by 198 · see citing works
Simpson, L. J., & Sale, J. E. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg161 · Cited by 178 · see citing works
Waters, L. S., & Walker, G. C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0510167103 · Cited by 171 · see citing works
DNA damage checkpoint in budding yeast
Longhese, M. P., Foiani, M., Muzi-Falconi, M., Lucchini, G., & Plevani, P. (1998) · The EMBO Journal · doi:10.1093/emboj/17.19.5525 · Cited by 162 · see citing works
Self-citationEsposito, G., Godin, I., Klein, U., Yaspo, M., Cumano, A., & Rajewsky, K. (2000) · Current Biology · doi:10.1016/s0960-9822(00)00726-0 · Cited by 161 · see citing works
Checkpoint activation regulates mutagenic translesion synthesisPreprint
Kai, M., & Wang, T. S. ‐. (2003) · Genes & Development · doi:10.1101/gad.1043203 · Cited by 157 · see citing works
Longhese, M. P., Paciotti, V., Fraschini, R., Zaccarini, R., Plevani, P., & Lucchini, G. (1997) · The EMBO Journal · doi:10.1093/emboj/16.17.5216 · Cited by 149 · see citing works
Self-citationVertebrate DNA damage tolerance requires the C-terminus but not BRCT or transferase domains of REV1
Ross, A.-L. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki279 · Cited by 144 · see citing works
Replication of damaged DNA by translesion synthesis in human cells
Lehmann, A. R. (2004) · FEBS Letters · doi:10.1016/j.febslet.2004.11.029 · Cited by 142 · see citing works
Paulovich, A. G., Armour, C. D., & Hartwell, L. H. (1998) · Genetics · doi:10.1093/genetics/150.1.75 · Cited by 133 · see citing works
DNA damage checkpoints and DNA replication controls in Saccharomyces cerevisiae
Foiani, M., Pellicioli, A., Lopes, M., Lucca, C., Ferrari, M., Liberi, G., Muzi-Falconi, M., & Plevani, P. (2000) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/s0027-5107(00)00049-x · Cited by 130 · see citing works
Self-citationHarfe, B. D., & Jinks-Robertson, S. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)00145-3 · Cited by 120 · see citing works
Sabbioneda, S., Minesinger, B. K., Giannattasio, M., Plevani, P., Muzi-Falconi, M., & Jinks-Robertson, S. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m507638200 · Cited by 116 · see citing works
Self-citationPhysical and functional interactions between nucleotide excision repair and DNA damage checkpoint
Giannattasio, M., Lazzaro, F., Longhese, M. P., Plevani, P., & Muzi‐Falconi, M. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600051 · Cited by 113 · see citing works
Self-citationMultiple Roles of Vertebrate REV Genes in DNA Repair and Recombination
Okada, T., Sonoda, E., Yoshimura, M., Kawano, Y., Saya, H., Kohzaki, M., & Takeda, S. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.14.6103-6111.2005 · Cited by 112 · see citing works
Takata, H., Kanoh, Y., Gunge, N., Shirahige, K., & Matsuura, A. (2004) · Molecular Cell · doi:10.1016/s1097-2765(04)00262-x · Cited by 95 · see citing works
Hirano, Y., & Sugimoto, K. (2006) · Current Biology · doi:10.1016/j.cub.2006.01.063 · Cited by 81 · see citing works
Otsuka, C., Kunitomi, N., Iwai, S., Loakes, D., & Negishi, K. (2005) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2005.03.005 · Cited by 52 · see citing works
Muzi-Falconi, M., Piseri, A., Ferrari, M., Lucchini, G., Plevani, P., & Foiani, M. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.22.10519 · Cited by 50 · see citing works
Self-citationPathways of ultraviolet mutability in Saccharomyces cerevisiae
Lemontt, J. F. (1971) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(71)90041-8 · Cited by 42 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Y-family DNA polymerases and their role in tolerance of cellular DNA damage
Sale, J. E., Lehmann, A. R., & Woodgate, R. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3289 · Cited by 665 · see citing works
“An additional level of complexity is introduced by the observation that the Y-family polymerases can themselves be post-translationally modified by ubiquitylation, phosphorylation and possibly SUMOylatio”
Eukaryotic Translesion Polymerases and Their Roles and Regulation in DNA Damage Tolerance
Waters, L. S., Minesinger, B. K., Wiltrout, M. E., D'Souza, S., Woodruff, R. V., & Walker, G. C. (2009) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00034-08 · Cited by 591 · see citing works
What a difference a decade makes: Insights into translesion DNA synthesis
Yang, W., & Woodgate, R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0704219104 · Cited by 377 · see citing works
Translesion DNA Synthesis and Mutagenesis in Eukaryotes
Sale, J. E. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a012708 · Cited by 282 · see citing works
“Both REV1 and Polh are phosphorylated by ATR, although how this regulates TLS is still unclear (Sabbioneda et al. 2007; Pages et al. 2009; Gohler et al. 2011).”
