Diversity, classification and evolution of CRISPR-Cas systems
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Items 201–300 of 999
- Advances in delivery systems for CRISPR/Cas-mediated cancer treatment: a focus on viral vectors and extracellular vesiclesZhidu Song, Ying Tao, Yue Liu, Jian Li · 2024 · Frontiers in Immunology
- Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2Annoj Thavalingam, Zhi Cheng, Bianca Garcı́a, Xue Huang, Megha K Shah, Wei Dong Sun, Min Wang, Lucas B. Harrington, Sungwon Hwang, Yurima Hidalgo-Reyes et al. · 2019 · Nature Communications
- Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6Olivier Fuchsbauer, Paolo Swuec, Claire Zimberger, Béatrice Amigues, Sébastien Levesque, Daniel Agudelo, Alexis Duringer, Antonio Chaves-Sanjuán, Silvia Spinelli, Geneviève M. Rousseau et al. · 2019 · Molecular Cell
- Phylogenomics of Cas4 family nucleasesS. Hudaiberdiev, S. Shmakov, Y. Wolf, M. Terns, K. Makarova, E. Koonin · 2017 · BMC Evolutionary Biology
- Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9Daniel Agudelo, Sophie Carter, Minja Velimirovic, Alexis Duringer, Jean-François Rivest, Sébastien Levesque, Jérémy Loehr, Mathilde Mouchiroud, Denis Cyr, Paula J. Waters et al. · 2020 · Genome Research
- Development and Application of CRISPR/Cas in Microbial BiotechnologyWentao Ding, Yang Zhang, Shuobo Shi · 2020 · Frontiers in Bioengineering and Biotechnology
- Potential Application of the CRISPR/Cas9 System against Herpesvirus InfectionsYuan‐Chuan Chen, Jingxue Sheng, Phong Trang, Fenyong Liu · 2018 · Viruses
- CRISPR-Cas System of a Prevalent Human Gut Bacterium Reveals Hyper-targeting against Phages in a Human Virome CatalogPaola Soto-Perez, Jordan E. Bisanz, Joel D. Berry, Kathy N. Lam, Joseph Bondy‐Denomy, Peter James Turnbaugh · 2019 · Cell Host & Microbe
- CRISPR-Cas System in Antibiotic Resistance Plasmids in Klebsiella pneumoniaeMuhammad Kamruzzaman, Jonathan R. Iredell · 2020 · Frontiers in Microbiology
- Cas11 enables genome engineering in human cells with compact CRISPR-Cas3 systemsRenke Tan, Ryan Krueger, Max J. Gramelspacher, Xufei Zhou, Yibei Xiao, Ailong Ke, Zhonggang Hou, Yan Zhang · 2022 · Molecular Cell
- Bacteriophages as Biotechnological ToolsMariana Alves Elois, Raphael da Silva, Giulia Von Tönnemann Pilati, David Rodrı́guez-Làzaro, Gislaine Fongaro · 2023 · Viruses
- The host-encoded RNase E endonuclease as the crRNA maturation enzyme in a CRISPR–Cas subtype III-Bv systemJuliane Behler, Kundan Sharma, Viktoria Reimann, Annegret Wilde, Henning Urlaub, Wolfgang R. Hess · 2018 · Nature Microbiology
- RNA-Based Technologies for Engineering Plant Virus ResistanceMichael E. Taliansky, В.О. Самарская, С. К. Завриев, Igor A. Fesenko, Natalia O. Kalinina, Andrew J. Love · 2021 · Plants
- Classification of CRISPR/Cas system and its application in tomato breedingAbira Chaudhuri, Koushik Halder, Asis Datta · 2022 · Theoretical and Applied Genetics
- Cas13d: A New Molecular Scissor for Transcriptome EngineeringRahul Kumar Gupta, Arijit Ghosh, Rudra Chakravarti, Rajveer Singh, V. Ravichandiran, Snehasikta Swarnakar, Dipanjan Ghosh · 2022 · Frontiers in Cell and Developmental Biology
- CRISPR-Based Epigenome Editing: Mechanisms and ApplicationsShaima M Fadul, Aleeza Arshad, Rashid Mehmood · 2023 · Epigenomics
