Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 166

The DOI matches its Crossref record; 116 of the 116 references it cites were found in a registry or an index.

Ballistic labeling and dynamic imaging of astrocytes in organotypic hippocampal slice cultures.

Benediktsson, Adrienne M., Schachtele, Scott J., Green, Steven H., Dailey, Michael E.·2005·Journal of Neuroscience Methods
DOI 10.1016/j.jneumeth.2004.05.013
Open this paper in CoChat — free account

Abstract

Protoplasmic astrocytes in mammalian CNS tissues in vivo have a highly complex 3D morphology, but in dissociated cell cultures they often assume a flattened, fibroblast-like morphology bearing only a few, simple processes. By fluorescent labeling and confocal reconstruction we show that many astrocytes in organotypic hippocampal slice cultures exhibit a more native complex cytoarchitecture. Although astrocytes at the surface of slice cultures show a reactive form with several thick glial fibrillary acidic protein (GFAP)-positive processes, astrocytes situated in deeper portions of tissue slices retain a highly complex 3D morphology with many fine spine- or veil-like protrusions. Dozens of astrocytes can be labeled in single slice cultures by gene gun-mediated ballistic delivery of gold or tungsten particles carrying cDNAs (Biolistics), lipophilic dyes (DiOlistics), or fluorescent intracellular calcium indicators (Calistics). Expression of a membrane-targeted form of eGFP (Lck-GFP) is superior to soluble eGFP for resolving fine astrocytic processes. Time-lapse confocal imaging of Lck-GFP transfected astrocytes or "calistically" labeled astrocytes show structural remodeling and calcium transients, respectively. This approach provides an in vitro system for investigating the functional architecture, development and dynamic remodeling of astrocytes and their relationships to neurons and glia in live mammalian brain tissues.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.jneumeth.2004.05.013

Cites

116 of 116 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 6

  1. Chao TI, Rickmann M, Wolff JR. The synapse-astrocyte boundary: an anatomical basis for an integrative role of glia in synaptic transmission. In: Volterra A, Magistretti PJ, Haydon PG, editors. The Tripartite Synapse: Glia in synaptic transmission. Great Britain: Oxford University Press; 2002. p. 3–23.
  2. Deitmer JW. pH Regulation. In: Kettenmann H, Ransom BR, editors. Neuroglia. Great Britain: Oxford University Press; 1995. p. 230–245.
  3. Kettenmann H, Ransom BR, Neuroglia. New York: Oxford University Press Oxford; 1995.
  4. Newman EA. Glial cell regulation of extracellular potassium. In: Kettenmann H, Ransom BR, editors. Neuroglia. New York: Oxford University Press Oxford; 1995. p. 717–731.
  5. Privat A, Gimenez-Ribott M, Ridet J-L. Morphology of astrocytes. In: Kettenmann H, Ransom BR, editors. Neuroglia. New York: Oxford University Press Oxford; 1995. p. 3–22.
  6. Volterra A, Magistretti PJ, Haydon PG. The tripartite synapse: glia in synaptic transmission. Great Britain: Oxford University Press; 2002.

Cited by

See all 166

Metadata

Authors
Benediktsson, A. M., Schachtele, S. J., Green, S. H., & Dailey, M. E.
Venue
Journal of Neuroscience Methods
Publisher
Elsevier BV
Published
2005-01
Volume
141
Issue
1
Pages
41-53
ISSN
0165-0270
DOI
10.1016/j.jneumeth.2004.05.013
Cited by
166
References
116
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.