Diversity, classification and evolution of CRISPR-Cas systems

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761

Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769

An updated evolutionary classification of CRISPR–Cas systems

Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., et al. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3569 · Cited by 2,480

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

Abudayyeh, O. O., Gootenberg, J. S., Konermann, S., Joung, J., Slaymaker, I. M., Cox, D. B. T., Shmakov, S., Makarova, K. S., Semenova, E., Minakhin, L., et al. (2016) · Science · doi:10.1126/science.aaf5573 · Cited by 2,336

Evolution and classification of the CRISPR–Cas systems

Makarova, K. S., Haft, D. H., Barrangou, R., Brouns, S. J. J., Charpentier, E., Horvath, P., Moineau, S., Mojica, F. J. M., Wolf, Y. I., Yakunin, A. F., et al. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2577 · Cited by 2,331

Crystal structure of Cas9 in complex with guide RNA and target DNA

Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

East-Seletsky, A., O’Connell, M. R., Knight, S. C., Burstein, D., Cate, J. H. D., Tjian, R., & Doudna, J. A. (2016) · Nature · doi:10.1038/nature19802 · Cited by 1,236

Structures of Cas9 endonucleases reveal RNA-mediated conformational activation

Jinek, M., Jiang, F., Taylor, D. W., Sternberg, S. H., Kaya, E., Ma, E., Anders, C., Hauer, M., Zhou, K., Lin, S., et al. (2014) · Science · doi:10.1126/science.1247997 · Cited by 1,230

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

Shmakov, S., Abudayyeh, O. O., Makarova, K. S., Wolf, Y. I., Gootenberg, J. S., Semenova, E., Minakhin, L., Joung, J., Konermann, S., Severinov, K., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.10.008 · Cited by 1,209

Microfluidics-Based POCT for SARS-CoV-2 Diagnostics

Yin, B., Wan, X., Sohan, A. S. M. M. F., & Lin, X. (2022) · Micromachines · doi:10.3390/mi13081238 · Cited by 38

Characterization of the self-targeting Type IV CRISPR interference system in Pseudomonas oleovorans

Guo, X., Sanchez-Londono, M., Gomes‐Filho, J. V., Hernández-Tamayo, R., Rust, S., Immelmann, L. M., Schäfer, P., Wiegel, J., Graumann, P. L., & Randau, L. (2022) · Nature Microbiology · doi:10.1038/s41564-022-01229-2 · Cited by 38

CRISPR-Cas9 mediated phage therapy as an alternative to antibiotics

Balcha, F. B., & Neja, S. A. (2023) · Animal Diseases · doi:10.1186/s44149-023-00065-z · Cited by 44

Cas13d-mediated multiplex RNA targeting confers a broad-spectrum resistance against RNA viruses in potato

Zhan, X., Liu, W., Nie, B., Zhang, F., & Zhang, J. (2023) · Communications Biology · doi:10.1038/s42003-023-05205-2 · Cited by 38

Unlocking Fungal Potential: The CRISPR-Cas System as a Strategy for Secondary Metabolite Discovery

Leal, K., Rojas, E., Madariaga-Troncoso, D., Contreras, M. J., Núñez-Montero, K., Barrientos, L., Gómez-Espinoza, O., & Iturrieta‐González, I. (2024) · Journal of Fungi · doi:10.3390/jof10110748 · Cited by 41

Research progress on the application of RPA-CRISPR/Cas12a in the rapid visual detection of pathogenic microorganisms

Ji, T., Fang, X., Gao, Y., Yu, K., & Gao, X. (2025) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2025.1640938 · Cited by 41

New and emerging uses of CRISPR/Cas9 to genetically manipulate apicomplexan parasites

Cristina, M. D., & Carruthers, V. B. (2018) · Parasitology · doi:10.1017/s003118201800001x · Cited by 39

Host and body site-specific adaptation of Lactobacillus crispatus genomes

Pan, M., Hidalgo-Cantabrana, C., & Barrangou, R. (2020) · NAR Genomics and Bioinformatics · doi:10.1093/nargab/lqaa001 · Cited by 38

