The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Posttranscriptional Regulation of miRNAs Harboring Conserved Terminal Loops
Open accessAbstract
We recently found that hnRNP A1, a protein implicated in many aspects of RNA processing, acts as an auxiliary factor for the Drosha-mediated processing of a microRNA precursor, pri-miR-18a. Here, we provide the mechanism by which hnRNP A1 regulates this event. We show that hnRNP A1 binds to the loop of pri-miR-18a and induces a relaxation at the stem, creating a more favorable cleavage site for Drosha. We found that approximately 14% of all pri-miRNAs have highly conserved loops, which we predict act as landing pads for trans-acting factors influencing miRNA processing. In agreement, we show that 2'O-methyl oligonucleotides targeting conserved loops (LooptomiRs) abolish miRNA processing in vitro. Furthermore, we present evidence to support an essential role of conserved loops for pri-miRNA processing. Altogether, these data suggest the existence of auxiliary factors for the processing of specific miRNAs, revealing an additional level of complexity for the regulation of miRNA biogenesis.
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Cites
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“Overall conservation scores per bp based upon these alignments (calculated using phastCons) (Siepel et al., 2005) were obtained from the same source.”
MethodThe multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a
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Self-citationA Family of Human MicroRNA Genes from Miniature Inverted-Repeat Transposable Elements
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MicroRNA: Biogenesis, Function and Role in Cancer
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microRNAs in action: biogenesis, function and regulation
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The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs
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Tissue-specific control of brain-enriched miR-7 biogenesis
Choudhury, N. R., de Lima Alves, F., de Andrés-Aguayo, L., Graf, T., Cáceres, J. F., Rappsilber, J., & Michlewski, G. (2013) · Genes & Development · doi:10.1101/gad.199190.112 · Cited by 158
Self-citationBernstein, D. L., Jiang, X., & Rom, S. (2021) · Biomedicines · doi:10.3390/biomedicines9060606 · Cited by 88
Functional Interactions Between microRNAs and RNA Binding Proteins
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KSRP, many functions for a single protein
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Metadata
- Authors
- Michlewski, G., Guil, S., Semple, C. A., & Cáceres, J. F.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2008-11
- Volume
- 32
- Issue
- 3
- Pages
- 383-393
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2045672814
- Cited by
- 335
- References
- 4
- FWCI
- 10.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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