Diversity, classification and evolution of CRISPR-Cas systems

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761

Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769

An updated evolutionary classification of CRISPR–Cas systems

Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., et al. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3569 · Cited by 2,480

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

Abudayyeh, O. O., Gootenberg, J. S., Konermann, S., Joung, J., Slaymaker, I. M., Cox, D. B. T., Shmakov, S., Makarova, K. S., Semenova, E., Minakhin, L., et al. (2016) · Science · doi:10.1126/science.aaf5573 · Cited by 2,336

Evolution and classification of the CRISPR–Cas systems

Makarova, K. S., Haft, D. H., Barrangou, R., Brouns, S. J. J., Charpentier, E., Horvath, P., Moineau, S., Mojica, F. J. M., Wolf, Y. I., Yakunin, A. F., et al. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2577 · Cited by 2,331

Crystal structure of Cas9 in complex with guide RNA and target DNA

Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

East-Seletsky, A., O’Connell, M. R., Knight, S. C., Burstein, D., Cate, J. H. D., Tjian, R., & Doudna, J. A. (2016) · Nature · doi:10.1038/nature19802 · Cited by 1,236

Structures of Cas9 endonucleases reveal RNA-mediated conformational activation

Jinek, M., Jiang, F., Taylor, D. W., Sternberg, S. H., Kaya, E., Ma, E., Anders, C., Hauer, M., Zhou, K., Lin, S., et al. (2014) · Science · doi:10.1126/science.1247997 · Cited by 1,230

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

Shmakov, S., Abudayyeh, O. O., Makarova, K. S., Wolf, Y. I., Gootenberg, J. S., Semenova, E., Minakhin, L., Joung, J., Konermann, S., Severinov, K., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.10.008 · Cited by 1,209

CRISPR delivery systems for organ-specific targeting: Advances and challenges

Du, J., Wu, Q., Liu, C., Wang, N., & Gong, C. (2025) · Precision medicine and engineering. · doi:10.1016/j.preme.2025.100048 · Cited by 20

Targeted assemblies of cas1 suggest CRISPR-Cas’s response to soil warming

Wu, R., Chai, B., Cole, J. R., Gunturu, S., Guo, X. Y., Tian, R., Gu, J., Zhou, J. Z., & Tiedje, J. M. (2020) · The ISME Journal · doi:10.1038/s41396-020-0635-1 · Cited by 15

Application of CRISPR-Cas9 System to Study Biological Barriers to Drug Delivery

He, J., Biswas, R., Bugde, P., Li, J., Liu, D., & Li, Y. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14050894 · Cited by 16

Decrypting the mechanistic basis of CRISPR/Cas9 protein

Panda, G., & Ray, A. (2022) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2022.05.001 · Cited by 17

Structural basis of AcrIF24 as an anti-CRISPR protein and transcriptional suppressor

Mukherjee, I. A., Gabel, C. A., Noinaj, N., Bondy‐Denomy, J., & Chang, L. (2022) · Nature Chemical Biology · doi:10.1038/s41589-022-01137-w · Cited by 15

Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens

Gao, H., Shang, Z., Chan, S. Y., & Ma, D. (2022) · Journal of Zhejiang University SCIENCE B · doi:10.1631/jzus.b2200068 · Cited by 15

Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector

Huo, Y., Zhao, H., Dong, Q., & Jiang, T. (2023) · Nature Microbiology · doi:10.1038/s41564-022-01316-4 · Cited by 15

The genus Arachis: an excellent resource for studies on differential gene expression for stress tolerance

Kumar, D., & Kirti, P. B. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1275854 · Cited by 16

Recent Progress in Prompt Molecular Detection of Exosomes Using CRISPR/Cas and Microfluidic‐Assisted Approaches Toward Smart Cancer Diagnosis and Analysis

Mousavi, S. M., Hashemi, S. A., Kalashgrani, M. Y., Rahmanian, V., Riazi, M., Omidifar, N., Althomali, R. H., Rahman, M. M., Chiang, W., & Gholami, A. (2023) · ChemMedChem · doi:10.1002/cmdc.202300359 · Cited by 15

The immune system of prokaryotes: potential applications and implications for gene editing