BackgroundKarras, G. I., & Jentsch, S. (2010) · Cell · doi:10.1016/j.cell.2010.02.028 · Cited by 282 · see citing works
“In fact, TLS (but not error-free DDT) seems to be stimulated by the Mec1/ATR-dependent checkpoint response (Pagès et al., 2009; Paulovich et al., 1998; Sabbioneda et al., 2007; Sabbioneda et al., 2005).”
DNA damage kinase signaling: checkpoint and repair at 30 years
Lanz, M. C., Dibitetto, D., & Smolka, M. B. (2019) · The EMBO Journal · doi:10.15252/embj.2019101801 · Cited by 259 · see citing works
“This complements earlier studies, where it was shown that Mec1 is essential for Rev1 binding to chromosomes (Hirano & Sugimoto, 2006; Sabbioneda et al , 2007).”
Translesion DNA polymerases in eukaryotes: what makes them tick?
Vaisman, A., & Woodgate, R. (2017) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/10409238.2017.1291576 · Cited by 247 · see citing works
“Damage-dependent phosphorylation has also been reported for Rev1 (Pages et al., 2009; Sabbioneda et al., 2007) (Figure 3(C).”
BackgroundEdmunds, C. E., Simpson, L. J., & Sale, J. E. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.03.024 · Cited by 239 · see citing works
“(A) An encounter between the replicative polymerases and a stalling DNA lesion results in the recruitment of REV1 or the alteration of a preexisting complex containing REV1, possibly in response the ubiquitination or phosphorylation of proteins adjacent to the replisome (Hirano and Sugimoto, 2006; Sabbioneda et al., 2…”
BackgroundInfluentialDNA Repair Mechanisms and the Bypass of DNA Damage in Saccharomyces cerevisiae
Boiteux, S., & Jinks-Robertson, S. (2013) · Genetics · doi:10.1534/genetics.112.145219 · Cited by 227 · see citing works
“Finally, Rev1 is hyper-phosphorylated during mitosis and after DNA damage, suggesting potential posttranslational regulation of its activity (Sabbioneda et al. 2007).”
BackgroundRNase H and Postreplication Repair Protect Cells from Ribonucleotides Incorporated in DNA
Lazzaro, F., Novarina, D., Amara, F., Watt, D. L., Stone, J. E., Costanzo, V., Burgers, P., Kunkel, T. A., Plevani, P., & Muzi-Falconi, M. (2012) · Molecular Cell · doi:10.1016/j.molcel.2011.12.019 · Cited by 177 · see citing works
“To monitor protein levels and phosphorylation, TCA protein extracts were prepared and analyzed by SDS-PAGE (Sabbioneda et al., 2007); western blots were performed with anti-Rad53, anti-H2A, and anti-gH2AX antibodies.”
MethodSelf-citationMakarova, А. V., & Burgers, P. (2015) · DNA repair · doi:10.1016/j.dnarep.2015.02.012 · Cited by 150 · see citing works
Y-family DNA polymerases in mammalian cells
Guo, C., Kosarek, J. N., Tang, T.-S., & Friedberg, E. C. (2009) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-009-0024-4 · Cited by 142 · see citing works
Checkpoint mechanisms at the intersection between DNA damage and repair
Lazzaro, F., Giannattasio, M., Puddu, F., Granata, M., Pellicioli, A., Plevani, P., & Muzi-Falconi, M. (2009) · DNA Repair · doi:10.1016/j.dnarep.2009.04.022 · Cited by 94 · see citing works
Self-citationATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage
Göhler, T., Sabbioneda, S., Green, C., & Lehmann, A. R. (2011) · The Journal of Cell Biology · doi:10.1083/jcb.201008076 · Cited by 87 · see citing works
“Damage-dependent phosphorylation of the Y-family member Rev1 in Saccharomyces cerevisiae has been reported by two groups (Sabbioneda et al., 2007; Pagès et al., 2009).”
