Diversity, classification and evolution of CRISPR-Cas systems

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761

Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769

An updated evolutionary classification of CRISPR–Cas systems

Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., et al. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3569 · Cited by 2,480

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

Abudayyeh, O. O., Gootenberg, J. S., Konermann, S., Joung, J., Slaymaker, I. M., Cox, D. B. T., Shmakov, S., Makarova, K. S., Semenova, E., Minakhin, L., et al. (2016) · Science · doi:10.1126/science.aaf5573 · Cited by 2,336

Evolution and classification of the CRISPR–Cas systems

Makarova, K. S., Haft, D. H., Barrangou, R., Brouns, S. J. J., Charpentier, E., Horvath, P., Moineau, S., Mojica, F. J. M., Wolf, Y. I., Yakunin, A. F., et al. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2577 · Cited by 2,331

Crystal structure of Cas9 in complex with guide RNA and target DNA

Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

East-Seletsky, A., O’Connell, M. R., Knight, S. C., Burstein, D., Cate, J. H. D., Tjian, R., & Doudna, J. A. (2016) · Nature · doi:10.1038/nature19802 · Cited by 1,236

Structures of Cas9 endonucleases reveal RNA-mediated conformational activation

Jinek, M., Jiang, F., Taylor, D. W., Sternberg, S. H., Kaya, E., Ma, E., Anders, C., Hauer, M., Zhou, K., Lin, S., et al. (2014) · Science · doi:10.1126/science.1247997 · Cited by 1,230

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

Shmakov, S., Abudayyeh, O. O., Makarova, K. S., Wolf, Y. I., Gootenberg, J. S., Semenova, E., Minakhin, L., Joung, J., Konermann, S., Severinov, K., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.10.008 · Cited by 1,209

Shooting the messenger: RNA-targetting CRISPR-Cas systems

Zhu, Y., Klompe, S. E., Vlot, M., John van der, O. O. S. T., & Staals, R. H. J. (2018) · Bioscience Reports · doi:10.1042/bsr20170788 · Cited by 30

Nucleic acid cleavage with a hyperthermophilic Cas9 from an uncultured Ignavibacterium

Schmidt, S., Yu, F. B., Blainey, P. C., May, A. P., & Quake, S. R. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1904273116 · Cited by 29

CRISPR/Cas-mediated germplasm improvement and new strategies for crop protection

Dong, G., & Fan, Z. (2024) · Crop Health · doi:10.1007/s44297-023-00020-x · Cited by 30

The evolution and history of gene editing technologies

Randhawa, S., & Sengar, S. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.002 · Cited by 31

Phage Therapy for Mycobacterium Abscessus and Strategies to Improve Outcomes

Shahraki, A. H., & Mirsaeidi, M. S. (2021) · Microorganisms · doi:10.3390/microorganisms9030596 · Cited by 28

Applications of the CRISPR-Cas system for infectious disease diagnostics

Li, P., Wang, L., Yang, J., Di, L., & Li, J. (2021) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2021.1922080 · Cited by 29

CRISPR/Cas9—A Promising Therapeutic Tool to Cure Blindness: Current Scenario and Future Prospects

Ahmad, I. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231911482 · Cited by 30

How bacteria control the CRISPR-Cas arsenal

León, L. M., Mendoza, S. D., & Bondy‐Denomy, J. (2017) · Current Opinion in Microbiology · doi:10.1016/j.mib.2017.11.005 · Cited by 29

Lactococcus lactis type III-A CRISPR-Cas system cleaves bacteriophage RNA

Millen, A. M., Samson, J. E., Tremblay, D. M., Magadán, A. H., Rousseau, G. M., Moineau, S., & Romero, D. A. (2018) · RNA Biology · doi:10.1080/15476286.2018.1502589 · Cited by 28

CRISPR-Cas9 based molecular breeding in crop plants: a review

Ikram, M., Rauf, A. W., Rao, M. J., Maqsood, M. F. K., Bakhsh, M. Z. M., Ullah, M., Batool, M., Mehran, M., & Tahira, M. (2024) · Molecular Biology Reports · doi:10.1007/s11033-023-09086-w · Cited by 29

