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Cited by 61Algal biology and biofuel productionSeaweed-derived Bioactive CompoundsAquaculture Nutrition and Growth

Effects of Nitrogen Availability on the Antioxidant Activity and Carotenoid Content of the Microalgae Nephroselmis sp

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Coulombier, Noémie, Nicolau, Elodie, Le Déan, Loïc, Barthelemy, Vanille, Schreiber, Nathalie, Brun, Pierre, Lebouvier, Nicolas, Jauffrais, Thierry·2020·Marine Drugs
DOI 10.3390/md18090453PMC PMC7551860
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Abstract

Nephroselmis sp. was previously identified as a species of interest for its antioxidant properties owing to its high carotenoid content. In addition, nitrogen availability can impact biomass and specific metabolites’ production of microalgae. To optimize parameters of antioxidant production, Nephroselmis sp. was cultivated in batch and continuous culture conditions in stirred closed photobioreactors under different nitrogen conditions (N-repletion, N-limitation, and N-starvation). The aim was to determine the influence of nitrogen availability on the peroxyl radical scavenging activity (oxygen radical absorbance capacity (ORAC) assay) and carotenoid content of Nephroselmis sp. Pigment analysis revealed a specific and unusual photosynthetic system with siphonaxanthin-type light harvesting complexes found in primitive green algae, but also high lutein content and xanthophyll cycle pigments (i.e., violaxanthin, antheraxanthin, and zeaxanthin), as observed in most advanced chlorophytes. The results indicated that N-replete conditions enhance carotenoid biosynthesis, which would correspond to a higher antioxidant capacity measured in Nephroselmis sp. Indeed, peroxyl radical scavenging activity and total carotenoids were higher under N-replete conditions and decreased sharply under N-limitation or starvation conditions. Considering individual carotenoids, siphonaxanthin, neoxanthin, xanthophyll cycle pigments, and lycopene followed the same trend as total carotenoids, while β-carotene and lutein stayed stable regardless of the nitrogen availability. Carotenoid productivities were also higher under N-replete treatment. The peroxyl radical scavenging activity measured with ORAC assay (63.6 to 154.9 µmol TE g−1 DW) and the lutein content (5.22 to 7.97 mg g−1 DW) were within the upper ranges of values reported previously for other microalgae. Furthermore, contents of siphonaxanthin ere 6 to 20% higher than in previous identified sources (siphonous green algae). These results highlight the potential of Nephroselmis sp. as a source of natural antioxidant and as a pigment of interest.

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  • Coulombier et al. (2020) conducted experiments under nitrogen starvation conditions in 10L photobioreactors for 28 days and observed increased lutein content (5.22-7.97 mg g−1 DW) under nitrogen replete conditions.

    “Coulombier et al. (2020) conducted the experiments under nitrogen starvation conditions in 10L photobioreactors for 28 days and observed increased lutein content (5.22–7.97 mg g−1 DW) under nitrogen replete conditions.”

    From Production of microalgae with high lipid content and their potential as sources of nutraceuticals (2023) · cites this paper as Coulombier et al. 2020

    Materials and Methods (§4.3; page unavailable) confirms the use of two 10 L photobioreactors and nitrogen-starvation periods, but the experiment ran through day 31, with a second starvation period beginning on day 28—not 28 days entirely under starvation. Results (§2.3) gives the stated lutein range of 5.22–7.97 mg g−1 DW, but says concentrations were stable throughout the experiment; the Discussion (§3) likewise says lutein remained stable regardless of nitrogen availability, rather than increasing under nitrogen-replete conditions. (LLM, conf=0.90)

    Experimental — accuracy not yet measured

  • Lycopene production has been evaluated by several microalgal cultures such as Chlorella vulgaris, Nephroselmis sp, Dunaliella salina, etc.

    “Lycopene production has been evaluated by several microalgal cultures such as Chlorella vulgaris, Nephroselmis sp, Dunaliella salina, etc. (Coulombier et al. 2020; Mazzucchi et al. 2020; Mtaki et al. 2020).”

    From Production of microalgae with high lipid content and their potential as sources of nutraceuticals (2023) · cites this paper as Coulombier et al. 2020

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Cited by

Sorted by citations

Authors
Coulombier, N., Nicolau, E., Le Déan, L., Barthelemy, V., Schreiber, N., Brun, P., Lebouvier, N., & Jauffrais, T.
Affiliations
  • ADECAL Technopole, 1 bis rue Berthelot, 98846 Noumea, New Caledonia
  • Ifremer, RBE/BRM/LPBA, Rue de l’île d’Yeu, 44311 Nantes, France
  • Ifremer, IRD, Univ Nouvelle-Calédonie, Univ La Réunion, UMR 9220 ENTROPIE, BP 32078, 98800 Nouméa, New Caledonia
  • University of New Caledonia (NC)
  • Ifremer (FR)
Corresponding author
Coulombier, Noémie
ORCID
Venue
Marine Drugs
Publisher
MDPI AG
Published
2020-08-29
Volume
18
Issue
9
Pages
453
ISSN
1660-3397 (ISSN-L)
DOI
10.3390/md18090453
PMC
PMC7551860
OpenAlex
W3082553406
Cited by
61
References
10
Citations by year
2026: 5 · 2025: 14 · 2024: 10 · 2023: 11 · 2022: 11 · 2021: 8 · 2020: 1
FWCI
1.53
Type
journal article
Language
en
Open access
Licence
Locations
  • Marine Drugs (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • Institutional Archive of Ifremer (French Research Institute for Exploitation of the Sea) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • New Caledonia Government (no)
  • Province Sud of New Caledonia (no)
  • Province Nord of New Caledonia (no)
  • Comité Interministeriel de l'Outre Mer (no)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Keywords
nitrogen availability, nitrogen limitation, nitrogen starvation, antioxidant activity, carotenoids, lutein, siphonaxanthin, xanthophyll cycle pigments, peroxyl radical scavenging, green algae, microalgae, carotenoid biosynthesis, natural antioxidants, photobioreactors
MeSH terms
Oxygen Radical Absorbance Capacity, Chlorophyta, Antioxidants, Carotenoids, Nitrogen, Time Factors, Biomass, Microalgae

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