- An Attenuated CRISPR-Cas System in Enterococcus faecalis Permits DNA AcquisitionKarthik Hullahalli, Marinelle Rodrigues, Uyen Thy Nguyen, Kelli L. Palmer · 2018 · mBio
- Argonaute Proteins and Mechanisms of RNA Interference in Eukaryotes and ProkaryotesA. V. Olina, Andrey V. Kulbachinskiy, Alexei A. Aravin, Daria Esyunina · 2018 · Biochemistry (Moscow)
- Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variantsEszter Tóth, Bernadett C Czene, Péter István Kulcsár, Sarah L Krausz, András Tálas, Antal Nyeste, Éva Varga, Krisztina Huszár, Nóra Weinhardt, Zoltán Ligeti et al. · 2018 · Nucleic Acids Research
- Making the cut(s): how Cas12a cleaves target and non-target DNADaan C. Swarts · 2019 · Biochemical Society Transactions
- Gold Nanomaterials‐Implemented CRISPR‐Cas Systems for BiosensingRuijie Fu, Yunlei Xianyu · 2023 · Small
- Mechanisms for target recognition and cleavage by the Cas12i RNA-guided endonucleaseHeng Zhang, Zhuang Li, Renjian Xiao, Leifu Chang · 2020 · Nature Structural & Molecular Biology
- CRISPR–Cas12a-mediated DNA clamping triggers target-strand cleavageMohsin M. Naqvi, Laura J. Lee, Oscar Enrique Torres Montaguth, Fiona M. Diffin, Mark D. Szczelkun · 2022 · Nature Chemical Biology
- Innovative approaches to combat antibiotic resistance: integrating CRISPR/Cas9 and nanoparticles against biofilm-driven infectionsAbolfazl Saffari Natanzi, Mohsen Poudineh, Elham Karimi, Azad Khaledi, Hamed Haddad Kashani · 2025 · BMC Medicine
- Strategies for Editing Virulent Staphylococcal Phages Using CRISPR-Cas10S. M. Nayeemul Bari, Forrest C. Walker, Katie Cater, Barbaros Aslan, Asma Hatoum-Aslan · 2017 · ACS Synthetic Biology
- Characterization and Exploitation of CRISPR Loci in Bifidobacterium longumClaudio Hidalgo-Cantabrana, Alexandra B. Crawley, Borja Sánchez, Rodolphe Barrangou · 2017 · Frontiers in Microbiology
- Genome editing technology and application in soybean improvementAili Bao, Chanjuan Zhang, Yi Huang, Haifeng Chen, Xinan Zhou, Dong Cao · 2020 · Oil Crop Science
- Cas14a1-mediated nucleic acid detectifon platform for pathogensXiaolin Ge, Tian Meng, Xiao Tan, Yangdao Wei, Zhenzhen Tao, Zhiqing Yang, Fengge Song, Peng Wang, Yi Wan · 2021 · Biosensors and Bioelectronics
- Next-Generation Diagnostic with CRISPR/Cas: Beyond Nucleic Acid DetectionPooja Bhardwaj, Rajni Kant, Sthita Pragnya Behera, Gaurav Raj Dwivedi, Rajeev Singh · 2022 · International Journal of Molecular Sciences
- Characterization of CRISPR-Cas Systems in Clinical Klebsiella pneumoniae Isolates Uncovers Its Potential Association With Antibiotic SusceptibilityHsin-Yu Li, Cheng‐Yen Kao, Wei‐Hung Lin, Po‐Xing Zheng, Jing-Jou Yan, Ming-Cheng Wang, Ching‐Hao Teng, Chin‐Chung Tseng, Jiunn-Jong Wu · 2018 · Frontiers in Microbiology
- Harnessing CRISPR-Cas systems for precision engineering of designer probiotic lactobacilliYong Jun Goh, Rodolphe Barrangou · 2018 · Current Opinion in Biotechnology
- High-Throughput Approaches to Pinpoint Function within the Noncoding GenomeAntonino Montalbano, Matthew C. Canver, Neville E. Sanjana · 2017 · Molecular Cell
- Harnessing lipid nanoparticles for efficient CRISPR deliveryJingyue Yan, Diana D. Kang, Yizhou Dong · 2021 · Biomaterials Science
