Adaptive laboratory evolution of the fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for improved solvent tolerance
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Cyanobacteria hold promise as cell factories for the photoautotrophic conversion of carbon dioxide to useful chemicals. For the eventual commercial viability of such processes, cyanobacteria need to be engineered for (i) efficient channeling of carbon flux toward the product of interest and (ii) improved product tolerance, the latter being the focus of this study. We chose the recently reported, fast-growing, high light and CO 2 tolerant cyanobacterium Synechococcus elongatus PCC 11801 for adaptive laboratory evolution. In two parallel experiments that lasted over 8400 h of culturing and 100 serial passages, S. elongatus PCC 11801 was evolved to tolerate 5 g/L n-butanol or 30 g/L 2,3-butanediol representing a 100% improvement in concentrations tolerated. The evolved strains retained alcohol tolerance even after being passaged several times without the alcohol stress suggesting that the changes were permanent. Whole genome sequencing of the n-butanol evolved strains revealed mutations in a number of stress responsive genes encoding translation initiation factors, RpoB and an ABC transporter. In 2,3-butanediol evolved strains, genes for ClpC, a different ABC transporter, glyceraldehyde-3-phosphate dehydrogenase and phosphoribulokinase were found to be mutated. Furthermore, the evolved strains showed significant improvement in tolerance toward several other alcohols. Notably, the n-butanol evolved strain could tolerate up to 32 g/L ethanol, thereby making it a promising host for photosynthetic production of biofuels via metabolic engineering.
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Cyanobacterial ALE has already been used to improve solvent tolerance in Synechococcus elongatus PCC 11801 and cadmium tolerance in Synechocystis sp. PCC 6803, while engineered hypermutation has accelerated adaptation of Synechococcus elongatus PCC 7942 to combined high-light and high-temperature stress.
“Cyanobacterial ALE has already been used to improve solvent tolerance in Synechococcus elongatus PCC 11801 [218] and cadmium tolerance in Synechocystis sp. PCC 6803 [219], while engineered hypermutation has accelerated adaptation of Synechococcus elongatus PCC 7942 to combined high-light and high-temperature stress [220].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [218]
Cites
Chlorophyll fluorescence—a practical guide
Maxwell, K., & Johnson, G. N. (2000) · Journal of Experimental Botany · doi:10.1093/jexbot/51.345.659 · Cited by 9,347 · see citing works
Chlorophyll Fluorescence: A Probe of Photosynthesis In Vivo
Baker, N. R. (2008) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.59.032607.092759 · Cited by 4,672 · see citing works
PHOTOTROPHIC PROKARYOTES: THE CYANOBACTERIA
Stanier, R. Y., & Cohen-Bazire, G. (1977) · Annual Review of Microbiology · doi:10.1146/annurev.mi.31.100177.001301 · Cited by 1,015 · cited 1× · see citing works
“3 INTRODUCTION 53 Cyanobacteria, a group of Gram-negative prokaryotes capable of performing oxygenic 54 photosynthesis, are attracting attention as potential microbial cell factories (1).”
