Structural basis for plasmid restriction by SMC JET nuclease
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Abstract
DNA loop-extruding SMC complexes play crucial roles in chromosome folding and DNA immunity. Prokaryotic SMC Wadjet (JET) complexes limit the spread of plasmids through DNA cleavage, yet the mechanisms for plasmid recognition are unresolved. We show that artificial DNA circularization renders linear DNA susceptible to JET nuclease cleavage. Unlike free DNA, JET cleaves immobilized plasmid DNA at a specific site, the plasmid-anchoring point, showing that the anchor hinders DNA extrusion but not DNA cleavage. Structures of plasmid-bound JetABC reveal two presumably stalled SMC motor units that are drastically rearranged from the resting state, together entrapping a U-shaped DNA segment, which is further converted to kinked V-shaped cleavage substrate by JetD nuclease binding. Our findings uncover mechanical bending of residual unextruded DNA as molecular signature for plasmid recognition and non-self DNA elimination. We moreover elucidate key elements of SMC loop extrusion, including the motor direction and the structure of a DNA-holding state.
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Submolecular video-imaging of the Smc5/6 complex topologically bound to DNA
Umeda, K., Kurokawa, Y., Kodera, N., & Murayama, Y. (2026) · Nature Communications · doi:10.1038/s41467-026-77122-x · Cited by 2 · see citing works
Phage-encoded TelN inhibits bacterial Mre11-Rad50 nuclease to protect hairpin telomeres
Houmel, M., Pellaton, N., Anchimiuk, A., & Gruber, S. (2025) · The EMBO Journal · doi:10.1038/s44318-025-00593-z · Cited by 2 · see citing works
Self-citationMechanism of DNA entrapment by a loop-extruding Wadjet SMC motor
Roisné-Hamelin, F., Liu, H. W., Marechal, N., Uchikawa, E., Durand, A., & Gruber, S. (2025) · Molecular Cell · doi:10.1016/j.molcel.2025.09.015 · Cited by 2 · see citing works
Self-citationSMC-like Wadjet system prevents plasmid transfer into Clostridium cellulovorans
Schöllkopf, A. I., Ehrenreich, A., & Liebl, W. (2025) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-025-13551-w · Cited by 1 · see citing works
Wadjet—Keeping a watchful eye on circular DNA
Corbett, K. D., & Deep, A. (2025) · Structure · doi:10.1016/j.str.2025.08.006 · Cited by 1 · see citing works
DNA Bioconjugation with Polymer Beads Using SNAP-tag® Technology
Liu, H. W., & Gruber, S. (2025) · Methods in molecular biology · doi:10.1007/978-1-0716-5005-9_6 · Cited by 1 · see citing works
Self-citationGenomic landscape of antiviral defense systems in prokaryotes and phagesPreprint
Li, J., Gu, J., Xia, R., & Li, M. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.10.26.620412 · Cited by 1 · see citing works
Plot twists and cutting corners with atypical SMCs
Haarhuis, J. H. I., & Rowland, B. D. (2024) · Molecular Cell · doi:10.1016/j.molcel.2024.02.014
The Meeting Report on the Fifth International Conference of the Chromosomal SMC Complexes
Niki, H., & Murayama, Y. (2025) · Genes to Cells · doi:10.1111/gtc.70039
Wang, C., Duan, A.-F., Pan, D.-M., Zhao, Y.-O., Yang, J., & Sun, Y.-C. (2025) · BMC Microbiology · doi:10.1186/s12866-025-04214-x
Structural Maintenance of Chromosomes Complexes in Bacteria
Sanath-Kumar, R., Liao, Q., Ren, Z., & Wang, X. (2026) · Annual Review of Microbiology · doi:10.1146/annurev-micro-041222-034556
Himpich, S., Gaudin, T., Grass, L. M., Li, H., Loi, V. V., Chen, C., Klauck, E., Popp, P. F., Feussner, M., Kuropka, B., et al. (2026) · Cell Reports · doi:10.1016/j.celrep.2026.118021
- Authors
- Roisné-Hamelin, F., Liu, H. W., Taschner, M., Li, Y., & Gruber, S.
- Affiliations
- University of Lausanne (CH)
- Corresponding author
- Gruber, Stephan
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2024-03
- Volume
- 84
- Issue
- 5
- Pages
- 883-896.e7
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W4391486282
- Cited by
- 34
- References
- 74
- Citations by year
- 2026: 6 · 2025: 20 · 2024: 8
- FWCI
- 9.06
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Funders
- European Research Council (724482)
- European Molecular Biology Organization (ATLF 302-2022, ALTF 490-2021, E248A)
- Université de Genève
- École Polytechnique Fédérale de Lausanne
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- loop extrusion, DNA circularization, DNA kinking, DNA bending, chromosome folding
- MeSH terms
- DNA, Endonucleases, Plasmids, Prokaryotic Cells, Cell Cycle Proteins
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