Diversity, classification and evolution of CRISPR-Cas systems

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761

Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769

An updated evolutionary classification of CRISPR–Cas systems

Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., et al. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3569 · Cited by 2,480

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

Abudayyeh, O. O., Gootenberg, J. S., Konermann, S., Joung, J., Slaymaker, I. M., Cox, D. B. T., Shmakov, S., Makarova, K. S., Semenova, E., Minakhin, L., et al. (2016) · Science · doi:10.1126/science.aaf5573 · Cited by 2,336

Evolution and classification of the CRISPR–Cas systems

Makarova, K. S., Haft, D. H., Barrangou, R., Brouns, S. J. J., Charpentier, E., Horvath, P., Moineau, S., Mojica, F. J. M., Wolf, Y. I., Yakunin, A. F., et al. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2577 · Cited by 2,331

Crystal structure of Cas9 in complex with guide RNA and target DNA

Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

East-Seletsky, A., O’Connell, M. R., Knight, S. C., Burstein, D., Cate, J. H. D., Tjian, R., & Doudna, J. A. (2016) · Nature · doi:10.1038/nature19802 · Cited by 1,236

Structures of Cas9 endonucleases reveal RNA-mediated conformational activation

Jinek, M., Jiang, F., Taylor, D. W., Sternberg, S. H., Kaya, E., Ma, E., Anders, C., Hauer, M., Zhou, K., Lin, S., et al. (2014) · Science · doi:10.1126/science.1247997 · Cited by 1,230

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

Shmakov, S., Abudayyeh, O. O., Makarova, K. S., Wolf, Y. I., Gootenberg, J. S., Semenova, E., Minakhin, L., Joung, J., Konermann, S., Severinov, K., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.10.008 · Cited by 1,209

Immune-orthogonal orthologues of AAV capsids and of Cas9 circumvent the immune response to the administration of gene therapy

Moreno, A. M., Palmer, N. D., Alemán, F., Chen, G., Pla, A., Jiang, N. J., Chew, W. L., Law, M., & Mali, P. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-019-0431-2 · Cited by 132

CRISPR/Cas9‐mediated genome editing: From basic research to translational medicine

Jacinto, F. V., Link, W., & Ferreira, B. I. (2020) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.14916 · Cited by 135

Label-Free Colorimetric Method for Detection of Vibrio parahaemolyticus by Trimming the G-Quadruplex DNAzyme with CRISPR/Cas12a

Chen, X., Wang, L., He, F., Chen, G., Bai, L., He, K., Zhang, F., & Xu, X. (2021) · Analytical Chemistry · doi:10.1021/acs.analchem.1c03468 · Cited by 124

Genome editing for plant research and crop improvement

Zhan, X., Lu, Y., Zhu, J., & Botella, J. R. (2020) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13063 · Cited by 121

CRISPR-Cas systems are present predominantly on mobile genetic elements in Vibrio species

McDonald, N. D., Regmi, A., Morreale, D. P., Borowski, J. D., & Boyd, E. F. (2019) · BMC Genomics · doi:10.1186/s12864-019-5439-1 · Cited by 125

Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops

Villalobos-López, M. Á., Arroyo-Becerra, A., Quintero-Jiménez, A., & Iturriaga, G. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231912053 · Cited by 129

Engineering Phages to Fight Multidrug-Resistant Bacteria

Peng, H., Chen, I. A., & Qimron, U. (2024) · Chemical Reviews · doi:10.1021/acs.chemrev.4c00681 · Cited by 123

Cas4 Facilitates PAM-Compatible Spacer Selection during CRISPR Adaptation

Kieper, S. N., Almendros, C., Behler, J., McKenzie, R. E., Nóbrega, F. L., Haagsma, A. C., Vink, J. N. A., Hess, W. R., & Brouns, S. J. J. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.02.103 · Cited by 121

Multiplex genome editing of microorganisms using CRISPR-Cas

Adiego-Pérez, B., Randazzo, P., Daran, J.-M. G., Verwaal, R., Roubos, J. A., Daran-Lapujade, P. A. S., & John van der, O. O. S. T. (2019) · FEMS Microbiology Letters · doi:10.1093/femsle/fnz086 · Cited by 116