CRISPR‐Cas system for biomedical diagnostic platforms

Wang, Z., & Cui, W. (2020) · Open Research (University of Surrey) · doi:10.1002/viw.20200008 · Cited by 38

Advances in the Development of Phage-Based Probes for Detection of Bio-Species

Paramasivam, K., Shen, Y., Yuan, J., Waheed, I., Mao, C., & Zhou, X. (2022) · Biosensors · doi:10.3390/bios12010030 · Cited by 41

A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs

Maxwell, C. S., Jacobsen, T. L., Marshall, R., Noireaux, V., & Beisel, C. L. (2018) · Methods · doi:10.1016/j.ymeth.2018.02.016 · Cited by 35

CRISPR‐Enabled Tools for Engineering Microbial Genomes and Phenotypes

Tarasava, K., Oh, E. J., Eckert, C. A., & Gill, R. T. (2018) · Biotechnology Journal · doi:10.1002/biot.201700586 · Cited by 37

Harnessing the type I CRISPR‐Cas systems for genome editing in prokaryotes

Xu, Z., Li, Y., Li, M., Xiang, H., & Yan, A. (2020) · Environmental Microbiology · doi:10.1111/1462-2920.15116 · Cited by 38

CRISPR/Cas13-Based Platforms for a Potential Next-Generation Diagnosis of Colorectal Cancer through Exosomes Micro-RNA Detection: A Review

Durán‐Vinet, B., Araya‐Castro, K., Calderón, J. F., Vergara, L. F., Weber, H. L., Retamales, J., Araya-Castro, P., & Leal, P. (2021) · Cancers · doi:10.3390/cancers13184640 · Cited by 39

Comparative analysis of integrative and conjugative mobile genetic elements in the genus Mesorhizobium

Colombi, E., Perry, B. J., Sullivan, J. T., Bekuma, A. A., Terpolilli, J. J., Ronson, C. W., & Ramsay, J. P. (2021) · Microbial Genomics · doi:10.1099/mgen.0.000657 · Cited by 38

Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges

Biswas, P., Anand, U., Ghorai, M., Pandey, D. K., Jha, N. K., Behl, T., Kumar, M., CHAUHAN, R., Shekhawat, M. S., & Dey, A. (2021) · Biotechnology Journal · doi:10.1002/biot.202100507 · Cited by 39

CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAsPreprint

Lee, H. J., Kim, H. J., & Lee, S. J. (2020) · Genome Research · doi:10.1101/gr.257493.119 · Cited by 37

Development and application of CRISPR-based genetic tools in Bacillus species and Bacillus phages

Song, Y., He, S., Jopkiewicz, A., Setroikromo, R., van Merkerk, R., & Quax, W. J. (2022) · Journal of Applied Microbiology · doi:10.1111/jam.15704 · Cited by 38

Non-canonical CRISPR/Cas12a-based technology: A novel horizon for biosensing in nucleic acid detection

Lei, X., Cao, S., Liu, T., Wu, Y., & Yu, S. (2024) · Talanta · doi:10.1016/j.talanta.2024.125663 · Cited by 36

The type V effectors for CRISPR/Cas-mediated genome engineering in plants

Zhang, R., Chai, N., Liu, T., Zheng, Z., Lin, Q., Xie, X., Wen, J., Yang, Z. F., Liu, Y., & Zhu, Q. (2024) · Biotechnology Advances · doi:10.1016/j.biotechadv.2024.108382 · Cited by 36

CRISPR/Cas biosensing technology: From lab assays to integrated portable devices towards wearables

Shi, R., Zhong, L., Liu, G., & Mak, W. C. (2024) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2024.117796 · Cited by 36

Traditional Strategies and Cutting-Edge Technologies Used for Plant Disease Management: A Comprehensive Overview

Akhtar, H., Usman, M. H., Binyamin, R., Hameed, A., Arshad, S. F., Aslam, H. M. U., Khan, I. A., Abbas, M., Zaki, H. E. M., Ondrašek, G., et al. (2024) · Agronomy · doi:10.3390/agronomy14092175 · Cited by 44

Overview of Phage Defense Systems in Bacteria and Their Applications

Xu, X., & Gu, P. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms252413316 · Cited by 36

