Evaluation of the pre-treatment effect of Centella asiatica medicinal plants on long-term potentiation (LTP) in rat model of Alzheimer's disease
Abstract
The present study was aimed to investigate the pre-treatment effect of Centella asiatica (CeA) extract on long-term potentiation (LTP) in a rat model of Alzheimer's disease (AD). A total of 32 male Wistar rats weighing 380 ± 30 g were randomly divided into four groups (n = 8). Group 1 (C: Control): the control group. Group 2 (L: Lesion): The nucleus basalis of Meynert (NBM) of rats' brain was bilaterally destroyed by injection of Ibotenic acid. Group 3 (CeA): Animals in this group received the CeA leaf extract for only a period of six weeks. Group 4 (CeA + L): The NBM of rats was destroyed by Ibotenic acid after six weeks of a diet containing the CeA leaf extract. In all groups, LTP was recorded using the electrophysiological technique and fEPSP after high frequency stimulation (HFS). The results showed that the slope and amplitude of PS as well as the sub-curve level significantly increased in the CeA + L group compared with the L and CeA groups. The CeA extract improved and strengthened the slope, amplitude and sub-curve surface of cumulative waves in animals with NBM lesion. The results showed that administration CeA extract for six weeks before induction of NBM lesion and induction of Alzheimer could enhance memory. In other words, the CeA extract had a preventive or protective role. The present study showed that CeA had a protective role for neurons among rats with NBM lesion.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Centella asiatica reduces acetylcholinesterase level and restores cholinergic dysfunction.
“This was accomplished because CA reduced the acetylcholinesterase level while restoring cholinergic dysfunction.”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [108]
Administering Centella asiatica extract for six weeks prior to inducing nucleus basalis of Meynert lesions improves memory.
“The findings also suggested that memory could be improved by administering CA extract for six weeks prior to inducing NBM lesions and AD induction [108].”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [108]
Centella asiatica extract exhibits neuroprotective effects in rats with damaged nucleus basalis of Meynert.
“In a recent work, CA extract exhibited neuroprotective effects in rats with damaged nucleus basalis of Meynert (NBM).”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [108]
Centella asiatica administration enhances cognitive abilities in rats with impaired nucleus basalis of Meynert, regardless of dose.
“The results indicated that rats with impaired NBM had enhanced cognitive abilities when CA was administered, regardless of dose.”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [108]
Centella asiatica leaf extract exhibits anti-Alzheimer's action by ameliorating cognitive impairment.
“The extract of the leaves of Centella asiatica belonging to the family Umbelliferae exhibits anti-Alzheimer's action by ameliorating cognitive impairment.”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [108]
Cites
The Rat Brain in Stereotaxic Coordinates
Paxinos, G., & Watson, C. (1983) · Elsevier eBooks · doi:10.1016/c2009-0-63235-9 · Cited by 28,585 · see citing works
A synaptic model of memory: long-term potentiation in the hippocampus
Bliss, T. V. P., & Collingridge, G. L. (1993) · Nature · doi:10.1038/361031a0 · Cited by 11,566 · see citing works
Bliss, T. V. P., & Gardner-Medwin, A. R. (1973) · The Journal of Physiology · doi:10.1113/jphysiol.1973.sp010274 · Cited by 7,460 · see citing works
Acetylcholine as a Neuromodulator: Cholinergic Signaling Shapes Nervous System Function and Behavior
Picciotto, M. R., Higley, M. J., & Mineur, Y. S. (2012) · Neuron · doi:10.1016/j.neuron.2012.08.036 · Cited by 1,404 · see citing works
Pharmacological review on Centella asiatica: A potential herbal cure-all
Gohil, K., Patel, J. A., & Gajjar, A. (2010) · Indian Journal of Pharmaceutical Sciences · doi:10.4103/0250-474x.78519 · Cited by 666 · see citing works
Prospects for the Use of Antioxidant Therapies
Maxwell, S. R. J. (1995) · Drugs · doi:10.2165/00003495-199549030-00003 · Cited by 660 · see citing works
Targeting acetylcholinesterase and butyrylcholinesterase in dementia
Lane, R. M., Potkin, S. G., & Enz, A. (2005) · The International Journal of Neuropsychopharmacology · doi:10.1017/s1461145705005833 · Cited by 654 · see citing works
RajMohan, V, & Mohandas, E. (2007) · Indian Journal of Psychiatry · doi:10.4103/0019-5545.33264 · Cited by 342 · see citing works
Cholinergic function and Alzheimer's disease
Giacobini, E. (2003) · International Journal of Geriatric Psychiatry · doi:10.1002/gps.935 · Cited by 284 · see citing works
Veerendra Kumar, M. H., & Gupta, Y. K. (2002) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(01)00394-4 · Cited by 261 · see citing works
Veerendra Kumar, M., & Gupta, Y. (2003) · Clinical and Experimental Pharmacology and Physiology · doi:10.1046/j.1440-1681.2003.03842.x · Cited by 249 · see citing works
Acetylcholinesterase inhibitors as disease-modifying therapies for Alzheimer's disease.