Duplex-Specific Nuclease-Assisted CRISPR-Cas12a Strategy for MicroRNA Detection Using a Personal Glucose Meter

Gong, S., Li, J., Pan, W., Li, N., & Tang, B. (2021) · Analytical Chemistry · doi:10.1021/acs.analchem.1c02478 · Cited by 120

Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

Mir, A., Alterman, J. F., Hassler, M. R., Debacker, A. J., Hudgens, E. D., Echeverria, D., Brodsky, M. H., Khvorova, A., Watts, J. K., & Sontheimer, E. J. (2018) · Nature Communications · doi:10.1038/s41467-018-05073-z · Cited by 112

Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems

Li, M., Gong, L., Cheng, F., Yu, H., Zhao, D., Wang, R., Wang, T., Zhang, S., Zhou, J. B., Shmakov, S., et al. (2021) · Science · doi:10.1126/science.abe5601 · Cited by 114

CRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA4 Cleavage with ApA>p Formation Terminating RNase Activity

Jia, N., Jones, R. A., Yang, G., Ouerfelli, O., & Patel, D. J. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.06.014 · Cited by 118

CRISPR Modeling and Correction of Cardiovascular Disease

Liu, N., & Olson, E. N. (2022) · Circulation Research · doi:10.1161/circresaha.122.320496 · Cited by 120

Genome editing of mutant KRAS through supramolecular polymer-mediated delivery of Cas9 ribonucleoprotein for colorectal cancer therapy

Wan, T. Y., Chen, Y., Pan, Q., Xu, X., Kang, Y., Gao, X., Huang, F., Wu, C., & Yuan, P. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.03.015 · Cited by 117

Advances in Crop Breeding Through Precision Genome Editing

Nerkar, G., Devarumath, S., Purankar, M. V., Kumar, A., Valarmathi, R., Devarumath, R. M., & Appunu, C. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.880195 · Cited by 124

Harnessing type I CRISPR–Cas systems for genome engineering in human cells

Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D., Rotstein, T., Kohrs, B., Nyer, D. B., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0310-0 · Cited by 114

RNA-Independent DNA Cleavage Activities of Cas9 and Cas12a

Sundaresan, R., Parameshwaran, H. P., Yogesha, S. D., Keilbarth, M. W., & Rajan, R. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.11.100 · Cited by 110

CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks

Cofsky, J. C., Karandur, D., Huang, C. J., Witte, I. P., Kuriyan, J., & Doudna, J. A. (2020) · eLife · doi:10.7554/elife.55143 · Cited by 108

Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins

Zhu, Y., Gao, A., Zhang, Q., Wang, Y., Han, F., Liu, S., Gao, G., Serganov, A., & Gao, P. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.01.038 · Cited by 109

Phage engineering and phage‐assisted CRISPR‐Cas delivery to combat multidrug‐resistant pathogens

Khambhati, K., Bhattacharjee, G., Gohil, N., Dhanoa, G. K., Sagona, A. P., Mani, I., Bui, N. L., Chu, D., Karapurkar, J. K., Jang, S. H., et al. (2022) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10381 · Cited by 115

The CRISPR /Cas revolution reaches the RNA world: Cas13, a new Swiss Army knife for plant biologists

Wolter, F., & Puchta, H. (2018) · The Plant Journal · doi:10.1111/tpj.13899 · Cited by 113

CRISPR/Cas9: Development and Application in Rice Breeding

Romero, F. M., & Gatica-Arias, A. (2019) · Rice Science · doi:10.1016/j.rsci.2019.08.001 · Cited by 118

G-triplex: A new type of CRISPR-Cas12a reporter enabling highly sensitive nucleic acid detection

Li, T., Hu, R., Xia, J., Xu, Z., Chen, D., Xi, J., Liu, B., Zhu, J., Li, Y., Yang, Y., et al. (2021) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2021.113292 · Cited by 110

Enhanced Cas12a editing in mammalian cells and zebrafish

Liu, P., Luk, K., Shin, M., Idrizi, F., Kwok, S. F., Roscoe, B. P., Mintzer, E., Suresh, S., Morrison, K. S., Frazão, J. B., et al. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz184 · Cited by 103