Liu, S. M., Liu, H., Wang, X., & Shi, L. (2024) · Biotechnology Journal · doi:10.1002/biot.202300352 · Cited by 16

Genomic characterisation of generic Escherichia coli from food-producing animals and products of animal origin in South Africa

Malesa, R. P., Pierneef, R., Magwedere, K., Mafuna, T., & Matle, I. (2024) · Frontiers in Bacteriology · doi:10.3389/fbrio.2024.1432292 · Cited by 16

CRISPR/Cas9-mediated editing of jasmonic acid pathways to enhance biotic & abiotic stress tolerance: An overview & prospects

Kaur, J., Ananthu Viswanathan, P., & Bari, V. K. (2025) · Functional & Integrative Genomics · doi:10.1007/s10142-025-01623-z · Cited by 15

Cross-cleavage activity of Cas6b in crRNA processing of two different CRISPR-Cas systems in Methanosarcina mazei Gö1

Nickel, L., Ulbricht, A., Alkhnbashi, O. S., Förstner, K. U., Cassidy, L., Weidenbach, K., Backofen, R., & Schmitz, R. A. (2018) · RNA Biology · doi:10.1080/15476286.2018.1514234 · Cited by 15

Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/cas3 Expression to Resist Bacteriophage

Yang, D., Wang, Z., Ma, J., Fu, Q., Wu, L., Wang, H., Wang, S., Yan, Y., & Sun, J. (2020) · Viruses · doi:10.3390/v12010090 · Cited by 15

Identification of Spacer and Protospacer Sequence Requirements in the Vibrio cholerae Type I-E CRISPR/Cas System

Bourgeois, J., Lazinski, D. W., & Camilli, A. (2020) · mSphere · doi:10.1128/msphere.00813-20 · Cited by 15

Point‐of‐Care Pathogen Detection with CRISPR‐based Programmable Nucleic Acid Binding Proteins

Dhar, B. C., Steimberg, N., & Mazzoleni, G. (2020) · ChemMedChem · doi:10.1002/cmdc.202000782 · Cited by 17

Genome editing technologies: CRISPR, LEAPER, RESTORE, ARCUT, SATI, and RESCUE

Görücü Yılmaz, Ş. (2021) · IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund · doi:10.17179/excli2020-3070

CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation

Zábrady, K., Zábrady, M., Kolesár, P., Li, A. W. H., & Doherty, A. J. (2021) · Nature Communications · doi:10.1038/s41467-021-23535-9 · Cited by 15

Tetrahedral framework nucleic acids linked CRISPR/Cas13a signal amplification system for rare tumor cell detection

Li, K., Luo, S., Guan, S., Situ, B., Wu, Y. S., Ou, Z., Tao, M., Zheng, L., & Cai, Z. (2022) · Talanta · doi:10.1016/j.talanta.2022.123531 · Cited by 15

Predicted highly derived class 1 CRISPR-Cas system in Haloarchaea containing diverged Cas5 and Cas7 homologs but no CRISPR array

Makarova, K. S., Karamycheva, S. A., Shah, S. A., Vestergaard, G., Garrett, R. A., & Koonin, E. V. (2019) · FEMS Microbiology Letters · doi:10.1093/femsle/fnz079 · Cited by 15

Direct observation of the formation of a CRISPR–Cas12a R-loop complex at the single-molecule level

Cui, Y., Tang, Y., Liang, M., Ji, Q., Zeng, Y., Chen, H., Lan, J., Jin, P. K., Wang, L., Song, G., et al. (2020) · Chemical Communications · doi:10.1039/c9cc08325a · Cited by 15

Type IV CRISPR associated RNA endonuclease Cas6 - apo form

Jackson, R. N., Warner, E. E., & Olsen, K. J. (2019) · Worldwide Protein Data Bank · doi:10.2210/pdb6njy/pdb · Cited by 15

Phage Encapsulation and Delivery Technology: A Strategy for Treating Drug-Resistant Pathogenic Microorganisms

Yang, Y., Xu, Z., Soteyome, T., Premarathna, M., Yin, X., & Liu, J. (2025) · Pharmaceuticals · doi:10.3390/ph18111688 · Cited by 17

