Quantitative proteomics reveals distinct composition of amyloid plaques in Alzheimer’s disease
Abstract
INTRODUCTION: We investigated the proteomic profiles of amyloid plaques (APs) from Alzheimer's disease (AD) and age-matched non-AD brains and APP/PS1 transgenic model mice. METHODS: APs and adjacent control regions were collected from fresh-frozen brain sections using laser capture dissection. Proteins were quantitated using tag-labeling coupled high-throughput mass spectra. RESULTS: Over 4000 proteins were accurately quantified, and more than 40 were identified as highly enriched in both AD and non-AD APs, including apoE, midkine, VGFR1, and complement C4. Intriguingly, proteins including synaptic structural proteins and complement C1r, C5, and C9 were found to be upregulated in AD APs but not non-AD APs. Moreover, the proteomic pattern of AD APs was distinct from APP/PS1 APs and exhibited correlation with aging hippocampus. DISCUSSION: Our results provide new insight into AP composition. We demonstrate unexpected differences between AD, non-AD, and APP/PS1 mouse APs, which may relate to different pathological processes.
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A recent proteomics study of amyloid plaques isolated by laser-capture microdissection confirmed five proteins (MDK, NTN1, SMOC1, SLIT2, HTRA1) in plaque areas.
“Additional evidence comes from a recent proteomics study of amyloid plaques isolated by laser-capture microdissection (Xiong et al., 2019), confirming 5 proteins (MDK, NTN1, SMOC1, SLIT2, and HTRA1) in the plaque areas.”
From Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer’s Disease Progression (2020) · cites this paper as Xiong et al., 2019
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Self-citation
- Authors
- Xiong, F., Ge, W., & Ma, C.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Corresponding author
- Ge, Wei
- Ma, Chao
- Venue
- Alzheimer's & Dementia
- Publisher
- Wiley
- Published
- 2018-11-28
- Volume
- 15
- Issue
- 3
- Pages
- 429-440
- ISSN
- 1552-5260 (ISSN-L)
- 1552-5279
- OpenAlex
- W2904603472
- Cited by
- 133
- References
- 52
- Citations by year
- 2026: 14 · 2025: 21 · 2024: 16 · 2023: 19 · 2022: 13 · 2021: 26 · 2020: 17 · 2019: 6 · 2012: 1
- FWCI
- 8.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Alzheimer s & Dementia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81271239, 91632113, 81771205)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- Alzheimer's disease, amyloid plaques
- MeSH terms
- Adult, Aged, 80 and over, Alzheimer Disease, Animals, Brain, Disease Models, Animal, Humans, Mice, Inbred C57BL, Mice, Transgenic, Middle Aged, Amyloid beta-Protein Precursor, Computational Biology, Proteomics, Presenilin-1, Plaque, Amyloid
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