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Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection
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Cas13 is unique relative to other class 2 systems due to its ability to cleave ssRNA molecules homologous to crRNA, which is complemented with non-specific cleaving of other ssRNAs, similar to the Csm6 enzyme of type III systems.
“Cas13 is unique relative to other class 2 systems due to its ability to cleave ssRNA molecules homologous to crRNA, which is complemented with non-specific cleaving of other ssRNAs, similar to the Csm6 enzyme of type III systems [57,58].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [57]
Cas13a demonstrated indiscriminate RNA cleaving ability.
“However, Cas13a demonstrated indiscriminate RNA cleaving ability [57], which could hinder its usefulness as a therapeutic agent.”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [57]
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Self-citation
Metadata
- Authors
- East-Seletsky, A., O’Connell, M. R., Knight, S. C., Burstein, D., Cate, J. H. D., Tjian, R., & Doudna, J. A.
- Venue
- Nature
- Published
- 2016-09-26
- Volume
- 538
- Issue
- 7624
- Pages
- 270-273
- ISSN
- 0028-0836
- 1476-4687
- PMC
- PMC5576363
- Cited by
- 1,236
- References
- 4
- Language
- en
- Licence
- www.springer.com/tdm (tdm)
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