Engineered bacteriophages: A panacea against pathogenic and drug resistant bacteria

Kakkar, A., Kandwal, G., Nayak, T., Jaiswal, L. K., Srivastava, A., & Gupta, A. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e34333 · Cited by 27

Peeling back the layers of immunogenicity in Cas9-based genomic medicine

Stigzelius, V., Cavallo, A. L., Chandode, R. K., & Nitsch, R. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.06.045 · Cited by 30

CRISPR-based therapeutic genome editing for inherited blood disorders

Levesque, S., & Bauer, D. E. (2025) · Nature Reviews Drug Discovery · doi:10.1038/s41573-025-01236-y · Cited by 31

Bacterial defense mechanisms against bacteriophages: an evolutionary arms race

Ibrahim, R., & Aranjani, J. M. (2026) · Archives of Microbiology · doi:10.1007/s00203-026-04785-x · Cited by 29

Genetic Dissection of the Type III-A CRISPR-Cas System Csm Complex Reveals Roles of Individual Subunits

Mogila, I., Kazlauskiene, M., Valinskyte, S., Tamulaitienė, G., Tamulaitis, G., & Šikšnys, V. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.02.029 · Cited by 27

In Silico Processing of the Complete CRISPR‐Cas Spacer Space for Identification of PAM Sequences

Mendoza, B. J., & Trinh, C. T. (2018) · Biotechnology Journal · doi:10.1002/biot.201700595 · Cited by 24

Strategies for Developing CRISPR‐Based Gene Editing Methods in Bacteria

Wu, Z., Wang, Y., Zhang, Y., Chen, W., Wang, Y., & Ji, Q. (2019) · Small Methods · doi:10.1002/smtd.201900560 · Cited by 26

Structural basis for inhibition of an archaeal CRISPR–Cas type I-D large subunit by an anti-CRISPR protein

Manav, M. C., van Eck, L., Lin, J., Fuglsang, A., Peng, X., & Brodersen, D. E. (2020) · Nature Communications · doi:10.1038/s41467-020-19847-x · Cited by 27

CRISPR-Cas technology a new era in genomic engineering

Parsaeimehr, A., Ebirim, R. I., & Ozbay, G. (2022) · Biotechnology Reports · doi:10.1016/j.btre.2022.e00731 · Cited by 27

AcrNET: predicting anti-CRISPR with deep learning

Li, Y., Wei, Y., Xu, S. Y., Tan, Q., Zong, L., Wang, J., Wang, Y., Chen, J., Hong, L., & Li, Y. (2023) · Bioinformatics · doi:10.1093/bioinformatics/btad259 · Cited by 28

The CRISPR conundrum: evolve and maybe die, or survive and risk stagnation

García‐Martínez, J., Maldonado, R. D., Guzman, N. M., & Mojica, F. J. M. (2018) · Microbial Cell · doi:10.15698/mic2018.06.634 · Cited by 28

Porphyromonas gingivalis and its CRISPR-Cas system

Chen, T., & Olsen, I. (2019) · Journal of Oral Microbiology · doi:10.1080/20002297.2019.1638196 · Cited by 27

New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment

Yan, B., & Liang, Y. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232415758 · Cited by 28

The CRISPR-Cas system in molecular diagnostics

Song, R., Chen, Z., Xiao, H., & Wang, H. (2024) · Clinica Chimica Acta · doi:10.1016/j.cca.2024.119820 · Cited by 27

Anti-CRISPRs on the march

Koonin, E. V., & Makarova, K. S. (2018) · Science · doi:10.1126/science.aav2440 · Cited by 26

An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system

Wandera, K. G., Collins, S. P., Wimmer, F., Marshall, R., Noireaux, V., & Beisel, C. L. (2019) · Methods · doi:10.1016/j.ymeth.2019.05.014 · Cited by 25

Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines

Vilela, J. A., Rohaim, M. A., & Munir, M. (2020) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.581504 · Cited by 29

Next-Generation Sequencing and the CRISPR-Cas Nexus: A Molecular Plant Virology Perspective