Mechanism of primer synthesis by Primase-Polymerases

Zábrady, K., Li, A. W. H., & Doherty, A. J. (2023) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2023.102652 · Cited by 14

CRISPR/Cas, Multiomics, and RNA Interference in Virus Disease Management

Viswanath, K. K., Hamid, A., Ateka, E., & Pappu, H. R. (2023) · Phytopathology · doi:10.1094/phyto-01-23-0002-v · Cited by 14

Gene editing in common cardiovascular diseases

Lauerer, A.-M., Caravia, X. M., Maier, L. S., Chemello, F., & Lebek, S. (2024) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2024.108720 · Cited by 15

CRISPR-Cas9 system: A potent tool to fight antibiotic resistance in bacteria

Moitra, A., Chakraborty, A., & Dam, B. (2024) · The Microbe · doi:10.1016/j.microb.2024.100184 · Cited by 17

Advancing fish disease research through CRISPR-Cas genome editing: Recent developments and future perspectives

Marnis, H., & Syahputra, K. (2025) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2025.110220 · Cited by 16

Bacteriophages of Myxococcus xanthus, a Social Bacterium

Vasse, M., & Wielgoss, S. (2018) · Viruses · doi:10.3390/v10070374 · Cited by 14

Directed evolution studies of a thermophilic Type II-C Cas9

Hand, T. H., Das, A., & Li, H. (2018) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2018.10.029 · Cited by 12

Prokaryotic and viral community of the sulfate‐rich crust from Peñahueca ephemeral lake, an astrobiology analogue

Martín-Cuadrado, A.-B., Senel, E., Martínez‐García, M., Cifuentes, A., Santos, F., Almansa, C., Moreno‐Paz, M., Blanco, Y., García‐Villadangos, M., del Cura, M. Á. G., et al. (2019) · Environmental Microbiology · doi:10.1111/1462-2920.14680 · Cited by 14

Recent advances in CRISPR research

Chen, B., Niu, Y., Wang, H., Wang, K., Yang, H., & Li, W. (2020) · Protein & Cell · doi:10.1007/s13238-020-00704-y · Cited by 15

CRISPR-Cas12a-Assisted Genome Editing in Amycolatopsis mediterranei

Zhou, Y., Liu, X., Wu, J., Zhao, G., & Wang, J. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00698 · Cited by 14

CRISPR-Cas system, antibiotic resistance and virulence in bacteria: Through a common lens

Roy, S., Naha, S., Rao, A., & Basu, S. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2020.12.005 · Cited by 14

Characterization of Blf4, an Archaeal Lytic Virus Targeting a Member of the Methanomicrobiales

Weidenbach, K., Wolf, S., Kupczok, A., Kern, T., Fischer, M. A., Reetz, J., Urbańska, N., Künzel, S., Schmitz, R. A., & Rother, M. (2021) · Viruses · doi:10.3390/v13101934 · Cited by 15

Enhancement of Gene Editing and Base Editing with Therapeutic Ribonucleoproteins through In Vivo Delivery Based on Absorptive Silica Nanoconstruct

Kim, S., Jeong, Y. K., Cho, C. S., Lee, S., Sohn, C. H., Kim, J. H., Jeong, Y., Jo, D. H., Bae, S., & Lee, H. (2022) · Advanced Healthcare Materials · doi:10.1002/adhm.202201825 · Cited by 13

Intracellular signaling in CRISPR-Cas defense

Amitai, G., & Sorek, R. (2017) · Science · doi:10.1126/science.aao2210 · Cited by 14

A CRISPR‐Cas9 tool to explore the genetics of Bacillus subtilis phages

Otte, K. L., Kühne, N. M., Furrer, A. D., Lozada, L. P. B., Lutz, V. T., Schilling, T., & Hertel, R. (2020) · Letters in Applied Microbiology · doi:10.1111/lam.13349 · Cited by 14

Expansion of the CRISPR/Cas Genome-Sculpting Toolbox: Innovations, Applications and Challenges

Batool, A., Malik, F. A., & Andrabi, K. I. (2020) · Molecular Diagnosis & Therapy · doi:10.1007/s40291-020-00500-8 · Cited by 14

