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Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering

Wright, Addison V., Nuñez, James K., Doudna, Jennifer A.·2016·Cell
DOI 10.1016/j.cell.2015.12.035
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Abstract

Bacteria and archaea possess a range of defense mechanisms to combat plasmids and viral infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) systems, which provide adaptive immunity against foreign nucleic acids. CRISPR systems function by acquiring genetic records of invaders to facilitate robust interference upon reinfection. In this Review, we discuss recent advances in understanding the diverse mechanisms by which Cas proteins respond to foreign nucleic acids and how these systems have been harnessed for precision genome manipulation in a wide array of organisms.

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Metadata

Authors
Wright, A. V., Nuñez, J. K., & Doudna, J. A.
Venue
Cell
Publisher
Elsevier BV
Published
2016-01
Volume
164
Issue
1-2
Pages
29-44
ISSN
0092-8674
DOI
10.1016/j.cell.2015.12.035
Cited by
989
References
8
Language
en
Licence
Funders
US National Science Foundation (1244557)

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

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