Shahid, M. S., Sattar, M. N., Iqbal, Z., Raza, A., & Al-Sadi, A. M. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.609376 · Cited by 27

Reprogramming Mycobacterium Tuberculosis CRISPR System for Gene Editing and Genome-Wide RNA Interference Screening

Rahman, K., Jamal, M., Chen, X., Zhou, W., Yang, B., Zou, Y., Xu, W., Lei, Y., Wu, C., Cao, X., et al. (2021) · Genomics Proteomics & Bioinformatics · doi:10.1016/j.gpb.2021.01.008 · Cited by 26

CRISPR/Cas System: Applications and Prospects for Maize Improvement

Jiang, Y., Sun, K., & An, X. (2022) · ACS Agricultural Science & Technology · doi:10.1021/acsagscitech.1c00253 · Cited by 27

Viral Vectors, Exosomes, and Vexosomes: Potential armamentarium for delivering CRISPR/Cas to cancer cells

Farzanehpour, M., Miri, A., Alvanegh, A. G., & Gouvarchinghaleh, H. E. (2023) · Biochemical Pharmacology · doi:10.1016/j.bcp.2023.115555 · Cited by 27

Integrons as the potential targets for combating multidrug resistance in Enterobacteriaceae using CRISPR- Cas9 technique

Shetty, V. P., Akshay, S. D., Rai, P., & Deekshit, V. K. (2023) · Journal of Applied Microbiology · doi:10.1093/jambio/lxad137 · Cited by 26

Insertion sequence transposition inactivates CRISPR-Cas immunity

Sheng, Y., Wang, H., Ou, Y., Wu, Y., Ding, W. Y., Tao, M., Lin, S., Deng, Z., Bai, L., & Kang, Q. (2023) · Nature Communications · doi:10.1038/s41467-023-39964-7 · Cited by 25

Revolutionizing Tomato Cultivation: CRISPR/Cas9 Mediated Biotic Stress Resistance

Shawky, A., Hatawsh, A., Al-Saadi, N. H., Farzan, R. A., Eltawy, N., Francis, M., Abousamra, S., Ismail, Y. Y., Attia, K. A., Fakhouri, A. S., et al. (2024) · Plants · doi:10.3390/plants13162269 · Cited by 26

CRISPR-Cas13a as a next-generation tool for rapid and precise plant RNA virus diagnostics

Karimi, M., Ghorbani, A., Niazi‎, A., Rostami, M., & Tahmasebi, A. (2025) · Plant Methods · doi:10.1186/s13007-025-01401-9 · Cited by 28

Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools

Zhu, Y., & Huang, Z. (2018) · National Science Review · doi:10.1093/nsr/nwy150 · Cited by 26

CRISPR repeat sequences and relative spacing specify DNA integration by Pyrococcus furiosus Cas1 and Cas2

Grainy, J., Garrett, S. C., Graveley, B. R., & Terns, M. P. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz548 · Cited by 25

CRISPR for Neuromuscular Disorders: Gene Editing and Beyond

Young, C. S., Pyle, A. D., & Spencer, M. J. (2019) · Physiology · doi:10.1152/physiol.00012.2019 · Cited by 25

Identification and Characterization of the CRISPR/Cas System in Staphylococcus aureus Strains From Diverse Sources

Cruz-López, E. A., Rivera, G., Cruz-Hernández, M. A., Martínez-Vázquez, A. V., Castro‐Escarpulli, G., Flores-Magallón, R., Vázquez, K., Cruz-Pulido, W. L., & Bocanegra‐García, V. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.656996 · Cited by 25

CRISPR-Cas9 and beyond: what’s next in plant genome engineering

Zess, E. K., & Begemann, M. B. (2021) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-021-10185-1 · Cited by 25

CRISPR-Cas9 Toolkit for Genome Editing in an Autotrophic CO 2 -Fixing Methanogenic Archaeon

Li, J., Zhang, L., Xu, Q., Zhang, W., Li, Z., Chen, L., & Dong, X. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.01165-22 · Cited by 25

CRISPR-Cas Genome Editing for Horticultural Crops Improvement: Advantages and Prospects

