The DOI matches its Crossref record; 116 of the 116 references it cites were found in a registry or an index.
Ballistic labeling and dynamic imaging of astrocytes in organotypic hippocampal slice cultures.
Abstract
Protoplasmic astrocytes in mammalian CNS tissues in vivo have a highly complex 3D morphology, but in dissociated cell cultures they often assume a flattened, fibroblast-like morphology bearing only a few, simple processes. By fluorescent labeling and confocal reconstruction we show that many astrocytes in organotypic hippocampal slice cultures exhibit a more native complex cytoarchitecture. Although astrocytes at the surface of slice cultures show a reactive form with several thick glial fibrillary acidic protein (GFAP)-positive processes, astrocytes situated in deeper portions of tissue slices retain a highly complex 3D morphology with many fine spine- or veil-like protrusions. Dozens of astrocytes can be labeled in single slice cultures by gene gun-mediated ballistic delivery of gold or tungsten particles carrying cDNAs (Biolistics), lipophilic dyes (DiOlistics), or fluorescent intracellular calcium indicators (Calistics). Expression of a membrane-targeted form of eGFP (Lck-GFP) is superior to soluble eGFP for resolving fine astrocytic processes. Time-lapse confocal imaging of Lck-GFP transfected astrocytes or "calistically" labeled astrocytes show structural remodeling and calcium transients, respectively. This approach provides an in vitro system for investigating the functional architecture, development and dynamic remodeling of astrocytes and their relationships to neurons and glia in live mammalian brain tissues.
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“Histological visualization with the fluorescent reporter mCherry confirmed that Gq-DREADD expression remained restricted to astrocytes based on their co-localization with the astrocyte marker GFAP (Figure 1C) and morphology consistent with astrocytic immunoreactivity patterns (Benediktsson et al., 2005;Bushong et al., 2002;Scofield et al., 2015).”
From Single-cell resolution analysis of the crosstalk between chemogenically activated astrocytes and microglia (2020) · cites this paper as Benediktsson et al., 2005
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Uniquely Hominid Features of Adult Human Astrocytes
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Gq-DREADD Selectively Initiates Glial Glutamate Release and Inhibits Cue-induced Cocaine Seeking
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“…mCherry confirmed that Gq-DREADD expression remained restricted to astrocytes based on their co-localization with the astrocyte marker GFAP (Figure 1C) and morphology consistent with astrocytic immunoreactivity patterns (Benediktsson et al., 2005; Bushong et al., 2002; Scofield et al., 2015).”
Background
Metadata
- Authors
- Benediktsson, A. M., Schachtele, S. J., Green, S. H., & Dailey, M. E.
- Venue
- Journal of Neuroscience Methods
- Publisher
- Elsevier BV
- Published
- 2005-01
- Volume
- 141
- Issue
- 1
- Pages
- 41-53
- ISSN
- 0165-0270
- Cited by
- 166
- References
- 116
- Language
- en
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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