Effect of Antioxidant-Enriched Diets on Glutathione Redox Status in Tissue Homogenates and Mitochondria of the Senescence-Accelerated Mouse
Open accessAbstract
The main purpose of this study was to investigate whether consumption of diets enriched in antioxidants attenuates the level of oxidative stress in the senescence-accelerated mouse (SAM). In separate and independent studies, two different dietary mixtures, one enriched with vitamin E, vitamin C, L-carnitine, and lipoic acid (Diet I) and another diet including vitamins E and C and 13 additional ingredients containing micronutrients with bioflavonoids, polyphenols, and carotenoids (Diet II), were fed for 8 and 10 months, respectively. The amounts of glutathione (GSH) and glutathione disulfides (GSSG) and GSH:GSSG ratios were determined in plasma, tissue homogenates, and mitochondria isolated from five different tissues of SAM (P8) mice. Both diets had a reductive effect in plasma; however Diet I had relatively little effect on the glutathione redox status in tissue homogenates or mitochondria. Remarkably, Diet II caused a large increase in the amount of glutathione and a marked reductive shift in glutathione redox state in mitochondria. Overall, the effects of Diet II were tissue and gender specific. Results indicated that the glutathione redox state in mitochondria and tissues can be altered by supplemental intake of a relatively complex mixture of dietary antioxidants that contains substances known to induce phase 2 enzymes, glutathione, and antioxidant defenses. Whether corresponding attenuations occur in age-associated deleterious changes in physiological functions or life span remains unknown.
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Rebrin et al. demonstrated that benefits of diets enriched in vitamins and micronutrients should be ascribed to increased plasma levels of GSH and improved mitochondrial redox homeostasis in a sex- and tissue-dependent manner.
“In this respect, Rebrin et al. demonstrated that the benefits of diets enriched in vitamins and micronutrients should be ascribed to increased plasma levels of GSH and improved mitochondria redox homeostasis in a sex and tissue dependent manner [197].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [197]
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Retracted
- Authors
- Rebrin, I., Zicker, S., Wedekind, K. J., Paetau-Robinson, I., Packer, L., & Sohal, R. S.
- Affiliations
- University of Southern California (US)
- Hill's Pet Nutrition (United States) (US)
- Corresponding author
- Sohal, Rajindar S.
- ORCID
- Wedekind, Karen J.
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2005-08
- Volume
- 39
- Issue
- 4
- Pages
- 549-557
- ISSN
- 0891-5849 (ISSN-L)
- PMC
- PMC2837083
- OpenAlex
- W2047056169
- Cited by
- 82
- References
- 56
- Citations by year
- 2026: 1 · 2025: 4 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 5 · 2019: 5 · 2018: 2 · 2016: 5 · 2015: 5 · 2014: 4 · 2013: 4 · 2012: 10
- FWCI
- 2.95
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (R01 AG13563, R01AG013563)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- glutathione redox state, glutathione disulfide, senescence-accelerated mice, oxidative stress, mitochondria, vitamin E, vitamin C, alpha-lipoic acid, L-carnitine, polyphenols, carotenoids, bioflavonoids, phase II enzymes, aging, tissue-specific effects, sex differences
- MeSH terms
- Aging, Animals, Antioxidants, Brain, Chromatography, High Pressure Liquid, Cysteine, Diet, Female, Glutathione, Kidney, Liver, Male, Methionine, Mitochondria, Myocardium, Oxidation-Reduction, Muscle, Skeletal, Glutathione Disulfide, Mice
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