- Tools and targets: the dual role of plant viruses in CRISPR–Cas genome editingUranga, Mireia, Daròs, José‐Antonio · 2023 · The Plant Genome
- Advances in amplification-free detection of nucleic acid: CRISPR/Cas system as a powerful toolSiwenjie Qian, Yanju Chen, Xiaoli Xu, Cheng Peng, Xiaofu Wang, Hui Wu, Yang Liu, Xiao‐Ping Zhong, Junfeng Xu, Jian Feng Wu · 2022 · Analytical Biochemistry
- Characterization and applications of Type I CRISPR-Cas systemsClaudio Hidalgo-Cantabrana, Rodolphe Barrangou · 2020 · Biochemical Society Transactions
- Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and ProspectsHymavathi Salava, Sravankumar Thula, Vijee Mohan, Rahul Kumar, Fatemeh Maghuly · 2021 · International Journal of Molecular Sciences
- Achievements and Challenges of Genomics-Assisted Breeding in Forest Trees: From Marker-Assisted Selection to Genome EditingSunny Ahmar, Paulina Ballesta, Mohsin Ali, Freddy Mora · 2021 · International Journal of Molecular Sciences
- Effective genome editing and identification of a regiospecific gallic acid 4-O-glycosyltransferase in pomegranate (Punica granatum L.)Lijing Chang, Sheng Wu, Li Tian · 2019 · Horticulture Research
- Roles of bacteriophages, plasmids and CRISPR immunity in microbial community dynamics revealed using time-series integrated meta-omicsSusana Martínez Arbas, Shaman Narayanasamy, Malte Herold, Laura Lebrun, Michael R. Hoopmann, Sujun Li, Tony J. Lam, Benoit Josef Kunath, Nathan D. Hicks, Cindy M. Liu et al. · 2020 · Nature Microbiology
- RNA-targeting CRISPR systems from metagenomic discovery to transcriptomic engineeringAaron A. Smargon, Yilan J. Shi, Gene Wei-Ming Yeo · 2020 · Nature Cell Biology
- Futuristic CRISPR-based biosensing in the cloud and internet of things era: an overviewAbdullahi Umar Ibrahim, Fadi M. Al-Turjman, Zubaida Sa’id, Mehmet Özsöz · 2020 · Multimedia Tools and Applications
- Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activityConnor A. Tsuchida, Shouyue Zhang, Mohammad Saffari Doost, Yuqian Zhao, Jia Wang, Elizabeth O’Brien, Huan Fang, Chengping Li, Danyuan Li, Zhuo-Yan Hai et al. · 2022 · Molecular Cell
- CRISPR-Cas9-Based Technology and Its Relevance to Gene Editing in Parkinson’s DiseaseMujeeb Ur Rahman, Muhammad Bilal, Junaid Ali Shah, Ajeet Kumar Kaushik, Pierre‐Louis Teissèdre, Małgorzata Kujawska · 2022 · Pharmaceutics
- The compact Casπ (Cas12l) ‘bracelet’ provides a unique structural platform for DNA manipulation2023
- CRISPR/Cas9-gene editing approaches in plant breedingHimanshu Saini, Rajneesh Thakur, Rubina Gill, Kalpana Tyagi, Manika Goswami · 2023 · GM crops & food
- DNA silencing by prokaryotic Argonaute proteins adds a new layer of defense against invading nucleic acidsSarah Willkomm, Kira S. Makarova, Dina Grohmann · 2018 · FEMS Microbiology Reviews
- Targeted transposition with Tn7 elements: safe sites, mobile plasmids, CRISPR/Cas and beyondJoseph E. Peters · 2019 · Molecular Microbiology
- The repurposing of type I-E CRISPR-Cascade for gene activation in plantsJoshua K. Young, Stephen L. Gasior, Spencer Jones, Lijuan Wang, Pedro Navarro, Becca Vickroy, Rodolphe Barrangou · 2019 · Communications Biology
- Endogenous CRISPR-Cas System-Based Genome Editing and Antimicrobials: Review and ProspectsYingjun Li, Nan Peng · 2019 · Frontiers in Microbiology