BackgroundSelf-citationReconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart
Guilliam, T. A., & Yeeles, J. T. P. (2020) · Nature Structural & Molecular Biology · doi:10.1038/s41594-020-0418-4 · Cited by 81 · see citing works
Timing and spacing of ubiquitin‐dependent DNA damage bypass
Ulrich, H. D. (2011) · FEBS Letters · doi:10.1016/j.febslet.2011.05.028 · Cited by 73 · see citing works
“Moreover, its phosphorylation pattern suggests a particular relevance of the postreplicative stage: phosphorylation of Rev1, which enhances the efficiency of damage-induced mutagenesis in NER-deficient cells, is induced by DNA damage, but is also cell cycle-regulated with a maximum in mitosis [39].”
BackgroundMadia, F., Wei, M., Yuan, V., Hu, J. H., Gattazzo, C., Pham, P., Goodman, M. F., & Longo, V. D. (2009) · The Journal of Cell Biology · doi:10.1083/jcb.200906011 · Cited by 73 · see citing works
“Rev1 expression is threefold higher during G2/M than in G1 phase (Waters and Walker, 2006; Sabbioneda et al., 2007).”
BackgroundSchenten, D., Kracker, S., Esposito, G., Franco, S., Klein, U., Murphy, M., Alt, F. W., & Rajewsky, K. (2009) · The Journal of Experimental Medicine · doi:10.1084/jem.20080669 · Cited by 70 · see citing works
Emerging strategies for cancer therapy by ATR inhibitors
Yano, K., & Shiotani, B. (2023) · Cancer Science · doi:10.1111/cas.15845 · Cited by 64 · see citing works
Pagès, V., Santa Maria, S. R., Prakash, L., & Prakash, S. (2009) · Genes & Development · doi:10.1101/gad.1793409 · Cited by 55 · see citing works
Send in the Clamps: Control of DNA Translesion Synthesis in Eukaryotes
Jansen, J. G., Fousteri, M. I., & de Wind, N. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.11.005 · Cited by 54 · see citing works
Reversal of PCNA Ubiquitylation by Ubp10 in Saccharomyces cerevisiae
Gallego-Sánchez, A., Andrés, S., Conde, F., San-Segundo, P. A., & Bueno, A. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002826 · Cited by 52 · see citing works
Conde, F., & San-Segundo, P. A. (2008) · Genetics · doi:10.1534/genetics.108.089003 · Cited by 51 · see citing works
“In response to UV damage, physical interaction with the PCNA-like 9-1-1 checkpoint complex and Mec1-dependent phosphorylation is required for chromatin association of Polz-Rev1 (Sabbioneda et al. 2005, 2007); however, the effect of MMS has not been analyzed.”
BackgroundShim, H. S., Wei, M., Brandhorst, S., & Longo, V. D. (2015) · Cancer Research · doi:10.1158/0008-5472.can-14-2249 · Cited by 48 · see citing works
“Previous studies have shown that REV1 is modified by phosphorylation in yeast and by ubiquitination in mammalian cells (28,46), but its SUMOylation had not been described.”