CRISPR-Cas12 and Cas13: the lesser known siblings of CRISPR-Cas9

Yan, F., Wang, W. Y., & Zhang, J. (2019) · Cell Biology and Toxicology · doi:10.1007/s10565-019-09489-1 · Cited by 109

A glance at genome editing with CRISPR–Cas9 technology

Barman, A., Deb, B., & Chakraborty, S. (2019) · Current Genetics · doi:10.1007/s00294-019-01040-3 · Cited by 107

Machine-learning approach expands the repertoire of anti-CRISPR protein families

Gussow, A. B., Park, A. E., Borges, A. L., Shmakov, S., Makarova, K. S., Wolf, Y. I., Bondy‐Denomy, J., & Koonin, E. V. (2020) · Nature Communications · doi:10.1038/s41467-020-17652-0 · Cited by 111

Genome editing of lactic acid bacteria: opportunities for food, feed, pharma and biotech

Börner, R. A., Kandasamy, V., Axelsen, A. M., Nielsen, A. T., & Bosma, E. F. (2018) · FEMS Microbiology Letters · doi:10.1093/femsle/fny291 · Cited by 115

Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

Zhang, B., Ye, Y., Ye, W., Perčulija, V., Jiang, H., Chen, Y., Li, Y., Chen, J., Lin, J., Wang, S., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-10507-3 · Cited by 108

Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing

Tang, Y., & Fu, Y. (2018) · Cell & Bioscience · doi:10.1186/s13578-018-0255-x · Cited by 121

Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays

Lee, H., Zhou, Y., Taylor, D. W., & Sashital, D. G. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.03.003 · Cited by 105

A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR/Cas12a and silver nanoclusters

Ma, L., Wang, J., Li, Y., Liao, D., Zhang, W., Han, X., & Man, S. (2022) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2022.130234 · Cited by 110

Filtration-based LAMP-CRISPR/Cas12a system for the rapid, sensitive and visualized detection of Escherichia coli O157:H7

Lee, S.-Y., & Oh, S.-W. (2022) · Talanta · doi:10.1016/j.talanta.2021.123186 · Cited by 102

Transforming plant biology and breeding with CRISPR /Cas9, Cas12 and Cas13

Schindele, P., Wolter, F., & Puchta, H. (2018) · FEBS Letters · doi:10.1002/1873-3468.13073 · Cited by 110

Pathogen detection in the CRISPR–Cas era

Sashital, D. G. (2018) · Genome Medicine · doi:10.1186/s13073-018-0543-4 · Cited by 101

CRISPR-Cas Systems and the Paradox of Self-Targeting Spacers

Wimmer, F., & Beisel, C. L. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.03078 · Cited by 105

The Rise of the CRISPR/Cpf1 System for Efficient Genome Editing in Plants

Alok, A., Sandhya, D., Jogam, P., Rodrigues, V. L., Bhati, K. K., Sharma, H., & Kumar, J. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00264 · Cited by 99

CRISPR-based gene editing technology and its application in microbial engineering

Wei, J., & Li, Y. (2023) · Engineering Microbiology · doi:10.1016/j.engmic.2023.100101 · Cited by 106

Overcoming the delivery problem for therapeutic genome editing: Current status and perspective of non-viral methods

Mashel, T. V., Tarakanchikova, Y. V., Muslimov, A. R., Zyuzin, M. V., Timin, A. S., Lepik, K. V., & Fehse, B. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120282 · Cited by 102

CRISPR-Cas13a system: A novel tool for molecular diagnostics

Zhao, L., Qiu, M., Li, X., Yang, J., & Li, J. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1060947 · Cited by 107

Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry

Rollins, M. F., Chowdhury, S., Carter, J. J., Golden, S. M., Miettinen, H. M., Santiago‐Frangos, A., Faith, D. R., Lawrence, C. M., Lander, G. C., & Wiedenheft, B. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.02.001 · Cited by 94

Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins

Lee, J., Mir, A., Edraki, A., García, B., Amrani, N., Lou, H. E., Gainetdinov, I. V., Pawluk, A., Ibraheim, R., Gao, X. D., et al. (2018) · mBio · doi:10.1128/mbio.02321-18 · Cited by 99