Optimization of specific RNA knockdown in mammalian cells with CRISPR-Cas13

Burris, B. J. D., Molina Vargas, A. M., Park, B. J., & O'Connell, M. R. (2022) · Methods · doi:10.1016/j.ymeth.2022.08.007 · Cited by 13

Cmr3 regulates the suppression on cyclic oligoadenylate synthesis by tag complementarity in a Type III-B CRISPR-Cas system

Guo, T., Zheng, F., Zeng, Z., Yang, Y., Li, Q., She, Q., & Han, W. (2019) · RNA Biology · doi:10.1080/15476286.2019.1642725 · Cited by 11

Ultrasensitive Detection of Nucleic Acid and Protein at Ambient Temperature by Using an Engineered CRISPR-Cas12a-Based Digital Assay

Cheng, Y., Guo, W., Wan, Y., Li, S., Zhou, J., & Wang, J. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.5c03865 · Cited by 12

The Recent Original Perspectives on Nonculture-Based Bacteria Detection Methods: A Comprehensive Review

Güven, E., & Azizoğlu, R. O. (2022) · Foodborne Pathogens and Disease · doi:10.1089/fpd.2021.0078 · Cited by 13

Progress of CRISPR-Cas13 Mediated Live-Cell RNA Imaging and Detection of RNA-Protein Interactions

Cao, H., Wang, Y., Zhang, N., Xia, S., Tian, P., Li, L., Du, J., & Du, Y. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.866820 · Cited by 16

Transcriptomics and RNA-Based Therapeutics as Potential Approaches to Manage SARS-CoV-2 Infection

Arriaga-Canon, C., Contreras‐Espinosa, L., Rebollar‐Vega, R., Montiel-Manríquez, R., Cedro‐Tanda, A., García-Gordillo, J. A., Álvarez-Gómez, R. M., Jiménez-Trejo, F. J., Hernández, C. C., & Herrera, L. A. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231911058 · Cited by 14

Advances and applications of CRISPR/Cas-mediated interference in Escherichia coli

Lim, X., Zhang, C., & Chen, X. (2023) · Engineering Microbiology · doi:10.1016/j.engmic.2023.100123 · Cited by 14

Comparative Probiogenomics Analysis of Limosilactobacillus fermentum 3872

Ullah, M., Rizwan, M., Han, J., Raza, A., Chen, Y.-Z., Yan, M., Ma, Y., Ni, Z., Olawale, O. A., & Chen, H. (2024) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-024-10286-4 · Cited by 13

CRISPR/CasRx-mediated resistance to Soybean mosaic virus in soybean

Gao, L., Xie, L., Xiao, Y., Cheng, X., Pu, R., Zhang, Z., Liu, Y., Gao, S., Zhang, Z., Qu, H., et al. (2024) · The Crop Journal · doi:10.1016/j.cj.2024.07.007 · Cited by 13

An improved CRISPR and CRISPR interference (CRISPRi) toolkit for engineering the model methanogenic archaeon Methanococcus maripaludis

Du, Q., Wei, Y., Zhang, L., Ren, D., Gao, J., Dong, X., Bai, L.-P., & Li, J. (2024) · Microbial Cell Factories · doi:10.1186/s12934-024-02492-0 · Cited by 13

CRISPR: New promising biotechnological tool in wastewater treatment

Bhanu, L. S. M., Kataki, S., & Chatterjee, S. (2024) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2024.107066 · Cited by 15

PAM-adjacent DNA flexibility tunes CRISPR-Cas12a off-target binding

Allen, A., Cooper, B. H., Singh, J. K., Rohs, R., & Qin, P. Z. (2025) · Scientific Reports · doi:10.1038/s41598-025-87565-9 · Cited by 17

Recent advancements in bacterial anti-phage strategies and the underlying mechanisms altering susceptibility to antibiotics

Zou, H., Huang, X., Xiao, W., He, H., Liu, S., & Zeng, H. (2025) · Microbiological Research · doi:10.1016/j.micres.2025.128107 · Cited by 13

Genetic Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Recovered From Patients in Michigan and Connecticut

Blankenship, H. M., Mosci, R. E., Phan, Q., Fontana, J., Rudrik, J. T., & Manning, S. D. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00529 · Cited by 13

Genome-editing approaches and applications: a brief review on CRISPR technology and its role in cancer

