Cited by 0Proteins in Food SystemsPolysaccharides Composition and ApplicationsFood composition and properties

Heat-induced gelation of ultrasonically extracted saline-alkali-grown soybean protein: Correlation between protein conformation and gel functional behavior

Han, Xinhui, Shan, Baosen, Li, Yajie, Liu, Jiaqi, Yang, Jing, Li, Dapeng·2027·Food Hydrocolloids

Verified 2026-11-06 · 5 references not checked yet

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Written from the paper’s full text.

TL;DR

This study compared four soybean samples—one saline-alkali-tolerant specialty variety (Qihuang 34, QP), two common varieties grown in saline-alkali soil (Jidou 23, Lindou 21), and one common variety grown in regular soil (Jiadou 33)—using alkali dissolution-acid precipitation (AD-AP) and ultrasound-assisted extraction (UAE). UAE improved protein recovery/content and heat-induced gel properties, with QP/U-QP showing the best gelation performance linked to higher 11S/7S ratio, disulfide bonds, and a more compact gel network.

Key findings

  • RSM optimization for QP gave optimal UAE conditions of 420 W, 20 min, and alkali extraction pH 9.36; measured protein concentration was 54.72 mg/mL versus predicted 55.00 mg/mL. The quadratic model had R² = 0.9877, adjusted R² = 0.9654, and lack-of-fit p = 0.1678.
  • UAE increased QP protein recovery from 56.00% to 70.33% (reported as an increase of 25.58%) and protein content from 89.56% to 91.61%. JP, LP, and AP recovery increases were 16.41%, 21.17%, and 12.09%, respectively; protein content increases were 1.78%, 1.02%, and 3.41%.
  • QP/U-QP showed the best gelation performance. Untreated QP, JP, and LP had disulfide bond contents of 9.46–9.65 μmol/g versus AP at 5.74 μmol/g; after UAE, disulfide contents increased by approximately 10%, 12%, 13%, and 11% for QP, JP, LP, and AP, with U-QP/U-JP/U-LP at 10.58–10.67 μmol/g versus U-AP at 6.30 μmol/g.
  • QP had the highest hydrophobic interaction and disulfide bond contents among non-ultrasonicated samples (approximately 4.00 and 5.68 mg/mL); U-QP increased these to approximately 5.02 and 6.78 mg/mL. WHC of QP increased by approximately 111% after ultrasound, compared with JP (~81%), LP (~86%), and AP (~81%); chewiness increases after ultrasound were approximately 10.4%, 13.6%, 9.7%, and 3.4% for QP, JP, LP, and AP.

Methods

  • Four soybean samples were used: Qihuang 34 (saline-alkali-tolerant specialty variety), Jidou 23 and Lindou 21 (common varieties grown in saline-alkali soil), and Jiadou 33 (common variety grown in regular soil). Proteins were extracted by AD-AP and UAE; UAE was optimized by Box-Behnken response surface methodology using Qihuang 34.
  • Structural characterization included SDS-PAGE, disulfide bond/sulfhydryl measurement, circular dichroism, fluorescence spectroscopy, and FTIR. Functional properties included solubility and water-holding capacity.
  • Heat-induced gels were prepared at 10% w/v protein, heated at 95 °C for 30 min, and stored at 4 °C for 12 h, then analyzed by texture profile analysis, SEM, intermolecular force fractionation, gel WHC, rheology, LF-NMR, and 7-day storage stability. Experiments were performed in triplicate with ANOVA and Pearson correlation analysis.

Limitations

  • The paper does not state a limitations section.
  • Evident from the text: UAE optimization was performed only on Qihuang 34, while the other varieties were compared under the same optimized conditions rather than individually optimized.
  • Evident from the text: gel evaluation used a single protein concentration (10% w/v) and fixed heating/storage conditions; storage stability was tested only up to 7 days at 4 °C, and the study reports structural correlations rather than direct causal evidence or application-level testing in foods.

Model-written summary. Check it against the paper before relying on it.

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Registry

Crossref · 10.1016/j.foodhyd.2026.113483

Cites

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Statistics

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Claims the paper makes

3 of 41 cited sources checked · 1 supported · 0 flagged · accuracy not yet measured

  • Premisecitation
    § 3.7.1. Gel properties · p. 12

    Disulfide bonds are capable of reinforcing covalent interprotein linkages and consequently contribute to the enhancement of gel network stability.

    “given that disulfide bonds are capable of reinforcing covalent interprotein linkages, they consequently contribute to the enhancement of gel network stability (Xin et al., 2025; Y. Zhao et al., 2024).”
    Quote in the paperSubstantial claim1 of 2 sources checked
    • cites Xin et al., 2025 source
    • cites Y. Zhao et al., 2024

      Couldn’t be matched to a paper in Crossref, so it can’t be checked.

  • Findingown data
    § A B S T R A C T · p. 1

    After ultrasonic treatment, the protein content of QP increased from 89.56% to 91.61%.

    “the protein content of QP increased from 89.56% to 91.61%”
    Quote in the paperNot a substantial claim
  • Findingreasoning
    § 3.4. Protein solubility · p. 8

    Following ultrasound treatment, the solubility of all four cultivars increased markedly.