Mind the gap: keeping UV lesions in check
Novarina, D., Amara, F., Lazzaro, F., Plevani, P., & Muzi-Falconi, M. (2011) · DNA Repair · doi:10.1016/j.dnarep.2011.04.030 · Cited by 43 · see citing works
Self-citationRegulation of tolerance to DNA alkylating damage by Dot1 and Rad53 in Saccharomyces cerevisiae
Conde, F., Ontoso, D., Acosta, I., Gallego-Sánchez, A., Bueno, A., & San-Segundo, P. A. (2010) · DNA repair · doi:10.1016/j.dnarep.2010.07.003 · Cited by 39 · see citing works
Roles of mutagenic translesion synthesis in mammalian genome stability, health and disease
Jansen, J. G., Tsaalbi‐Shtylik, A., & de Wind, N. (2015) · DNA repair · doi:10.1016/j.dnarep.2015.01.001 · Cited by 39 · see citing works
Chun, A. C. S., Kok, K., & Jin, D. (2013) · Cell Cycle · doi:10.4161/cc.23214 · Cited by 38 · see citing works
de Sousa, M. M. L., Zub, K. A., Aas, P. A., Hanssen‐Bauer, A., Demirović, A., Sarno, A., Tian, E., Liabakk, N., & Slupphaug, G. (2013) · PLoS ONE · doi:10.1371/journal.pone.0055493 · Cited by 37 · see citing works
“This is important when DNA repair proteins are the subject of analysis, since the expression of many proteins involved in DNA damage signaling and repair are strictly cell cycle regulated [37–43].”
BackgroundPTIP/Swift is required for efficient PCNA ubiquitination in response to DNA damage
Göhler, T., Munoz, I. M., Rouse, J. W., & Blow, J. J. (2008) · DNA repair · doi:10.1016/j.dnarep.2008.02.001 · Cited by 37 · see citing works
Molecular Chaperone Hsp90 Regulates REV1-Mediated Mutagenesis
Pozo, F. M., Oda, T., Sekimoto, T., Murakumo, Y., Masutani, C., Hanaoka, F., & Yamashita, T. (2011) · Molecular and Cellular Biology · doi:10.1128/mcb.05117-11 · Cited by 33 · see citing works
“While emerging evidence indicates that posttranslational control of REV1 plays an important regulatory role in yeast (20, 44, 49, 60), little is known concerning the regulatory mechanisms of REV1 in mammalian cells.”
BackgroundKozmin, S. G., & Jinks-Robertson, S. (2013) · Genetics · doi:10.1534/genetics.112.147421 · Cited by 31 · see citing works
“In our system, however, it is unlikely that Mec1-dependent phosphorylation of Rev1 is important for the production of two-strand mutations in G0 cells; the rev1-S31A mutant was as UV-mutable as the WT strain (Figure 6).”
BackgroundInfluentialUBR5 interacts with the replication fork and protects DNA replication from DNA polymerase η toxicity
Cipolla, L., Bertoletti, F., Maffia, A., Liang, C., Lehmann, A. R., Cohn, M. A., & Sabbioneda, S. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz824 · Cited by 30 · see citing works
“In addition, TLS polymerases, in particular pol (cid:2) , are themselves phosphorylated (14–16), SUMOylated (17) and ubiquitylated (11–13) and this last post translational modification is thought to prevent their erroneous recruitment to the chromatin when they are not needed (13).”
Self-citationPhosphorylation regulates human polη stability and damage bypass throughout the cell cycle
Bertoletti, F., Cea, V., Liang, C., Lanati, T., Maffia, A., Avarello, M. D. M., Cipolla, L., Lehmann, A. R., Cohn, M. A., & Sabbioneda, S. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx619 · Cited by 23 · see citing works
Self-citationREV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis
Chen, Y., Jie, X., Xing, B., Wu, Z., Yang, X., Rao, X., Xu, Y., Zhou, D., Dong, X., Zhang, T., et al. (2022) · Cell Death and Disease · doi:10.1038/s41419-022-04567-5 · Cited by 23 · see citing works
“REV1, the central member of the TLS polymerase family, mediates the error-prone DNA damage tolerance mechanism and participates in various endogenous and exogenous DNA damage responses [27, 28].”
BackgroundPanigada, D., Grianti, P., Nespoli, A., Rotondo, G., Castro, D. G., Quadri, R., Piatti, S., Plevani, P., & Muzi-Falconi, M. (2013) · Developmental Cell · doi:10.1016/j.devcel.2013.07.013 · Cited by 23 · see citing works
“(legend continued on next page) Haspin Kinase and Mitotic Cell Polarity Developmental Cell 26, 1–13, September 16, 2013 ª2013 Elsevier Inc. 3 Haspin Kinase and Mitotic Cell Polarity MAD2 gene, a central factor in the spindle assembly checkpoint (SAC) elicited by microtubule disassembly.”