Muñoz-Torrero, D. (2008) · Current Medicinal Chemistry · doi:10.2174/092986708785909067 · Cited by 241 · see citing works
Geula, C., Schatz, C., & Mesulam, M. M. (1993) · Neuroscience · doi:10.1016/0306-4522(93)90266-i · Cited by 180 · see citing works
Brenowitz, W. D., Nelson, P. T., Besser, L. M., Heller, K., & Kukull, W. A. (2015) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2015.06.028 · Cited by 174 · see citing works
Memory loss in Alzheimer’s disease: implications for development of therapeutics
Gold, C. A., & Budson, A. E. (2008) · Expert Review of Neurotherapeutics · doi:10.1586/14737175.8.12.1879 · Cited by 170 · see citing works
Centella asiatica treatment during postnatal period enhances learning and memory in mice
Rao, B. S. S., Chetana, M., & Devi, P. U. (2005) · Physiology & Behavior · doi:10.1016/j.physbeh.2005.07.019 · Cited by 148 · see citing works
Kumar, A., Prakash, A. K., & Dogra, S. (2011) · International Journal of Alzheimer s Disease · doi:10.4061/2011/347569 · Cited by 135 · see citing works
Bliss, T. V. P., & Lømo, T. (1973) · The Journal of Physiology · doi:10.1113/jphysiol.1973.sp010273 · Cited by 135 · see citing works
<i>Brahmi rasayana</i> Improves Learning and Memory in Mice
Joshi, H., & Parle, M. (2006) · Evidence-based Complementary and Alternative Medicine · doi:10.1093/ecam/nek014 · Cited by 124 · see citing works
Protective effect of tetrahydropalmatine against d-galactose induced memory impairment in rat
Qu, Z., Zhang, J., Yang, H., Huo, L., Gao, J., Chen, H., & Gao, W. (2015) · Physiology & Behavior · doi:10.1016/j.physbeh.2015.11.016 · Cited by 122 · see citing works
Long-term potentiation as synaptic dialogue
Colley, P. A., & Routtenberg, A. (1993) · Brain Research Reviews · doi:10.1016/0165-0173(93)90009-o · Cited by 121 · see citing works
Normal induction but accelerated decay of LTP in APP + PS1 transgenic mice
Gureviciene, I., Ikonen, S., Gurevicius, K., Sarkaki, A., Groen, T. V., Pussinen, R., Ylinen, A., & Tanila, H. (2004) · Neurobiology of Disease · doi:10.1016/j.nbd.2003.11.011 · Cited by 115 · see citing works
Soumyanath, A., Zhong, Y.-P., Henson, E., Wadsworth, T. L., Bishop, J., Gold, B. G., & Quinn, J. F. (2012) · International Journal of Alzheimer s Disease · doi:10.1155/2012/381974 · Cited by 112 · see citing works
Acetylcholine esterase activity in mild cognitive impairment and Alzheimer’s disease
Herholz, K. (2008) · European Journal of Nuclear Medicine and Molecular Imaging · doi:10.1007/s00259-007-0699-4 · Cited by 87 · see citing works
Gray, N. E., Zweig, J. A., Caruso, M., Zhu, J. Y., Wright, K. M., Quinn, J. F., & Soumyanath, A. (2018) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2018.09.002 · Cited by 85 · see citing works
Cholinesterase Inhibitors Stabilize Alzheimer Disease
Giacobini, E. (2000) · Neurochemical Research · doi:10.1023/a:1007679709322 · Cited by 78 · see citing works
The neuroenergetics of stress hormones in the hippocampus and implications for memory
Osborne, D. M., Pearson-Leary, J., & McNay, E. C. (2015) · Frontiers in Neuroscience · doi:10.3389/fnins.2015.00164 · Cited by 78 · see citing works