- Advancing Mesenchymal Stem Cell Therapy with CRISPR/Cas9 for Clinical Trial StudiesAli Golchin, Forough Shams, Farshid Karami · 2019 · Advances in experimental medicine and biology
- Dairy lactococcal and streptococcal phage–host interactions: an industrial perspective in an evolving phage landscapeDennis A. Romero, Damian Magill, Anne M. Millen, Philippe Horvath, Christophe Fremaux · 2020 · FEMS Microbiology Reviews
- Novel Strategies to Combat the Emerging Drug Resistance in Human Pathogenic MicrobesHafsa Qadri, Abdul Haseeb Shah, Manzoor Ahmad Mir · 2020 · Current Drug Targets
- Functional Features and Current Applications of the RNA‐Targeting Type VI CRISPR‐Cas SystemsVanja Perčulija, Jinying Lin, Bo Zhang, Songying Ouyang · 2021 · Advanced Science
- State of the art CRISPR-based strategies for cancer diagnostics and treatmentEmma Di Carlo, Carlo Sorrentino · 2024 · Biomarker Research
- Using an Endogenous CRISPR-Cas System for Genome Editing in the Human Pathogen Clostridium difficileAnna Maikova, Victor Kreis, Anaïs Boutserin, Konstantin Severinov, Olga A. Soutourina · 2019 · Applied and Environmental Microbiology
- Novel nucleic acid detection strategies based on CRISPR‐Cas systems: From construction to applicationChushu Zhu, Chuanyang Liu, Xinyuan Qiu, Sisi Xie, Wen‐ying Li, Lingyun Zhu, Lvyun Zhu · 2020 · Biotechnology and Bioengineering
- CRISPR /Cas‐based tools for the targeted control of plant virusesGaëlle Robertson, Johan Theodorus Burger, Manuela Campa · 2022 · Molecular Plant Pathology
- An Update on the Application of CRISPR Technology in Clinical PracticeFirouzeh Morshedzadeh, Mahmoud Ghanei, Malihe Lotfi, Morteza Ghasemi, Mohsen Ahmadi, Parisa Najari-Hanjani, Samaneh Sharif, Sina Mozaffari‐Jovin, Maryam Peymani, Mohammad Reza Abbaszadegan · 2023 · Molecular Biotechnology
- Role of CRISPR-Cas systems and anti-CRISPR proteins in bacterial antibiotic resistanceHiva Kadkhoda, Pourya Gholizadeh, Hossein Samadi Kafil, Reza Ghotaslou, Tahereh Pirzadeh, Mohammad Ahangarzadeh Rezaee, Edris Nabizadeh, Hadi Feizi, Mohammad Hossein Aghazadeh · 2024 · Heliyon
- Comparative genomic analysis of Staphylococcus lugdunensis shows a closed pan-genome and multiple barriers to horizontal gene transferXavier Argemi, Dorota Matelska, Krzysztof Ginalski, Philippe Riegel, Yves Hansmann, Jochen Bloom, Martine Pestel‐Caron, Sandrine Dahyot, Jérémie Lebeurre, Gilles Prévost · 2018 · BMC Genomics
- Atypical organizations and epistatic interactions of CRISPRs and cas clusters in genomes and their mobile genetic elementsAude G. Bernheim, David Bikard, Marie Touchon, Eduardo P. C. Rocha · 2019 · Nucleic Acids Research
- Imprecise Spacer Acquisition Generates CRISPR-Cas Immune Diversity through Primed AdaptationSimon A. Jackson, Nils Birkholz, Lucía M. Malone, Peter C. Fineran · 2019 · Cell Host & Microbe
- Type I-F CRISPR-Cas resistance against virulent phages results in abortive infection and provides population-level immunityBridget N. J. Watson, Reuben B. Vercoe, George P. C. Salmond, Edze Rients Westra, Raymond H.J. Staals, Peter C. Fineran · 2019 · Nature Communications
- CRISPR-Cas gene editing technology and its application prospect in medicinal plantsMiaoxian Guo, Hongyu Chen, Shuting Dong, Zheng Zhang, Hongmei Luo · 2022 · Chinese Medicine