    “Following ultrasound treatment, the solubility of all four culti­ vars increased markedly.”
    Quote in the paperSubstantial claim
  • Findingown data
    § 3.6.2. Disulfide bond contents · p. 11

    U-QP, U-JP, and U-LP remained significantly higher than U-AP (6.30 μmol/g).

    “and remained significantly higher than that of U-AP (6.30 μ mol/g).”
    Quote in the paperSubstantial claim
  • Findingown data
    § A B S T R A C T · p. 1

    After ultrasonic treatment, the protein recovery rate of QP increased from 56.00% to 70.33%.

    “the protein recovery rate increased from 56.00% to 70.33%”
    Quote in the paperNot a substantial claim
  • Classified as probably not a substantial claim. 9
  • May combine two claims. 73
  • The quote only partly states this claim. 1
  • Classified as probably not a substantial claim. May combine two claims.

    Premisecitation
    § 3.6.5. FTIR · p. 11

    The amide I region at 1600-1700 cm−1 is highly sensitive to changes in protein secondary structure and can be used to assess variations in α-helix, β-sheet, β-turn, and random coil structures.

    “Previous studies have also indicated that the amide I region at 1600-1700 cm 1 is highly sensitive to changes in protein secondary structure and can be used to assess variations in α -helix, β -sheet, β -turn, and random coil structures (L. Chen et al., 2019; Tong et al., 2022).”
    Quote in the paperSubstantial claim1 of 2 sources checked
    • cites L. Chen et al., 2019 source

      No passage in our indexed copy addresses the amide I region or its use to assess α-helix, β-sheet, β-turn, and random-coil structures. Searches for the wavenumber range, amide I, FTIR, and secondary structure returned no relevant passage; the only result for “structure” was a recent-citations entry, not evidence for this claim. (LLM, conf=0.71)

      Experimental — accuracy not yet measured
    • cites Tong et al., 2022 source
  • May combine two claims.

    Interpretationreasoning
    § 3.9. Storage stability · p. 16

    UAE enhanced the elasticity and structural stability of saline-alkali soybean protein gels.

    “indicating that UAE enhanced the elasticity and structural stability of saline-alkali soybean protein gels (Barekat & Ubeyitogullari, 2025; Ren et al., 2024).”
    Quote in the paperSubstantial claim1 of 2 sources checked
    • cites Barekat & Ubeyitogullari, 2025 source

      Section 2.4 describes sonication during preparation of a soy protein isolate gel, and Section 3.6 discusses the shape stability of a printed sorghum–soy gel. Neither passage says that UAE enhanced elasticity or structural stability in saline-alkali soybean protein gels; searches for UAE, saline-alkali gels, and an ultrasound-treatment comparison found no such result. (LLM, conf=0.76)

      Experimental — accuracy not yet measured
    • cites Ren et al., 2024 source
  • May combine two claims.

    Findingown data
    § 3.6.1. SDS-PAGE · p. 9

    The band positions were generally consistent among all samples, and no obvious new or missing bands were observed, indicating that neither soybean source nor extraction method altered the major protein subunit composition.

    “The band positions were generally consistent among all samples, and no obvious new or missing bands were observed, indicating that neither soybean source nor extraction method altered the major protein subunit composition.”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § + 0 . 8347 C -0 . 9604 AB -2 . 40 AC -0 . 8413 BC -5 . 19 A 2 -0 . 9522 B 2 -0 . 8290 C 2 · p. 6

    Among all influencing factors, the quadratic term of ultrasonic power (A2) had the most significant effect on protein concentration, followed by the linear term of alkali dissolution pH (C) and the quadratic term of ultrasonic time (B2).

    “Among all influencing factors, the quadratic term of ultrasonic power (A 2 ) had the most significant effect on protein concentration, followed by the linear term of alkali dissolution pH (C) and the quadratic term of ultrasonic time (B 2 ).”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingcitation
    § 3.5. Protein water-holding capacity (WHC) · p. 8

    The WHC of samples subjected to UAE treatment was markedly greater than that of those obtained via the AD-AP procedure, and the WHC of QP increased by approximately 111%, higher than JP (≈81%), LP (≈86%), and AP (≈81%).

    “In general, the WHC of samples subjected to UAE treatment was markedly greater than that of those obtained via the AD-AP procedure (Fig. 2B)”
    • Also stated in: § 3.5. Protein water-holding capacity (WHC) · p. 9 · plain
      “Specifically, the WHC of QP increased by approximately 111% after ultrasonic treatment, which was higher than that of JP ( ≈ 81%), LP ( ≈ 86%), and AP ( ≈ 81%).”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.6.5. FTIR · p. 11

    All samples exhibited typical mid-infrared FTIR absorption bands of soy protein, including the amide I band at 1658.91 cm−1, the amide II band at 1528.00 cm−1, and the amide III band at 1237.82 cm−1.

    “All samples exhibited typical mid-infrared FTIR absorption bands of soy protein, including the amide I band at 1658.91 cm 1 , the amide II band at 1528.00 cm 1 , and the amide III band at 1237.82 cm 1”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § 3.6.2. Disulfide bond contents · p. 11

    The disulfide bond contents of QP, JP, LP, and AP increased by approximately 10%, 12%, 13%, and 11%, respectively, after ultrasound-assisted extraction.