BackgroundIron and Oxidative Stress in Bacteria
Touati, D. (2000) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1999.1518 · Cited by 915 · see citing works
Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
Atsumi, S., Higashide, W., & Liao, J. C. (2009) · Nature Biotechnology · doi:10.1038/nbt.1586 · Cited by 870 · see citing works
Adaptive laboratory evolution—Principles and applications for biotechnology
Dragosits, M., & Mattanovich, D. (2013) · Microbial Cell Factories · doi:10.1186/1475-2859-12-64 · Cited by 827 · see citing works
Singlet oxygen production in photosynthesis
Krieger‐Liszkay, A. (2004) · Journal of Experimental Botany · doi:10.1093/jxb/erh237 · Cited by 794 · see citing works
Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli
Shen, C. R., Lan, E. I., Dekishima, Y., Baez, A., Cho, K. M., & Liao, J. C. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.03034-10 · Cited by 623 · see citing works
Oxidative stress inhibits the repair of photodamage to the photosynthetic machinery
Nishiyama, Y., Yamamoto, H., Allakhverdiev, S. I., Inaba, M., Yokota, A., & Murata, N. (2001) · The EMBO Journal · doi:10.1093/emboj/20.20.5587 · Cited by 532 · see citing works
Cell division inhibitors SulA and MinCD prevent formation of the FtsZ ring
Bi, E., & Lutkenhaus, J. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.4.1118-1125.1993 · Cited by 407 · see citing works
Different Trajectories of Parallel Evolution During Viral Adaptation
Wichman, H. A., Badgett, M. R., Scott, L., Boulianne, C. M., & Bull, J. J. (1999) · Science · doi:10.1126/science.285.5426.422 · Cited by 386 · see citing works
Cyanobacterial conversion of carbon dioxide to 2,3-butanediol
Oliver, J. W. K., Machado, I. M. P., Yoneda, H., & Atsumi, S. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1213024110 · Cited by 382 · see citing works
Allahverdiyeva, Y., Mustila, H., Ermakova, M., Bersanini, L., Richaud, P., Ajlani, G., Battchikova, N., Cournac, L., & Aro, E. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1221194110 · Cited by 342 · see citing works
Metabolic engineering of cyanobacteria for ethanol production
Dexter, J., & Fu, P. (2009) · Energy & Environmental Science · doi:10.1039/b811937f · Cited by 329 · see citing works
Tests of parallel molecular evolution in a long-term experiment with Escherichia coli
Woods, R. J., Schneider, D., Winkworth, C. L., Riley, M. A., & Lenski, R. E. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0602917103 · Cited by 316 · see citing works
FtsZ ring formation in fts mutants
Addinall, S. G., Bi, E., & Lutkenhaus, J. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.13.3877-3884.1996 · Cited by 301 · see citing works
Role of Lipids and Fatty Acids in Stress Tolerance in Cyanobacteria
Singh, S., Sinha, R. P., & Haeder, D. P. (2002) · Cited by 262 · see citing works
Tamoi, M., Miyazaki, T., Fukamizo, T., & Shigeoka, S. (2005) · The Plant Journal · doi:10.1111/j.1365-313x.2005.02391.x · Cited by 236 · see citing works
Figueroa, F. L., Conde-Álvarez, R., & Gómez, I. (2003) · Photosynthesis Research · doi:10.1023/a:1023936313544 · Cited by 208 · see citing works
Conrad, T. M., Joyce, A. R., Applebee, M. K., Barrett, C. L., Xie, B., Gao, Y., & Palsson, B. Ø. (2009) · Genome biology · doi:10.1186/gb-2009-10-10-r118 · Cited by 196 · see citing works
Cyanogate: a modular cloning suite for engineering cyanobacteria based on the plant moclo syntax
Vasudevan, R., Gale, G. A. R., Schiavon, A. A., Puzorjov, A., Malin, J., Gillespie, M. D., Vavitsas, K., Zulkower, V., Wang, B., Howe, C. J., et al. (2019) · Plant Physiology · doi:10.1104/pp.18.01401 · Cited by 189 · see citing works