Yang, W., Han, H., Hu, X., Feng, G.-F., & Qian, Y. (2013) · Pharmacology Biochemistry and Behavior · doi:10.1016/j.pbb.2013.10.027 · Cited by 76 · see citing works
Nalini, K., Ar, A., Rao, A., & Karanth, K. S. (1992) · Fitoterapia · Cited by 68 · see citing works
Effect of Drugs upon Axoplasmic Transport
McClure, W. O. (1972) · Advances in pharmacology · doi:10.1016/s1054-3589(08)60524-4 · Cited by 61 · see citing works
Gadahad, M. R. K., Rao, M. S. R., & Rao, G. S. (2008) · Journal of the Chinese Medical Association · doi:10.1016/s1726-4901(08)70066-2 · Cited by 51 · see citing works
Pisani, L., Iacobazzi, R. M., Catto, M., Rullo, M., Farina, R., Denora, N., Cellamare, S., & Altomare, C. (2018) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2018.10.016 · Cited by 51 · see citing works
Amjad, S. (2015) · Journal of Molecular Biomarkers & Diagnosis · doi:10.4172/2155-9929.1000212 · Cited by 47 · see citing works
Atukeren, P., Cengız, M., Yavuzer, H., Gelışgen, R., Altunoğlu, E., Öner, S., Erdenen, F., Yuceakın, D., Derici, H., Çakatay, U., et al. (2017) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2017.03.101 · Cited by 45 · see citing works
Doulah, A., Rohani, A. H., Khaksari, M., Motamedi, F., Farbood, Y., Badavi, M., Malek, M., & Sarkaki, A. (2009) · Neuroscience Letters · doi:10.1016/j.neulet.2009.09.016 · Cited by 21 · see citing works
Self-citationDrug regulation & therapeutic efficacy
Ritter, J. M. (2008) · British Journal of Clinical Pharmacology · doi:10.1111/j.1365-2125.2008.03208.x · Cited by 8 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Biswas, D., Mandal, S., Chatterjee Saha, S., Tudu, C. K., Nandy, S., Batiha, G. E., Shekhawat, M. S., Pandey, D. K., & Dey, A. (2021) · Phytotherapy Research · doi:10.1002/ptr.7248 · Cited by 83 · see citing works
Phytotherapy: A promising approach for the treatment of Alzheimer's disease
John, O. O., Amarachi, I. S., Chinazom, A. P., Adaeze, E., Kale, M. B., Umare, M. D., & Upaganlawar, A. B. (2022) · Pharmacological Research - Modern Chinese Medicine · doi:10.1016/j.prmcm.2021.100030 · Cited by 69 · see citing works
Nagori, K., Nakhate, K. T., Yadav, K., Ajazuddin, & Pradhan, M. (2023) · Future Pharmacology · doi:10.3390/futurepharmacol3040053 · Cited by 61 · see citing works
Transcriptome analysis identifies the role of Class I histone deacetylase in Alzheimer’s disease
Geng, F., Zhao, N., Xiu, C., Liu, X.-T., Zhu, M., Jiang, Y., & Ren, Q. (2023) · Heliyon · doi:10.1016/j.heliyon.2023.e18008 · Cited by 15 · see citing works
Sharma, D., Rajbongshi, B., Isphak, T., Basumatary, S., Dutta, K., Rudrapal, M., & Goswami, A. K. (2025) · Chemistry & Biodiversity · doi:10.1002/cbdv.202500038 · Cited by 5 · see citing works
Singh, J., Singh, A., Sharma, V., Singh, T. D., Singh, M., Garg, R., Sharma, R., & Gautam, D. N. S. (2022) · Current Pharmacology Reports · doi:10.1007/s40495-022-00300-0 · Cited by 3 · see citing works
Hosseini, R., Mirshekar, M. A., Sedaghat, G., Clark, C. C. T., & Jalali, M. (2022) · Shiraz E-Medical Journal · doi:10.5812/semj-113268 · Cited by 1 · see citing works
“The hippocampus is a segment of the temporal memory lobe implicated in the pathology of AD (5), whilst its synaptic communication is related to the formation of long-term potentiation (LTP).”