    “the disulfide bond contents of QP, JP, LP, and AP increased by approximately 10%, 12%, 13%, and 11%, respectively.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.6.3. Circular dichroism (CD) spectra · p. 11

    QP showed the strongest CD signal and AP the weakest.

    “with QP showing the strongest CD signal and AP the weakest”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § 3.6.3. Circular dichroism (CD) spectra · p. 11

    Both the CD spectra and secondary-structure analysis showed a slight decrease in α-helix content and a slight increase in β-sheet content after sonication.

    “both the CD spectra and secondary-structure analysis showed a slight decrease in α -helix content and a slight increase in β -sheet content after sonication”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § 3.6.1. SDS-PAGE · p. 9

    After UAE treatment, the 11S/7S ratio increased in QP, LP, and AP, with U-QP exhibiting the highest value, followed by U-LP, indicating a relatively higher proportion of 11S in these samples.

    “After UAE treat­ ment, the 11S/7S ratio increased in QP, LP, and AP, with U-QP exhib­ iting the highest value, followed by U-LP, indicating a relatively higher proportion of 11S in these samples.”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § + 0 . 8347 C -0 . 9604 AB -2 . 40 AC -0 . 8413 BC -5 . 19 A 2 -0 . 9522 B 2 -0 . 8290 C 2 · p. 6

    The lack-of-fit assessment yielded a nonsignificant result (p = 0.1678), from which it was inferred that the model provided an adequate description of the data.

    “The lack-of-fit assessment yielded a nonsignificant result (p = 0.1678), from which it was inferred that the model provided an adequate description of the data.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.7.2. Scanning Electron Microscopy · p. 13

    AP displayed the least uniform structure, with larger pores, rough pore walls, and obvious lamellar stacking.

    “AP displayed the least uniform structure, with larger pores, rough pore walls, and obvious lamellar stacking.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.6.5. FTIR · p. 11

    Ultrasound-assisted extraction induced slight blue shifts and changes in peak shape.

    “but it induced slight blue shifts and changes in peak shape”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § 3.7.3. Intermolecular forces · p. 13

    Although the disulfide bond content of U-AP increased to approximately 4.63 mg/mL, its hydrophobic interaction content remained relatively low, while its ionic bond content increased more noticeably.

    “Although the disulfide bond content of U-AP increased to approximately 4.63 mg/mL, its hydrophobic interaction content remained relatively low, while its ionic bond content increased more noticeably.”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § 3.9. Storage stability · p. 15

    Compared with AP, soybean proteins derived from saline-alkali soil, including QP, JP, and LP, exhibited better shape retention, with QP and LP showing more complete gel structures.

    “Compared with AP, soybean proteins derived from saline-alkali soil, including QP, JP, and LP, exhibited better shape retention, with QP and LP showing more complete gel structures.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.9. Storage stability · p. 15

    In strain sweep, G′ and G′′ remained relatively stable within the low-strain region, whereas both moduli decreased rapidly with increasing strain.

    “G ′ and G ″ remained relatively stable within the low-strain region, whereas both moduli decreased rapidly with increasing strain”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.7.2. Scanning Electron Microscopy · p. 12

    All samples were able to form gel structures and maintain their basic shape when inverted.

    “all samples were able to form gel structures and maintain their basic shape when inverted”
    Quote in the paperSubstantial claim
  • The quote only partly states this claim.

    Findingown data
    § 3.7.3. Intermolecular forces · p. 13

    LP and JP showed intermediate levels of hydrophobic interaction and disulfide bond contents.

    “LP and JP showed intermediate levels”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § 3.7.2. Scanning Electron Microscopy · p. 13

    Within the UAE group, QP exhibited the most compact and homogeneous network, with fine interconnected pores and an intact cross-linked framework.

    “Within the UAE group, QP exhibited the most compact and homogeneous network, with fine interconnected pores and an intact cross-linked framework.”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § 3.9. Storage stability · p. 15

    At a protein concentration of 10%, all samples formed relatively stable gels on day 0 and maintained their basic shape when placed at an inclined angle.

    “at a protein concentration of 10%, all samples formed relatively stable gels on day 0 and maintained their basic shape when placed at an inclined angle.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.7.3. Intermolecular forces · p. 13

    AP had the lowest hydrophobic interaction and disulfide bond contents, approximately 1.75 and 3.57 mg/mL, respectively.

    “whereas AP had the lowest hydrophobic interaction and disulfide bond contents, approximately 1.75 and 3.57 mg/mL, respectively.”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingnone given
    § 3.7.2. Scanning Electron Microscopy · p. 13

    JP presented slightly enlarged local pores and reduced network continuity.

    “whereas JP presented slightly enlarged local pores and reduced network continuity.”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Findingown data
    § 3.7.5. Rheological properties · p. 14

    In the frequency sweep, both G′ and G′′ increased with increasing frequency, and G′ was always higher than G′′.

    “In the frequency sweep (Fig. 9C), both G ′ and G ʹ ʹ increased with increasing frequency, and G ʹ was always higher than G ʹ ʹ”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.7.2. Scanning Electron Microscopy · p. 12

    The AP gel showed irregular edges and obvious local collapse.

