The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Posttranscriptional Regulation of miRNAs Harboring Conserved Terminal Loops
Open accessAbstract
We recently found that hnRNP A1, a protein implicated in many aspects of RNA processing, acts as an auxiliary factor for the Drosha-mediated processing of a microRNA precursor, pri-miR-18a. Here, we provide the mechanism by which hnRNP A1 regulates this event. We show that hnRNP A1 binds to the loop of pri-miR-18a and induces a relaxation at the stem, creating a more favorable cleavage site for Drosha. We found that approximately 14% of all pri-miRNAs have highly conserved loops, which we predict act as landing pads for trans-acting factors influencing miRNA processing. In agreement, we show that 2'O-methyl oligonucleotides targeting conserved loops (LooptomiRs) abolish miRNA processing in vitro. Furthermore, we present evidence to support an essential role of conserved loops for pri-miRNA processing. Altogether, these data suggest the existence of auxiliary factors for the processing of specific miRNAs, revealing an additional level of complexity for the regulation of miRNA biogenesis.
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Citations
Cites
The functions of animal microRNAs
Ambros, V. (2004) · Nature · doi:10.1038/nature02871 · Cited by 10,728
Evolutionarily conserved elements in vertebrate, insect, worm, and yeast genomes
Siepel, A., Bejerano, G., Pedersen, J. S., Hinrichs, A. S., Hou, M., Rosenbloom, K., Clawson, H., Spieth, J., Hillier, L. W., Richards, S., et al. (2005) · Genome Research · doi:10.1101/gr.3715005 · Cited by 3,971 · cited 1×
“Overall conservation scores per bp based upon these alignments (calculated using phastCons) (Siepel et al., 2005) were obtained from the same source.”
MethodThe multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a
Guil, S., & Cáceres, J. F. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1250 · Cited by 567
Self-citationA Family of Human MicroRNA Genes from Miniature Inverted-Repeat Transposable Elements
Piriyapongsa, J., & Jordan, I. K. (2007) · PLoS ONE · doi:10.1371/journal.pone.0000203 · Cited by 283
Cited by
Regulation of microRNA biogenesis
Ha, M., & Kim, V. N. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3838 · Cited by 5,462
The widespread regulation of microRNA biogenesis, function and decay
Krol, J., Loedige, I., & Filipowicz, W. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2843 · Cited by 4,672
Biogenesis of small RNAs in animals
Kim, V. N., Han, J., & Siomi, M. C. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2632 · Cited by 2,814
MicroRNA: Biogenesis, Function and Role in Cancer
MacFarlane, L.-A., & R. Murphy, P. (2010) · Current Genomics · doi:10.2174/138920210793175895 · Cited by 1,584
microRNAs in action: biogenesis, function and regulation
Shang, R., Lee, S., Senavirathne, G., & Lai, E. C. (2023) · Nature Reviews Genetics · doi:10.1038/s41576-023-00611-y · Cited by 897
Metadata
- Authors
- Michlewski, G., Guil, S., Semple, C. A., & Cáceres, J. F.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2008-11
- Volume
- 32
- Issue
- 3
- Pages
- 383-393
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2045672814
- Cited by
- 335
- References
- 4
- FWCI
- 10.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
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