Борисовна, Р. Е., Захарченко, Н. С., Lebedev, V. G., & Shestibratov, K. A. (2022) · Horticulturae · doi:10.3390/horticulturae9010038 · Cited by 25

Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia

Johnston, C. D., Skeete, C. A., Fomenkov, A. I., Roberts, R. J., & Rittling, S. R. (2017) · PLoS ONE · doi:10.1371/journal.pone.0185234 · Cited by 25

Defining the seed sequence of the Cas12b CRISPR-Cas effector complex

Jain, I., Станиславович, М. Л., Mekler, V. M., Sitnik, V. V., Rubanova, N., Severinov, K., & Semenova, E. (2018) · RNA Biology · doi:10.1080/15476286.2018.1495492 · Cited by 25

A Reverse Transcriptase-Cas1 Fusion Protein Contains a Cas6 Domain Required for Both CRISPR RNA Biogenesis and RNA Spacer Acquisition

Mohr, G., Silas, S., Stamos, J. L., Makarova, K. S., Markham, L. M., Yao, J., Elío, P. L., Sánchez-Amat, A., Fire, A. Z., Koonin, E. V., et al. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.09.013 · Cited by 25

A Functional Mini-Integrase in a Two-Protein Type V-C CRISPR System

Wright, A. V., Wang, J. Y., Burstein, D. E., Harrington, L. B., Páez-Espino, D., Kyrpides, N. C., Iavarone, A. T., Banfield, J. F., & Doudna, J. A. (2019) · Molecular Cell · doi:10.1016/j.molcel.2018.12.015 · Cited by 25

Characterization of CRISPR‐Cas systems in Leptospira reveals potential application of CRISPR in genotyping of Leptospira interrogans

Xiao, G., Yi, Y., Che, R., Zhang, Q., Imran, M., Khan, A., Yan, J., & Lin, X. (2019) · Apmis · doi:10.1111/apm.12935 · Cited by 25

Using the Endogenous CRISPR-Cas System of Heliobacterium modesticaldum To Delete the Photochemical Reaction Center Core Subunit Gene

Baker, P. L., Orf, G. S., Kevershan, K., Pyne, M. E., Bicer, T., & Redding, K. (2019) · Applied and Environmental Microbiology · doi:10.1128/aem.01644-19 · Cited by 25

CRISPR for accelerating genetic gains in under-utilized crops of the drylands: progress and prospects

Sharma, K. K., Palakolanu, S. R., Bhattacharya, J., Shankhapal, A. R., & Bhatnagar-Mathur, P. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.999207 · Cited by 24

CRISPR-Cas13a-based detection method for avian influenza virus

Wu, Y., Zhan, J., Zhao-meng, S. H. A. N., Li, Y., Liu, Y., Li, Y., Wang, Y., Liu, Z., Wen, X., & Wang, X. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1288951 · Cited by 24

CRISPR-Cas System: A New Dawn to Combat Antibiotic Resistance

Rafiq, M. S., Shabbir, M. A. B., Raza, A., Irshad, S., Asghar, A., Maan, M. K., Gondal, M. A., & Hao, H. (2024) · BioDrugs · doi:10.1007/s40259-024-00656-3 · Cited by 24

Unity among the diverse RNA-guided CRISPR-Cas interference mechanisms

Ganguly, C., Rostami, S., Long, K., Aribam, S. D., & Rajan, R. (2024) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2024.107295 · Cited by 24

Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV)

Hu, T., Ji, Q., Ke, X., Zhou, H., Zhang, S., Ma, S., Yu, C., Ju, W., Lu, M., Lin, Y., et al. (2024) · Communications Biology · doi:10.1038/s42003-024-06537-3 · Cited by 24

CRISPR/Cas technologies for cancer drug discovery and treatment

Wang, K. C., Zheng, T., & Hubbard, B. P. (2025) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2025.02.009 · Cited by 26

The multiplexed CRISPR targeting platforms

Cao, J. P., Xiao, Q., & Yan, Q. (2018) · Drug Discovery Today Technologies · doi:10.1016/j.ddtec.2018.01.001 · Cited by 27