    “The AP gel showed irregular edges and obvious local collapse”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.7.2. Scanning Electron Microscopy · p. 13

    At 100× magnification, the AD-AP samples exhibited a relatively rough network structure, with larger and irregularly distributed pores, accompanied by local fractures, collapse, or lamellar aggregation.

    “At 100 × magnification, the AD-AP samples exhibited a relatively rough network structure, with larger and irregularly distributed pores, accompanied by local fractures, collapse, or lamellar aggregation”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingreasoning
    § 3.7.5. Rheological properties · p. 14

    U-QP showed a markedly lower maximum creep compliance (Jmax) and more complete strain recovery during the recovery phase.

    “U-QP showing a markedly lower maximum creep compliance (Jmax) and more complete strain recovery during the recovery phase”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingreasoning
    § 3.7.2. Scanning Electron Microscopy · p. 13

    After ultrasound treatment, the integrity and uniformity of all gels were improved, and edge collapse was reduced, particularly in U-QP and U-LP.

    “After ultrasound treatment, the integrity and uniformity of all gels were improved, and edge collapse was reduced, particularly in U-QP and U-LP”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.7.1. Gel properties · p. 12

    Gumminess and chewiness were further improved after ultrasound treatment, with U-QP showing relatively superior performance.

    “and were further improved after ultrasound treatment, with U-QP showing relatively superior performance.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingreasoning
    § 3.7.2. Scanning Electron Microscopy · p. 13

    UAE-treated samples formed a more uniform, continuous, and compact three-dimensional network, characterized by smaller and more regularly distributed pores as well as a more intact gel skeleton.

    “the UAE-treated samples formed a more uniform, continuous, and compact threedimensional network, characterized by smaller and more regularly distributed pores as well as a more intact gel skeleton.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.10. Correlation analysis · p. 16

    Ionic bonds were negatively correlated with most textural and rheological parameters, while hydrophobic interactions were positively associated with G′ and G′′.

    “Regarding inter­ molecular interactions, ionic bonds were negatively correlated with most textural and rheological parameters, while hydrophobic in­ teractions were positively associated with G ′ and G ″ .”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.10. Correlation analysis · p. 16

    The 11S/7S ratio was significantly positively correlated with β-sheet and S-S contents, as well as with hardness, fracturability, gumminess, chewiness, firmness, WHC, storage modulus (G′), and loss modulus (G′′), while it was negatively correlated with α-helix content and Jmax.

    “The 11S/7S ratio was significantly positively correlated with β -sheet and S-S contents, as well as with hardness, fracturability, gumminess, chewiness, firmness, WHC, storage modulus (G ′ ), and loss modulus (G ″ ), while it was negatively correlated with α -helix content and Jmax.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingreasoning
    § 3.9. Storage stability · p. 16

    U-QP exhibited the lowest creep deformation and the greatest recovery.

    “In contrast, U-QP exhibited the lowest creep deformation and the greatest recovery”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.9. Storage stability · p. 16

    AP had the highest shear strain and residual strain.

    “The creep-recovery results showed that AP had the highest shear strain and residual strain”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingreasoning
    § 4. Conclusion · p. 16

    Compared with AD-AP, UAE significantly improved protein extraction yield and enhanced water-holding capacity and gel properties, particularly for soybean proteins from soybeans cultivated in saline-alkali soil.

    “Compared with AD-AP, UAE significantly improved protein extraction yield and enhanced water-holding capacity and gel proper­ ties, particularly for soybean proteins from soybeans cultivated in salinealkali soil.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingreasoning
    § 4. Conclusion · p. 16

    Consequently, the gels exhibited higher hardness, greater water-holding capacity, stronger elastic behavior, and improved storage stability.

    “Consequently, the gels exhibited higher hardness, greater waterholding capacity, stronger elastic behavior, and improved storage sta­ bility.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.10. Correlation analysis · p. 16

    β-sheet and S-S contents showed significant positive correlations with most textural parameters, WHC, G′, and G′′, whereas α-helix and β-turn contents exhibited significant negative correlations with these gel properties.

    “In particular, β -sheet and S-S contents showed significant positive correlations with most textural parameters, WHC, G ′ , and G ″ , whereas α -helix and β -turn contents exhibited signif­ icant negative correlations with these gel properties.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Findingown data
    § 3.10. Correlation analysis · p. 16

    Strong positive correlations were observed among textural properties, WHC, G′, and G′′, whereas Jmax was negatively correlated with most of these parameters.

    “Moreover, strong positive correlations were observed among textural properties, WHC, G ′ , and G ″ , whereas Jmax was negatively correlated with most of these pa­ rameters.”
    Quote in the paperSubstantial claim
  • Classified as probably not a substantial claim. May combine two claims.

    Study design and conductnone given
    § 2.11. Data analysis · p. 6

    All experiments were independently performed in triplicate, and the resulting data are reported as the mean ± standard deviation.

    “All experiments were independently performed in triplicate, and the resulting data are reported as the mean ± standard deviation.”
    Quote in the paperSubstantial claim
  • Probably not a substantial claim: may not pass S3 (load-bearing). May combine two claims.