Novel Strategy to Combat Antibiotic Resistance: A Sight into the Combination of CRISPR/Cas9 and Nanoparticles

Wan, F., Draz, M. S., Gu, M., Yu, W., Ruan, Z., & Luo, Q. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13030352 · Cited by 106

CRISPR/Cas-Based In Vitro Diagnostic Platforms for Cancer Biomarker Detection

Gong, S., Zhang, S., Lü, F., Pan, W., Li, N., & Tang, B. (2021) · Analytical Chemistry · doi:10.1021/acs.analchem.1c02533 · Cited by 100

Functional Genomics via CRISPR–Cas

Ford, K. M., McDonald, D., & Mali, P. (2018) · Journal of Molecular Biology · doi:10.1016/j.jmb.2018.06.034 · Cited by 103

CRISPR–Cas9 System for Genome Engineering of Photosynthetic Microalgae

Patel, V., Soni, N., Prasad, V., Sapre, A. V., Dasgupta, S., & Bhadra, B. (2019) · Molecular Biotechnology · doi:10.1007/s12033-019-00185-3 · Cited by 100

CRISPRdisco: An Automated Pipeline for the Discovery and Analysis of CRISPR-Cas Systems

Crawley, A. B., Henriksen, J. R., & Barrangou, R. (2018) · The CRISPR Journal · doi:10.1089/crispr.2017.0022 · Cited by 97

Fabrication of ultrasensitive electrochemical biosensor for dengue fever viral RNA Based on CRISPR/Cpf1 reaction

Lee, Y., Choi, J., Han, H., Park, S., Park, S. Y., Park, C.-W., Baek, C., Lee, T., & Min, J. (2020) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2020.128677 · Cited by 96

LAMP-CRISPR-Cas12-based diagnostic platform for detection of Mycobacterium tuberculosis complex using real-time fluorescence or lateral flow test

Wang, Y., Li, J., Li, S., Zhu, X., Wang, X., Huang, J., Yang, X., & Tai, J. (2021) · Microchimica Acta · doi:10.1007/s00604-021-04985-w · Cited by 96

Plant Virus Vectors 3.0: Transitioning into Synthetic Genomics

Cody, W. B., & Scholthof, H. B. (2019) · Annual Review of Phytopathology · doi:10.1146/annurev-phyto-082718-100301 · Cited by 92

Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA

Liu, M., Gong, S., Yu, H., Jung, K., Johnson, K. A., & Taylor, D. W. (2020) · Nature Communications · doi:10.1038/s41467-020-17411-1 · Cited by 104

Viral vectors and extracellular vesicles: innate delivery systems utilized in CRISPR/Cas-mediated cancer therapy

Ahmadi, S. E., Soleymani, M., Shahriyary, F., Amirzargar, M. R., Mahya, O., Fattahi, D., & Safa, M. (2023) · Cancer Gene Therapy · doi:10.1038/s41417-023-00597-z · Cited by 97

Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity

Jia, N., Mo, C. Y., Wang, C., Eng, E. T., Marraffini, L. A., & Patel, D. J. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.11.007 · Cited by 94

Occurrence and Nature of Off-Target Modifications by CRISPR-Cas Genome Editing in Plants

Sturme, M. H. J., van der Berg, J. P., Bouwman, L. M. S., de Schrijver, A., de Maagd, R. A., Kleter, G. A., & Battaglia, E. (2022) · ACS Agricultural Science & Technology · doi:10.1021/acsagscitech.1c00270 · Cited by 99

CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells

Lin, P., Qin, S., Pu, Q., Wang, Z., Wu, Q., Gao, P., Schettler, J., Guo, K., Li, R., Li, G., et al. (2020) · Molecular Cell · doi:10.1016/j.molcel.2020.03.033 · Cited by 95

Listeria Phages Induce Cas9 Degradation to Protect Lysogenic Genomes

Osuna, B. A., Karambelkar, S., Mahendra, C., Christie, K. A., Garcı́a, B., Davidson, A. R., Kleinstiver, B. P., Kilcher, S., & Bondy‐Denomy, J. (2020) · Cell Host & Microbe · doi:10.1016/j.chom.2020.04.001 · Cited by 92