Self-citationMeasurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria
Zavřel, T., Sinetova, M. A., & Cerven�, J. (2015) · BIO-PROTOCOL · doi:10.21769/bioprotoc.1467 · Cited by 187 · see citing works
Inokuma, K., Liao, J. C., Okamoto, M., & Hanai, T. (2010) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2010.07.010 · Cited by 180 · see citing works
Development of Ethanologenic Bacteria
Jarboe, L. R., Grabar, T. B., Yomano, L. P., Shanmugan, K. T., & Ingram, L. O. (2007) · Springer eBooks · doi:10.1007/10_2007_068 · Cited by 174 · see citing works
Xu, Y., Chu, H., Gao, C., Tao, F., Zhou, Z., Li, K., Li, L., Ma, C., & Xu, P. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.02.004 · Cited by 174 · see citing works
Structural basis for the control of translation initiation during stress
Vila‐Sanjurjo, A., Schuwirth, B.-S., Hau, C. W., & Cate, J. H. D. (2004) · Nature Structural & Molecular Biology · doi:10.1038/nsmb850 · Cited by 173 · see citing works
Hirschberg, J., & Chamovitz, D. (1994) · Springer eBooks · doi:10.1007/978-94-011-0227-8_18 · Cited by 169 · see citing works
Two Menkes-type ATPases Supply Copper for Photosynthesis inSynechocystis PCC 6803
Tottey, S., Rich, P. R., Rondet, S., & Robinson, N. J. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m011243200 · Cited by 152 · see citing works
Lan, E. I., Ro, S. Y., & Liao, J. C. (2013) · Energy & Environmental Science · doi:10.1039/c3ee41405a · Cited by 146 · see citing works
Dragosits, M., Mozhayskiy, V., Quiñones-Soto, S., Park, J., & Tagkopoulos, I. (2013) · Molecular Systems Biology · doi:10.1038/msb.2012.76 · Cited by 145 · see citing works
ALEdb 1.0: a database of mutations from adaptive laboratory evolution experimentation
Phaneuf, P. V., Gosting, D., Palsson, B. Ø., & Feist, A. M. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky983 · Cited by 145 · see citing works
Stress Sensors and Signal Transducers in Cyanobacteria
Los, D. A., Zorina, A. A., Sinetova, M. A., Kryazhov, S., Mironov, K. S., & Зинченко, В. В. (2010) · Sensors · doi:10.3390/s100302386 · Cited by 130 · see citing works
Danesi, E. D. G., Rangel‐Yagui, C. D. O., de Carvalho, J. C. M., & Sato, S. (2003) · Biomass and Bioenergy · doi:10.1016/s0961-9534(03)00127-2 · Cited by 122 · see citing works
Jaiswal, D., Sengupta, A., Sohoni, S. V., Sengupta, S., Phadnavis, A. G., Pakrasi, H. B., & Wangikar, P. P. (2018) · Scientific Reports · doi:10.1038/s41598-018-34872-z · Cited by 120 · see citing works
Self-citationGao, K., Yu, H., & Brown, M. T. (2007) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2007.09.006 · Cited by 115 · see citing works
Reyes, L. H., Almario, M. P., Winkler, J. D., Orozco, M. M., & Kao, K. C. (2012) · Metabolic Engineering · doi:10.1016/j.ymben.2012.05.002 · Cited by 112 · see citing works
Kanesaki, Y., Yamamoto, H., Paithoonrangsarid, K., Shumskaya, M. A., Suzuki, I., Hayashi, H., & Murata, N. (2006) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02959.x · Cited by 111 · see citing works
Isobutyraldehyde production from Escherichia coli by removing aldehyde reductase activity
Rodriguez, G. M., & Atsumi, S. (2012) · Microbial Cell Factories · doi:10.1186/1475-2859-11-90 · Cited by 108 · see citing works
Chitnis, P. R., & Nelson, N. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)52401-7 · Cited by 106 · see citing works
Eriksson, M., & Clarke, A. K. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.16.4839-4846.1996 · Cited by 103 · see citing works
The CP12 protein family: a thioredoxin-mediated metabolic switch?