A short overview of the CRISPR-Cas adaptation stage

Mosterd, C., Rousseau, G. M., & Moineau, S. (2020) · Canadian Journal of Microbiology · doi:10.1139/cjm-2020-0212 · Cited by 41

Phage Therapy: Consider the Past, Embrace the Future

Wei, J., Peng, N., Liang, Y., Keke, L., & Li, Y. (2020) · Applied Sciences · doi:10.3390/app10217654 · Cited by 36

A short overview of CRISPR-Cas technology and its application in viral disease control

Ghorbani, A., Hadifar, S., Salari, R., Izadpanah, K., Burmistrz, M., Afsharifar, A., Eskandari, M. H., Niazi, A., Denes, C. E., & Neely, G. G. (2021) · Transgenic Research · doi:10.1007/s11248-021-00247-w · Cited by 40

Coordinated regulation for nature products discovery and overproduction in Streptomyces

Zhou, Q., Ning, S., & Luo, Y. (2020) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2020.04.002 · Cited by 36

CRISPR-assisted transcription activation by phase-separation proteins

Liu, J., Chen, Y., Nong, B., Luo, X., Cui, K., Li, Z., Zhang, P., Tan, W., Yang, Y., Ma, W., et al. (2023) · Protein & Cell · doi:10.1093/procel/pwad013 · Cited by 37

CRISPR/Cas9-based genome editing: A revolutionary approach for crop improvement and global food security

Muhae‐Ud‐Din, G., Ali, F., Hameed, A., Naqvi, S. A. H., Nizamani, M. M., Jabran, M., Sarfraz, S., & Wang, Y. (2023) · Physiological and Molecular Plant Pathology · doi:10.1016/j.pmpp.2023.102191 · Cited by 44

CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks

Alves, N. S. F., Pinto, C. E. M., Távora, F. T. P. K., de Melo, B. P., Arraes, F. B. M., Lourenço‐Tessutti, I. T., de Moura, S. M., de Oliveira, A. C., Morgante, C. V., Qi, Y., et al. (2024) · Journal of Advanced Research · doi:10.1016/j.jare.2024.08.024 · Cited by 41

Comprehensive Evolutionary Analysis of the Major RNA-Induced Silencing Complex Members

Zhang, R., Jing, Y., Zhang, H., Niu, Y., Liu, C., Wang, J., Zen, K., Zhang, C., & Li, D. (2018) · Scientific Reports · doi:10.1038/s41598-018-32635-4 · Cited by 36

Insights Into Non-coding RNAs as Novel Antimicrobial Drugs

Noto, G. P. D., Molina, M. C., & Quiroga, C. (2019) · Frontiers in Genetics · doi:10.3389/fgene.2019.00057 · Cited by 35

Approaches to study CRISPR RNA biogenesis and the key players involved

Behler, J., & Hess, W. R. (2019) · Methods · doi:10.1016/j.ymeth.2019.07.015 · Cited by 38

Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4

Peng, R., Li, Z., Xu, Y. M., He, S., Peng, Q., Wu, L., Wu, Y., Qi, J., Wang, P., Shi, Y., et al. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1909400116 · Cited by 35

CRISPR/Cas9: Nature's gift to prokaryotes and an auspicious tool in genome editing

Manzoor, K. N., & Khan, A. A. (2019) · Journal of Basic Microbiology · doi:10.1002/jobm.201900420 · Cited by 38

Genomic characterization of Lactobacillus fermentum DSM 20052

Brandt, K., Nethery, M. A., O’Flaherty, S., & Barrangou, R. (2020) · BMC Genomics · doi:10.1186/s12864-020-6740-8 · Cited by 34

Unveiling Human Non-Random Genome Editing Mechanisms Activated in Response to Chronic Environmental Changes: I. Where Might These Mechanisms Come from and What Might They Have Led To?