Cas9 Cleavage of Viral Genomes Primes the Acquisition of New Immunological Memories

Nussenzweig, P. M., McGinn, J., & Marraffini, L. A. (2019) · Cell Host & Microbe · doi:10.1016/j.chom.2019.09.002 · Cited by 89

Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering

Zheng, Y., Li, J., Wang, B., Han, J., Hao, Y., Wang, S., Ma, X., Yang, S., Ma, L., Li, Y., et al. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00062 · Cited by 92

Discovery of Diverse CRISPR-Cas Systems and Expansion of the Genome Engineering Toolbox

Koonin, E. V., Gootenberg, J. S., & Abudayyeh, O. O. (2023) · Biochemistry · doi:10.1021/acs.biochem.3c00159 · Cited by 95

One-tube RPA-CRISPR Cas12a/Cas13a rapid detection of methicillin-resistant Staphylococcus aureus

Liu, Y., Liu, H., Yu, G., Sun, W., Aizaz, M., Yang, G., & Chen, L. (2023) · Analytica Chimica Acta · doi:10.1016/j.aca.2023.341757 · Cited by 92

CRISPR/Cas-Powered Amplification-Free Detection of Nucleic Acids: Current State of the Art, Challenges, and Futuristic Perspectives

Li, Y., Liu, Y., Tang, X., Qiao, J., Kou, J., Man, S., Zhu, L., & Ma, L. (2023) · ACS Sensors · doi:10.1021/acssensors.3c01463 · Cited by 92

CRISPR–Cas13 Precision Transcriptome Engineering in Cancer

Granados-Riveron, J. T., & Aquino‐Jarquín, G. (2018) · Cancer Research · doi:10.1158/0008-5472.can-18-0785 · Cited by 92

Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6

Garcia‐Doval, C., Schwede, F., Berk, C., Rostøl, J. T., Niewoehner, O., Tejero, O., Hall, J., Marraffini, L. A., & Jínek, M. (2020) · Nature Communications · doi:10.1038/s41467-020-15334-5 · Cited by 91

Nonsense suppression therapies in human genetic diseases

Martins-Dias, P., & Romão, L. (2021) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-021-03809-7 · Cited by 93

Microfluidics: the propellant of CRISPR-based nucleic acid detection

Chen, Y., Qian, S., Yu, X., Wu, J. F., & Xu, J. (2022) · Trends in biotechnology · doi:10.1016/j.tibtech.2022.07.015 · Cited by 90

The application of DNA polymerases and Cas9 as representative of DNA-modifying enzymes group in DNA sensor design (review)

Dronina, J., Samukaitė-Bubnienė, U., & Ramanavičius, A. (2020) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2020.112867 · Cited by 88

Global phosphoproteomic analysis reveals ARMC10 as an AMPK substrate that regulates mitochondrial dynamics

Chen, Z., Lei, C., Wang, C., Li, N., Srivastava, M., Tang, M., Zhang, H., Choi, J. M., Jung, S. Y., Qin, J., et al. (2019) · Nature Communications · doi:10.1038/s41467-018-08004-0 · Cited by 86

In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway

Cai, Y., Cheng, T., Yao, Y., Li, X., Ma, Y., Li, L., Zhao, H., Bao, J., Zhang, M., Qiu, Z., et al. (2019) · Science Advances · doi:10.1126/sciadv.aav3335 · Cited by 87

Multivalent Duplexed-Aptamer Networks Regulated a CRISPR-Cas12a System for Circulating Tumor Cell Detection

Lv, Z., Wang, Q., & Yang, M. (2021) · Analytical Chemistry · doi:10.1021/acs.analchem.1c02228 · Cited by 86

A technological and regulatory outlook on CRISPR crop editing

Globus, R., & Qimron, U. (2017) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26303 · Cited by 89

Molecular architectures and mechanisms of Class 2 CRISPR-associated nucleases

Garcia‐Doval, C., & Jínek, M. (2017) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2017.10.015 · Cited by 85

Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis

Rousseau, B. A., Hou, Z., Gramelspacher, M. J., & Zhang, Y. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.01.025 · Cited by 84