López‐Calcagno, P. E., Howard, T. P., & Raines, C. A. (2014) · Frontiers in Plant Science · doi:10.3389/fpls.2014.00009 · Cited by 103 · see citing works
Tian, X., Chen, L., Wang, J., Qiao, J., & Zhang, W. (2012) · Journal of Proteomics · doi:10.1016/j.jprot.2012.10.002 · Cited by 103 · see citing works
Giner‐Lamia, J., López‐Maury, L., Reyes, J. C., & Florencio, F. J. (2012) · PLANT PHYSIOLOGY · doi:10.1104/pp.112.200659 · Cited by 103 · see citing works
Schulze, K., Hemmerling, D. A. L., Tillich, U. M., & Frohme, M. (2011) · BMC Biotechnology · doi:10.1186/1472-6750-11-118 · Cited by 101 · see citing works
Nakajima, T., Kajihata, S., Yoshikawa, K., Matsuda, F., Furusawa, C., Hirasawa, T., & Shimizu, H. (2014) · Plant and Cell Physiology · doi:10.1093/pcp/pcu091 · Cited by 100 · see citing works
Suzuki, I., Kanesaki, Y., Hayashi, H., Hall, J. J., Simon, W. J., Slabas, A. R., & Murata, N. (2005) · PLANT PHYSIOLOGY · doi:10.1104/pp.104.059097 · Cited by 99 · see citing works
Using Transcriptomics To Improve Butanol Tolerance of Synechocystis sp. Strain PCC 6803
Anfelt, J., Hallström, B. M., Nielsen, J., Uhlén, M., & Hudson, E. P. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.02694-13 · Cited by 95 · see citing works
Porankiewicz, J., & Clarke, A. K. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.16.5111-5117.1997 · Cited by 81 · see citing works
Recent advances in synthetic biology of cyanobacteria
Sengupta, A., Pakrasi, H. B., & Wangikar, P. P. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-9046-x · Cited by 78 · see citing works
Self-citationRole for the Cyanobacterial HtpG in Protection from Oxidative Stress
Hossain, M. M., & Nakamoto, H. (2002) · Current Microbiology · doi:10.1007/s00284-002-3831-5 · Cited by 77 · see citing works
Liu, X., Gu, Q., & Yu, X. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.12.121 · Cited by 75 · see citing works
Hendry, J. I., Prasannan, C. B., Ma, F., Möllers, K. B., Jaiswal, D., Digmurti, M. G., Allen, D. K., Frigaard, N., Dasgupta, S., & Wangikar, P. P. (2017) · Biotechnology and Bioengineering · doi:10.1002/bit.26350 · Cited by 74 · see citing works
Self-citationPhotoprotective, excited-state quenching mechanisms in diverse photosynthetic organisms
Magdaong, N. C. M., & Blankenship, R. E. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.tm117.000233 · Cited by 72 · see citing works
Future of microbial polyesters
Lee, G. B., & Na, J. (2013) · Microbial Cell Factories · doi:10.1186/1475-2859-12-54 · Cited by 69 · see citing works
Wang, Y., Shi, M., Niu, X., Zhang, X., Gao, L., Chen, L., Wang, J., & Zhang, W. (2014) · Microbial Cell Factories · doi:10.1186/s12934-014-0151-y · Cited by 66 · see citing works
Kaczmarzyk, D., Anfelt, J., Särnegrim, A., & Hudson, E. P. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.04.017 · Cited by 63 · see citing works
Sengupta, S., Jaiswal, D., Sengupta, A., Shah, S., Gadagkar, S., & Wangikar, P. P. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-01727-7 · Cited by 62 · see citing works
Self-citationCold-stress-induced de novo expression of infC and role of IF3 in cold-shock translational bias
Giuliodori, A. M., Brandi, A., Giangrossi, M., Gualerzi, C. O., & Pon, C. L. (2007) · RNA · doi:10.1261/rna.455607 · Cited by 61 · see citing works
Adaptive laboratory evolution of cadmium tolerance in Synechocystis sp. PCC 6803
Xu, C., Sun, T., Li, S., Chen, L., & Zhang, W. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1205-x · Cited by 59 · see citing works
