In vivo fate of unimers and micelles of a poly(ethylene glycol)-block-poly(caprolactone) copolymer in mice following intravenous administration
Abstract
Methoxy poly(ethylene glycol)-b-poly(caprolactone) (MePEG-b-PCL) copolymers with varying PEG block lengths and a constant PCL block length were synthesized by cationic ring-opening polymerization and used to form nano-sized micelles. Due to their small size and superior in vitro stability, the MePEG(5000)-b-PCL(5000) micelles were selected for further in vitro characterization and an in vivo evaluation of their fate and stability following intravenous (i.v.) administration. Specifically, (3)H-labelled MePEG(5000)-b-PCL(5000) micelles were i.v. administered to Balb/C mice at copolymer doses of 250, 2 and 0.2 mg/kg in order to examine the distribution kinetics of (1) copolymer assembled as thermodynamically stable micelles, (2) copolymer assembled as thermodynamically unstable micelles and (3) copolymer unimers, respectively. Overall, it was found that when the copolymer is assembled as thermodynamically stable micelles the material is effectively restricted to the plasma compartment. Interestingly, the copolymer was found to have a relatively long circulation half-life even when administered at a dose that would likely fall to concentrations below the CMC following distribution. Analysis of plasma samples from this group revealed that even 24 h post-administration a significant portion of the copolymer remained assembled as intact micelles. In this way, this study demonstrates that the hydrophobic and semi-crystalline nature of the PCL core imparts a high degree of kinetic stability to this micelle system.
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Size exclusion chromatography techniques have been used to differentiate between encapsulated and non-encapsulated drug and, in combination with other techniques, to evaluate the integrity of micelles in plasma.
“These techniques has been used to differentiate between encapsulated and non-encapsulated drug [238] and, in combination with other techniques, to evaluate the integrity of micelles in plasma [239].”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [238]
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- Authors
- Liu, J., Zeng, F., & Allen, C.
- Affiliations
- University of Toronto (CA)
- Corresponding author
- Allen, Christine
- ORCID
- Allen, Christine
- Venue
- European Journal of Pharmaceutics and Biopharmaceutics
- Publisher
- Elsevier BV
- Published
- 2007-03
- Volume
- 65
- Issue
- 3
- Pages
- 309-319
- ISSN
- 0939-6411 (ISSN-L)
- OpenAlex
- W1986339830
- Cited by
- 183
- References
- 32
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 8 · 2022: 7 · 2021: 7 · 2020: 11 · 2019: 9 · 2018: 2 · 2017: 10 · 2016: 12 · 2015: 8 · 2014: 18 · 2013: 9 · 2012: 16
- FWCI
- 4.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Pharmaceutics and Biopharmaceutics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Canadian Institutes of Health Research
- Natural Sciences and Engineering Research Council of Canada
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Physical Sciences › Chemistry › Organic Chemistry › Advanced Polymer Synthesis and Characterization
- Keywords
- micelles, intravenous administration, tissue distribution, circulation half-life, micelle stability, critical micelle concentration, cationic ring-opening polymerization, plasma protein binding
- MeSH terms
- Animals, Biocompatible Materials, Drug Carriers, Half-Life, Injections, Intravenous, Kidney, Liver, Lung, Mice, Inbred BALB C, Micelles, Myocardium, Polyesters, Polyethylene Glycols, Protein Binding, Spleen, Thermodynamics, Tissue Distribution, Tritium, Mice, Nanoparticles
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