Type IV CRISPR RNA processing and effector complex formation in Aromatoleum aromaticum

Özcan, A., Pausch, P., Linden, A., Wulf, A., Schühle, K., Heider, J., Urlaub, H., Heimerl, T., Bange, G., & Randau, L. (2018) · Nature Microbiology · doi:10.1038/s41564-018-0274-8 · Cited by 81

Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

Dubey, A. K., Gupta, V. K., Kujawska, M., Orive, G., Kim, N., Li, C.-Z., Mishra, Y. K., & Kaushik, A. K. (2022) · Journal of nanostructure in chemistry · doi:10.1007/s40097-022-00472-7 · Cited by 88

Chemical Modifications of CRISPR RNAs to Improve Gene-Editing Activity and Specificity

Rozners, E. (2022) · Journal of the American Chemical Society · doi:10.1021/jacs.2c02633 · Cited by 89

Anti-CRISPRdb: a comprehensive online resource for anti-CRISPR proteins

Dong, C., Hao, G., Hua, H., Liu, S., Labena, A. A., Chai, G., Huang, J. H., Rao, N., & Guo, F. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx835 · Cited by 82

Temperature-Responsive Competitive Inhibition of CRISPR-Cas9

Jiang, F., Liu, J., Osuna, B. A., Xu, M. Y., Berry, J. D., Rauch, B. J., Nogales, E., Bondy‐Denomy, J., & Doudna, J. A. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.11.016 · Cited by 83

CRISPR-cas systems based molecular diagnostic tool for infectious diseases and emerging 2019 novel coronavirus (COVID-19) pneumonia

Xiang, X., Qian, K., Zhang, Z., Lin, F., Xie, Y., Liu, Y., & Yang, Z. (2020) · Journal of drug targeting · doi:10.1080/1061186x.2020.1769637 · Cited by 85

Potato Virus Y Emergence and Evolution from the Andes of South America to Become a Major Destructive Pathogen of Potato and Other Solanaceous Crops Worldwide

Torrance, L., & Talianksy, M. E. (2020) · Viruses · doi:10.3390/v12121430 · Cited by 83

Discovery and Characterization of Cas9 Inhibitors Disseminated across Seven Bacterial Phyla

Vazquez-Uribe, R., van der Helm, E., Misiakou, M.-A., Lee, S., Kol, S., & Sommer, M. O. A. (2019) · Cell Host & Microbe · doi:10.1016/j.chom.2019.01.003 · Cited by 78

The Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation

Lee, H., Dhingra, Y., & Sashital, D. G. (2019) · eLife · doi:10.7554/elife.44248 · Cited by 82

CRISPR Tools To Control Gene Expression in Bacteria

Vigouroux, A., & Bikard, D. (2020) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00077-19 · Cited by 84

Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering: advances and prospects

Mougiakos, I., Bosma, E. F., Ganguly, J., John van der, O. O. S. T., & van Kranenburg, R. (2018) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2018.01.002 · Cited by 83

Meet the Anti-CRISPRs: Widespread Protein Inhibitors of CRISPR-Cas Systems

Hwang, S., & Maxwell, K. L. (2019) · The CRISPR Journal · doi:10.1089/crispr.2018.0052 · Cited by 81

CRISPR-Cas12-Based Rapid Authentication of Halal Food

Wu, Y., Dong, Y., Shi, Y., Yang, H., Zhang, J., Khan, M. R., Deng, S., He, G., He, Q., Lv, Y., et al. (2021) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.1c03078 · Cited by 81

Activation of Thoeris antiviral system via SIR2 effector filament assembly

Tamulaitienė, G., Sabonis, D., Sasnauskas, G., Rukšėnaitė, A., Šilanskas, A., Avraham, C., Ofir, G., Sorek, R., Zaremba, M., & Šikšnys, V. (2024) · Nature · doi:10.1038/s41586-024-07092-x · Cited by 74

CRISPR–Cas: Complex Functional Networks and Multiple Roles beyond Adaptive Immunity

Faure, G., Makarova, K. S., & Koonin, E. V. (2018) · Journal of Molecular Biology · doi:10.1016/j.jmb.2018.08.030 · Cited by 82