    Premisecitation
    § 3.6.3. Circular dichroism (CD) spectra · p. 11

    Byanju et al. reported a decrease in α-helix and an increase in β-structure after ultrasound treatment, which may be related to acoustic cavitation-induced perturbation of hydrogen bonding and electrostatic interactions.

    “Byanju et al. also reported a decrease in α -helix and an increase in β -structure after ultrasound treatment, which may be related to acoustic cavitation-induced perturbation of hydrogen bonding and electrostatic interactions (Byanju et al., 2020).”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites Byanju et al., 2020 source
  • Classified as probably not a substantial claim. May combine two claims.

    Premisecitation
    § 3.6.4. Fluorescence spectroscopy · p. 11

    The polarity of the local environment near hydrophobic side chains is commonly monitored by fluorescence spectroscopy, which can reveal alterations in the three-dimensional conformation of proteins.

    “The polarity of the local environment near hydrophobic side chains is commonly monitored by fluorescence spectroscopy, which can reveal alterations in the three-dimensional conformation of proteins (Z. Ma et al., 2022).”
    Quote in the paperSubstantial claim0 of 1 sources checked
    • cites Z. Ma et al., 2022

      Couldn’t be matched to a paper in Crossref, so it can’t be checked.

  • May combine two claims.

    Interpretationown data
    § 3.6.4. Fluorescence spectroscopy · p. 11

    Proteins from soybeans grown in saline-alkali soils may possess a looser tertiary structure, allowing aromatic amino acid residues to become more exposed to the surrounding environment.

    “This suggests that proteins from soybeans grown in saline-alkali soils may possess a looser tertiary structure, allowing aromatic amino acid residues to become more exposed to the surrounding environment.”
    Quote in the paperNot a substantial claim
  • May combine two claims.

    Interpretationreasoning
    § A B S T R A C T · p. 1

    The synergistic contribution of the ultrasonic-induced changes facilitated protein aggregation and cross-linking, resulting in a more compact and continuous three-dimensional gel network and consequently improving the gel properties.

    “The synergistic contribution of these changes facilitated protein aggregation and cross-linking, resulting in the formation of a more compact and continuous three-dimensional gel network and consequently improving the gel properties.”
    Quote in the paperSubstantial claim
  • Classified as probably not a substantial claim. May combine two claims.

    Premisecitation
    § 3.6.3. Circular dichroism (CD) spectra · p. 11

    The positive band at approximately 190-195 nm and the negative band near 208 nm are associated with β-sheet and α-helical structures, respectively.

    “Tan et al. reported that the positive band at approximately 190-195 nm and the negative band near 208 nm are associated with β -sheet and α -helical structures, respectively (Tan et al., 2024).”
    Quote in the paperSubstantial claim0 of 1 sources checked
    • cites Tan et al., 2024 source
  • May combine two claims.

    Interpretationown data
    § 3.1.3. Validation of the predictive model · p. 7

    Under the optimal extraction parameters of 420 W sonication power, 20 min treatment duration, and pH 9.36, the experimentally measured protein concentration was 54.72 mg/mL, close to the predicted 55.00 mg/mL, confirming the validity of the model.

    “Under these conditions, the experimentally measured protein concentration was 54.72 mg/mL, which was close to the pre­ dicted value of 55.00 mg/mL, thereby confirming the validity of the model.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Premisecitation
    § A B S T R A C T · p. 2

    The traditional alkali dissolution-acid precipitation method suffers from low extraction efficiency, severe protein aggregation, and poor functional properties.

    “However, it suffers from low extraction efficiency, severe protein aggregation, and poor functional properties (Chandran et al., 2024; Luthria et al., 2018; Y. Zhao et al., 2022).”
    Quote in the paperSubstantial claim0 of 3 sources checked
    • cites Chandran et al., 2024

      Couldn’t be matched to a paper in Crossref, so it can’t be checked.

    • cites Luthria et al., 2018 source
    • cites Y. Zhao et al., 2022 source

      Checked before — its earlier verdict is held back until a new check runs.

  • May combine two claims.

    Interpretationreasoning
    § 3.7.3. Intermolecular forces · p. 14

    This may explain the improved gel strength, textural stability, and water-holding capacity of saline-alkali soybean proteins, particularly U-QP.

    “which may explain the improved gel strength, textural stability, and water-holding capacity of saline-alkali soybean proteins, particularly U-QP.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationown data
    § 3.8. Low-field nuclear magnetic resonance · p. 15

    QP, JP, and LP, particularly after UAE treatment, were more prone to forming compact and stable gel networks, thereby enhancing water immobilization.

    “Overall, QP, JP, and LP, particularly after UAE treatment, were more prone to forming compact and stable gel networks, thereby enhancing water immobilization.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationown data
    § 3.7.5. Rheological properties · p. 14

    QP and some other samples had denser gel networks and stronger structural integrity.

    “indicating denser gel networks and stronger structural integrity (Guannan Liu, Jin, et al., 2025; Ren et al., 2024; Sun et al., 2025; Zou et al., 2026).”
    Quote in the paperSubstantial claim0 of 3 sources checked
    • cites Guannan Liu, Jin, et al., 2025

      Couldn’t be matched to a paper in Crossref, so it can’t be checked.

