Different physiological and behavioural effects of e-cigarette vapour and cigarette smoke in mice
Open accessAbstract
Nicotine is the primary addictive substance in tobacco smoke and electronic cigarette (e-cig) vapour. Methodological limitations have made it difficult to compare the role of the nicotine and non-nicotine constituents of tobacco smoke. The aim of this study was to compare the effects of traditional cigarette smoke and e-cig vapour containing the same amount of nicotine in male BALB/c mice exposed to the smoke of 21 cigarettes or e-cig vapour containing 16.8 mg of nicotine delivered by means of a mechanical ventilator for three 30-min sessions/day for seven weeks. One hour after the last session, half of the animals were sacrificed for neurochemical analysis, and the others underwent mecamylamine-precipitated or spontaneous withdrawal for the purposes of behavioural analysis. Chronic intermittent non-contingent, second-hand exposure to cigarette smoke or e-cig vapour led to similar brain cotinine and nicotine levels, similar urine cotinine levels and the similar up-regulation of α4β2 nicotinic acetylcholine receptors in different brain areas, but had different effects on body weight, food intake, and the signs of mecamylamine-precipitated and spontaneous withdrawal episodic memory and emotional responses. The findings of this study demonstrate for the first time that e-cig vapour induces addiction-related neurochemical, physiological and behavioural alterations. The fact that inhaled cigarette smoke and e-cig vapour have partially different dependence-related effects indicates that compounds other than nicotine contribute to tobacco dependence.
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Short-term studies of electronic cigarette aerosols in adult mice show pulmonary and behavioral effects resembling those from conventional nicotine.
“shorter-term studies of exposures to electronic cigarette-derived aerosols in adult mouse models demonstrate that electronic cigarettes can produce pulmonary and behavioral effects similar to those seen with conventional nicotine exposures.”
From Developmental toxicity of nicotine: A transdisciplinary synthesis and implications for emerging tobacco products (2017) · cites this paper as [27]
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- Authors
- Ponzoni, L., Moretti, M., Sala, M., Fasoli, F., Mucchietto, V, Lucini, V, Cannazza, G., Gallesi, G., Castellana, C., Clementi, F., Zoli, M., Gotti, C., & Braida, D.
- Affiliations
- University of Milan (IT)
- University of Modena and Reggio Emilia (IT)
- Corresponding author
- Gotti, C
- Venue
- European Neuropsychopharmacology
- Publisher
- Elsevier BV
- Published
- 2015-10
- Volume
- 25
- Issue
- 10
- Pages
- 1775-1786
- ISSN
- 0924-977X (ISSN-L)
- OpenAlex
- W2111746394
- Cited by
- 91
- References
- 49
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 2 · 2023: 5 · 2022: 11 · 2021: 10 · 2020: 13 · 2019: 12 · 2018: 8 · 2017: 10 · 2016: 7 · 2015: 2
- FWCI
- 6.63
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- European Neuropsychopharmacology (publishedVersion)
- PubMed (publishedVersion)
- IRIS UNIMORE (University of Modena and Reggio Emilia) (submittedVersion, cc-by-nc-nd)
- Funders
- Ministero della Salute (RF 2009, RF2009‐1549619)
- Regione Lombardia (30263049, 18099/RCC)
- Topics
- Health Sciences › Medicine › Physiology › Smoking Behavior and Cessation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Substance Exposure Effects
- Keywords
- cigarette smoke, nicotine, tobacco dependence, nicotine dependence, cotinine, α4β2 nicotinic acetylcholine receptor, spontaneous withdrawal, episodic memory, emotional responses, body weight, dietary intake
- MeSH terms
- Animals, Body Weight, Brain, Cotinine, Disease Models, Animal, Eating, Emotions, Male, Mecamylamine, Memory, Short-Term, Mice, Inbred BALB C, Nicotine, Receptors, Nicotinic, Respiration, Artificial, Substance Withdrawal Syndrome, Tobacco Smoke Pollution, Tobacco Use Disorder, Nicotinic Agonists, Nicotinic Antagonists, Spatial Memory, Electronic Nicotine Delivery Systems
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