Functional Connectivity and Selective Odor Responses of Excitatory Local Interneurons in Drosophila Antennal Lobe
Open accessAbstract
Local interneurons in the Drosophila antennal lobe are thought to play important roles in shaping odor responses. However, the physiological properties of excitatory local interneurons (eLNs) and their connectivity in the antennal lobe remain unclear. We first characterized the firing patterns of krasavietz-Gal4-labeled eLNs (krasavietz eLNs) in response to depolarizing currents. Paired recordings of krasavietz eLNs and PNs showed reciprocal excitatory connections mediated by dendrodendritic cholinergic synapses and gap junctions. Reciprocal connections were also found between two krasavietz eLNs but were rare between krasavietz eLNs and inhibitory LNs. Analysis of response onset latencies showed that krasavietz eLNs received monosynaptic inputs from ORNs. Furthermore, each eLN responded with distinct patterns to different odors, and each odor elicited distinct responses in different eLNs, with specific temporal patterns of spiking, indicating that eLNs serve specific coding functions in addition to global excitation in Drosophila olfactory processing.
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The remainder of the lLN2T_abc neurons is most likely GABAergic, e.g., lLN2_d gross morphology looks more like related GABAergic neurons.
“The remainder is most likely GABAergic, e.g., lLN2_d gross morphology looks more like related GABAergic neurons.63,189”
From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [189]
Literature expected perhaps as many as approximately 15 cholinergic local neurons.
“We also expected perhaps as many as ∼ 15 cholinergic local neurons.63,189”
From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [189]
The predicted cholinergic local neurons in 'ALl1 dorsal' are unilateral and pan-glomerular, in accordance with previous descriptions but more numerous.
“The predicted cholinergic neurons are unilateral and ‘broad’, i.e., pan-glomerular, in accordance with previous descriptions63,189 but more numerous.”
From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [189]
Among the lLN2T_abc neurons are approximately 13 neurons that have a cell body placement and general morphology most similar to reported cholinergic local neurons.
“Specifically, among them are ∼ 13 lLN2T_abc neurons that have a cell body placement and general morphology most similar to reported cholinergic local neurons.63,189”
From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [189]
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- Authors
- Huang, J., Zhang, W., Qiao, W., Hu, A., & Wang, Z.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Wang, Zuoren
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2010-09
- Volume
- 67
- Issue
- 6
- Pages
- 1021-1033
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W1986154944
- Cited by
- 148
- References
- 28
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 10 · 2023: 13 · 2022: 11 · 2021: 6 · 2020: 5 · 2019: 7 · 2018: 6 · 2017: 4 · 2016: 6 · 2015: 13 · 2014: 12 · 2013: 18 · 2012: 17
- FWCI
- 6.43
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 1050 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
- Keywords
- antennal lobe, Drosophila olfaction, olfactory response, functional connectivity, cholinergic synapses, gap junctions, dendrodendritic synapses, odor coding
- MeSH terms
- Action Potentials, Adenosine Triphosphate, Animals, Bungarotoxins, Drosophila, Electric Stimulation, Female, Gene Expression Regulation, Interneurons, Lysine, Mecamylamine, Nerve Net, Odorants, Olfactory Pathways, Organophosphorus Compounds, Picrotoxin, Sense Organs, Smell, Gap Junctions, Receptors, Purinergic P2, Patch-Clamp Techniques, Cholinergic Antagonists, Nicotinic Antagonists, GABA Antagonists, Drosophila Proteins, Animals, Genetically Modified, Eukaryotic Initiation Factor-5, Green Fluorescent Proteins, Inhibitory Postsynaptic Potentials, Receptors, Purinergic P2X2
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