    • cites Ren et al., 2024 source
    • cites Sun et al., 2025 source
  • May combine two claims.

    Interpretationcitation
    § 3.9. Storage stability · p. 15

    UAE enhanced gel storage stability by promoting moderate protein unfolding, strengthening intermolecular cross-linking, and restricting water migration.

    “suggesting that UAE enhanced gel storage stability by promoting moderate protein unfold­ ing, strengthening intermolecular cross-linking, and restricting water migration (Qiu et al., 2025; Zou et al., 2026).”
    Quote in the paperSubstantial claim0 of 2 sources checked
    • cites Qiu et al., 2025 source
    • cites Zou et al., 2026 source
  • May combine two claims.

    Premisecitation
    § 3.8. Low-field nuclear magnetic resonance · p. 15

    T2 relaxation time in LF-NMR can effectively reflect the water state and molecular mobility in gel systems; a shorter T2 relaxation time generally indicates stronger restriction of water molecules by the gel network and reduced water mobility.

    “Previous studies have demonstrated that T2 relaxation time in LF-NMR can effectively reflect the water state and molecular mobility in gel systems; a shorter T2 relaxation time generally indicates stronger restriction of water molecules by the gel network and reduced water mobility (Ren et al., 2025; Xin et al., 2025; Y. Zhao et al., 2024).”
    Quote in the paperSubstantial claim0 of 3 sources checked
    • cites Ren et al., 2025 source
    • cites Xin et al., 2025 source

      Checked before — its earlier verdict is held back until a new check runs.

    • cites Y. Zhao et al., 2024

      Couldn’t be matched to a paper in Crossref, so it can’t be checked.

  • May combine two claims.

    Interpretationown data
    § 3.7.5. Rheological properties · p. 14

    QP and U-QP combined a relatively high 11S/7S ratio with higher disulfide-bond content.

    “QP and U-QP combined a relatively high 11S/7S ratio with higher disulfide-bond content”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationcitation
    § 3.7.3. Intermolecular forces · p. 14

    The increased availability of these reactive sites strengthens hydrophobic association and promotes the formation of disulfide linkages, thereby contributing to a more organized and densely cross-linked gel network.

    “The increased availability of these reactive sites strengthens hydrophobic association and promotes the formation of disulfide linkages, thereby contributing to a more organized and densely cross-linked gel network (Teng et al., 2026).”
    Quote in the paperSubstantial claim0 of 1 sources checked
    • cites Teng et al., 2026 source
  • May combine two claims.

    Interpretationreasoning
    § 3.6.5. FTIR · p. 11

    This moderate degree of unfolding could enhance the exposure of active groups and improve intermolecular interactions.

    “This moderate degree of unfolding could enhance exposure of active groups and improve intermolecular interactions.”
    Quote in the paperSubstantial claim
  • Classified as probably not a substantial claim. May combine two claims.

    Premisecitation
    § 3.6.5. FTIR · p. 11

    Tong et al. reported that ultrasound treatment promoted the structural unfolding of soy protein and exposed hydrophobic regions.

    “Tong et al. further reported that ultrasound treatment promoted the structural unfolding of soy protein and exposed hydrophobic regions (Z. Ma et al., 2022; Tong et al., 2022).”
    Quote in the paperSubstantial claim0 of 2 sources checked
    • cites Z. Ma et al., 2022

      Couldn’t be matched to a paper in Crossref, so it can’t be checked.

    • cites Tong et al., 2022 source
  • May combine two claims.

    Interpretationreasoning
    § 3.7.5. Rheological properties · p. 15

    This coincided with higher gel hardness, higher elastic modulus, a denser SEM network, and stronger water immobilization.

    “which coincided with higher gel hardness, higher elastic modulus, a denser SEM network, and stronger water immobilization.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationreasoning
    § 3.7.3. Intermolecular forces · p. 13

    Ionic and hydrogen bonds were more closely associated with water retention and the improvement of network uniformity.

    “By contrast, ionic and hydrogen bonds were more closely associated with water retention and the improvement of network uniformity.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationreasoning
    § 3.8. Low-field nuclear magnetic resonance · p. 15

    A larger proportion of free water was trapped inside the gel matrix and subsequently transformed into a less mobile state.

    “and that a larger proportion of free water was trapped inside the gel matrix and subsequently transformed into a less mobile state.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationown data
    § 3.7.5. Rheological properties · p. 14

    The gel systems were dominated by elastic responses and had formed relatively stable three-dimensional network structures.

    “indicating that the gel systems were dominated by elastic responses and had formed relatively stable three-dimensional network structures.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationown data
    § 3.9. Storage stability · p. 15

    The gel systems were dominated by elastic behavior and had formed three-dimensional network structures.

    “suggesting that the gel systems were dominated by elastic behavior and had formed three-dimensional network struc­ tures (Fig. S4B -C).”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationown data
    § 3.8. Low-field nuclear magnetic resonance · p. 15

    AP and U-AP gels had a relatively loose network structure and weaker restriction on water molecules.

    “indicating a relatively loose network structure and weaker restriction on water molecules.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationreasoning
    § 3.7.5. Rheological properties · p. 14

    Compared with AP, QP showed greater structural integrity and resistance to deformation.