- Guide RNA engineering enables efficient CRISPR editing with a miniature Syntrophomonas palmitatica Cas12f1 nucleaseYujue Wang, Yannan Wang, Deng Pan, Haopeng Yu, Yifei Zhang, Weizhong Chen, Fan Li, Zhaowei Wu, Quanjiang Ji · 2022 · Cell Reports
- CRISPR Applications in Plant Virology: Virus Resistance and BeyondNatalia O. Kalinina, А. В. Хромов, Andrew J. Love, Michael E. Taliansky · 2019 · Phytopathology
- CRISPR-cas9: a powerful tool towards precision medicine in cancer treatmentHui Xing, Linghua Meng · 2019 · Acta Pharmacologica Sinica
- Exploitation of the Cooperative Behaviors of Anti-CRISPR PhagesAnne Chevallereau, Sean Meaden, Olivier Fradet, Mariann Landsberger, Alice Maestri, Ambarish Biswas, Sylvain Gandon, Stineke van Houte, Edze Rients Westra · 2019 · Cell Host & Microbe
- The rapidly advancing Class 2 CRISPR‐Cas technologies: A customizable toolbox for molecular manipulationsJingyi Wang, Chenzi Zhang, Bo Feng · 2020 · Journal of Cellular and Molecular Medicine
- CRISPR Visualizer: rapid identification and visualization of CRISPR loci via an automated high-throughput processing pipelineMatthew A. Nethery, Rodolphe Barrangou · 2018 · RNA Biology
- Tunable Repression of Key Photosynthetic Processes Using Cas12a CRISPR Interference in the Fast-Growing Cyanobacterium Synechococcus sp. UTEX 2973Cory J. Knoot, Sandeep Biswas, Himadri B. Pakrasi · 2019 · ACS Synthetic Biology
- CRISPR‐Cas12a System for Biosensing and Gene RegulationYuyan Shi, Xiaoyi Fu, Yao Yin, Fangqi Peng, Xia Yin, Guoliang Ke, Xiaobing Zhang · 2021 · Chemistry - An Asian Journal
- Perspectives on plant virus diseases in a climate change scenario of elevated temperaturesWei‐An Tsai, Christopher Andrew Brosnan, Neena Mitter, Ralf Georg Dietzgen · 2022 · Stress Biology
- Cas4 Nucleases Can Effect Specific Integration of CRISPR SpacersZhufeng Zhang, Saifu Pan, Tao Liu, Yingjun Li, Nan Peng · 2019 · Journal of Bacteriology
- Progresses, Challenges, and Prospects of Genome Editing in Soybean (Glycine max)Hu Xu, Lixiao Zhang, Kang Zhang, Yidong Ran · 2020 · Frontiers in Plant Science
- Cryo-EM structure of the RNA-guided ribonuclease Cas12gZhuang Li, Heng Zhang, Renjian Xiao, Ruijie Han, Leifu Chang · 2021 · Nature Chemical Biology
- Carbon-negative synthetic biology: challenges and emerging trends of cyanobacterial technologyChunlin Tan, Ping Xu, Fei Tao · 2022 · Trends in biotechnology
- CRISPR/Cas and Argonaute-Based Biosensors for Pathogen DetectionZhiruo Yang, Siying Mao, Lu Wang, Sinan Fu, Yanming Dong, Nicole J. Jaffrezic-Renault, Zhenzhong Guo · 2023 · ACS Sensors
- Harnessing “A Billion Years of Experimentation”: The Ongoing Exploration and Exploitation of CRISPR–Cas Immune SystemsSanne E. Klompe, Samuel H. Sternberg · 2018 · The CRISPR Journal
- A Type IV-A CRISPR-Cas System in Pseudomonas aeruginosa Mediates RNA-Guided Plasmid Interference In VivoValerie M. Crowley, Adam Catching, Hannah N. Taylor, Adair L. Borges, Josie Metcalf, Joseph Bondy‐Denomy, Ryan N. Jackson · 2019 · The CRISPR Journal
- New Insights for Biosensing: Lessons from Microbial Defense SystemsYi Wan, Chengli Zong, Xiangpeng Li, Aimin Wang, Yan Li, Tao Yang, Qing Bao, Michael S. DuBow, Mingying Yang, Rodrigo Ledesma‐Amaro et al. · 2022 · Chemical Reviews
- Protein Inhibitors of CRISPR-Cas9Joseph Bondy‐Denomy · 2017 · ACS Chemical Biology