Siva, N., Gupta, S., Gupta, A., Shukla, J. N., Malik, B., & Shukla, N. (2021) · 3 Biotech · doi:10.1007/s13205-021-02680-4 · Cited by 14

Recent findings and applications of biomedical engineering for COVID-19 diagnosis: a critical review

Bui, L. M., Phùng, H. T. T., Thi, T.-T. H., Singh, V., Maurya, R., Khambhati, K., Wu, C., Uddin, M. J., Trung, D. M., & Chu, D. (2021) · Bioengineered · doi:10.1080/21655979.2021.1987821 · Cited by 16

Technical considerations towards commercialization of porcine respiratory and reproductive syndrome (PRRS) virus resistant pigs

Cigan, A. M., & Knap, P. W. (2022) · CABI Agriculture and Bioscience · doi:10.1186/s43170-022-00107-5 · Cited by 15

Phage Against the Machine: Discovery and Mechanism of Type V Anti-CRISPRs

Marino, N. D. (2023) · Journal of Molecular Biology · doi:10.1016/j.jmb.2023.168054 · Cited by 13

Applications of CRISPR technology in cellular immunotherapy

Zhou, X., Renauer, P. A., Zhou, L., Fang, S., & Chen, S. (2023) · Immunological Reviews · doi:10.1111/imr.13241 · Cited by 14

Fluorescence-based methods for measuring target interference by CRISPR–Cas systems

Phan, P. T., Schelling, M. A., Xue, C., & Sashital, D. G. (2018) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2018.10.027 · Cited by 13

CRISPR-Cas, a robust gene-editing technology in the era of modern cancer immunotherapy

Miri, S. M., Tafsiri, E., Cho, W. C. S., & Ghaemi, A. (2020) · Cancer Cell International · doi:10.1186/s12935-020-01546-8 · Cited by 15

CRISPR/Cas system-based strategies for pathogenic bacteria detection

Zheng, Y., Sun, X., Li, X., Lei, J., & Song, E. (2025) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2025.118497 · Cited by 12

Big things come in small packages: Using nanomaterials for plant genetic engineering

Leal, G. G., dos Santos, Y. B. L., Ito, M. E. Q. S., & de Souza, W. R. (2025) · Plant Nano Biology · doi:10.1016/j.plana.2025.100219 · Cited by 12

Genomic discovery and structural dissection of a novel type of polymorphic toxin system in gram-positive bacteria

Li, H., Tan, Y., & Zhang, D. (2022) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2022.08.036 · Cited by 12

Application of CRISPR/Cas9-based genome editing in ecotoxicology

Zhao, F., Ding, X., Liu, Z., Yan, X. F., Chen, Y., Jiang, Y., Chen, S., Wang, Y., Kang, T., Xie, C., et al. (2023) · Environmental Pollution · doi:10.1016/j.envpol.2023.122458 · Cited by 13

Development of a separation platform comprising magnetic beads combined with the CRISPR/Cas12a system enabling ultrasensitive and rapid detection of miRNA-21

Zhang, X., Li, H., Zhao, W., Xu, J., Wang, S., & Yu, R. (2023) · Microchimica Acta · doi:10.1007/s00604-023-06038-w · Cited by 12

Type I CRISPR-Cas-mediated microbial gene editing and regulation

Xu, Z., Chen, S., Wu, W., Wen, Y., & Cao, H. (2023) · AIMS Microbiology · doi:10.3934/microbiol.2023040 · Cited by 12

CRISPR-based gene editing in plants: Focus on reagents and their delivery tools

Ebrahimi, V., & Hashemi, A. (2024) · Bioimpacts · doi:10.34172/bi.30019 · Cited by 14

Cas1 mediates the interference stage in a phage-encoded CRISPR–Cas system

Zhang, L., Wang, H., Zeng, J., Cao, X., Gao, Z., Liu, Z., Li, F., Wang, J., Zhang, Y., Yang, M., et al. (2024) · Nature Chemical Biology · doi:10.1038/s41589-024-01659-5 · Cited by 12

Navigating the CRISPR/Cas Landscape for Enhanced Diagnosis and Treatment of Wilson’s Disease

Choi, W., Cha, S., & Kim, K. (2024) · Cells · doi:10.3390/cells13141214 · Cited by 15