    “Compared with AP, QP showed greater structural integrity and resistance to deformation.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationnone given
    § 3.8. Low-field nuclear magnetic resonance · p. 15

    The peak appearing at a shorter relaxation time was assigned to bound water (T21), which was more strongly associated with the protein network.

    “In contrast, the peak appearing at a shorter relaxation time was assigned to bound water (T21), which was more strongly associated with the protein network.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationreasoning
    § 3.7.5. Rheological properties · p. 14

    Both the intrinsic varietal characteristics of saline-alkali soybean proteins and UAE treatment contributed to improved gel elasticity, deformation resistance, and structural stability, with U-QP showing the best overall performance.

    “Therefore, both the intrinsic varietal characteristics of saline-alkali soybean proteins and UAE treatment contributed to improved gel elasticity, deformation resistance, and structural stability, with U-QP showing the best overall performance.”
    Quote in the paperSubstantial claim
  • Classified as probably not a substantial claim. May combine two claims.

    Premisecitation
    § 3.7.3. Intermolecular forces · p. 14

    Yang and co-workers revealed that ultrasound treatment could alter the proportional roles of ionic bonds, hydrogen bonds, hydrophobic forces, and disulfide crosslinks within soy protein gels, and that such changes were dependent on the pH environment.

    “the study by Yang and co-workers revealed that ultrasound treatment could alter the proportional roles of ionic bonds, hydrogen bonds, hydrophobic forces, and disulfide crosslinks within soy protein gels, and that such changes were dependent on the pH environment (J. Yang et al., 2023).”
    Quote in the paperSubstantial claim0 of 1 sources checked
    • cites J. Yang et al., 2023 source

      Checked before — its earlier verdict is held back until a new check runs.

  • May combine two claims.

    Interpretationreasoning
    § 3.7.2. Scanning Electron Microscopy · p. 13

    Ultrasound treatment promoted moderate protein unfolding and enhanced intermolecular interactions, thereby improving the gel network structure.

    “These results indicate that ultrasound treatment promoted moderate protein unfolding and enhanced intermolecular interactions, thereby improving the gel network structure”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationown data
    § 3.9. Storage stability · p. 15

    QP's higher G′ indicates a denser gel network and stronger structural support.

    “indicating a denser gel network and stronger structural support”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationown data
    § 3.7.5. Rheological properties · p. 14

    QP had a denser gel network and stronger elastic response.

    “indicating a denser gel network and stronger elastic response.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationown data
    § 3.7.2. Scanning Electron Microscopy · p. 13

    AD-AP samples had poor network continuity and compactness.

    “indicating poor network continuity and compactness.”
    Quote in the paperNot a substantial claim
  • Probably not a substantial claim: may not pass S3 (load-bearing).

    Premisecitation
    § 3.7.3. Intermolecular forces · p. 13

    Yang and colleagues reported that disulfide linkages constituted the major intermolecular force controlling the formation of thermally induced gels from soy protein hydrolysates.

    “Yang and colleagues reported that disulfide linkages constituted the major intermolecular force controlling the formation of thermally induced gels from soy protein hydrolysates (J. Yang et al., 2023)”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites J. Yang et al., 2023 source

      Checked before — its earlier verdict is held back until a new check runs.

  • May combine two claims.

    Interpretationreasoning
    § 3.7.3. Intermolecular forces · p. 14

    Ultrasound treatment further strengthened protein-protein cross-linking and improved gel network formation.

    “whereas ultrasound treatment further strengthened protein-protein cross-linking and improved gel network formation.”
    Quote in the paperSubstantial claim
  • Probably not a substantial claim: may not pass S3 (load-bearing). May combine two claims.

    Premisecitation
    § 3.7.4. Water-holding capacity · p. 14

    Ren et al. demonstrated that the water-fixation ability of gel systems is closely associated with network integrity and intermolecular interactions, and that a stable gel network can reduce water migration and release.

    “Ren et al. also demonstrated that the water-fixation ability of gel systems is closely associated with network integrity and intermolecular interactions, and that a stable gel network can reduce water migration and release (Ren et al., 2024).”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites Ren et al., 2024 source
  • May combine two claims.

    Interpretationreasoning
    § 3.7.5. Rheological properties · p. 14

    This result was consistent with the higher storage modulus and elasticity-dominated behavior observed in the amplitude, time, and frequency sweep tests, pointing to the establishment of a tighter and improved gel architecture in the U-QP system.

    “This result was consistent with the higher storage modulus and elasticity-dominated behavior observed in the amplitude, time, and frequency sweep tests, pointing to the establishment of a tighter and improved gel architecture in the U-QP system.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationreasoning
    § 4. Conclusion · p. 16

    Ultrasonic treatment induced conformational rearrangement of the protein chains, promoting the exposure of thiol groups and aromatic side chains and thereby facilitating disulfide crosslinking, hydrophobic interactions, and hydrogen bonding under thermal conditions.

    “Second, ultrasonic treatment induced conformational rearrangement of the protein chains, promoting the exposure of thiol groups and aromatic side chains and thereby facilitating disulfide crosslinking, hydrophobic interactions, and hydrogen bonding under thermal conditions.”
    Quote in the paperSubstantial claim
  • May combine two claims.