- The ecology and evolution of microbial CRISPR-Cas adaptive immune systemsEdze Rients Westra, Stineke van Houte, Sylvain Gandon, Rachel J. Whitaker · 2019 · Philosophical Transactions of the Royal Society B Biological Sciences
- CRISPR/Cas9 Gene Editing: An Unexplored Frontier for Forest PathologyErika N. Dort, Philippe Tanguay, Richard C. Hamelin · 2020 · Frontiers in Plant Science
- Identification of a Type IV-A CRISPR-Cas System Located Exclusively on IncHI1B/IncFIB Plasmids in EnterobacteriaceaeEnas Newire, Alp Aydin, Samina Juma, Virve I. Enne, Adam Paul Roberts · 2020 · Frontiers in Microbiology
- Directed Evolution of CRISPR/Cas Systems for Precise Gene EditingRongming Liu, Liya Liang, Emily F. Freed, Ryan T. Gill · 2020 · Trends in biotechnology
- Methods and applications of CRISPR/Cas system for genome editing in stem cellsGuang Yang, Xingxu Huang · 2019 · Cell Regeneration
- A Type III-B Cmr effector complex catalyzes the synthesis of cyclic oligoadenylate second messengers by cooperative substrate bindingWenyuan Han, Stefano Stella, Yan Zhang, Tong Guo, Karolina Sulek, Li Peng-Lundgren, Guillermo Montoya, Qunxin She · 2018 · Nucleic Acids Research
- Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologsThomas L. Jacobsen, Fani Ttofali, Chunyu Liao, Srinivas Manchalu, Benjamin N. Gray, Chase L. Beisel · 2020 · Nucleic Acids Research
- Repurposing type I–F CRISPR–Cas system as a transcriptional activation tool in human cellsYuxi Chen, Jiaqi Liu, Shengyao Zhi, Qi Zheng, Wenbin Ma, Junjiu Huang, Yizhi Liu, Dan Liu, Puping Liang, Zhou Songyang · 2020 · Nature Communications
- Crop bioengineering via gene editing: reshaping the future of agricultureMohamed A. M. Atia, Wenjun Jiang, Khalid Sedeek, Haroon Butt, Magdy M. Mahfouz · 2024 · Plant Cell Reports
- Structural basis of CRISPR-Cas Type III prokaryotic defence systemsMolina, Rafael, Sofos, Nicholas, Montoya, Guillermo · 2020 · Current Opinion in Structural Biology
- Gene-Editing Technologies Paired With Viral Vectors for Translational Research Into Neurodegenerative DiseasesJoseph E. Rittiner, Malik Moncalvo, Ornit Chiba‐Falek, Boris Kantor · 2020 · Frontiers in Molecular Neuroscience
- Covalent Modifications of the Bacteriophage Genome Confer a Degree of Resistance to Bacterial CRISPR SystemsYuepeng Liu, Li Dai, Junhua Dong, Cen Chen, Jingen Zhu, Venigalla Basaveswara Rao, Tao Pan · 2020 · Journal of Virology
- Biology and applications of CRISPR–Cas12 and transposon-associated homologsWen Y. Wu, Belén Adiego-Pérez, John van der OOST · 2024 · Nature Biotechnology
- Applications of CRISPR Technologies Across the Food Supply ChainKatelyn Brandt, Rodolphe Barrangou · 2019 · Annual Review of Food Science and Technology
- Latest Advances of Virology Research Using CRISPR/Cas9-Based Gene-Editing Technology and Its Application to Vaccine DevelopmentMan Teng, Yongxiu Yao, Venugopal Nair, Jun Luo · 2021 · Viruses
- Fusing T5 exonuclease with Cas9 and Cas12a increases the frequency and size of deletion at target sitesQianwei Zhang, Kangquan Yin, Guanwen Liu, Shengnan Li, Mengou Li, Jin‐Long Qiu · 2020 · Science China Life Sciences
- CRISPR-derived genome editing technologies for metabolic engineeringKeiji Nishida, Akihiko Kondo · 2020 · Metabolic Engineering