Zamai, L. (2020) · Cells · doi:10.3390/cells9112362 · Cited by 34

Principles of Nanoparticle Design for Genome Editing in Plants

Sharma, P., & Lew, T. T. S. (2022) · Frontiers in Genome Editing · doi:10.3389/fgeed.2022.846624 · Cited by 35

A CRISPR-Cas12a-based platform for ultrasensitive rapid highly specific detection of Mycobacterium tuberculosis in clinical application

Jia, N., Wang, C.-H., Liu, X., Huang, X., Xiao, F., Fu, J. X., Sun, C., Xu, Z., Wang, G., Zhou, J., et al. (2023) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2023.1192134 · Cited by 33

Heparin Specifically Inhibits CRISPR/Cas12 Activation, Enabling Ultrasensitive Heparin Detection and Gene Editing Regulation

Cao, M., Bian, X., Ji, Z., Sohail, M., Zhang, F., Linhardt, R. J., Li, B., & Zhang, X. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.4c00403 · Cited by 34

Natural diversity of CRISPR spacers of Thermus : evidence of local spacer acquisition and global spacer exchange

Lopatina, A., Medvedeva, S., Artamonova, D., Kolesnik, M. V., Sitnik, V. V., Ispolatov, Y., & Severinov, K. (2019) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2018.0092 · Cited by 34

Dehydrated CRISPR-mediated DNA analysis for visualized animal-borne virus sensing in the unprocessed blood sample

Qian, C., Wu, H., Shi, Y., Wu, J. F., & Chen, H. (2019) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2019.127440 · Cited by 34

Characterization of a novel type III CRISPR-Cas effector provides new insights into the allosteric activation and suppression of the Cas10 DNase

Lin, J., Feng, M., Zhang, H., & She, Q. (2020) · Cell Discovery · doi:10.1038/s41421-020-0160-4 · Cited by 34

DNA interference is controlled by R-loop length in a type I-F1 CRISPR-Cas system

Tuminauskaite, D., Norkūnaitė, D., Fiodorovaite, M., Tumas, S., Songailienė, I., Tamulaitienė, G., & Šinkūnas, T. (2020) · BMC Biology · doi:10.1186/s12915-020-00799-z · Cited by 34

Potential of CRISPR/Cas system as emerging tools in the detection of viral hepatitis infection

Bahrulolum, H., Tarrahimofrad, H., Rouzbahani, F. N., Nooraei, S., Sameh, M. M., Hajizade, A., & Ahmadian, G. (2023) · Virology Journal · doi:10.1186/s12985-023-02048-5 · Cited by 33

CRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases

Xia, Y., Rao, R., Xiong, M., He, B., Zheng, B., Jia, Y., Li, Y., & Yang, Y. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.3c04363 · Cited by 33

The CRISPR-Cas13a Gemini System for noncontiguous target RNA activation

Zhao, H., Sheng, Y., Zhang, T., Zhou, S., Zhu, Y., Qian, F., Liu, M., Xu, W., Zhang, D., & Hu, J. (2024) · Nature Communications · doi:10.1038/s41467-024-47281-w · Cited by 32

Three New Cs for CRISPR: Collateral, Communicate, Cooperate

Varble, A., & Marraffini, L. A. (2019) · Trends in Genetics · doi:10.1016/j.tig.2019.03.009 · Cited by 36

Application of CRISPR/Cas9 Genome Editing System in Cereal Crops

Hillary, V. E., & Ceasar, S. A. (2019) · The Open Biotechnology Journal · doi:10.2174/1874070701913010173 · Cited by 35

Co-evolution within structured bacterial communities results in multiple expansion of CRISPR loci and enhanced immunity

Pyenson, N. C., & Marraffini, L. A. (2020) · eLife · doi:10.7554/elife.53078 · Cited by 34

Diverse Class 2 CRISPR-Cas Effector Proteins for Genome Engineering Applications

Pyzocha, N., & Chen, S. (2017) · ACS Chemical Biology · doi:10.1021/acschembio.7b00800 · Cited by 33

Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas Systems

Toro, N., Mestre, M. R., Martínez‐Abarca, F., & González-Delgado, A. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.02160 · Cited by 33

Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain

Wörle, E., Newman, A., D’Silva, J., Burgio, G., & Grohmann, D. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac767 · Cited by 32

CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering

Glinšek, K., Bozovičar, K., & Bratkovič, T. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24098144 · Cited by 35

Leveraging QM/MM and Molecular Dynamics Simulations to Decipher the Reaction Mechanism of the Cas9 HNH Domain to Investigate Off-Target Effects

Maghsoud, Y., Jayasinghe‐Arachchige, V. M., Kumari, P., Cisneros, G. A., & Liu, J. (2023) · Journal of Chemical Information and Modeling · doi:10.1021/acs.jcim.3c01284 · Cited by 27

Antimicrobial resistance (AMR) management using CRISPR-Cas based genome editing

Sen, D., & Mukhopadhyay, P. (2024) · Gene and Genome Editing · doi:10.1016/j.ggedit.2024.100031 · Cited by 37

Recent progress in molecular diagnostics: The synergy of rolling circle amplification and CRISPR/Cas systems (2018–2024) – A concise review

Zhu, Z., & Yang, L. (2024) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2024.117902 · Cited by 33

Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domains

Gu, Y., Li, H., Deep, A., Enustun, E., Zhang, D., & Corbett, K. D. (2025) · Molecular Cell · doi:10.1016/j.molcel.2024.12.004 · Cited by 32

Advancing the design and delivery of CRISPR antimicrobials

Fagen, J. R., Collias, D., Singh, A. K., & Beisel, C. L. (2017) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2017.10.001 · Cited by 32

Structural insights into the modulatory role of the accessory protein WYL1 in the Type VI-D CRISPR-Cas system

Zhang, H., Cheng, D., Li, L., Wasney, G. A., & Min, J. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz269 · Cited by 32

Anti‐CRISPR proteins targeting the CRISPR‐Cas system enrich the toolkit for genetic engineering

Liu, Q., Zhang, H., & Huang, X. (2020) · The FEBS Journal · doi:10.1111/febs.15139 · Cited by 31

Synthetic chimeric nucleases function for efficient genome editing

Liu, R., Liang, L., Freed, E. F., Chang, H., Oh, E. J., Liu, Z., Garst, A. D., Eckert, C. A., & Gill, R. T. (2019) · Nature Communications · doi:10.1038/s41467-019-13500-y · Cited by 33

Techniques for investigating lncRNA transcript functions in neurodevelopment

Srinivas, T., Soares, E. S., & Guil, S. (2023) · Molecular Psychiatry · doi:10.1038/s41380-023-02377-5 · Cited by 37

Multiplexed Detection Strategies for Biosensors Based on the CRISPR-Cas System

Wei, C., Lei, X., & Yu, S. (2024) · ACS Synthetic Biology · doi:10.1021/acssynbio.4c00161 · Cited by 31

Recent trends in bioremediation and bioaugmentation strategies for mitigation of marine based pollutants: current perspectives and future outlook

Maqsood, Q., Waseem, R., Sumrin, A., Wajid, A., Tariq, M. R., Ali, S. W., & Mahnoor, M. (2024) · Discover Sustainability · doi:10.1007/s43621-024-00607-6 · Cited by 31

Engineering CRISPR/Cas12-based biosensors: Recent advances and future perspectives

Yin, L., Zhou, C., Gao, B., Liu, F., Ma, L., & Guo, F. (2025) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2025.118296 · Cited by 31

Trends and challenges of AAV-delivered gene editing therapeutics for CNS disorders: Implications for neurodegenerative disease

Kantor, B., O’Donovan, B., & Chiba‐Falek, O. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102635 · Cited by 38

Prediction and diversity of tracrRNAs from type II CRISPR-Cas systems

Chyou, T., & Brown, C. M. (2018) · RNA Biology · doi:10.1080/15476286.2018.1498281 · Cited by 31

Molecular organization of the type II-A CRISPR adaptation module and its interaction with Cas9 via Csn2

Ka, D., Jang, D. M., Han, B. W., & Bae, E. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky702 · Cited by 33

Anti-CRISPR-Based and CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2

Mayo-Muñoz, D., He, F., Jørgensen, J. B., Madsen, P. K., Bhoobalan-Chitty, Y., & Peng, X. (2018) · Viruses · doi:10.3390/v10120695 · Cited by 29