    Interpretationcitation
    § 3.9. Storage stability · p. 16

    The observed enhancement can be ascribed to moderate protein unfolding brought about by ultrasonic treatment, which increases the accessibility of nonpolar and thiol moieties, reinforces hydrophobic associations and disulfide bridging, and thus improves the viscoelastic stability of gels under chilled conditions.

    “The observed enhancement can be ascribed to mod­ erate protein unfolding brought about by ultrasonic treatment, which increases the accessibility of nonpolar and thiol moieties, reinforces hydrophobic associations and disulfide bridging, and thus improves the viscoelastic stability of gels under chilled conditions.”
    Quote in the paperSubstantial claim0 of 1 sources checked
    • cites Qiu et al., 2025 source
  • May combine two claims.

    Interpretationown data
    § 3.9. Storage stability · p. 16

    AP had poorer resistance to deformation and weaker recovery ability.

    “indicating poorer resistance to deformation and weaker recovery ability (Fig. S4D).”
    Quote in the paperSubstantial claim
75 of 75
  • The paper relies on this without support.

    Studies on the extraction characteristics and gelation behavior of soybean proteins derived from saline-alkali environments remain scarce.

    “However, studies on the extraction characteristics and gelation behavior of soybean proteins derived from saline-alkali environments remain scarce.”
  • The paper relies on this without support.

    The difference in protein concentration between sequentially applied dissociation solutions specifically reflects the contribution of ionic interactions, hydrogen bonding, hydrophobic interactions, and disulfide bonds, respectively.

    “Specifically, the difference between S2 and S1 was used to indicate the contribution of ionic interactions, while S3-S2 reflected the involvement of hydrogen bonding. Similarly, the contri­ bution of hydrophobic interactions was estimated from the difference between S4 and S3, and that of disulfide bonds was determined based on S5- S4.”
  • The paper relies on this without support.

    The BCA protein assay with blank correction accurately quantifies protein concentration in the supernatants despite the presence of dissociation agents.

    “In order to miti­ gate the potential interference induced by the dissociation agents, the corresponding dissociation solutions were used as blank controls for correction.”
  • The paper relies on this without support.

    SDS-PAGE band intensity reflects the relative contents of 7S and 11S fractions.

    “However, differences in band intensity suggested varia­ tions in the relative contents of 7S and 11S fractions”
  • The paper relies on this without support.

    Hardness is an important indicator of gel strength, and a higher hardness value reflects a denser and more stable protein gel structure.

    “Hardness is generally regarded as an important indicator of gel strength, and a higher hardness value reflects a denser and more stable protein gel structure.”
  • The paper relies on this without support.

    Changes in intermolecular interactions are major determinants of the physicochemical and structural behavior of protein gels.

    “Changes in intermolecular interactions are generally considered to be major determinants of the physicochemical and structural behavior of protein gels.”
  • The paper relies on this without support.

    Ionic and hydrogen bonds mainly regulate protein-water contact, while hydrophobic associations and disulfide linkages play a dominant role in mediating protein-protein interactions.

    “Among these forces, ionic and hydrogen bonds mainly regulate the protein-water contact, while hydrophobic associations and disulfide linkages play a dominant role in mediating protein-protein interactions.”
7 of 7

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

Each contradiction between two passages listed here was confirmed by an independent check.

  • Reported significance ranking conflicts with the ANOVA table

    Minor

    The text ranks pH (C) and ultrasonic time squared (B2) immediately after A2 among all terms. Table 2 shows that linear ultrasonic power (A) has a larger F-value and smaller p-value than either term.

1 of 1

Cites

Flagged first, then by citations

Research works printed but not linked: 2

  1. Dang, 2023, Structural, functional properties of protein and characteristics of tofu from small-seeded soybeans grown in the Loess Plateau of China, Food Chemistry X, 18, 10.1016/j.fochx.2023.100689 · DOI
  2. Wang, 2026, Mechanistic insights into pH-dependent structural and performance properties of diacylglycerol based high internal phase pickering emulsion-myofibrillar protein composite gels, Food Chemistry X, 36, 10.1016/j.fochx.2026.103896 · DOI

Cited by

Still being checked.

Authors
Han, X., Shan, B., Li, Y., Liu, J., Yang, J., & Li, D.
Affiliations
Shandong Agricultural University (CN)
Corresponding author
  • Li, Yajie
  • Yang, Jing
  • Li, Dapeng
Venue
Food Hydrocolloids
Publisher
Elsevier BV
Published
2027-04
Volume
185
Pages
113483
ISSN
0268-005X (ISSN-L)
OpenAlex
W7220677348
Cited by
0
References
69
FWCI
0.00
Type
journal article
Language
en
Open access
closed
Locations
Food Hydrocolloids (publishedVersion)
Funders
Key Technology Research and Development Program of Shandong Province (2024SFGC0401)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
Keywords
soy protein, heat-induced gelation, saline-alkali soil, ultrasound-assisted extraction, protein conformation, disulfide bonds, gel microstructure, rheological properties, protein recovery, plant protein gels, functional foods

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