Sengupta, A., Pritam, P., Jaiswal, D., Bandyopadhyay, A., Pakrasi, H. B., & Wangikar, P. P. (2020) · Metabolites · doi:10.3390/metabo10060250 · Cited by 52 · see citing works
Self-citationMatsusako, T., Toya, Y., Yoshikawa, K., & Shimizu, H. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0996-5 · Cited by 44 · see citing works
Light-dependent governance of cell shape dimensions in cyanobacteria
Montgomery, B. L. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00514 · Cited by 36 · see citing works
Towijit, U., Songruk, N., Lindblad, P. C., Incharoensakdi, A., & Jantaro, S. (2018) · Scientific Reports · doi:10.1038/s41598-018-31789-5 · Cited by 36 · see citing works
Jaiswal, D., Prasannan, C. B., Hendry, J. I., & Wangikar, P. P. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.7b05329 · Cited by 32 · see citing works
Self-citationBañares‐España, E., Kromkamp, J. C., López‐Rodas, V., Costas, E., & Flores‐Moya, A. (2012) · FEMS Microbiology Ecology · doi:10.1111/1574-6941.12025 · Cited by 29 · see citing works
Varshney, P., Beardall, J., Bhattacharya, S., & Wangikar, P. P. (2019) · Journal of Applied Phycology · doi:10.1007/s10811-019-01950-2 · Cited by 29 · see citing works
Self-citationTahara, H., Uchiyama, J., Yoshihara, T., Matsumoto, K., & Ohta, H. (2012) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2012.02.035 · Cited by 25 · see citing works
Sun, T., Pei, G., Wang, J., Chen, L., & Zhang, W. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0727-y · Cited by 24 · see citing works
The Min Oscillator Defines Sites of Asymmetric Cell Division in Cyanobacteria during Stress Recovery
Liao, Y., & Rust, M. J. (2018) · Cell Systems · doi:10.1016/j.cels.2018.10.006 · Cited by 17 · see citing works
Singh, K. P., Wangikar, P. P., & Jadhav, S. (2011) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-011-0995-7 · Cited by 16 · see citing works
Self-citationPrabha, G. L., & Kulandaivelu, G. (2002) · Plant Science · doi:10.1016/s0168-9452(02)00008-0 · Cited by 13 · see citing works
Xu, X. Z., Zhang, J. Y., Zhang, G. H., Long, G. Q., Yang, S. C., Chen, Z. J., Wei, F. G., & Chen, J. W. (2018) · PubMed · doi:10.13287/j.1001-9332.201801.008 · Cited by 12 · see citing works
Nesbit, A. D., Whippo, C. W., Hangarter, R. P., & Kehoe, D. M. (2015) · Photosynthesis Research · doi:10.1007/s11120-015-0074-4 · Cited by 11 · see citing works
Arai, S., Hayashihara, K., Kanamoto, Y., Shimizu, K., Hirokawa, Y., Hanai, T., Murakami, A., & Honda, H. (2017) · Biotechnology and Bioengineering · doi:10.1002/bit.26307 · Cited by 11 · see citing works
Srivastava, R. K., Jaiswal, R., Panda, D., & Wangikar, P. P. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.22184 · Cited by 8 · see citing works
Self-citationHirokawa, Y., Kanesaki, Y., Arai, S., Saruta, F., Hayashihara, K., Murakami, A., Shimizu, K., Honda, H., Yoshikawa, H., & Hanai, T. (2017) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2017.11.012 · Cited by 8 · see citing works
Comparison of ethanol tolerance between potential cyanobacterial production hosts
Kämäräinen, J., Nylund, M., Aro, E., & Kallio, P. (2018) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2018.07.034 · Cited by 8 · see citing works
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Joshi, A., VERMA, K. K., Rajput, V. D., Minkina, T., & Arora, J. (2022) · Bioengineered · doi:10.1080/21655979.2022.2051856 · Cited by 117 · see citing works