Keeping crispr in check: diverse mechanisms of phage-encoded anti- crisprs

Trasanidou, D., Gerós, A. S., Mohanraju, P., Nieuwenweg, A. C., Nóbrega, F. L., & Staals, R. H. J. (2019) · FEMS Microbiology Letters · doi:10.1093/femsle/fnz098 · Cited by 76

CRISPR-Cas13a Inhibits HIV-1 Infection

Yin, L., Zhao, F., Sun, H., Wang, Z., Huang, Y., Zhu, W., Xu, F., Mei, S., Liu, X., Zhang, D., et al. (2020) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2020.05.030 · Cited by 82

Genome-wide correlation analysis suggests different roles of CRISPR-Cas systems in the acquisition of antibiotic resistance genes in diverse species

Shehreen, S., Chyou, T., Fineran, P. C., & Brown, C. M. (2019) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2018.0384 · Cited by 75

Applications of CRISPR/Cas13-Based RNA Editing in Plants

Kavuri, N. R., Ramasamy, M., Qi, Y., & Mandadi, K. K. (2022) · Cells · doi:10.3390/cells11172665 · Cited by 82

Staphylococcus aureus Cas9 is a multiple-turnover enzyme

Yourik, P., Fuchs, R. T., Mabuchi, M. Y., Curcuru, J. L., & Robb, G. B. (2018) · RNA · doi:10.1261/rna.067355.118 · Cited by 75

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

Zhang, X., Shi, Y., Chen, G., Wu, D., Wu, Y., & Li, G. (2022) · Small Methods · doi:10.1002/smtd.202200794 · Cited by 83

Phage Genetic Engineering Using CRISPR–Cas Systems

Hatoum-Aslan, A. (2018) · Viruses · doi:10.3390/v10060335 · Cited by 84

The CRISPR-Cas system in Enterobacteriaceae

Medina-Aparicio, L., Dávila‐Ramos, S., Rebollar-Flores, J. E., Calva, E., & Hernández-Lucas, I. (2018) · Pathogens and Disease · doi:10.1093/femspd/fty002 · Cited by 80

Mechanisms of Type I-E and I-F CRISPR-Cas Systems in Enterobacteriaceae

Xue, C., & Sashital, D. G. (2019) · EcoSal Plus · doi:10.1128/ecosalplus.esp-0008-2018 · Cited by 77

Evaluating the Efficiency of gRNAs in CRISPR/Cas9 Mediated Genome Editing in Poplars

Brügmann, T., Deecke, K., & Fladung, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20153623 · Cited by 77

Targeted transcriptional modulation with type I CRISPR–Cas systems in human cells

Pickar‐Oliver, A., Black, J. B., Lewis, M. M., Mutchnick, K. J., Klann, T. S., Gilcrest, K. A., Sitton, M. J., Nelson, C. E., Barrera, A., Bartelt, L. C., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0235-7 · Cited by 77

CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms

Huang, Z., Fang, J., Zhou, M., Gong, Z., & Xiang, T. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1011399 · Cited by 83

Diversity and evolution of class 2 CRISPR–Cas systems

Shmakov, S., Smargon, A., Scott, D., Cox, D., Pyzocha, N., Yan, W., Abudayyeh, O. O., Gootenberg, J. S., Makarova, K. S., Wolf, Y. I., et al. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.184 · Cited by 1,029

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

Mohanraju, P., Makarova, K. S., Zetsche, B., Zhang, F., Koonin, E. V., & John van der, O. O. S. T. (2016) · Science · doi:10.1126/science.aad5147 · Cited by 692

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

Shmakov, S., Abudayyeh, O. O., Makarova, K. S., Wolf, Y. I., Gootenberg, J. S., Semenova, E., Minakhin, L., Joung, J., Konermann, S., Severinov, K., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.10.008 · Cited by 1,209

Origins and evolution of CRISPR-Cas systems

Koonin, E. V., & Makarova, K. S. (2019) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2018.0087 · Cited by 494

Different Classes of CRISPR-Cas Systems

Chaudhary, E., Chaudhary, A., Sharma, S., Tiwari, V., & Garg, M. (2024) · Springer eBooks · doi:10.1007/978-981-99-8529-6_3 · Cited by 11