Cited by 52Air Quality and Health ImpactsGenomics, phytochemicals, and oxidative stressNeutrophil, Myeloperoxidase and Oxidative Mechanisms

Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure

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Romani, Arianna, Cervellati, Carlo, Muresan, Ximena M, Belmonte, Giuseppe, Pecorelli, Alessandra, Cervellati, Franco, Benedusi, Mascia, Evelson, Pablo +1 more·2018·Mechanisms of Ageing and Development

Abstract

Epidemiological evidences have correlated airbone particulate matter (PM) to adverse health effects, mainly linking to pulmonary and cardiovascular disease. Nevertheless, only recently, some studies reported detrimental effects of PM on other organs such as skin. In a recent work, we have reported increased oxidative and inflammatory responses in Reconstituted Human Epidermis (RHE) exposed to ambient particles (CAPs) and we also demonstrated the ability of CAPs to penetrate the skin tissue. The present study was aimed to better understand the cellular mechanisms beyond the oxidative changes induced by CAPs (5-10-25μg/mL) in human immortalized keratinocytes (HaCaT). After 24h of treatment, CAPs were able to enter the cells leading to a decrease in viability, increased levels of 4-hydroxinonenal products (4-HNE) and IL-1α release. Overall these data, suggest lipid and protein oxidative damage, as well as an increase of inflammatory response after being challenged with CAPs. In addition, 3h after CAPs exposure we found a significant increase in NF-kB and Nrf2 translocation into the nucleus. In contrast, no differences in gene expression and enzymatic activity of Nrf2 target genes were detected. This last finding could be explained by the ability of CAPs to possibly alter the binding of Nrf2 to the ARE DNA sequence.

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Crossref · 10.1016/j.mad.2017.11.007

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  • Oxidative stress responses were observed in HaCaT cells exposed to concentrated air particles and in primary human keratinocytes exposed to cigarette smoke condensate or diesel particulate extract.

    “Oxidative stress responses were also observed in HaCaT cells exposed to concentrated air particles47 and in primary human keratinocytes exposed to cigarette smoke condensate48 or diesel particulate extract.49”

    From The impact of airborne pollution on skin (2019) · cites this paper as [47]

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Authors
Romani, A., Cervellati, C., Muresan, X. M., Belmonte, G., Pecorelli, A., Cervellati, F., Benedusi, M., Evelson, P., & Valacchi, G.
Affiliations
  • University of Ferrara (IT)
  • North Carolina State University (US)
  • Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
Corresponding author
Valacchi, G.
Venue
Mechanisms of Ageing and Development
Publisher
Elsevier BV
Published
2018-06
Volume
172
Pages
86-95
ISSN
0047-6374 (ISSN-L)
OpenAlex
W2768046897
Cited by
52
References
53
Citations by year
2026: 3 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 7 · 2021: 4 · 2020: 12 · 2019: 11 · 2018: 2
FWCI
3.69
Type
journal article
Language
en
Open access
Locations
  • Mechanisms of Ageing and Development (publishedVersion)
  • PubMed (publishedVersion)
  • CONICET Digital (CONICET) (publishedVersion, cc-by-nc-sa)
  • Conicet (submittedVersion, cc-by-nc-sa)
  • Institutional Research Information System University of Ferrara (University of Ferrara) (submittedVersion, cc-by-nc-nd)
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
particulate matter, air pollution, keratinocytes, oxidative stress, lipid peroxidation, 4-hydroxynonenal, IL-1α, NF-kB, Nrf2, antioxidant response element, skin inflammation, HaCaT keratinocytes
MeSH terms
Cell Line, Transformed, Humans, Oxidation-Reduction, Keratinocytes, NF-kappa B, NF-E2-Related Factor 2, Particulate Matter, Interleukin-1alpha

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