Recent progress in adaptive laboratory evolution of industrial microorganisms
Wang, G., Li, Q., Zhang, Z., Yin, X., Wang, B., & Yang, X. (2022) · Journal of Industrial Microbiology & Biotechnology · doi:10.1093/jimb/kuac023 · Cited by 116 · see citing works
Jaiswal, D., Sahasrabuddhe, D., & Wangikar, P. P. (2021) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2021.09.010 · Cited by 44 · see citing works
Self-citationLight and carbon: Synthetic biology toward new cyanobacteria-based living biomaterials
Goodchild-Michelman, I. M., Church, G. M., Schubert, M. G., & Tang, T.-C. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100583 · Cited by 41 · see citing works
Fast-growing cyanobacterial chassis for synthetic biology application
Li, Z., Li, S., Chen, L., Sun, T., & Zhang, W. (2023) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2023.2166455 · Cited by 30 · see citing works
Application of Microalgal Stress Responses in Industrial Microalgal Production Systems
Wang, J., Wang, Y., Wu, Y., Fan, Y., Zhu, C., Fu, X., Chu, Y., Chen, F., Sun, H., & Mou, H. (2021) · Marine Drugs · doi:10.3390/md20010030 · Cited by 26 · see citing works
Andrews, F., Faulkner, M., Toogood, H. S., & Scrutton, N. S. (2021) · Biotechnology for Biofuels · doi:10.1186/s13068-021-02091-w · Cited by 23 · see citing works
Application of Cyanobacteria as Chassis Cells in Synthetic Biology
Liu, X., Tang, K., & Hu, J. (2024) · Microorganisms · doi:10.3390/microorganisms12071375 · Cited by 20 · see citing works
Theodosiou, E., Tüllinghoff, A., Toepel, J., & Bühler, B. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.855715 · Cited by 17 · see citing works
Sengupta, S., Sahasrabuddhe, D., & Wangikar, P. P. (2022) · Biotechnology Advances · doi:10.1016/j.biotechadv.2021.107816 · Cited by 16 · see citing works
Self-citationExploring cyanobacterial diversity for sustainable biotechnology
Selão, T. T. (2022) · Journal of Experimental Botany · doi:10.1093/jxb/erac053 · Cited by 15 · see citing works
Current insights into molecular mechanisms of environmental stress tolerance in Cyanobacteria
Rai, P., Pathania, R., Bhagat, N., Bongirwar, R., Shukla, P., & Srivastava, S. (2025) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-025-04260-7 · Cited by 18 · see citing works
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Sengupta, A., Liu, D., & Pakrasi, H. B. (2022) · Methods in Enzymology · doi:10.1016/bs.mie.2022.07.023 · Cited by 13 · see citing works
Jain, V. S., Schubert, M. G., Sarnaik, A. P., Pritam, P., Jaiswal, D., Church, G. M., & Wangikar, P. P. (2024) · The Microbe · doi:10.1016/j.microb.2024.100048 · Cited by 8 · see citing works
Self-citationMetabolic engineering of fast growing cyanobacteria for phototrophic production of 2,3-butanediol
Srivastava, V., & Wangikar, P. P. (2024) · Biochemical Engineering Journal · doi:10.1016/j.bej.2024.109439 · Cited by 6 · see citing works
Self-citationSrivastava, V., Sarnaik, A. P., & Wangikar, P. P. (2024) · Biotechnology Progress · doi:10.1002/btpr.3509 · Cited by 6 · see citing works
Self-citationMutations in the circadian cycle drive adaptive plasticity in cyanobacteria
Mendaña, A., Santos‐Merino, M., Gutiérrez-Lanza, R., Domínguez-Quintero, M., Medina, J. M., González-Guerra, A., Campa, V. M., Baez, M., Ducos‐Galand, M., López‐Igual, R., et al. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2506928122 · Cited by 5 · see citing works