MethodInfluentialSelf-citationProteasomal regulation of the mutagenic translesion DNA polymerase, Saccharomyces cerevisiae Rev1
Wiltrout, M. E., & Walker, G. C. (2011) · DNA repair · doi:10.1016/j.dnarep.2010.10.008 · Cited by 22 · see citing works
DNA polymerase ζ is essential for hexavalent chromium-induced mutagenesis
O’Brien, T. J., Witcher, P., Brooks, B., & Patierno, S. R. (2009) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2009.01.012 · Cited by 22 · see citing works
Fasullo, M., Sun, M., & Egner, P. A. (2008) · Molecular Carcinogenesis · doi:10.1002/mc.20417 · Cited by 20 · see citing works
“merase) accumulates during late S and G2 phases of the cell-cycle [28,29].”
BackgroundDNA damage bypass pathways and their effect on mutagenesis in yeast
Arbel, M., Liefshitz, B., & Kupiec, M. (2020) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuaa038 · Cited by 19 · see citing works
Sml1 Inhibits the DNA Repair Activity of Rev1 in Saccharomyces cerevisiae during Oxidative Stress
Yao, R., Zhou, P., Wu, C., Liu, L., & Wu, J. (2020) · Applied and Environmental Microbiology · doi:10.1128/aem.02838-19 · Cited by 16 · see citing works
“cerevisiae, cells lacking REV1 are sensitive to DNA damaging agents, including cisplatin, mitomycin C, and UV radiation (37).”
BackgroundResultInfluentialFasullo, M., Chen, Y., Bortcosh, W., Sun, M., & Egner, P. A. (2010) · Journal of Nucleic Acids · doi:10.4061/2010/456487 · Cited by 16 · see citing works
Uchiyama, M., Terunuma, J., & Hanaoka, F. (2015) · PLoS ONE · doi:10.1371/journal.pone.0130000 · Cited by 11 · see citing works
“Mec1, a budding yeast homolog of ataxia telangiectasia and Rad3-related protein (ATR), phosphorylates Rev1 at its N-terminus, thereby increasing the association of Rev1 with chromatin [21, 22].”
BackgroundEukaryotic stress–induced mutagenesis is limited by a local control of translesion synthesis
Masłowska, K. H., Villafañez, F., Laureti, L., Iwai, S., & Pagès, V. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac044 · Cited by 10 · see citing works
Sobolewska, A., Hałas, A., Płachta, M., McIntyre, J., & Śledziewska-Gójska, E. (2020) · Current Genetics · doi:10.1007/s00294-020-01061-3 · Cited by 9 · see citing works
“Checkpoint activity has been reported to participate in the hyperphosphorylation of Rev1 (Sabbioneda et al. 2007).”
BackgroundGallego-Sánchez, A., Ufano, S., Andrés, S., & Bueno, A. (2013) · PLoS ONE · doi:10.1371/journal.pone.0081108 · Cited by 8 · see citing works
“mutants [39,40] suggesting that Mec1 not only regulates the association of Rev1 and polymerase f with chromatin in response”
BackgroundPost-replicative lesion processing limits DNA damage-induced mutagenesis
Masłowska, K. H., Wong, R. P. C., Ulrich, H. D., & Pagès, V. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf198 · Cited by 9 · see citing works
Alternative splicing of two translesion synthesis DNA polymerases from Arabidopsis thaliana
Santiago, M. J., Alejandre-Durán, E., & Ruiz‐Rubio, M. (2009) · Plant Science · doi:10.1016/j.plantsci.2009.01.018 · Cited by 5 · see citing works
Haspin Modulates the G2/M Transition Delay in Response to Polarization Failures in Budding Yeast
Galli, M. G., Diani, L., Quadri, R., Nespoli, A., Galati, E., Panigada, D., Plevani, P., & Muzi-Falconi, M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.625717 · Cited by 5 · see citing works
Self-citationHuman REV3L: Expression and protein interaction studies
Gan, G. N. (2007) · D-Scholarship@Pitt (University of Pittsburgh) · Cited by 3 · see citing works
A Synthetic Interaction betweenCDC20andRAD4inSaccharomyces cerevisiaeupon UV Irradiation
Connors, B. J., Rochelle, L. K., Roberts, A., & Howard, G. (2014) · Molecular Biology International · doi:10.1155/2014/519290 · Cited by 3 · see citing works
“…Article A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation Bernadette Connors,1 Lauren Rochelle,2,3 Asela Roberts,3 and Graham Howard3,4 1 Department of Biology, Dominican College, 470 Western Highway, Orangeburg, NY 10962, USA 2Department of Cell…”
BackgroundLatoszek, M., Bagińska, K., Śledziewska-Gójska, E., & Kaniak, A. (2024) · Scientific Reports · doi:10.1038/s41598-024-82104-4 · Cited by 1 · see citing works
“Rev1 has been shown to be phosphorylated in a manner dependent on the Mec1 kinase 51,52 .”
