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The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes

East-Seletsky, Alexandra, O’Connell, Mitchell R., Burstein, David, Knott, Gavin J., Doudna, Jennifer A.·2017·Molecular Cell
DOI 10.1016/j.molcel.2017.04.008
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Abstract

CRISPR adaptive immunity pathways protect prokaryotic cells against foreign nucleic acids using CRISPR RNA (crRNA)-guided nucleases. In type VI-A CRISPR-Cas systems, the signature protein Cas13a (formerly C2c2) contains two separate ribonuclease activities that catalyze crRNA maturation and ssRNA degradation. The Cas13a protein family occurs across different bacterial phyla and varies widely in both protein sequence and corresponding crRNA sequence conservation. Although grouped phylogenetically together, we show that the Cas13a enzyme family comprises two distinct functional groups that recognize orthogonal sets of crRNAs and possess different ssRNA cleavage specificities. These functional distinctions could not be bioinformatically predicted, suggesting more subtle co-evolution of Cas13a enzymes. Additionally, we find that Cas13a pre-crRNA processing is not essential for ssRNA cleavage, although it enhances ssRNA targeting for crRNAs encoded internally within the CRISPR array. We define two Cas13a protein subfamilies that can operate in parallel for RNA detection both in bacteria and for diagnostic applications.

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Research works printed but not linked: 31

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Metadata

Authors
East-Seletsky, A., O’Connell, M. R., Burstein, D., Knott, G. J., & Doudna, J. A.
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2017-05
Volume
66
Issue
3
Pages
373-383.e3
ISSN
1097-2765
DOI
10.1016/j.molcel.2017.04.008
Cited by
296
References
2
Language
en
Licence

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