Liu, Y., Danial, M., Dong, H., Zhang, M., Jia, R., & Zhang, G. (2023) · Food Bioengineering · doi:10.1002/fbe2.12039 · Cited by 3 · see citing works
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Stressing cyanobacteria for enhanced production of bulk and value-added chemicals
Mo, G., Xu, X., Sun, H., Sun, J., Mao, S., Cui, J., Luan, G., & Lü, X. (2025) · Algal Research · doi:10.1016/j.algal.2025.104095 · Cited by 2 · see citing works
Carbon capture, storage, and utilization in cyanobacteria
Majhi, B. K. (2026) · Carbon Resources Conversion · doi:10.1016/j.crcon.2025.100388 · Cited by 2 · see citing works
Wada, K., Uebayashi, K., Toya, Y., Putri, S. P., Matsuda, F., Fukusaki, E., Liao, J. C., & Shimizu, H. (2023) · The Journal of General and Applied Microbiology · doi:10.2323/jgam.2023.03.002 · Cited by 2 · see citing works
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Self-citationWalshe, J., & Saha, S. K. (2026) · Current Issues in Molecular Biology · doi:10.3390/cimb48060638 · Cited by 1 · see citing works
“Cyanobacterial ALE has already been used to improve solvent tolerance in Synechococcus elongatus PCC 11801 [218] and cadmium tolerance in Synechocystis sp. PCC 6803 [219], while engineered hypermutation has accelerated adaptation of Synechococcus elongatus PCC 7942 to combined high-light and…”
Zhang, W., Zhou, J., Zhang, Y., & Li, Y. (2025) · Systems Microbiology and Biomanufacturing · doi:10.1007/s43393-025-00391-y · Cited by 1 · see citing works
Karayannis, D., Limnaiou, N. V., Rondags, E., Chevalot, I., & Papanikolaou, S. (2025) · Bioresource Technology Reports · doi:10.1016/j.biteb.2025.102477 · Cited by 1 · see citing works
Wang, W., Pan, K., Gao, J., Chen, L., Sun, T., & Zhang, W. (2026) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2026.111384 · Cited by 1 · see citing works
Shaberi, H. S. A., Bunawan, H., Craig, S., Bryan, S. J., & Ramzi, A. B. (2024) · Algal Research · doi:10.1016/j.algal.2024.103755 · Cited by 1 · see citing works
Leveraging Cyanobacteria for Alleviating Climate Change-Induced Abiotic Stress in Crop Plants
Rani, V., Jaiswal, P., Mahawar, H., Khati, P., Arora, A., & Musa, I. O. (2026) · Springer eBooks · doi:10.1007/978-981-95-8883-1_15
- Authors
- Srivastava, V., Amanna, R., Rowden, S. J. L., Sengupta, S., Madhu, S., Howe, C. J., & Wangikar, P. P.
- Affiliations
- Indian Institute of Technology Bombay (IN)
- University of Cambridge (GB)
- Corresponding author
- Wangikar, Pramod P.
- Venue
- Journal of Bioscience and Bioengineering
- Publisher
- Elsevier BV
- Published
- 2021-05
- Volume
- 131
- Issue
- 5
- Pages
- 491-500
- ISSN
- 1347-4421 (ISSN-L)
- 1389-1723
- OpenAlex
- W3120133637
- Cited by
- 29
- References
- 77
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 4
- FWCI
- 1.49
- Type
- journal article
- Language
- en
- Open access
- Licence
- Locations
- Journal of Bioscience and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Apollo (University of Cambridge) (submittedVersion, cc-by-nc-nd)
- Apollo
- Funders
- Department of Biotechnology , Ministry of Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- adaptive laboratory evolution, solvent tolerance, ethanol tolerance, stress response genes, translation initiation factors, rpoB, ABC transporters, ClpC, glyceraldehyde-3-phosphate dehydrogenase, phosphoribulokinase, biofuel production
- MeSH terms
- Alcohols, Carbon Dioxide, Photosynthesis, Solvents, Directed Molecular Evolution, Synechococcus, Biofuels
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