Cited by 334Coffee research and impactsPhytochemicals and Antioxidant ActivitiesFood Chemistry and Fat Analysis

Comparative study of polyphenols and caffeine in different coffee varieties affected by the degree of roasting

Hečimović, Ivana, Belščak-Cvitanović, Ana, Horžić, Dunja, Komes, Draženka·2011·Food Chemistry

Abstract

The bioactive composition of coffee, as one of the most popular beverages in the world, has attracted interest as a potential source of beneficial bioactive compounds, especially polyphenols and caffeine. Since the content of these compounds is affected by the processing conditions, the objective of this study was to determine the content of polyphenolic compounds and caffeine in four different coffee varieties: Minas and Cioccolatato (Coffea arabica), and Cherry and Vietnam (Coffea canephora syn. Coffea robusta), roasted by three varying degrees (light, medium and dark). The content of the polyphenolic compounds and the antioxidant capacity of coffees were determined using UV/Vis spectrophotometric methods, while the content of chlorogenic acid derivatives was determined using HPLC analysis. The caffeine content was determined by means of two spectrophotometric methods, as well as HPLC analysis. Additionally, raw caffeine was also obtained by an isolation procedure with chloroform. Cherry coffee, a variety of C. canephora exhibited the highest overall content of total phenols (42.37mg GAE/g), followed by Minas coffee, while Cioccolatato contained the lowest TPC (33.12mg GAE/g). Cherry coffee also exhibited the highest content of individual classes of polyphenols (flavan-3-ols, procyanidins and tannins), while the highest content of chlorogenic acid (CQA) derivatives was determined in Minas and Cioccolatato coffees (C. arabica). The highest content of total and individual polyphenolic compounds was determined in coffees roasted in both light and medium roasting conditions, which was also observed for the content of CQA derivatives and antioxidant capacity of roasted coffees. The highest caffeine content in the coffee samples was determined by employing the HPLC analysis (0.06-2.55%). Light roasted Cherry coffee contained the highest overall content of caffeine among all coffees, which exhibited a decrease with intensified roasting.

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Crossref · 10.1016/j.foodchem.2011.05.059

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Authors
Hečimović, I., Belščak-Cvitanović, A., Horžić, D., & Komes, D.
Affiliations
University of Zagreb (HR)
Corresponding author
Komes, Draženka
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2011-12
Volume
129
Issue
3
Pages
991-1000
ISSN
0308-8146 (ISSN-L)
OpenAlex
W1980914868
Cited by
334
References
58
Citations by year
2026: 22 · 2025: 22 · 2024: 18 · 2023: 25 · 2022: 37 · 2021: 36 · 2020: 38 · 2019: 29 · 2018: 24 · 2017: 15 · 2016: 16 · 2015: 11 · 2014: 20 · 2013: 8 · 2012: 13
FWCI
9.60
Type
journal article
Language
en
Open access
closed
Locations
  • Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pharmacology › Coffee research and impacts
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food Chemistry and Fat Analysis
Keywords
coffee, polyphenols, caffeine, coffee roasting, Coffea arabica, Coffea canephora, chlorogenic acid derivatives, antioxidant capacity, flavan-3-ols, procyanidins, tannins, total phenolic content

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