A novel thermal Cas12b from a hot spring bacterium with high target mismatch tolerance and robust DNA cleavage efficiency

Tian, Y., Liu, R., Xian, W.-D., Xiong, M., Xiao, M., & Li, W. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.01.079 · Cited by 25

The Basis for Natural Multiresistance to Phage in Pseudomonas aeruginosa

Pourcel, C., Midoux, C., Vergnaud, G., & Latino, L. (2020) · Antibiotics · doi:10.3390/antibiotics9060339 · Cited by 24

Potential of CRISPR/Cas system in the diagnosis of COVID-19 infection

Hillary, V. E., Savarimuthu Ignacimuthu, S. J., & Ceasar, S. A. (2021) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2021.1970535 · Cited by 26

Genome Editing Strategies to Protect Livestock from Viral Infections

Söllner, J.-H., Mettenleiter, T. C., & Petersen, B. (2021) · Viruses · doi:10.3390/v13101996 · Cited by 24

Advanced CRISPR-Cas Effector Enzyme-Based Diagnostics for Infectious Diseases, Including COVID-19

Kwon, S., & Shin, H. Y. (2021) · Life · doi:10.3390/life11121356 · Cited by 25

CRISPRimmunity: an interactive web server for CRISPR-associated Important Molecular events and Modulators Used in geNome edIting Tool identifYing

Zhou, F., Yu, X., Gan, R., Ren, K., Chen, C., Ren, C., Cui, M., Liu, Y., Gao, Y., Wang, S., et al. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad425 · Cited by 25

Natural and Engineered Guide RNA–Directed Transposition with CRISPR-Associated Tn7-Like Transposons

Hsieh, S.-C., & Peters, J. E. (2024) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-030122-041908 · Cited by 25

Cas1 and Cas2 From the Type II-C CRISPR-Cas System of Riemerella anatipestifer Are Required for Spacer Acquisition

He, Y., Wang, M., Liu, M., Huang, L. F., Liu, C., Zhang, X., Yi, H., Cheng, A., Zhu, D., Yang, Q., et al. (2018) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2018.00195 · Cited by 24

Bacterial Resistance to Phage and Its Impact on Clinical Therapy

McCallin, S. E., & Oechslin, F. (2019) · Springer eBooks · doi:10.1007/978-3-030-26736-0_3 · Cited by 24

Exploring the success of Brazilian endemic clone Pseudomonas aeruginosa ST277 and its association with the CRISPR-Cas system type I-C

Silveira, M. C., Rocha-de-Souza, C. M., Albano, R. M., Santos, I. C. D. O., & Carvalho-Assef, A. P. D. (2020) · BMC Genomics · doi:10.1186/s12864-020-6650-9 · Cited by 24

CRISPR/Cas systems combined with DNA nanostructures for biomedical applications

Sun, S., Yang, H., Wu, Z., Zhang, S., Xu, J., & Shi, P. (2024) · Chemical Communications · doi:10.1039/d4cc00290c · Cited by 23

Recent Advances in the CRISPR/Cas-Based Nucleic Acid Biosensor for Food Analysis: A Review

Sun, Y., Wen, T., Zhang, P., Wang, M., & Xu, Y. (2024) · Foods · doi:10.3390/foods13203222 · Cited by 23

CRISPR Cas systems: From bacterial defense mechanisms to revolutionary tools reshaping genetic research and translation therapeutics

Saeed, K., Ayub, F., Durrani, M. A., & Mujahid, M. (2025) · The Microbe · doi:10.1016/j.microb.2025.100344 · Cited by 26

Split technology in sensors based on CRISPR/Cas12a system

Ding, X., Lei, X., & Yu, S. (2025) · Biotechnology Advances · doi:10.1016/j.biotechadv.2025.108629 · Cited by 24

CRISPR/Cas System: An Introduction

Munawar, N., & Ahmad, A. (2021) · Springer eBooks · doi:10.1007/978-981-15-7142-8_1 · Cited by 26

CRISPR-based genome editing technology and its applications in oil crops

He, J., Zhang, K., Tang, M., Zhou, W., Chen, L., Chen, Z., & Li, M. (2021) · Oil Crop Science · doi:10.1016/j.ocsci.2021.07.002 · Cited by 24