Checkpoint Control of DNA Repair in Yeast
Fasullo, M. (2021) · IntechOpen eBooks · doi:10.5772/intechopen.96966 · Cited by 1 · see citing works
“Mec1, which increases its affinity to ssDNA [26].”
BackgroundStudies of cohesin functions in the yeast and human DNA damage response
Enervald, E. (2013) · Figshare
Translesion DNA Synthesis and Hsp90
Yamashita, T., Oda, T., & Sekimoto, T. (2012) · Genes and Environment · doi:10.3123/jemsge.34.89
DNA Damage Tolerance and Translesion Synthesis
Lehmann, A. R. (2009) · Springer eBooks · doi:10.1007/978-90-481-2561-6_10
Coping with DNA Damage and Replication Stress
Ulrich, H. D. (2009) · Royal Society of Chemistry eBooks · doi:10.1039/9781847559852-00178
Interaction of the NuA4 Complex and Chromatin Modifications with the DNA Damage Tolerance Pathway
Renaud-Young, M. M. (2015) · Libraries and Cultural Resources (University of Calgary) · doi:10.11575/prism/26597
Chromatin Modifications in DNA Repair and Cancer
Renaud-Young, M. M., Riabowol, K. T., & Cobb, J. A. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-803309-8.00028-8
Feu, S. (2019) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)
(2007) · Yeast · doi:10.1002/yea.1453
Tsanov, N. (2012) · theses.fr (ABES) · doi:10.70675/2122f712z2f95z45caz8f82za68872f01e3c
Caractérisation fonctionnelle de la tolérance aux endommagements de l’ADN des embryons précoces
Furno, E. L. (2019) · theses.fr (ABES) · doi:10.70675/cf32bcd5z3031z4693z8b1bz62fe76673ccc
- Authors
- Sabbioneda, S., Bortolomai, I., Giannattasio, M., Plevani, P., & Muzi-Falconi, M.
- Affiliations
- University of Milan (IT)
- Corresponding author
- Plevani, Paolo
- Muzi-Falconi, Marco
- Venue
- DNA Repair
- Publisher
- Elsevier BV
- Published
- 2007-01
- Volume
- 6
- Issue
- 1
- Pages
- 121-127
- ISSN
- 1568-7856 (ISSN-L)
- 1568-7864
- OpenAlex
- W2072289417
- Cited by
- 65
- References
- 43
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 4 · 2017: 2 · 2016: 1 · 2015: 5 · 2014: 1 · 2013: 8 · 2012: 5
- FWCI
- 2.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- DNA repair (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Commission
- Associazione Italiana per la Ricerca sul Cancro
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Rev1, translesion synthesis, DNA damage response, phosphorylation, cell cycle regulation, Mec1, DNA damage checkpoint, UV mutagenesis, spontaneous mutagenesis, Saccharomyces cerevisiae, DNA double-strand breaks, radiomimetic agents
- MeSH terms
- Y-Family DNA Polymerases, DNA Damage, DNA-Directed DNA Polymerase, DNA Repair, DNA, Fungal, Mitosis, Nucleotidyltransferases, Phosphoproteins, Phosphorylation, Saccharomyces cerevisiae, Chromosomes, Fungal, G1 Phase, S Phase, Protein Serine-Threonine Kinases, Saccharomyces cerevisiae Proteins, Intracellular Signaling Peptides and Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