Systematic analysis of Type I‐E Escherichia coli CRISPR‐Cas PAM sequences ability to promote interference and primed adaptation

Musharova, O. S., Sitnik, V. V., Vlot, M., Savitskaya, E. E., Datsenko, K. A., Krivoy, A., Fedorov, I., Semenova, E., Brouns, S. J. J., & Severinov, K. (2019) · Molecular Microbiology · doi:10.1111/mmi.14237 · Cited by 31

CasX: a new and small CRISPR gene-editing protein

Yang, H., & Patel, D. J. (2019) · Cell Research · doi:10.1038/s41422-019-0165-4 · Cited by 31

Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins

Qiao, J., Li, W., Lin, S., Sun, W., Ma, L., & Liu, Y. (2019) · Communications Biology · doi:10.1038/s42003-019-0402-x · Cited by 30

CRISPR/Cas technology promotes the various application of Dunaliella salina system

Feng, S., Hu, L., Zhang, Q., Zhang, F., Du, J., Liang, G., Li, A., Song, G., & Liu, Y. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-10892-6 · Cited by 31

Recent progress on rapid SARS-CoV-2/COVID-19 detection by CRISPR-Cas13-based platforms

Aquino‐Jarquín, G. (2021) · Drug Discovery Today · doi:10.1016/j.drudis.2021.06.005 · Cited by 32

CRISPR-Cas System: The Powerful Modulator of Accessory Genomes in Prokaryotes

Butiuc-Keul, A. L., Farkas, A., Carpa, R., & Iordache, D. (2021) · Microbial Physiology · doi:10.1159/000516643 · Cited by 31

A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris

Liao, X., Li, L., Jameel, A., Xing, X., & Zhang, C. (2021) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-021-11688-y · Cited by 30

Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms

Tian, S., Liu, Y., Appleton, E., Wang, H., Church, G. M., & Dong, M. (2022) · Cell Reports · doi:10.1016/j.celrep.2022.110476 · Cited by 30

CRISPR Mediated Biosensing Toward Understanding Cellular Biology and Point‐of‐Care Diagnosis

Dai, Y., Wu, Y., Liu, G., & Gooding, J. J. (2020) · Angewandte Chemie · doi:10.1002/ange.202005398 · Cited by 31

CRISPR‐Cas systems in oral microbiome: From immune defense to physiological regulation

Gong, T., Zeng, J., Tang, B., Zhou, X., & Li, Y. (2020) · Molecular Oral Microbiology · doi:10.1111/omi.12279 · Cited by 31

Recent advances in nanocomposite-based delivery systems for targeted CRISPR/Cas delivery and therapeutic genetic manipulation

Ghani, M. W., Iqbal, A., Ghani, H., Bibi, S., Wang, Z., & Pei, R. (2023) · Journal of Materials Chemistry B · doi:10.1039/d2tb02610d · Cited by 32

Nanotechnology‐based CRISPR/Cas9 delivery system for genome editing in cancer treatment

Zhou, S., Li, Y., Wu, Q., & Gong, C. (2024) · MedComm – Biomaterials and Applications · doi:10.1002/mba2.70 · Cited by 30

CRISPR-aided genome engineering for secondary metabolite biosynthesis in Streptomyces

Lee, Y., Hwang, S., Kim, W., Kim, J. H., Palsson, B. Ø., & Cho, B. (2024) · Journal of Industrial Microbiology & Biotechnology · doi:10.1093/jimb/kuae009 · Cited by 30

CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications

Burbano, D. A., Kiattisewee, C., Karanjia, A. V., Cardiff, R. A. L., Faulkner, I., Sugianto, W., & Carothers, J. M. (2024) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-100522-114706 · Cited by 34

Emerging role of nanomedicine in the treatment of neuropathic pain

Bidve, P., Prajapati, N., Kalia, K., Tekade, R. K., & Tiwari, V. (2019) · Journal of drug targeting · doi:10.1080/1061186x.2019.1587444 · Cited by 30

The effects of CRISPR-Cas9 knockout of the TGF-β1 gene on antler cartilage cells in vitro

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