CRISPR/Cas9 and its derivatives to improve crop biotic stress resistance: Current status and prospects

Chattopadhyay, R., Firdous, Z., & Bari, V. K. (2024) · Physiological and Molecular Plant Pathology · doi:10.1016/j.pmpp.2024.102482 · Cited by 12

Paper-based CRISPR-Cas diagnostics: A comprehensive review of advances and applications in disease detection

Lapee-e, V., Nuanualsuwan, S., Hongtanee, L., & Yakoh, A. (2025) · Microchemical Journal · doi:10.1016/j.microc.2025.113055 · Cited by 12

Current updates on the structural and functional aspects of the CRISPR/Cas13 system for RNA targeting and editing: A next‑generation tool for cancer management (Review)

Allemailem, K. S., Rahmani, A. H., Almansour, N. M., Aldakheel, F. M., Albalawi, G., Albalawi, G., & Khan, A. A. (2025) · International Journal of Oncology · doi:10.3892/ijo.2025.5748 · Cited by 12

Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells

Bot, J. F., Zhao, Z., Li, M., Kammeron, D., Shang, P., & Geijsen, N. (2026) · Communications Biology · doi:10.1038/s42003-026-09511-3 · Cited by 12

Understanding off-target effects through hybridization kinetics and thermodynamics

Nazipova, N. N., & Shabalina, S. A. (2019) · Cell Biology and Toxicology · doi:10.1007/s10565-019-09505-4 · Cited by 11

An overview of currently available molecular Cas-tools for precise genome modification

Kondrateva, E. V., Demchenko, A. G., Лавров, А. В., & Smirnikhina, S. (2020) · Gene · doi:10.1016/j.gene.2020.145225 · Cited by 13

Molecular basis of cyclic tetra-oligoadenylate processing by small standalone CRISPR-Cas ring nucleases

Molina, R., Garcia-Martin, R., Mendez, B. L., Jensen, A. L. G., Ciges-Tomas, J. R., Marchena-Hurtado, J., Stella, S., & Montoya, G. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac923 · Cited by 12

The effects of length and sequence of gRNA on Cas13b and Cas13d activity in vitro and in vivo

Liu, Y., Jing, P., Zhou, Y., Zhang, J., Shi, J., Zhang, M., Yang, H., & Fei, J. (2023) · Biotechnology Journal · doi:10.1002/biot.202300002 · Cited by 12

Advances in bread wheat production through CRISPR/Cas9 technology: a comprehensive review of quality and other aspects

Yiğider, E., Taşpınar, M. S., & Ağar, G. (2023) · Planta · doi:10.1007/s00425-023-04199-9 · Cited by 14

Genomic investigation of emerging zoonotic pathogen Shewanella xiamenensis

Tung, K., Wang, J.-H., & Tseng, S. (2019) · Tzu Chi Medical Journal · doi:10.4103/tcmj.tcmj_69_19 · Cited by 12

How Crisp is CRISPR? CRISPR-Cas-mediated crop improvement with special focus on nutritional traits

Kaul, T., Sony, S. K., Raman, N. M., Eswaran, M., Verma, R., Prakash, A. T., Bharti, J., Motelb, K. F. A., & Kaul, R. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818581-0.00011-5 · Cited by 12

Investigating the Genomic Background of CRISPR-Cas Genomes for CRISPR-Based Antimicrobials

Shim, H. (2022) · Evolutionary Bioinformatics · doi:10.1177/11769343221103887 · Cited by 12

A Practical Guide to Genome Editing Using Targeted Nuclease Technologies

Porter, S. N., Levine, R. M., & Pruett‐Miller, S. M. (2019) · Comprehensive physiology · doi:10.1002/cphy.c180022 · Cited by 18

Chronic In Vivo CRISPR-Cas Genome Editing: Challenges, Long-Term Safety, and Outlook

Bao, C., Channell, C. I., Tseng, Y.-H., Bailey, J., Sbaiti, N., Demirkol, A., & Tsang, S. H. (2026) · Cells · doi:10.3390/cells15020156 · Cited by 14

Molecular and Computational Strategies to Increase the Efficiency of CRISPR-Based Techniques

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