BackgroundNatural Herbs Polishing Memory: Neuroprotection against Alzheimer's Disease
Thakkur, M., Dilnashin, H., & Keshri, P. K. (2023) · BENTHAM SCIENCE PUBLISHERS eBooks · doi:10.2174/9789815040197123010017 · Cited by 1 · see citing works
Herbs for Alzheimer’s disease management—Preclinical studies: A narrative
Gorji, N., Moeini, R., & Memariani, Z. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-90052-2.00023-8 · Cited by 1 · see citing works
Non-transgenic rodent models of Alzheimer’s disease for preclinical research: a review
Joshi, A., Lehene, S., & Mishra, A. (2025) · Molecular Biology Reports · doi:10.1007/s11033-025-10549-5 · Cited by 1 · see citing works
“Through the investigation of these path - ways in a range of animal models, scientists hope to find new targets for treatment and ways to avoid AD. hippocampal AChE levels, and Aβ plaques are to be evalu - ated in rodents [ 54 ].”
Uso da infusão de folhas de Sonchus oleraceus para o tratamento de vitiligo
Eibel, G. S. B., Zilly, A., da Silva, R. M. M., & Ferreira, H. (2021) · Research Society and Development · doi:10.33448/rsd-v10i4.13824
Nutri Ayur in Cognition and Memory Dysfunction
Rahmatkar, S. N., Kumar, R., & Singh, D. (2025) · Springer eBooks · doi:10.1007/978-981-96-2960-2_9
Padma, K. R., Don, K. R., Sankari, M., Harathi, K., & Sandhya, N. (2026) · doi:10.1002/9781394360260.ch15
Данукало, М. В., Usenko, D. L., Каплаушенко, А. Г., & Kolesnyk, Y. M. (2026) · The Ukrainian Biochemical Journal · doi:10.15407/ubj98.03.044
- Authors
- Doulah, A., Mahmoodi, G., & Pourmahdi Borujeni, M.
- Affiliations
- Islamic Azad University, Ahvaz Branch (IR)
- Islamic Azad University of Kermanshah (IR)
- Corresponding author
- Doulah, Abdolhassan
- ORCID
- Mahmoodi, Gelavij
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2020-06
- Volume
- 729
- Pages
- 135026
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W3021038704
- Cited by
- 14
- References
- 36
- Citations by year
- 2026: 2 · 2025: 3 · 2023: 4 · 2022: 2 · 2021: 3
- FWCI
- 2.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ahvaz Branch, Islamic Azad University
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- Centella asiatica, Alzheimer's disease, long-term potentiation, ibotenic acid, pretreatment, memory enhancement, neuroprotection, field excitatory postsynaptic potentials, plant extracts
- MeSH terms
- Alzheimer Disease, Animals, Disease Models, Animal, Hippocampus, Ibotenic Acid, Male, Memory, Neurons, Plants, Medicinal, Rats, Wistar, Long-Term Potentiation, Basal Nucleus of Meynert
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