Tips, Tricks, and Potential Pitfalls of CRISPR Genome Editing in Saccharomyces cerevisiae

Antony, J. S., Hinz, J. M., & Wyrick, J. J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.924914 · Cited by 24

Lentiviral mediated delivery of CRISPR/Cas9 reduces intraocular pressure in a mouse model of myocilin glaucoma

Patil, S., Kaipa, B. R., Ranshing, S., Sundaresan, Y., Millar, J. C., Nagarajan, B., Kiehlbauch, C. C., Zhang, Q., Jain, A., Searby, C. C., et al. (2024) · Scientific Reports · doi:10.1038/s41598-024-57286-6 · Cited by 23

Molecular basis of Gabija anti-phage supramolecular assemblies

Yang, X.-Y., Shen, Z., Xie, J., Greenwald, J., Marathe, I., Lin, Q., Xie, W. J., Wysocki, V. H., & Fu, T.-M. (2024) · Nature Structural & Molecular Biology · doi:10.1038/s41594-024-01283-w · Cited by 23

Application of the CRISPR/Cas System for Generation of Pathogen-Resistant Plants

Makarova, S. S., Хромов, А. В., Spechenkova, N. A., Taliansky, M. E., & Kalinina, N. O. (2018) · Biochemistry (Moscow) · doi:10.1134/s0006297918120131 · Cited by 23

CRISPR-sub: Analysis of DNA substitution mutations caused by CRISPR-Cas9 in human cells

Hwang, G., Yu, J., Yang, S., Son, W. J., Lim, K., Kim, H. S., Kim, J., & Bae, S. (2020) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2020.06.026 · Cited by 23

Keeping Up with RNA-Based Regulation in Bacteria: New Roles for RNA Binding Proteins

Lim, E. N. K., Sasseville, C., Carrier, M., & Massé, É. (2020) · Trends in Genetics · doi:10.1016/j.tig.2020.09.014 · Cited by 23

Synthetic biology and metabolic engineering strategies in identifying and producing plant natural products; with emphasis on the CRISPR/Cas systems

Sagharyan, M., Mohammadbagherlou, S., Samari, E., Zargar, M., Ghorbani, A., & Chen, M. (2025) · Industrial Crops and Products · doi:10.1016/j.indcrop.2025.121060 · Cited by 22

Diversity and microevolution of CRISPR loci in Helicobacter cinaedi

Tomida, J., Morita, Y., Shibayama, K., Kikuchi, K., Sawa, T., Akaike, T., & Kawamura, Y. (2017) · PLoS ONE · doi:10.1371/journal.pone.0186241 · Cited by 22

Cas3/I-C mediated target DNA recognition and cleavage during CRISPR interference are independent of the composition and architecture of Cascade surveillance complex

Nimkar, S., & Anand, B. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkz1218 · Cited by 22

Protein-Mutation-Induced Conformational Changes of the DNA and Nuclease Domain in CRISPR/Cas9 Systems by Molecular Dynamics Simulations

Ray, A., & Felice, R. D. (2020) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.9b07722 · Cited by 22

Application of CRISPR-Cas9 gene editing for congenital heart disease

Seok, H., Deng, R., Cowan, D. B., & Wang, D. (2021) · Korean Journal of Pediatrics · doi:10.3345/cep.2020.02096 · Cited by 23

Points of View on the Tools for Genome/Gene Editing

Chuang, C.-K., & Lin, W. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22189872 · Cited by 26

Divergent degeneration ofcreAantitoxin genes from minimal CRISPRs and the convergent strategy of tRNA-sequestering CreT toxins

Cheng, F., Wang, R., Yu, H., Liu, C., Yang, J., Xiang, H., & Li, M. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab821 · Cited by 19

Ligation-assisted homologous recombination enables precise genome editing by deploying both MMEJ and HDR

Zhao, Z., Shang, P., Sage, F., & Geijsen, N. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac118 · Cited by 22

Structure and mechanism of the RNA dependent RNase Cas13a from Rhodobacter capsulatus

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